죽은 세포 염색 | Live-or-Dye Fixable Viability Staining Kit

시약 및 기기 상세

죽은 세포 염색 | Live-or-Dye Fixable Viability Staining Kit

Maker : BIOTIUM

Live-or-Dye는 유세포 분석 또는 현미경 검사시 죽은 세포의 단백질을 염색하므로, 살아있는 세포와 죽은 세포를 구별할 수 있습니다.

프린트 제품 문의

특징

Live-or-Dye™ Fixable Viability Stain은 유세포 분석(Flow Cytometry)이나 형광 현미경 관찰 시, 데이터의 정확도를 떨어뜨리는 '죽은 세포(Dead cells)'를 확실하게 골라내어 분석에서 제외(Gating out)할 수 있도록 도와주는 세포 생존성 염색 키트입니다. 세포막을 통과하지 못하는 아민 반응성 염료로, 세포막이 손상된 죽은 세포에 들어가 세포 내 단백질의 자유 아민과 공유결합하여 표지합니다. 살아있는 세포에서는 표면 단백질과 반응하지만, 표면 단백질의 양이 세포 내 단백질보다 훨씬 적기 때문에 죽은 세포에 비해 염색 강도가 낮으므로 세포의 상태를 구분할 수 있습니다.

고정/투과 처리 가능 (Fixable): 화학적 공유 결합을 하기 때문에, 세포를 고정(Fixation)하거나 투과화(Permeabilization) 처리를 해도 염색약이 빠져나오거나 다른 세포로 번지지 않고 안정적으로 유지됩니다. (항체 염색을 병행하는 다색 분석에 필수적)
다양한 스펙트럼 (18가지 색상): UV부터 적외선 영역까지 총 18가지의 다양한 파장(색상) 옵션을 제공하여, 기존에 구성해 둔 항체 패널과 겹치지 않게 유연하게 선택할 수 있습니다.
모든 세포에 적용 가능: 포유류 세포뿐만 아니라 박테리아(세균), 효모(Yeast) 등 다양한 생물종의 죽은 세포를 정확하게 구별해 냅니다.

Live-dead cell 식별 원리

세포막 투과성 기반: 이 염색약은 건강한 산 세포(Live cell)의 세포막은 통과하지 못합니다.
죽은 세포만 표적: 세포막이 손상되어 구멍이 난 죽은 세포 내부로만 침투합니다.
영구적 결합: 세포 내부로 들어간 염색약이 단백질의 아민기(Free amines)와 공유 결합(Covalent bond)을 형성하여 죽은 세포를 매우 밝고 강하게 염색합니다.

Panel 디자인에 용이한 다양한 컬러

주요 제품

1) Single color Live-or-Dye Kit

Live-or-Dye™ fixable dead cell dye & Anhydrous DMSO로 구성

Cat. No.	Viability Dye	Compatible lasers (nm)	Optimal detection channels	Notes
32018, 32018-T	Live-or-Dye™ 330/410	355, 375	BUV395
32002, 32002-T	Live-or-Dye™ 350/448	355, 375	DAPI
32014, 32014-T	Live-or-Dye™ 375/600	355, 375, 405	Spectral scan, BUV615, BV605	Developed for spectral cytometry
32003, 32003-T	Live-or-Dye™ 405/452	405	Pacific Blue®, BV421, BV450
32009, 32009-T	Live-or-Dye™ 405/545	405	AmCyan, BV510
32004, 32004-T	Live-or-Dye™ 488/515	488	FITC	Validated for microscopy
32012, 32012-T	Live-or-Dye™ 510/550	488, 532	Spectral scan	Developed for spectral cytometry
32005, 32005-T	Live-or-Dye™ 568/583	488, 532, 561	PE, PI	Validated for microscopy
32006, 32006-T	Live-or-Dye™ 594/614	488, 532, 561	PI, PE-CF®594, PE-Texas Red®	Validated for microscopy
32015, 32015-T	Live-or-Dye™ 615/740	633-640	Spectral scan, APC-Cy®7	Developed for spectral cytometry
32007, 32007-T	Live-or-Dye™ 640/662	633-640	APC	Validated for microscopy
32023, 32023-T	Live-or-Dye™ 650/720	633-640	APC-700
32013, 32013-T	Live-or-Dye™ 665/685	633-640	Spectral scan, AF700	Developed for spectral cytometry
32024, 32024-T	Live-or-Dye™ 700/745	633-640, 785	APC-Cy®7	Our best dye for the APC-Cy®7 channel
32008, 32008-T	Live-or-Dye™ 750/777	633-640, 785	APC-Cy®7, IR840
32011, 32011-T	Live-or-Dye™ 787/808	785, 808	APC-Cy®7, IR800
32021, 32021-T	Live-or-Dye™ 820/835	808	IR840
32022, 32022-T	Live-or-Dye™ 850/870	808	IR885

