IMAC 단백질 정제용 Proteus Ni Advance Spin Column Kit

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시약 및 기기 상세

Proteus Ni Advance Spin Column Kit는 poly-Histidine tagged 재조합 단백질의 친화성 정제를 위해 사용됩니다.

프린트 제품 문의

특징

Proteus Ni Advance Spin Column Kit는 신속한 one-step batch binding purification을 위해 디자인된 제품으로, prep용 정제와 오염물질 제거에 유용합니다.
Spin column과 함께 loose resin이 제공되어 poly-histidine tagged recombinant protein을 정제할 때 이용됩니다.
chelating coupled ligand를 이용하여 Ni2+ 이온을 6% highly cross-linked agarose matrix에 로딩합니다.
Ni2+ 이온 누출을 최소화하고, 단백질 결합력이 매우 높습니다.
Fastback Ni Advance resin은 EDTA(최대 20mM), DTT(최대 20mM) 환경에서 사용할 수 있습니다.
Ni2+SO4와의 recharging procedure가 간단합니다.
다른 결합 친화성을 위해 호환되는 금속 이온(Co2+, Cu2+, Zn2+, Fe3+, Al3+)으로 충전할 수 있습니다.
Wash와 regeneration protocol이 간단합니다.

Resin Specification

구성품

1) Ni Advance Mini Kits

Proteus Kit code	NiAdv2	NiAdv 12	NiAdv 48
Vials containing 0.25 ml Fastback Ni-Advance resin	2	12	48
Proteus spin column barrels with clear spin push cap (20ml capacity in a swing bucket rotor)	2	12	48
Yellow batch incubation screw caps	2	12	48
50 ml centrifuge tubes	2	12

2) Ni Advance Micro Kits

Proteus Kit code	NiAdv2Micro	NiAdv24Micro	NiAdv100Micro
Fastback Ni-Advance resin	1 x 0.2 ml	1 x 2.4 ml	1 x 10 ml
Proteus spin column (600 µl capacity in a fixed angle rotor)	2	24	100
2.2 ml centrifuge tubes	4	48	100

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
Proteus NiADV (Micro) 100 column kit (includes 10.0 ml NiADV resin vial)	GEN-NiADV-100Micro	100column	PROTEIN ARK
Proteus NiADV (Micro) 2 column kit (includes 0.2 ml NiADV resin vial)	GEN-NiADV-2Micro	2column	PROTEIN ARK
Proteus NiADV (Micro) 24 column kit (includes 2.4 ml NiADV resin vial)	GEN-NiADV-24Micro	24column	PROTEIN ARK
Proteus NiADV (Mini) 12 column kit (includes 12 x 0.25 ml NiADV resin vials)	GEN-NiADV-12M	12column	PROTEIN ARK
Proteus NiADV (Mini) 2 column kit (includes 2 x 0.25 ml NiADV resin vials)	GEN-NiADV-2M	2column	PROTEIN ARK
Proteus NiADV (Mini) 48 column kit (includes 48 x 0.25 ml NiADV Resin vials)	GEN-NiADV-48M	48column	PROTEIN ARK

1
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-100Micro
Product	Proteus NiADV (Micro) 100 column kit (includes 10.0 ml NiADV resin vial)
Size	100column
Qty

2
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-2Micro
Product	Proteus NiADV (Micro) 2 column kit (includes 0.2 ml NiADV resin vial)
Size	2column
Qty

3
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-24Micro
Product	Proteus NiADV (Micro) 24 column kit (includes 2.4 ml NiADV resin vial)
Size	24column
Qty

4
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-12M
Product	Proteus NiADV (Mini) 12 column kit (includes 12 x 0.25 ml NiADV resin vials)
Size	12column
Qty

5
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-2M
Product	Proteus NiADV (Mini) 2 column kit (includes 2 x 0.25 ml NiADV resin vials)
Size	2column
Qty

6
Maker	PROTEIN ARK
Cat.No.	GEN-NiADV-48M
Product	Proteus NiADV (Mini) 48 column kit (includes 48 x 0.25 ml NiADV Resin vials)
Size	48column
Qty