Cultrex Cell Invasion & Angiogenesis Assay Kit | 고마바이오텍

MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

HUVEC were harvested, counted and diluted in Endothelial Cell Base Media (which does not contain serum or angiogenic factors) or Endothelial Cell Growth Media, containing all supplements and growth factors necessary to support HUVEC growth. Trypsinized and harvested HUVEC were aliquoted at 104

 HUVEC per well onto gelled Cultrex RGF BME and thereafter cultured for four hours at 37 °C and 5 % CO2. Typical phase contrast images with HUVEC in Endothelial Cell Base Media without added angiogenic factors (A), in Endothelial Cell Growth Media with added factors (B) and in Endothelial Cell Growth Media (with angiogenic factors) in the presence of 5 μM sulforaphane (C) are shown at 10X magnification.