2) Live-or-Dye™ Sampler Kits

실험 장비와 목적에 맞춰 5가지 색상을 묶어놓은 샘플러 키트
5가지 Live-or-Dye™ fixable dead cell dyes & Anhydrous DMSO로 구성
Standard 키트 (#32016): 가장 일반적인 유세포 분석기 레이저 구성(UV, Violet, Blue, Yellow-Green, Red)에 최적화된 조합.
Spectral 키트 (#32017): 최근 많이 쓰이는 스펙트랄 유세포 분석기(예: Cytek® Aurora) 전용으로 검증된 조합. 패널의 빈틈을 채우기 좋은 파장들로 구성.

Product	Cat. No.	Included dyes: Ex/Em (Cat No.)	Application
Live-or-Dye™ Fixable Viability Sampler Kit, Standard	32016	• 350/488 (32002A) • 405/545 (32009A) • 488/515 (32004A) • 568/583 (32005A) • 640/662 (32007A)	Designed for use on most standard flow cytometry laser and filter configurations
Live-or-Dye™ Fixable Viability Sampler Kit, Spectral	32017	• 350/448 (32002A) • 375/600 (32014A) • 510/550 (32012A) • 615/740 (32015A) • 665/685 (32013A)	Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores

실험 데이터

Live-or-Dye™ 로 염색된 에탄올 처리된 HeLa 세포.

죽은 세포들은 Live-or-Dye™에 의해 밝게 염색되는 반면, 산 세포들은 최소한의 염색을 보여줍니다. 세포핵은 Hoechst(청색)로 염색되었습니다.

Live-or-Dye™ 568/583과 DAPI로 염색한 살아있는 대장균(E. coli)과 죽은 대장균의 혼합물.

