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제품 상세
단백질 정제용 컬럼 | HiFliQ FPLC Column | Empty FliQ Column 이미지

단백질 정제용 컬럼 | HiFliQ FPLC Column | Empty FliQ Column

HiFliQ FPLC Column은 AKTA FPLC 와 같은 Liquid chromatography system에 사용할수 있는 column으로, Resin이 충전되어 있습니다. resin이 충전되지 않은 빈 타입의 컬럼으로도 제공합니다.

프린트 제품 문의
HiFliQ FPLC Columns

  

 

  • Resin이 충전되어 있어 바로 사용이 가능한 FPLC column 입니다.
  • 1ml, 5ml 사이즈로 제공됩니다.
  • bead size가 작고, binding capacity가 높습니다.
  • AKTA를 포함한 모든 chromatography 장치에 호환됩니다.
  • Nickel-NTA resin의 경우, 많은 양의 DTT에도 resistant 합니다. 
  • Heparin HP (High Performance) resin은 small sized bead로, 보다 높은 Binding capacity를 제공합니다.
  • 최상급의 resin을 사용하며, 목적에 따라 resin 선택의 폭이 넓습니다.

  

 

용도에 따른 종류

1) Nickel-(NI) NTA Agarose : His-tagged Protein Purification
2) Cobalt-(CO) NTA Agarose : His-tagged Protein Purification
3) Glutathione (GST) Agarose : GST-tagged Protein Purification
4) Protein A Agarose : IgG Purification
5) Protein G Agarose : IgG Purification
6) Heparin Agarose : Native Protein Purification 
7) Quaternary ammonium group (Q) coupled Agarose : rapid Ion Exchange (IEX) purification of negatively charged proteins under native conditions
8) Sulphonic acid propyl linked (S) coupled Agarose : rapid Ion Exchange (IEX) purification of positively charged proteins under native conditions  

 

　 Co-NTA GST Heparin Ni Advance Ni-NTA Protein A Protein G Q IEX Agarose S IEX Agarose
 1ml HiFliQ1-CoNTA-1 HiFliQ1-GST-1 HiFliQ1-HEP-1 HiFliQ1-Ni-Adv-1 HiFliQ1-NiNTA-1 HiFliQ1-PA-1 HiFliQ1-PG-1 HiFliQ1-Q-1 HiFliQ1-S-1
 1ml x 5 HiFliQ1-CoNTA-5  HiFliQ1-GST-5  HiFliQ1-HEP-5 HiFliQ1-Ni-Adv-5 HiFliQ1-NiNTA-5  HiFliQ1-PA-5  HiFliQ1-PG-5  HiFliQ1-Q-5 HiFliQ1-S-5
 5ml HiFliQ5-CoNTA-1 HiFliQ5-GST-1 HiFliQ5-HEP-1 HiFliQ5-Ni-Adv-1 HiFliQ5-NiNTA-1 HiFliQ5-PA-1 HiFliQ5-PG-1 HiFliQ5-Q-1 HiFliQ5-S-1
 5ml x 5  HiFliQ5-CoNTA-5 HiFliQ5-GST-5 HiFliQ5-HEP-5 HiFliQ5-Ni-Adv-5 HiFliQ5-NiNTA-5 HiFliQ5-PA-5 HiFliQ5-PG-5  HiFliQ5-Q-5 HiFliQ5-S-5
 10ml HiFLiQ10-CoNTA-1 HiFLiQ10-GST-1 HiFLiQ10-HEP-1 HiFLiQ10-NiAdv-1 HiFliQ10-NiNTA-1 HiFLiQ10-PA-1 HiFLiQ10-PG-1 HiFLiQ10-Q-1 HiFLiQ10-S-1
 20ml HiFLiQ20-CoNTA-1 HiFLiQ20-GST-1 HiFLiQ20-HEP-1 HiFLiQ20-NiAdv-1 HiFliQ20-NiNTA-1 HiFLiQ20-PA-1 HiFLiQ20-PG-1 HiFLiQ20-Q-1 HiFLiQ20-S-1
 50ml HiFLiQ50-CoNTA-1 HiFLiQ50-GST-1 HiFLiQ50-HEP-1 HiFLiQ50-NiAdv-1 HiFliQ50-NiNTA-1 HiFLiQ50-PA-1 HiFLiQ50-PG-1 HiFLiQ50-Q-1 HiFLiQ50-S-1

 

 

 



Purification of DNA-binding protein from E.coli lysate on a 1 ml HiFliQ HEPARIN FPLC column. 
(A) FPLC chromatogram (B) SDS-PAGE of purification fractions using NuPage Gel (4-12% gradient), stained with Quick Coomassie.




