고마바이오텍(주)

로그인이 필요합니다.

Category 전체보기
전기영동 > 단백질 전기영동 전기영동 > 헥산 전기영동
Cat. No.전체보기

검색 결과가 없습니다.

Product전체보기

검색 결과가 없습니다.

Brand전체보기

검색 결과가 없습니다.

제품 상세
세포 수축 측정 | Cell Contraction Assay Kit 이미지

세포 수축 측정 | Cell Contraction Assay Kit

CytoSelect™ Cell Contraction Assay Kit는 세포의 수축에 관여하는 cell contracting mediator를 스크리닝하고 cell contractivity를 측정합니다.

프린트 제품 문의

특징 


  • 상처 치유는 상피화, 결합조직 침착 및 수축의 세가지 과정으로 구성되며, 이중 수축과정은 근섬유아세포(myofibroblast)라고 불리는 특수화된 섬유아세포에 의해 매개됩니다. 3차원 collagen gel은 섬유아세포 수축연구에 널리 사용됩니다. 
  • Fibroblast의 collagen matrix 수축 신호 메커니즘에 관여하는 cell contracting mediator를 스크리닝하고 cell contractivity를 측정합니다. 



종류  

 

Cell Contraction Assays, Two-Step Attached Model (#CBA-201) 

 

  • Two-Step Collagen Contraction Model로,  stress matrix release 과정을 거칩니다. 
  • Cell에 collagen을 혼합하여 24-well plate에 넣어 1시간 동안 collagen polymerization을 유도하고 배양한 뒤, stress matrix 방출을 유도하여 collagen gel size의 변화를 시간별로 측정하고 image를 분석합니다. 
  • Kti의 구성
    • Collagen Solution
    • Neutralization Solution
    • 5X DMEM Medium
    • 5X PBS
    • 100X Cell Contraction Inhibitor

  

 

 cell-contraction분석 원리

 

Contraction inhibition by BDM

Contraction inhibition by BDM. 

0.5 x 10^6 COS-7 cells in 0.5 mL collagen gel lattice were cultured for two days. Before initiation of contraction, cells were pretreated with 10 mM BDM for 1 hr. 

The change of gel size (diameter) in millimeters was measured with a ruler at various times after release.

 

 

  

Cell Contraction Assays, Floating Matrix Model (#CBA-5020) 

  

  • Cell Contraction Plate를 이용하여 matrix releasing step을 생략함으로써 cell contraction 결과를 좀더 편리하고 빠르게 얻을 수 있습니다. 
  • Cell suspension에 cell contraction matrix를 혼합하여 24-well Cell Contraction Plate에 넣고 1시간 배양한 뒤 collagen polymerization이 일어나면 culture medium을 넣고 contraction을 모니터링하여 collagen gel size의 변화를 측정합니다. 
  • Kit의 구성
    • 24-Well Cell Contraction Plate (Floating Model)
    • Collagen Solution
    • Neutralization Solution
    • 5X DMEM Medium 
    • 5X PBS 
    • 100X Cell Contraction Inhibitor

  


The figure demonstrates typical contraction results using the Cell Contraction Assay. One should use the data below for reference only. 
This data should not be used to interpret actual results.

 

  



사용 논문 

 

