고마바이오텍(주)

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Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

Cellular Senescence Detection Kit (SA-β-Gal Staining) (#CBA-230)

 

  • 세포내 대사는 진행되지만 증식과 분열이 멈추는 노화상태 (Cellular Senescence)를 측정하는 Kit 입니다.
  • Senescence Associated ß-galactosidase (SA-β-Gal)에 의한 X-gal의 가수분해를 시각적으로 확인함으로써 노화상태의 cell을 모니터링 합니다.
  • blue로 염색된 노화된 세포를 현미경으로 관찰합니다.
  • Kit  구성 :
    - 100X Fixing Solution
    - Staining Solution A
    - Staining Solution B
    - Staining Solution C
    - Staining Solution D
    - X-gal Solution

 



96-Well Cellular Senescence Assay Kit (#CBA-231)


  • 형광 기질을 사용하여 SA-ß-Gal의 활성을 측정하여 세포 노화를 결정하는 Kit 입니다.
  • SA-ß-Galactosidase  활성 결정과 샘플의 정규화를 위해 cell lysate를 사용합니다. 
  • blue로 염색된 노화된 세포를 형광 plate reader로 측정합니다.
  • Kit  구성 :
    - 2X Cell Lysis Buffer
    - 2X Reaction Buffer
    - SA-ß-Gal Substrate (20X)
    - Stop Solution



SA-ß-Gal activity in Senescent Human Lung Fibroblast HFL-1 Cells. Normal HFL-1 cells with different passage numbers were lysed. Lysates were allowed to incubate with SA-ß-Gal Substrate for 1 hr at 37ºC. 

 

 

 

Quantitative Cellular Senescence Assay (#CBA-232) 

 
  • 무독성 형광 기질을 사용하여 SA-ß-Gal 활성을 측정합니다. 
  • 보다 정량적으로 측정하기 위해 유세포 분석을 이용합니다. 
  • 35mm dish에서 최대 10번의 assay를 진행할 수 있는 양의 시약이 제공됩니다.
  • blue로 염색된 노화된 세포를 flow cytometry나 epifluorescence microscope로 측정합니다. 
  • 구성 :
    - Cell Pretreatment Solution (1000X)
    - SA-ß-Gal Substrate (200X)

 

  

 

 

사용 논문 

 