Purification of GST-tagged protein from E.coli lysate on a 1 ml HiFliQ GST FPLC column. 
(A) FPLC chromatogram (B) SDS-PAGE analysis

 

Empty FliQ FPLC Columns



  • Empty 1 ml, 5 ml, 10 ml FPLC chromatography columns 입니다. 
  • 원하시는 resin으로 충전하여 사용할 수 있습니다. 
  • Universal 10.32 UNF threads
  • Compatible with FPLC & HPLC
  • Flow rate: 0.5 to 2 ml/min
  • Stability: pH 2-14
  • Max pressure: 70 psi (5 bar) (1 ml), 42 psi (3 bar) (5, 10, 20 ml)

 Cat. No.  Product  Packaging  구성품
 GEN-FliQ1  1ml FliQ Column (1 pc)  1ml x 1   1 Column Body, 2 End Adaptors, 2 Stop Plugs
 GEN-FliQ5  5ml FliQ Column (1 pc)  5ml x 1   1 Column Body, 2 End Adaptors, 2 Stop Plugs
 GEN-FliQ10  10ml FliQ Column (1 pc)  10ml x 1  1 Column Body, 2 End Adaptors, 2 Stop Plugs

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
1 ml HiFliQ Co-NTA FPLC column (1 x 1 ml) HiFliQ1-CoNTA-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Co-NTA FPLC columns (5 x 1 ml) HiFliQ1-CoNTA-5 5x1 ml PROTEIN ARK
1 ml HiFliQ GST FPLC column (1 x 1 ml) HiFliQ1-GST-1 1x1 ml PROTEIN ARK
1 ml HiFliQ GST FPLC columns (5 x 1 ml) HiFliQ1-GST-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Heparin HP FPLC column (1 x 1 ml) HiFliQ1-HEP-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Heparin HP FPLC columns (5 x 1 ml) HiFliQ1-HEP-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Ni Advance FPLC column (1 x 1 ml) HiFliQ1Ni-Adv-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Ni Advance FPLC columns (5 x 1 ml) HiFliQ1Ni-Adv-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Ni-NTA FPLC column (1 x 1 ml) HiFliQ1-NiNTA-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Ni-NTA FPLC columns (5 x 1 ml) HiFliQ1-NiNTA-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Protein A FPLC column (1 x 1 ml) HiFliQ1-PA-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Protein A FPLC columns (5 x 1 ml) HiFliQ1-PA-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Protein G FPLC column (1 x 1 ml) HiFliQ1-PG-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Protein G FPLC columns (5 x 1 ml) HiFliQ1-PG-5 5x1 ml PROTEIN ARK
1 ml HiFliQ Q IEX Agarose FPLC column (1 x 1 ml) HiFliQ1-Q-1 1x1 ml PROTEIN ARK
1 ml HiFliQ Q IEX Agarose FPLC column (5 x 1 ml) HiFliQ1-Q-5 5x1 ml PROTEIN ARK
1 ml HiFliQ S IEX Agarose FPLC column (1 x 1 ml) HiFliQ1-S-1 1x1 ml PROTEIN ARK
1 ml HiFliQ S IEX Agarose FPLC column (5 x 1 ml) HiFliQ1-S-5 5x1 ml PROTEIN ARK
5 ml HiFliQ Co-NTA FPLC column (1 x 5 ml) HiFliQ5-CoNTA-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Co-NTA FPLC columns (5 x 5 ml) HiFliQ5-CoNTA-5 5x5 ml PROTEIN ARK
5 ml HiFliQ GST FPLC column (1 x 5 ml) HiFliQ5-GST-1 1x5 ml PROTEIN ARK
5 ml HiFliQ GST FPLC columns (5 x 5 ml) HiFliQ5-GST-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Heparin HP FPLC column (1 x 5 ml) HiFliQ5-HEP-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Heparin HP FPLC columns (5 x 5 ml) HiFliQ5-HEP-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Ni Advance FPLC columns (5 x 5 ml) HiFliQ5-Ni-Adv-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Ni Advance HP FPLC column (1 x 5 ml) HiFliQ5-Ni-Adv-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Ni-NTA FPLC column (1 x 5 ml) HiFliQ5-NiNTA-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Ni-NTA FPLC columns (5 x 5 ml) HiFliQ5-NiNTA-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Protein A FPLC column (1 x 5 ml) HiFliQ5-PA-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Protein A FPLC columns (5 x 5 ml) HiFliQ5-PA-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Protein G FPLC column (1 x 5 ml) HiFliQ5-PG-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Protein G FPLC columns (5 x 5 ml) HiFliQ5-PG-5 5x5 ml PROTEIN ARK