CBA-201 　
    1. Lecce, L. et al. (2021). Histone deacetylase 9 promotes endothelial-mesenchymal transition and an unfavorable atherosclerotic plaque phenotype. J Clin Invest. 131(15):e131178. doi: 10.1172/JCI131178.
    2. Xiao, F. et al. (2021). Tumor-Suppressing STF cDNA 3 Overexpression Suppresses Renal Fibrosis by Alleviating Anoikis Resistance and Inhibiting the PI3K/Akt Pathway. Kidney Blood Press Res. doi: 10.1159/000517318.
    3. Yokota, M. et al. (2021). Staphylococcus aureus impairs dermal fibroblast functions with deleterious effects on wound healing. FASEB J. 35(7):e21695. doi: 10.1096/fj.201902836R.
    4. Morales, M.M. et al. (2021). O-GlcNAc modification of MYPT1 modulates lysophosphatidic acid (LPA)-induced cell contraction in fibroblasts. J Biol Chem. doi: 10.1016/j.jbc.2021.100800.
    5. Zhang, L. et al. (2021). Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy. Cell Death Dis. 12(5):470. doi: 10.1038/s41419-021-03750-4.
    6. Liu, P. et al. (2021). Fasudil Dichloroacetate Alleviates SU5416/Hypoxia-Induced Pulmonary Arterial Hypertension by Ameliorating Dysfunction of Pulmonary Arterial Smooth Muscle Cells. Drug Des Devel Ther. 15:1653-1666. doi: 10.2147/DDDT.S297500.
    7. Liu, Y. et al. (2021). 20-HETE-promoted cerebral blood flow autoregulation is associated with enhanced pericyte contractility. Prostaglandins Other Lipid Mediat. 154:106548. doi: 10.1016/j.prostaglandins.2021.106548.
    8. Kim, G.R. et al. (2021). MicroRNA-212-5p and its target PAFAH1B2 suppress vascular proliferation and contraction via the downregulation of RhoA. PLoS One. 16(3):e0249146. doi: 10.1371/journal.pone.0249146.
    9. Uemura, M. et al. (2021). Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts. Phys Ther Res. doi: 10.1298/ptr.E10064.
    10. Bai, L. et al. (2020). HDAC5 inhibition reduces angiotensin II-induced vascular contraction, hypertrophy, and oxidative stress in a mouse model. Biomed Pharmacother. 134:111162. doi: 10.1016/j.biopha.2020.111162.
    11. Maun, H.R. et al. (2020). Bivalent antibody pliers inhibit β-tryptase by an allosteric mechanism dependent on the IgG hinge. Nat Commun. 11(1):6435. doi: 10.1038/s41467-020-20143-x.
    12. Pedowitz, N.J. et al. (2020). MYPT1 O-GlcNAc modification regulates sphingosine-1-phosphate mediated contraction. Nat Chem Biol. doi: 10.1038/s41589-020-0640-8.
    13. Yan, D. et al. (2020). Mesothelial Cells Participate in Endometriosis Fibrogenesis Through Platelet-Induced Mesothelial-Mesenchymal Transition. J Clin Endocrinol Metab. doi: 10.1210/clinem/dgaa550.
    14. Ilg, M.M. et al. (2020). Phosphodiesterase Type 5 Inhibitors and Selective Estrogen Receptor Modulators Can Prevent But Not Reverse Myofibroblast Transformation in Peyronie's. J Sex Med. S1743-6095(20)30727-X. doi: 10.1016/j.jsxm.2020.06.022.
    15. Wójcik-Pszczoła, K. et al. (2020). A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling. Int J Mol Sci. 21(11):4008. doi: 10.3390/ijms21114008.
    16. Lin, H. et al. (2020). Anti-fibrotic effect of iguratimod on pulmonary fibrosis by inhibiting the fibroblast-to-myofibroblast transition. Adv Med Sci. 65(2):338-347. doi: 10.1016/j.advms.2020.05.006.
    17. Chow, A. et al. (2020). Dasatinib inhibits peripapillary scleral myofibroblast differentiation. Exp Eye Res. doi: 10.1016/j.exer.2020.107999.
    18. Wang, S. et al. (2020). Sex differences in the structure and function of rat middle cerebral arteries. Am J Physiol Heart Circ Physiol. doi: 10.1152/ajpheart.00722.2019.
    19. Lee, E. et al. (2020). New screening system using Twist1 promoter activity identifies dihydrorotenone as a potent drug targeting cancer-associated fibroblasts. Sci Rep. 10(1):7058. doi: 10.1038/s41598-020-63996-4.
    20. Guo, Y. et al. (2020). Accelerated cerebral vascular injury in diabetes is associated with vascular smooth muscle cell dysfunction. Geroscience. doi: 10.1007/s11357-020-00179-z.
    21. Chen, G. et al. (2020). Monocyte Chemotactic Protein-1 Regulates Proliferation and Contractility of Human Bladder Smooth Muscle Cells Under Hydrostatic Pressure. J Interferon Cytokine Res. doi: 10.1089/jir.2019.0186.
    22. Zhang, H. et al. (2020). Influence of dual-specificity protein phosphatase 5 on mechanical properties of rat cerebral and renal arterioles. Physiol Rep. 8(2):e14345. doi: 10.14814/phy2.14345.
    23. Jeong, H. et al. (2020). Sustained Nitric Oxide-Providing Small Molecule and Precise Release Behavior Study for Glaucoma Treatment. Mol Pharm. doi: 10.1021/acs.molpharmaceut.9b01137.
    24. Navarro, J. et al. (2019). In Vivo Evaluation of 3D Printed, Keratin-Based Hydrogels in a Porcine Thermal Burn Model. Tissue Eng Part A. doi: 10.1089/ten.TEA.2019.0181.
    25. Malhotra, R. et al. (2019). HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nat Genet. doi: 10.1038/s41588-019-0514-8.
    26. Shao, T. et al. (2019). Research on Function and Mechanisms of a Novel Small Molecule WG449E for Hypertrophic Scar. J Eur Acad Dermatol Venereol. doi: 10.1111/jdv.16028.