CBA-230 　
    1. Jenuit, M. et al (2021). Establishment and Cryopreservation of Fibroblast Cell Line from a Sumatran Rhinoceros (Dicerorhinus sumatrensis). J. Sustain. Sci. Manag. 16(4):85-98. doi: 10.46754/jssm.2021.06.008.
    2. Ho, D.H. et al. (2021). LRRK2 Kinase Inhibitor Rejuvenates Oxidative Stress-Induced Cellular Senescence in Neuronal Cells. Oxid Med Cell Longev. doi: 10.1155/2021/9969842.
    3. Cui, Z. et al. (2021). Effect of the traditional Chinese medicine Pinggan-Qianyang decoction on SIRT1-PTEN signaling in vascular aging in spontaneously hypertensive rats. Hypertens Res. doi: 10.1038/s41440-021-00682-6.
    4. Zhang, Y. et al. (2021). Salidroside Ameliorates Vascular Endothelial Cell Senescence through Downregulation of KLF4. J Biosci Med (Irvine). 9(2):21-32. doi: 10.4236/jbm.2021.92003.
    5. Kim, S.N. et al. (2020). Culturing at Low Cell Density Delays Cellular Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells in Long-Term Cultures. Int J Stem Cells. doi: 10.15283/ijsc20078.
    6. Baek, A.R. et al. (2020). Spermidine attenuates bleomycin-induced lung fibrosis by inducing autophagy and inhibiting endoplasmic reticulum stress (ERS)-induced cell death in mice. Exp Mol Med. doi: 10.1038/s12276-020-00545-z.
    7. Hwang, S.G. et al. (2020). Cold atmospheric plasma prevents wrinkle formation via an anti-aging process. Plasma Med. doi: 10.1615/PlasmaMed.2020034810.
    8. Madonna, R. et al. (2020). Empagliflozin reduces the senescence of cardiac stromal cells and improves cardiac function in a murine model of diabetes. J Cell Mol Med. doi: 10.1111/jcmm.15699.
    9. Takei, Y. et al. (2020). Quality assessment tests for tumorigenicity of human iPS cell-derived cartilage. Sci Rep. 10(1):12794. doi: 10.1038/s41598-020-69641-4.
    10. Azmi, S.M. et al. (2020). Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration. Regen Med. doi: 10.2217/rme-2019-0103.
    11. Yamazaki, H. et al. (2020). Ribosome binding protein GCN1 regulates the cell cycle and cell proliferation and is essential for the embryonic development of mice. PLoS Genet. 16(4):e1008693. doi: 10.1371/journal.pgen.1008693.
    12. Sugimoto, H. et al. (2020). Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions. Biomed Res. 41(1):43-51. doi: 10.2220/biomedres.41.43.
    13. Sogawa, K. et al. (2020). Effects of continuous exposure to low concentration of ClO2 gas on the growth, viability, and maintenance of undifferentiated MSCs in long-term cultures. Regen Ther. 14:184–190. doi: 10.1016/j.reth.2019.12.007.
    14. Tan, J. et al. (2019). An R-loop-initiated CSB-RAD52-POLD3 pathway suppresses ROS-induced telomeric DNA breaks. Nucleic Acids Res. pii: gkz1114. doi: 10.1093/nar/gkz1114.
    15. O'Hara, S.P. et al. (2019). The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1. J Biol Chem. pii: jbc.RA119.010176. doi: 10.1074/jbc.RA119.010176.
    16. Cilibrasi, C.  et al. (2019). A Ploidy Increase Promotes Sensitivity of Glioma Stem Cells to Aurora Kinases Inhibition. Journal of Oncology. doi: 10.1155/2019/9014045.
    17. Canugovi, C. et al. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biology. doi:10.1016/j.redox.2019.10128
    18. Hong, S.H. et al. (2019). Stem cell passage affects directional migration of stem cells in electrotaxis. Stem Cell Research. 101475. doi:10.1016/j.scr.2019.101475.
    19. Kim, Y.Y. et al. (2019). Modulatory Effects of Single and Complex Vitamins on the In Vitro Growth of Murine Ovarian Follicles. Tissue Engineering and Regenerative Medicine. doi:10.1007/s13770-019-00188-w.
    20. Fang, B. et al. (2019). The effects of mechanical stretch on the biological characteristics of human adipose-derived stem cells. J Cell Mol Med. doi: 10.1111/jcmm.14314.
