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6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

Cellular Senescence Detection Kit (SA-β-Gal Staining) (#CBA-230)


  • 세포내 대사는 진행되지만 증식과 분열이 멈추는 노화상태 (Cellular Senescence)를 측정하는 Kit 입니다.
  • Senescence Associated ß-galactosidase (SA-β-Gal)에 의한 X-gal의 가수분해를 시각적으로 확인함으로써 노화상태의 cell을 모니터링 합니다.
  • blue로 염색된 노화된 세포를 현미경으로 관찰합니다.
  • Kit  구성 :
    - 100X Fixing Solution
    - Staining Solution A
    - Staining Solution B
    - Staining Solution C
    - Staining Solution D
    - X-gal Solution


96-Well Cellular Senescence Assay Kit (#CBA-231)

  • 형광 기질을 사용하여 SA-ß-Gal의 활성을 측정하여 세포 노화를 결정하는 Kit 입니다.
  • SA-ß-Galactosidase  활성 결정과 샘플의 정규화를 위해 cell lysate를 사용합니다. 
  • blue로 염색된 노화된 세포를 형광 plate reader로 측정합니다.
  • Kit  구성 :
    - 2X Cell Lysis Buffer
    - 2X Reaction Buffer
    - SA-ß-Gal Substrate (20X)
    - Stop Solution

SA-ß-Gal activity in Senescent Human Lung Fibroblast HFL-1 Cells. Normal HFL-1 cells with different passage numbers were lysed. Lysates were allowed to incubate with SA-ß-Gal Substrate for 1 hr at 37ºC. 




Quantitative Cellular Senescence Assay (#CBA-232) 

  • 무독성 형광 기질을 사용하여 SA-ß-Gal 활성을 측정합니다. 
  • 보다 정량적으로 측정하기 위해 유세포 분석을 이용합니다. 
  • 35mm dish에서 최대 10번의 assay를 진행할 수 있는 양의 시약이 제공됩니다.
  • blue로 염색된 노화된 세포를 flow cytometry나 epifluorescence microscope로 측정합니다. 
  • 구성 :
    - Cell Pretreatment Solution (1000X)
    - SA-ß-Gal Substrate (200X)





사용 논문 


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    2. Ho, D.H. et al. (2021). LRRK2 Kinase Inhibitor Rejuvenates Oxidative Stress-Induced Cellular Senescence in Neuronal Cells. Oxid Med Cell Longev. doi: 10.1155/2021/9969842.
    3. Cui, Z. et al. (2021). Effect of the traditional Chinese medicine Pinggan-Qianyang decoction on SIRT1-PTEN signaling in vascular aging in spontaneously hypertensive rats. Hypertens Res. doi: 10.1038/s41440-021-00682-6.
    4. Zhang, Y. et al. (2021). Salidroside Ameliorates Vascular Endothelial Cell Senescence through Downregulation of KLF4. J Biosci Med (Irvine)9(2):21-32. doi: 10.4236/jbm.2021.92003.
    5. Kim, S.N. et al. (2020). Culturing at Low Cell Density Delays Cellular Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells in Long-Term Cultures. Int J Stem Cells. doi: 10.15283/ijsc20078.
    6. Baek, A.R. et al. (2020). Spermidine attenuates bleomycin-induced lung fibrosis by inducing autophagy and inhibiting endoplasmic reticulum stress (ERS)-induced cell death in mice. Exp Mol Med. doi: 10.1038/s12276-020-00545-z.
    7. Hwang, S.G. et al. (2020). Cold atmospheric plasma prevents wrinkle formation via an anti-aging process. Plasma Med. doi: 10.1615/PlasmaMed.2020034810.
    8. Madonna, R. et al. (2020). Empagliflozin reduces the senescence of cardiac stromal cells and improves cardiac function in a murine model of diabetes. J Cell Mol Med. doi: 10.1111/jcmm.15699.
    9. Takei, Y. et al. (2020). Quality assessment tests for tumorigenicity of human iPS cell-derived cartilage. Sci Rep10(1):12794. doi: 10.1038/s41598-020-69641-4.
    10. Azmi, S.M. et al. (2020). Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration. Regen Med. doi: 10.2217/rme-2019-0103.
    11. Yamazaki, H. et al. (2020). Ribosome binding protein GCN1 regulates the cell cycle and cell proliferation and is essential for the embryonic development of mice. PLoS Genet16(4):e1008693. doi: 10.1371/journal.pgen.1008693.
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    13. Sogawa, K. et al. (2020). Effects of continuous exposure to low concentration of ClO2 gas on the growth, viability, and maintenance of undifferentiated MSCs in long-term cultures. Regen Ther14:184–190. doi: 10.1016/j.reth.2019.12.007.
    14. Tan, J. et al. (2019). An R-loop-initiated CSB-RAD52-POLD3 pathway suppresses ROS-induced telomeric DNA breaks. Nucleic Acids Res. pii: gkz1114. doi: 10.1093/nar/gkz1114.
    15. O'Hara, S.P. et al. (2019). The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1. J Biol Chem. pii: jbc.RA119.010176. doi: 10.1074/jbc.RA119.010176.
    16. Cilibrasi, C.  et al. (2019). A Ploidy Increase Promotes Sensitivity of Glioma Stem Cells to Aurora Kinases Inhibition. Journal of Oncology. doi: 10.1155/2019/9014045.
    17. Canugovi, C. et al. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biology. doi:10.1016/j.redox.2019.10128
    18. Hong, S.H. et al. (2019). Stem cell passage affects directional migration of stem cells in electrotaxis. Stem Cell Research. 101475. doi:10.1016/j.scr.2019.101475.
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    22. Cho, S.Y. et al. (2019). Oxytocin Alleviates Cellular Senescence through Oxytocin Receptor-Mediated ERK/Nrf2 Signalling. Br J Dermatol. doi: 10.1111/bjd.17824.
    23. Odagiri, N. et al. (2018). Involvement of ERK1/2 activation in the gene expression of senescence-associated secretory factors in human hepatic stellate cells. Mol Cell Biochem. doi: 10.1007/s11010-018-3466-x.
    24. Park, M.J. et al. (2018). Dexamethasone Induces a Specific Form of Ramified Dysfunctional Microglia. Mol Neurobiol. 10.1007/s12035-018-1156-z.
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    26. Chijimatsu, R. et al. (2017). Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair. Stem Cells Int2017:1960965. doi: 10.1155/2017/1960965.
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    28. Murai, S. et al. (2017). Inhibition of malic enzyme 1 disrupts cellular metabolism and leads to vulnerability in cancer cells in glucose-restricted conditions. Oncogenesis6:e329.
    29. O'Hara, S.P. et al. (2017). ETS Proto-Oncogene 1 Transcriptionally Up-regulates the Cholangiocyte Senescence-associated Protein Cyclin Dependent Kinase Inhibitor 2A. J Biol Chem. doi: 10.1074/jbc.M117.777409. 
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