Wound Healing Assay Kit | 고마바이오텍

특징

 

• 세포의 이동, 증식, 상처 회복 능력을 측정하기 위해 사용됩니다.
  
• “wound field” insert를 장착 후 cell을 monolayer로 배양 후, insert를 제거했을 때 세포들의 이동능력과 wound gap을 closure 하는 능력을 관찰합니다. 
• 불활성 물질을 사용함으로써 세포의 증식 혹은 이동을 방해하는 잔류물을 남기지 않습니다.
• 각 well마다 일관된 결과를 얻을 수 있습니다.
  

구성

 

• 24-well Wound Healing Assay Plate
• Cell Stain Solution
• DAPI Fluorescence Stain (1000X)
• Fixing Solution

원리

• 0.9mm 간격으로 상처 필드를 생성하는 insert를 이용하여 세포의 이동 및 증식 속도를 측정합니다.

사용 결과

Percent Closure of MEF/STO Cells. STO cells were tested using the CytoSelect™ 24-Well Wound Healing Assay. 

Cells were cultured 24 hours until a monolayer formed at which time the inserts were removed to begin the wound healing assay. 

Cells were monitored under phase contrast (not shown), DAPI labeling, and cell staining for determining percent closure (0, 50, 75, and 100%). 

사용 논문

CBA-120 CBA-120-5 CBA-120-T

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