    21. Tominaga, T. et al. (2019). Senescence-associated-β-galactosidase staining following traumatic brain injury in the mouse cerebrum. PLoS One. 14(3):e0213673. doi: 10.1371/journal.pone.0213673.
    22. Cho, S.Y. et al. (2019). Oxytocin Alleviates Cellular Senescence through Oxytocin Receptor-Mediated ERK/Nrf2 Signalling. Br J Dermatol. doi: 10.1111/bjd.17824.
    23. Odagiri, N. et al. (2018). Involvement of ERK1/2 activation in the gene expression of senescence-associated secretory factors in human hepatic stellate cells. Mol Cell Biochem. doi: 10.1007/s11010-018-3466-x.
    24. Park, M.J. et al. (2018). Dexamethasone Induces a Specific Form of Ramified Dysfunctional Microglia. Mol Neurobiol. 10.1007/s12035-018-1156-z.
    25. Chen, B. et al. (2018). Overexpression of Klotho Inhibits HELF Fibroblasts SASP-related Protumoral Effects on Non-small Cell Lung Cancer Cells. J Cancer. 9(7):1248-1258. doi: 10.7150/jca.23967.
    26. Chijimatsu, R. et al. (2017). Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair. Stem Cells Int. 2017:1960965. doi: 10.1155/2017/1960965.
    27. Lines, K.E. et al. (2017). Epigenetic pathway inhibitors represent potential drugs for treating pancreatic and bronchial neuroendocrine tumors. Oncogenesis. 6(5):e332. doi: 10.1038/oncsis.2017.30.
    28. Murai, S. et al. (2017). Inhibition of malic enzyme 1 disrupts cellular metabolism and leads to vulnerability in cancer cells in glucose-restricted conditions. Oncogenesis. 6:e329.
    29. O'Hara, S.P. et al. (2017). ETS Proto-Oncogene 1 Transcriptionally Up-regulates the Cholangiocyte Senescence-associated Protein Cyclin Dependent Kinase Inhibitor 2A. J Biol Chem. doi: 10.1074/jbc.M117.777409. 
    30. Zhang, X. et al. (2016). Cryptosporidium parvum infection attenuates the ex vivo propagation of murine intestinal enteroids. PHY2 4:e13060.
CBA-231 　
    1. Deng, Z. et al. (2021). Biofunction of Polydopamine Coating in Stem Cell Culture. ACS Appl Mater Interfaces. doi: 10.1021/acsami.0c22565.
    2. Lee, Y.N. et al. (2021). Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells. Sci Rep. 11(1):13449. doi: 10.1038/s41598-021-92754-3.
    3. Hirata, Y. et al. (2021). Advanced maternal age induces fetal growth restriction through decreased placental inflammatory cytokine expression and immune cell accumulation in mice. J Reprod Dev. doi: 10.1262/jrd.2021-034.
    4. Mehdi, S.J. et al. (2021). Normal and cancer fibroblasts differentially regulate TWIST1, TOX and cytokine gene expression in cutaneous T-cell lymphoma. BMC Cancer. 21(1):492. doi: 10.1186/s12885-021-08142-7.
    5. Bourdon, B. et al. (2021). Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes. Int. J. Mol. Sci. 22(7):3693. doi: 10.3390/ijms22073693.
    6. Kong, C.S. et al. (2021). Embryo biosensing by uterine natural killer cells determines endometrial fate decisions at implantation. FASEB J. 35(4):e21336. doi: 10.1096/fj.202002217R.
    7. Baxley, R.M. et al. (2021). Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nat Commun. 12(1):1626. doi: 10.1038/s41467-021-21878-x.
    8. Yamaguchi, S. et al. (2021). Characterization of an active LINE-1 in the naked mole-rat genome. Sci Rep. 11(1):5725. doi: 10.1038/s41598-021-84962-8.
    9. Bourdon, B. et al. (2021). Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids. Int J Mol Sci. 22(2):E580. doi: 10.3390/ijms22020580.
    10. Jiang, Y. et al. (2021). Histone H3K27 methyltransferase EZH2 and demethylase JMJD3 regulate hepatic stellate cells activation and liver fibrosis. Theranostics. 11(1):361-378. doi: 10.7150/thno.46360.
    11. Mogilenko, D.A. et al. (2020). Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK+ CD8+ T Cells as Conserved Hallmark of Inflammaging. Immunity. doi: 10.1016/j.immuni.2020.11.005.
