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Leukocyte-Endothelium Adhesion Assay Kit 이미지

Leukocyte-Endothelium Adhesion Assay Kit

CytoSelect™ Leukocyte-Endothelium Adhesion Assay Kit는 내피세포를 투과한 leukocyte를 측정합니다.

프린트 제품 문의

특징

 

  • 혈관내피와 백혈구 또는 종양 세포의 상호 작용은 세포가 내피세포의 접착분자 (endothelial cell adhesion molecule)에 부착하는 일련의 과정으로 구성됩니다.
  • CytoSelect™ Leukocyte Endothelium Adhesion Assay는 백혈구와 내피 사이의 상호작용을 정량적으로 측정하기 위한 강력한 시스템을 제공합니다.
  • 부착 세포는 형광 플레이트 판독기에서 쉽게 정량화할 수 있습니다.
  • 내피 세포들을 activator나 inhibitor로 처리후 배양하고, leukocyte를 LeukoTracker로 라벨링하여 cell에 처리하면 내피세포를 투과한 leukocyte의 수를 정량할 수 있습니다.






원리

  

  • 내피세포를 배양하여 monolayer를 형성하고, 원하는 activator나 inhibitor로 처리합니다.  백혈구는 LeukoTracker™로 표지된 후 pellet, wash, resuspend 됩니다. 백혈구를 내피세포 monolayer에 추가하고 배양한 후 부착된 백혈구를 세척, 용해 후 fluorometer로 정량합니다. 

   

 


 

 

 

구성

 

  • 500 X LeukoTracker™ Solution
  • Gelatin Solution
  • 4 X Lysis Buffer
  • 10 X Wash Buffer
  • TNFα 


실험 결과


Quantitation of Human Monocytic THP-1. LeukoTracker™ labeled THP-1 cells were titrated in 1X PBS, then subsequently lysed with 2X Lysis Buffer  (75 µL of cell suspension was mixed with 75 µL of 2X Lysis Buffer). Fluorescence was quantified as described in Assay Protocol.

 
Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in 48-well plate was treated with 1 µM PMA for 12 hrs. LeukoTracker™ labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.




Cytokine Activation of Human Monocytic THP-1 Adhesion to HUVEC. HUVEC monolayer (left) or THP-1 cells (right) were treated with 50 ng/mL TNFα  or 1 µM PMA for 12 hrs. LeukoTracker™ labeled THP-1 cells (50,000 cells/well) were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.