Live-or-Dye™ 568/583은 죽은 세포를 붉은색으로 염색하고, DAPI는 모든 세포(의 핵산)를 푸른색으로 염색합니다.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
Live-or-Dye™ 330/410 Fixable Viability Staining Kit (200 assays)	32018	1 kit	BIOTIUM
Live-or-Dye™ 330/410 Fixable Viability Staining Kit, Trial Size (50 assays)	32018-T	1 kit	BIOTIUM
Live-or-Dye™ 375/600 Fixable Viability Staining Kit (200 assays)	32014	1 kit	BIOTIUM
Live-or-Dye™ 375/600 Fixable Viability Staining Kit, Trial Size (50 assays)	32014-T	1 KT	BIOTIUM
Live-or-Dye™ 405/452 Fixable Viability Staining Kit (200 assays)	32003	1 kit	BIOTIUM
Live-or-Dye™ 405/452 Fixable Viability Staining Kit, Trial Size (50 assays)	32003-T	1 kit	BIOTIUM
Live-or-Dye™ 405/545 Fixable Viability Staining Kit (200 assays)	32009	1 kit	BIOTIUM
Live-or-Dye™ 405/545 Fixable Viability Staining Kit, Trial Size (50 assays)	32009-T	1 kit	BIOTIUM
Live-or-Dye™ 488/515 Fixable Viability Staining Kit (200 assays)	32004	1 kit	BIOTIUM
Live-or-Dye™ 488/515 Fixable Viability Staining Kit, Trial Size (50 assays)	32004-T	1 kit	BIOTIUM
Live-or-Dye™ 510/550 Fixable Viability Staining Kit (200 assays)	32012	1 KT	BIOTIUM
Live-or-Dye™ 510/550 Fixable Viability Staining Kit, Trial Size (50 assays)	32012-T	1 KT	BIOTIUM
Live-or-Dye™ 568/583 Fixable Viability Staining Kit (200 assays)	32005	1 kit	BIOTIUM
Live-or-Dye™ 568/583 Fixable Viability Staining Kit, Trial Size (50 assays)	32005-T	1 kit	BIOTIUM
Live-or-Dye™ 594/614 Fixable Viability Staining Kit (200 assays)	32006	1 kit	BIOTIUM
Live-or-Dye™ 594/614 Fixable Viability Staining Kit, Trial Size (50 assays)	32006-T	1 kit	BIOTIUM
Live-or-Dye™ 615/740 Fixable Viability Staining Kit (200 assays)	32015	1 kit	BIOTIUM
Live-or-Dye™ 615/740 Fixable Viability Staining Kit, Trial Size (50 assays)	32015-T	1 KT	BIOTIUM
Live-or-Dye™ 640/662 Fixable Viability Staining Kit (200 assays)	32007	1 kit	BIOTIUM
Live-or-Dye™ 640/662 Fixable Viability Staining Kit, Trial Size (50 assays)	32007-T	1 kit	BIOTIUM
Live-or-Dye™ 650/720 Fixable Viability Staining Kits (200 assays)	32023	1 Kit	BIOTIUM
Live-or-Dye™ 650/720 Fixable Viability Staining Kits, Trial Size (50 assays)	32023-T	1 Kit	BIOTIUM
Live-or-Dye™ 665/685 Fixable Viability Staining Kit (200 assays)	32013	1 kit	BIOTIUM
Live-or-Dye™ 665/685 Fixable Viability Staining Kit, Trial Size (50 assays)	32013-T	1 KT	BIOTIUM
Live-or-Dye™ 700/745 Fixable Viability Staining Kit, Trial Size (50 assays)	32024-T	50 assays	BIOTIUM
Live-or-Dye™ 750/777 Fixable Viability Staining Kit (200 assays)	32008	1 kit	BIOTIUM
Live-or-Dye™ 750/777 Fixable Viability Staining Kit, Trial Size (50 assays)	32008-T	1 kit	BIOTIUM
Live-or-Dye™ 787/808 Fixable Viability Staining Kit (200 assays)	32011	1 KT	BIOTIUM
Live-or-Dye™ 787/808 Fixable Viability Staining Kit, Trial Size (50 assays)	32011-T	1 KT	BIOTIUM
Live-or-Dye™ 850/870 Fixable Viability Staining Kit, Trial Size (50 assays)	32022-T	50 assays	BIOTIUM
Live-or-Dye™ Fixable Viability Sampler Kit, Spectral	32017	1 KT	BIOTIUM
Live-or-Dye™ Fixable Viability Sampler Kit, Standard	32016	1 kit	BIOTIUM
Live-or-Dye™ UV/448 Fixable Viability Staining Kit (200 assays)	32002	1 kit	BIOTIUM
Live-or-Dye™ UV/448 Fixable Viability Staining Kit, Trial Size (50 assays)	32002-T	1 kit	BIOTIUM

전체보기

1
Maker	BIOTIUM
Cat.No.	32018
Product	Live-or-Dye™ 330/410 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet

2
Maker	BIOTIUM
Cat.No.	32018-T
Product	Live-or-Dye™ 330/410 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet

3
Maker	BIOTIUM
Cat.No.	32014
Product	Live-or-Dye™ 375/600 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

4
Maker	BIOTIUM
Cat.No.	32014-T
Product	Live-or-Dye™ 375/600 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 KT
Qty
Data Sheet
MSDS

5
Maker	BIOTIUM
Cat.No.	32003
Product	Live-or-Dye™ 405/452 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

6
Maker	BIOTIUM
Cat.No.	32003-T
Product	Live-or-Dye™ 405/452 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

7
Maker	BIOTIUM
Cat.No.	32009
Product	Live-or-Dye™ 405/545 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

8
Maker	BIOTIUM
Cat.No.	32009-T
Product	Live-or-Dye™ 405/545 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

9
Maker	BIOTIUM
Cat.No.	32004
Product	Live-or-Dye™ 488/515 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

10
Maker	BIOTIUM
Cat.No.	32004-T
Product	Live-or-Dye™ 488/515 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

11
Maker	BIOTIUM
Cat.No.	32012
Product	Live-or-Dye™ 510/550 Fixable Viability Staining Kit (200 assays)
Size	1 KT
Qty
Data Sheet
MSDS

