엔도톡신 제거 | Endotoxin Removal Spin Column Kit | 고마바이오텍

적용

Performance

Figure 1: TheProteus NoEndo™ HC spin columns effectively remove endotoxin from BSA and rabbit IgG samples (1mg/ml) spiked with E.coli lysate. The Proteus NoEndo™ HC spin columns were pre-equilibrated with PBS (pH 7.5) and 20 ml protein samples were loaded and centrifuged at 100 g for 30 min. The flow throughs were loaded on to second columns and centrifuged using the same conditions.

Endotoxin data was generated using the kinetic chromogenic LAL assay (Charles River Endosafe plate reader). Typically, a 4 log reduction in endotoxin was observed. The protein recoveries were determined separately with the Proteus NoEndo™ HC spin columns.

Figure 2: The Proteus NoEndo™ M spin columns effectively removes endotoxin from BSA and rabbit IgG samples (1mg/ml) spiked with E.coli lysate. The Proteus NoEndo™ M spin columns were loaded with 0.25 ml NoEndo™ resin and washed at 500 g for 5 min to remove the resin storage buffer. The column resins were then washed with 15 ml equilibration buffer twice. 20 ml protein sample was batch incubated with the washed resin for up to 3 hours on a standard tube roller. The columns were centrifuged at 700 g for 10 min. Endotoxin data was generated using the kinetic chromogenic LAL assay (Charles River Endosafe plate reader). Typically, 3 log reductions in endotoxin were observed.