고마바이오텍(주)

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고정화 금속 친화 크로마토그래피 IMAC Resins 이미지

고정화 금속 친화 크로마토그래피 IMAC Resins

고정화 금속 친화 크로마토그래피(IMAC)는 폴리펩타이드, 단백질 및 DNA와 같은 거대 분자에 대한 선택적 샘플 정제 방법입니다.

프린트 제품 문의

특징

 

  • Immobilized metal affinity chromatography (IMAC) 정제는 chelating 그룹(예: Nitrilotriacetic Acid (NTA) or Iminodiacetic Acid (IDA))을 안정한 크로마토그래피 수지(예: agarose)에 coupling하여 금속 이온 결합을 활용합니다. 
  • Ni2+, Co2+, Cu2+ 또는 Zn2+와 같은 전이 금속 이온을 킬레이트 그룹에 적재하고 고정하여 높은 친화도 결합을 가능하게 합니다.
  • 니켈과 코발트는 His-태그에 대해 가장 높은 친화도(Ni2+>Co2+)와 가장 높은 특이성(Co2+>Ni2+)을 부여하기 때문에 가장 널리 사용되는 금속 이온입니다.
  • Super 타입 resin의 bead 평균 사이즈는 40um, Fastback 타입은 90um 입니다. 

 

 

종류  

 

　 Super Ni-NTA Affinity HP Resin Super Co-NTA Affinity HP Resin Super Zn-NTA Resin Fastback Zn-NTA Resin Fastback Co IDA Resin Fastback Ni IDA Resin
Specificity His-Tag His-Tag 　polyhistidine tag polyhistidine tag His-Tag His-Tag
Matrix 7.5% cross-linked Agarose 7.5% cross-linked Agarose 6% cross-linked Agarose 6% cross-linked Agarose 6% cross-linked Agarose 6% cross-linked Agarose
Binding capacity 70 mg/ml 30 mg/ml 20 mg / ml resin 20 mg / ml resin  10 mg/ml 10 mg/ml
Bead size range 32-60 µm 32-60 µm 20 – 50 µm  50 – 150 µm 45-165 µm 45-165 µm
Maximum pressure 72 psi 72 psi 　 　 42 psi 42 psi
pH Stability pH 2-14 pH 2-14 2-14 (<2 h), 3-12 (up to one week)  2-14 (<2 h), 3-12 (up to one week)  pH 2-12 pH 2-12