    27. Heffer, A.M. et al. (2019). The polyether ionophore salinomycin targets multiple cellular pathways to block proliferative vitreoretinopathy pathology. PLoS One. 14(9):e0222596. doi: 10.1371/journal.pone.0222596.
    28. Esteban-Vives, R. et al. (2019). Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies. Cell Transplant. doi: 10.1177/0963689718817524.
    29. Wen, J. et al. (2019). Inhibition of LPA1 Signaling Impedes Conversion of Human Tenon's Fibroblasts into Myofibroblasts Via Suppressing TGF-β/Smad2/3 Signaling. J Ocul Pharmacol Ther. 35(6):331-340. doi: 10.1089/jop.2018.0120.
    30. Canugovi, C. et al. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biology. doi:10.1016/j.redox.2019.101288.
CBA-5020 　
    1. Park, S.Y. et al. (2021). Nano-Graphene Oxide-Promoted Epithelial–Mesenchymal Transition of Human Retinal Pigment Epithelial Cells through Regulation of Phospholipase D Signaling. Nanomaterials. 11(10):2546. doi: 10.3390/nano11102546 (#CBA-5021).
    2. Peavey, M.C. et al. (2021). Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility. Proc Natl Acad Sci U S A. 118(11):e2011643118. doi: 10.1073/pnas.2011643118 (#CBA-5021).
    3. Ahn, S.H. et al. (2021). MicroRNA-139-5p Regulates Fibrotic Potentials via Modulation of Collagen Type 1 and Phosphorylated p38 MAPK in Uterine Leiomyoma. Yonsei Med J. 62(8):726-733. doi: 10.3349/ymj.2021.62.8.726 (#CBA-5020).
    4. Moreno, S.E. et al. (2021). Dehydrated Human Amniotic Membrane Inhibits Myofibroblast Contraction through Regulation of the TGF-β1/SMAD Pathway In Vitro. JID Innov. doi: 10.1016/j.xjidi.2021.100020 (#CBA-5020).
    5. Rao, P. et al. (2021). Promotion of β-catenin/Foxo signaling mediates epithelial repair in kidney injury. Am J Pathol. doi: 10.1016/j.ajpath.2021.03.005 (#CBA-5020).
    6. Pachera, E. et al. (2020). Long noncoding RNA H19X is a key mediator of TGFβ driven fibrosis. J Clin Invest. doi: 10.1172/JCI135439 (#CBA-5021).
    7. Knitlova, J. et al. (2020). Minoxidil decreases collagen I deposition and tissue-like contraction in clubfoot-derived cells: a way to improve conservative treatment of relapsed clubfoot? Connect Tissue Res. doi: 10.1080/03008207.2020.1816992 (#CBA-5020).
    8. Chen, G. et al. (2020). β-Adrenoceptor regulates contraction and inflammatory cytokine expression of human bladder smooth muscle cells via autophagy under pathological hydrostatic pressure. Neurourol Urodyn. doi: 10.1002/nau.24517 (#CBA-5020).
    9. Shimizu, H. et al. (2020). Caveolin-1 Promotes Cellular Senescence in Exchange for Blocking Subretinal Fibrosis in Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci. 61(11):21. doi: 10.1167/iovs.61.11.21 (#CBA-5021).
    10. Cao, X. et al. (2020). Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway. Ecotoxicol Environ Saf. 195:110491. doi: 10.1016/j.ecoenv.2020.110491 (#CBA-5021).
    11. Prosseda, P.P. et al. (2020). Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Sci Adv. doi: 10.1126/sciadv.aay8699 (#CBA-5020).
    12. Manetti, M. et al. (2019). Systemic Sclerosis Serum Steers the Differentiation of Adipose-Derived Stem Cells Toward Profibrotic Myofibroblasts: Pathophysiologic Implications. J Clin Med. 8(8). pii: E1256. doi: 10.3390/jcm8081256 (#CBA-5020).
    13. Duan, F.F. et al. (2019). P311 Promotes Lung Fibrosis via Stimulation of Transforming Growth Factor-β1, -β2, and -β3 Translation. Am J Respir Cell Mol Biol. 60(2):221-231. doi: 10.1165/rcmb.2018-0028OC (#CBA-5020).
    14. An, S. et al. (2019). Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies. Ocul Surf. pii: S1542-0124(19)30051-5. doi: 10.1016/j.jtos.2019.03.010 (#CBA-5020).
    15. Iwasaki, K. et al. (2019). Spontaneous differentiation of periodontal ligament stem cells into myofibroblast during ex vivo expansion. J Cell Physiol. doi: 10.1002/jcp.28639 (#CBA-5020).
    16. Li, S. et al. (2019). TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2019:1-17. doi:10.1155/2019/6408352 (#CBA-5021).

 

 

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Collagen-based Cell Contraction Assay CBA-201 24 assays CELL BIOLABS
CytoSelect™ 24-Well Cell Contraction Assay Kit (Floating Matrix Model) CBA-5020 24 assays CELL BIOLABS
CytoSelect™ 48-Well Cell Contraction Assay Kit (Floating Matrix Model) CBA-5021 48 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
CBA-201
Product
Collagen-based Cell Contraction Assay
Size
24 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-5020
Product
CytoSelect™ 24-Well Cell Contraction Assay Kit (Floating Matrix Model)
Size
24 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-5021
Product
CytoSelect™ 48-Well Cell Contraction Assay Kit (Floating Matrix Model)
Size
48 assays
Qty
Data Sheet
MSDS

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

웨비나/Video

TOP

제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

로그인

로그인