    12. Setoguchi, Y. et al. (2020). Functional assessment of retinal pigment epithelium cell transplants with various degrees of pigmentation for age-related macular degeneration. Kawasaki Medical Journal. 46:49-58. doi: 10.11482/KMJ-E202046049.
    13. Rostami, A. et al. (2020). Senescence, Necrosis, and Apoptosis Govern Circulating Cell-free DNA Release Kinetics. Cell Rep. 31(13):107830. doi: 10.1016/j.celrep.2020.107830.
    14. Pacifici, F. et al. (2020). Prdx6 Plays a Main Role in the Crosstalk Between Aging and Metabolic Sarcopenia. Antioxidants (Basel). 9(4). pii: E329. doi: 10.3390/antiox9040329.
    15. Lin, X. et al. (2020). Excessive oxidative stress in cumulus granulosa cells induced cell senescence contributes to endometriosis-associated infertility. Redox Biol. 30:101431. doi: 10.1016/j.redox.2020.101431.
    16. Ohigashi, T. et al. (2019). Protective effect of phosphatidylcholine on lysophosphatidylcholine-induced cellular senescence in cholangiocyte. J Hepatobiliary Pancreat Sci. doi: 10.1002/jhbp.684.
    17. Takagi, H. et al. (2019). Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells. Anticancer Res. 39(9):4811-4816. doi: 10.21873/anticanres.13666.
    18. Tencerova, M. et al. (2019). Obesity-Associated Hypermetabolism and Accelerated Senescence of Bone Marrow Stromal Stem Cells Suggest a Potential Mechanism for Bone Fragility. Cell Rep. 27(7):2050-2062.e6. doi: 10.1016/j.celrep.2019.04.066.
    19. Morsczeck, C. et al. (2019). Short telomeres correlate with a strong induction of cellular senescence in human dental follicle cells. BMC Mol Cell Biol. 20(1):5. doi: 10.1186/s12860-019-0185-4.
    20. Cho, S.Y. et al. (2019). Oxytocin Alleviates Cellular Senescence through Oxytocin Receptor-Mediated ERK/Nrf2 Signalling. Br J Dermatol. doi: 10.1111/bjd.17824.
    21. Cao, J. et al. (2019). Combining CDK4/6 inhibition with taxanes enhances anti-tumor efficacy by sustained impairment of pRB-E2F pathways in squamous cell lung cancer. Oncogene. doi: 10.1038/s41388-019-0708-7.
    22. Mehdi, S.J. et al. (2019). Mesenchymal stem cells gene signature in high-risk myeloma bone marrow linked to suppression of distinct IGFBP2-expressing small adipocytes. Br J Haematol. 184(4):578-593. doi: 10.1111/bjh.15669.
    23. Velusami, C.C. et al. (2018). Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action. Inflammopharmacology. 26(5):1233-1243. doi: 10.1007/s10787-017-0433-1.
    24. Perla, V. et al. (2018). Effect of ghost pepper on cell proliferation, apoptosis, senescence and global proteomic profile in human renal adenocarcinoma cells. PLoS One. 13(10):e0206183. doi: 10.1371/journal.pone.0206183.
    25. Rana, K. et al. (2018). Bone marrow neutrophil aging in sickle cell disease mice is associated with impaired osteoblast functions. Biochem Biophys Rep. 16:110-114. doi: 10.1016/j.bbrep.2018.10.009.
    26. Oja, S. et al. (2018). Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures. Stem Cell Res Ther. 9(1):6. doi: 10.1186/s13287-017-0740-x.
    27. Xu, R. et al. (2017). Tumor suppressor p53 links ceramide metabolism to DNA damage response through alkaline ceramidase 2. Cell Death Differ. 25(5):841-856. doi: 10.1038/s41418-017-0018-y.
    28. Jeong, J. H. et al. (2017). Inhibitory effect of vitamin C on intrinsic aging in human dermal fibroblasts and hairless mice. Food Sci Biotechnol. 27(2):555-564. doi: 10.1007/s10068-017-0252-6.
    29. Empl, M.T. et al. (2018). Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies. Mol Nutr Food Res. 62(2). doi: 10.1002/mnfr.201700450.