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Fastback Co IMAC Resin (10 ml) Fastback-Co-10 10 ml PROTEIN ARK
Fastback Co IMAC Resin (100 ml) Fastback-Co-100 100 ml PROTEIN ARK
Fastback Co IMAC Resin (25 ml) Fastback-Co-25 25 ml PROTEIN ARK
Fastback IDA Agarose Resin (100ml) Fastback-IDA-100 100 ml PROTEIN ARK
Fastback IDA Agarose Resin (1L) Fastback-IDA-1000 1 litre PROTEIN ARK
Fastback IDA Agarose Resin (250ml) Fastback-IDA-250 250 ml PROTEIN ARK
Fastback IDA Agarose Resin (500ml) Fastback-IDA-500 500 ml PROTEIN ARK
Fastback IDA Agarose Resin (5L) Fastback-IDA-5000 5 litre PROTEIN ARK
Fastback Ni Advance Resin (10 ml) Fastback-Ni-Adv-10 10 ml PROTEIN ARK
Fastback Ni Advance Resin (100 ml) Fastback-Ni-Adv-100 100 ml PROTEIN ARK
Fastback Ni Advance Resin (25 ml) Fastback-Ni-Adv-25 25 ml PROTEIN ARK
Fastback Ni IMAC Resin (10 ml) Fastback-Ni-10 10 ml PROTEIN ARK
Fastback Ni IMAC Resin (100 ml) Fastback-Ni-100 100 ml PROTEIN ARK
Fastback Ni IMAC Resin (25 ml) Fastback-Ni-25 25 ml PROTEIN ARK
Fastback Zinc NTA Agarose Resin (100ml) Fastback-Zn-NTA-100 100 ml PROTEIN ARK
Fastback Zinc NTA Agarose Resin (1L) Fastback-Zn-NTA-1000 1 litre PROTEIN ARK
Fastback Zinc NTA Agarose Resin (25ml) Fastback-Zn-NTA-25 25 ml PROTEIN ARK
Magnetic Co Affinity Beads (5ml) Magnetic-Co-5ml 5 ml PROTEIN ARK
Magnetic Ni Affinity Beads (5ml) Magnetic-Ni-5ml 5 ml PROTEIN ARK
Magnetic Zn Affinity Beads (5ml) Magnetic-Zn-5ml 5 ml PROTEIN ARK
Super Co-NTA Agarose Resin (10 ml) Super-CoNTA10 10 ml PROTEIN ARK
Super Co-NTA Agarose Resin (100 ml) Super-CoNTA100 100 ml PROTEIN ARK
Super Co-NTA Agarose Resin (25 ml) Super-CoNTA25 25 ml PROTEIN ARK
Super Ni-NTA Agarose Resin (10 ml) Super-NiNTA10 10 ml PROTEIN ARK
Super Ni-NTA Agarose Resin (100 ml) Super-NiNTA100 100 ml PROTEIN ARK
Super Ni-NTA Agarose Resin (25 ml) Super-NiNTA25 25 ml PROTEIN ARK
Super NTA Agarose Resin (100ml) Super-NTA-100 100 ml PROTEIN ARK
Super NTA Agarose Resin (1L) Super-NTA-1000 1 litre PROTEIN ARK
Super NTA Agarose Resin (25ml) Super-NTA-25 25 ml PROTEIN ARK
Super NTA Agarose Resin (5L) Super-NTA-5000 5 litre PROTEIN ARK
Super Zinc NTA Agarose Resin (100ml) Super-Zn-NTA-100 100 ml PROTEIN ARK
Super Zinc NTA Agarose Resin (1L) Super-Zn-NTA-1000 1 litre PROTEIN ARK
Super Zinc NTA Agarose Resin (25ml) Super-Zn-NTA-25 25 ml PROTEIN ARK
전체보기
Maker
PROTEIN ARK
Cat.No.
Fastback-Co-10
Product
Fastback Co IMAC Resin (10 ml)
Size
10 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Co-100
Product
Fastback Co IMAC Resin (100 ml)
Size
100 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Co-25
Product
Fastback Co IMAC Resin (25 ml)
Size
25 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-IDA-100
Product
Fastback IDA Agarose Resin (100ml)
Size
100 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-IDA-1000
Product
Fastback IDA Agarose Resin (1L)
Size
1 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-IDA-250
Product
Fastback IDA Agarose Resin (250ml)
Size
250 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-IDA-500
Product
Fastback IDA Agarose Resin (500ml)
Size
500 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-IDA-5000
Product
Fastback IDA Agarose Resin (5L)
Size
5 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-Adv-10
Product
Fastback Ni Advance Resin (10 ml)
Size
10 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-Adv-100
Product
Fastback Ni Advance Resin (100 ml)
Size
100 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-Adv-25
Product
Fastback Ni Advance Resin (25 ml)
Size
25 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-10
Product
Fastback Ni IMAC Resin (10 ml)
Size
10 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-100
Product
Fastback Ni IMAC Resin (100 ml)
Size
100 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Ni-25
Product
Fastback Ni IMAC Resin (25 ml)
Size
25 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Fastback-Zn-NTA-100
Product
Fastback Zinc NTA Agarose Resin (100ml)
Size
100 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-Zn-NTA-1000
Product
Fastback Zinc NTA Agarose Resin (1L)
Size
1 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Fastback-Zn-NTA-25
Product
Fastback Zinc NTA Agarose Resin (25ml)
Size
25 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Magnetic-Co-5ml
Product
Magnetic Co Affinity Beads (5ml)
Size
5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Magnetic-Ni-5ml
Product
Magnetic Ni Affinity Beads (5ml)
Size
5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Magnetic-Zn-5ml
Product
Magnetic Zn Affinity Beads (5ml)
Size
5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Super-CoNTA10
Product
Super Co-NTA Agarose Resin (10 ml)
Size
10 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-CoNTA100
Product
Super Co-NTA Agarose Resin (100 ml)
Size
100 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-CoNTA25
Product
Super Co-NTA Agarose Resin (25 ml)
Size
25 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-NiNTA10
Product
Super Ni-NTA Agarose Resin (10 ml)
Size
10 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-NiNTA100
Product
Super Ni-NTA Agarose Resin (100 ml)
Size
100 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-NiNTA25
Product
Super Ni-NTA Agarose Resin (25 ml)
Size
25 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
Super-NTA-100
Product
Super NTA Agarose Resin (100ml)
Size
100 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Super-NTA-1000
Product
Super NTA Agarose Resin (1L)
Size
1 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Super-NTA-25
Product
Super NTA Agarose Resin (25ml)
Size
25 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Super-NTA-5000
Product
Super NTA Agarose Resin (5L)
Size
5 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Super-Zn-NTA-100
Product
Super Zinc NTA Agarose Resin (100ml)
Size
100 ml
Qty
Maker
PROTEIN ARK
Cat.No.
Super-Zn-NTA-1000
Product
Super Zinc NTA Agarose Resin (1L)
Size
1 litre
Qty
Maker
PROTEIN ARK
Cat.No.
Super-Zn-NTA-25
Product
Super Zinc NTA Agarose Resin (25ml)
Size
25 ml
Qty
전체보기

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

  • PROTEIN ARK CATALOG

    PROTEIN ARK CATALOG 이미지

    [PROTEIN ARK]

    DNA 전기영동, 단백질 발현, 단백질 정제 및 분리 등에 사용할 수 있는 PROTEIN ARK의 제품을 소개합니다.

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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Data Sheet
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