사용 논문

CBA-210 　
    1. Bravo, P.L.W. et a. (2021). Antithrombotic Effect of the Ethanol Extract of Angelica gigas Nakai (AGE 232). Life (Basel). 11(9):939. doi: 10.3390/life11090939.
    2. Poursaleh, A. et al. (2021). Adhesion of monocytes and endothelial cells isolated from the human aorta suppresses by miRNA-PEI particles. BMC Cardiovasc Disord. 21(1):395. doi: 10.1186/s12872-021-02203-2.
    3. Gao, X. et al. (2021). Atherogenic oxoaldehyde of cholesterol induces innate immune response in monocytes and macrophages. Cell Biochem Biophys. doi: 10.1007/s12013-021-01004-w.
    4. Johnson, M.D. et al. (2020). CC16 Binding to α4β1 Integrin (VLA-4) Protects Against Mycoplasma pneumoniae Infection. Am J Respir Crit Care Med. doi: 10.1164/rccm.202006-2576OC.
    5. Guo, J. et al. (2020). Hsa-miR-23a-3p promotes atherogenesis in a novel mouse model of atherosclerosis. J Lipid Res. doi: 10.1194/jlr.RA120001121.
    6. Nair, M. et al. (2020). Enhancing Antitumor Efficacy of Heavily Vascularized Tumors by RAMBO Virus through Decreased Tumor Endothelial Cell Activation. Cancers (Basel). 12(4). pii: E1040. doi: 10.3390/cancers12041040.
    7. Sasaki, N. et al. (2020). Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy. Cell Commun Signal. 18(1):43. doi: 10.1186/s12964-020-00533-w.
    8. Liang, J. et al. (2020). Attenuation of pristimerin on TNF-α-induced endothelial inflammation. Int Immunopharmacol. 82:106326. doi: 10.1016/j.intimp.2020.106326.
    9. Abu El-Asrar, A.M. et al. (2019). Galectin-1 studies in proliferative diabetic retinopathy. Acta Ophthalmol. doi: 10.1111/aos.14191.
    10. Li, R. et al. (2018). Attenuation of antimalarial agent hydroxychloroquine on TNF-α-induced endothelial inflammation. Int Immunopharmacol. 63:261-269. doi: 10.1016/j.intimp.2018.08.008.
    11. Hong, B. et al. (2018). Suppression of HMGB1 Released in the Glioblastoma Tumor Microenvironment Reduces Tumoral Edema. Mol Ther Oncolytics. 12:93-102. doi: 10.1016/j.omto.2018.11.005.
    12. Huang, W. et al. (2018). Oridonin inhibits vascular inflammation by blocking NF-κB and MAPK activation. Eur J Pharmacol. 826:133-139. doi: 10.1016/j.ejphar.2018.02.044.
    13. Ramella, M. et al. (2017). Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA). Am J Transl Res. 9(12):5485-5495.
    14. Wojkowska, D.J. et al. (2017). Interleukin 17A Promotes Lymphocytes Adhesion and Induces CCL2 and CXCL1 Release from Brain Endothelial Cells. Int. J. Mol. Sci. doi: 10.3390/ijms18051000.
    15. Rossignoli, A. et al. (2017). Poliovirus Receptor-Related 2: A Cholesterol-Responsive Gene Affecting Atherosclerosis Development by Modulating Leukocyte Migration. Arterioscler Thromb Vasc Biol. 37(3):534-542. doi: 10.1161/ATVBAHA.116.308715.
    16. He, M. et al. (2016). miR-483 targeting of CTGF suppresses endothelial-to-mesenchymal transition: therapeutic implications in Kawasaki Disease. Circ. Res. doi:10.1161/CIRCRESAHA.116.310233.
    17. Cao, Q. et al. (2016). Myeloid deletion of α1AMPK exacerbates atherosclerosis in LDL receptor knockout (LDLRKO) mice. Diabetes. 65:1565-1576.
    18. Huang, M. et al. (2015). Niclosamide inhibits the inflammatory and angiogenic activation of human umbilical vein endothelial cells. Inflamm Res. 64:1023-1032.
    19. Shah, D. et al. (2015). C1q deficiency promotes pulmonary vascular inflammation and enhances the susceptibility of the lung endothelium to injury. J Biol Chem. doi:10.1074/jbc.M115.690784.
    20. Campos-Estrada, C. et al. (2015). Simvastatin and benznidazole-mediated prevention of Trypanosoma cruzi-induced endothelial activation: role of 15-epi-lipoxin A4 in the action of simvastatin. 9:e0003770.
    21. Ibrahim, A. S. et al. (2015). A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase. J Lipid Res. 56:599-611.
    22. Cao, Q. et al. (2014). Inhibiting DNA Methylation by 5-Aza-2'-deoxycytidine ameliorates atherosclerosis through suppressing macrophage inflammation.Endocrinology. 155:4925-4938.
    23. Cary, L. H. et al. (2014). Interactions between endothelial cells and T cells modulate responses to mixed neutron/gamma radiation. Radiat Res. 181:592-604.
    24. Burrows, G. G. et al. (2014). Human multipotent adult progenitor cells transcriptionally regulate fucosyltransferase VII. Cytotherapy. 16:566-575.
    25. Kapitsinou, P. P. et al. (2014). Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury. J Clin Invest. 124:2396.
    26. Wu, X. Y. et al. (2014). Regulation of microRNA-155 in endothelial inflammation by targeting nuclear factor (NF)-κB p65.  J Cell Biochem. 115:1928-1936.
    27. Ghoshal, P. et al. (2014). Glycosylation inhibitors efficiently inhibit P-selectin-mediated cell adhesion to endothelial cells. PLoS One. 9:e99363.
    28. Sharma-Walia, N. et al. (2014). The Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Induced 5-Lipoxygenase-Leukotriene B4 Cascade Plays Key Roles in KSHV Latency, Monocyte Recruitment, and Lipogenesis. J. Virol. 88:2131-2156.
    29. Avan, A. et al. (2013). Norepinephrine Increases NADPH Oxidase-Derived Superoxide in Human Peripheral Blood Mononuclear Cells via α-Adrenergic Receptors. Cancer Res. 73:6745-6756.
    30. Liu,G. et al. (2012). ICAM-1–activated Src and eNOS Signaling Increase Endothelial Cell Surface PECAM-1 Adhesivity and Neutrophil Transmigration. Blood. 120:1942-1952.


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
CytoSelect™ 96-well Leukocyte-endothelium Adhesion Kit CBA-210 100 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
CBA-210
Product
CytoSelect™ 96-well Leukocyte-endothelium Adhesion Kit
Size
100 assays
Qty
Data Sheet
MSDS

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