    30. Tofiño-Vian, M. et al. (2017). Extracellular Vesicles from Adipose-Derived Mesenchymal Stem Cells Downregulate Senescence Features in Osteoarthritic Osteoblasts. Oxid Med Cell Longev. 2017:7197598. doi: 10.1155/2017/7197598.
CBA-232 　
    1. Cho, E. et al. (2021). Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease. Genes. 12(7):1066. doi: 10.3390/genes12071066.
    2. Nishizawa, H. et al. (2021). Lipid peroxidation and the subsequent cell death transmitting from ferroptotic cells to neighboring cells. Cell Death Dis. 12(4):332. doi: 10.1038/s41419-021-03613-y.
    3. Martini, H. et al. (2021). Selective Cardiomyocyte Oxidative Stress Leads to Bystander Senescence of Cardiac Stromal Cells. Int. J. Mol. Sci. 22(5):2245. doi: 10.3390/ijms22052245.
    4. Rothmiller, S. et al. (2021). Chronic senescent human mesenchymal stem cells as possible contributor to the wound healing disorder after exposure to the alkylating agent sulfur mustard. Arch Toxicol. doi: 10.1007/s00204-020-02946-5.
    5. Kim, S.N. et al. (2020). Culturing at Low Cell Density Delays Cellular Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells in Long-Term Cultures. Int J Stem Cells. doi: 10.15283/ijsc20078.
    6. Jun, E.S. et al. (2020). Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3. Mar Drugs. 18(9):E433. doi: 10.3390/md18090433.
    7. van den Berg, J. et al. (2020). Development of transient radioresistance during fractionated irradiation in vitro. Radiother Oncol. doi: 10.1016/j.radonc.2020.04.014.
    8. Fessler, J. et al. (2020). Lymphopenia in primary Sjögren's syndrome is associated with premature aging of naïve CD4+ T cells. Rheumatology (Oxford). pii: keaa105. doi: 10.1093/rheumatology/keaa105.
    9. di Martino, S. et al. (2018). HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome. Oncogene. 37(10):1369-1385. doi: 10.1038/s41388-017-0044-8.
    10. Nojima, T. et al. (2018). Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence. Mol Cell. 72(6):970-984.e7. doi: 10.1016/j.molcel.2018.10.011.
    11. Watanabe, J. et al. (2018). Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress. Biomaterials. 185:25-38. doi: 10.1016/j.biomaterials.2018.08.055.
    12. Nagane, M. et al. (2018). Ataxia-Telangiectasia Mutated (ATM) Kinase Regulates eNOS Expression and Modulates Radiosensitivity in Endothelial Cells Exposed to Ionizing Radiation. Radiat Res. 189(5):519-528. doi: 10.1667/RR14781.1.
    13. Poulos, M.G. et al. (2017). Endothelial transplantation rejuvenates aged hematopoietic stem cell function. J Clin Invest. 127(11):4163-4178. doi: 10.1172/JCI93940.
    14. Won, Y.H. et al. (2016). Elucidation of relevant neuroinflammation mechanisms using gene expression profiling in patients with amyotrophic lateral sclerosis. PLoS One 11:e0165290.
    15. Chae, S. Y. et al. (2016). Gardenia jasminoides extract-capped gold nanoparticles reverse hydrogen peroxide-induced premature senescence. J. Photochem Photobiol B.  doi:10.1016/j.jphotobiol.2016.09.033.
    16. Hu, W. et al. (2015). Mechanistic investigation of bone marrow suppression associated with palbociclib and its differentiation from cytotoxic chemotherapies. Clin Cancer Res. doi:10.1158/1078-0432.CCR-15-1421.
    17. Kim, J. et al. (2014) p53 Induces Skin Aging by Depleting Blimp1+ Sebaceous Gland Cells. Cell Death Dis. 5:e1141
    18. Grasso, D. et al. (2014). Genetic Inactivation of the Pancreatitis-Inducible Gene Nupr1 Impairs PanIN Formation by Modulating Kras(G12D)-Induced Senescence. Cell Death Differ. 21:1633-1641.
    19. Landowski, T. H. et al. (2014).  Targeting Integrin α6 Stimulates Curative-Type Bone Metastasis Lesions in a Xenograft Model. Mol Cancer Ther. 13:1558-1566.

 

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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