12
Maker	BIOTIUM
Cat.No.	32012-T
Product	Live-or-Dye™ 510/550 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 KT
Qty
Data Sheet
MSDS

13
Maker	BIOTIUM
Cat.No.	32005
Product	Live-or-Dye™ 568/583 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

14
Maker	BIOTIUM
Cat.No.	32005-T
Product	Live-or-Dye™ 568/583 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

15
Maker	BIOTIUM
Cat.No.	32006
Product	Live-or-Dye™ 594/614 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

16
Maker	BIOTIUM
Cat.No.	32006-T
Product	Live-or-Dye™ 594/614 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

17
Maker	BIOTIUM
Cat.No.	32015
Product	Live-or-Dye™ 615/740 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

18
Maker	BIOTIUM
Cat.No.	32015-T
Product	Live-or-Dye™ 615/740 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 KT
Qty
Data Sheet
MSDS

19
Maker	BIOTIUM
Cat.No.	32007
Product	Live-or-Dye™ 640/662 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

20
Maker	BIOTIUM
Cat.No.	32007-T
Product	Live-or-Dye™ 640/662 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

21
Maker	BIOTIUM
Cat.No.	32023
Product	Live-or-Dye™ 650/720 Fixable Viability Staining Kits (200 assays)
Size	1 Kit
Qty
Data Sheet
MSDS

22
Maker	BIOTIUM
Cat.No.	32023-T
Product	Live-or-Dye™ 650/720 Fixable Viability Staining Kits, Trial Size (50 assays)
Size	1 Kit
Qty
Data Sheet
MSDS

23
Maker	BIOTIUM
Cat.No.	32013
Product	Live-or-Dye™ 665/685 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

24
Maker	BIOTIUM
Cat.No.	32013-T
Product	Live-or-Dye™ 665/685 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 KT
Qty
Data Sheet
MSDS

25
Maker	BIOTIUM
Cat.No.	32024-T
Product	Live-or-Dye™ 700/745 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	50 assays
Qty

26
Maker	BIOTIUM
Cat.No.	32008
Product	Live-or-Dye™ 750/777 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

27
Maker	BIOTIUM
Cat.No.	32008-T
Product	Live-or-Dye™ 750/777 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

28
Maker	BIOTIUM
Cat.No.	32011
Product	Live-or-Dye™ 787/808 Fixable Viability Staining Kit (200 assays)
Size	1 KT
Qty
Data Sheet
MSDS

29
Maker	BIOTIUM
Cat.No.	32011-T
Product	Live-or-Dye™ 787/808 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 KT
Qty
Data Sheet
MSDS

30
Maker	BIOTIUM
Cat.No.	32022-T
Product	Live-or-Dye™ 850/870 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	50 assays
Qty

31
Maker	BIOTIUM
Cat.No.	32017
Product	Live-or-Dye™ Fixable Viability Sampler Kit, Spectral
Size	1 KT
Qty
Data Sheet
MSDS

32
Maker	BIOTIUM
Cat.No.	32016
Product	Live-or-Dye™ Fixable Viability Sampler Kit, Standard
Size	1 kit
Qty
Data Sheet
MSDS

33
Maker	BIOTIUM
Cat.No.	32002
Product	Live-or-Dye™ UV/448 Fixable Viability Staining Kit (200 assays)
Size	1 kit
Qty
Data Sheet
MSDS

34
Maker	BIOTIUM
Cat.No.	32002-T
Product	Live-or-Dye™ UV/448 Fixable Viability Staining Kit, Trial Size (50 assays)
Size	1 kit
Qty
Data Sheet
MSDS

전체보기

자료

Live-or-Dye™ Fixable Viability Stains

[BIOTIUM]

Live/Dead cell을 구분하기 위한 Live-or-Dye kit을 소개합니다.
Cell Viability & Cell Death Brochure

[BIOTIUM]

세포의 생존과 사멸을 확인하기 위한 제품을 소개합니다.

죽은 세포 염색 | Live-or-Dye Fixable Viability Staining Kit | 고마바이오텍

죽은 세포 염색 | Live-or-Dye Fixable Viability Staining Kit

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