5 ml HiFliQ Q IEX Agarose FPLC column (1 x 5 ml) HiFliQ5-Q-1 1x5 ml PROTEIN ARK
5 ml HiFliQ Q IEX Agarose FPLC column (5 x 5 ml) HiFliQ5-Q-5 5x5 ml PROTEIN ARK
5 ml HiFliQ S IEX Agarose FPLC column (1 x 5 ml) HiFliQ5-S-1 1x5 ml PROTEIN ARK
5 ml HiFliQ S IEX Agarose FPLC column (5 x 5 ml) HiFliQ5-S-5 5x5 ml PROTEIN ARK
5ml FliQ Column (1 pc) GEN-FliQ5 1x5 ml PROTEIN ARK
10.32 packing connector (1 pc) GEN-10.32 1 PK PROTEIN ARK
10ml FliQ Column (1 pc) GEN-FliQ10 1x10 ml PROTEIN ARK
1ml FliQ Column (1 pc) GEN-FliQ1 1x1 ml PROTEIN ARK
전체보기
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-CoNTA-1
Product
1 ml HiFliQ Co-NTA FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-CoNTA-5
Product
1 ml HiFliQ Co-NTA FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-GST-1
Product
1 ml HiFliQ GST FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-GST-5
Product
1 ml HiFliQ GST FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-HEP-1
Product
1 ml HiFliQ Heparin HP FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-HEP-5
Product
1 ml HiFliQ Heparin HP FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1Ni-Adv-1
Product
1 ml HiFliQ Ni Advance FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1Ni-Adv-5
Product
1 ml HiFliQ Ni Advance FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-NiNTA-1
Product
1 ml HiFliQ Ni-NTA FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-NiNTA-5
Product
1 ml HiFliQ Ni-NTA FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-PA-1
Product
1 ml HiFliQ Protein A FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-PA-5
Product
1 ml HiFliQ Protein A FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-PG-1
Product
1 ml HiFliQ Protein G FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-PG-5
Product
1 ml HiFliQ Protein G FPLC columns (5 x 1 ml)
Size
5x1 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-Q-1
Product
1 ml HiFliQ Q IEX Agarose FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-Q-5
Product
1 ml HiFliQ Q IEX Agarose FPLC column (5 x 1 ml)
Size
5x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-S-1
Product
1 ml HiFliQ S IEX Agarose FPLC column (1 x 1 ml)
Size
1x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ1-S-5
Product
1 ml HiFliQ S IEX Agarose FPLC column (5 x 1 ml)
Size
5x1 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-CoNTA-1
Product
5 ml HiFliQ Co-NTA FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-CoNTA-5
Product
5 ml HiFliQ Co-NTA FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-GST-1
Product
5 ml HiFliQ GST FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-GST-5
Product
5 ml HiFliQ GST FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-HEP-1
Product
5 ml HiFliQ Heparin HP FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-HEP-5
Product
5 ml HiFliQ Heparin HP FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-Ni-Adv-5
Product
5 ml HiFliQ Ni Advance FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-Ni-Adv-1
Product
5 ml HiFliQ Ni Advance HP FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-NiNTA-1
Product
5 ml HiFliQ Ni-NTA FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-NiNTA-5
Product
5 ml HiFliQ Ni-NTA FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-PA-1
Product
5 ml HiFliQ Protein A FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-PA-5
Product
5 ml HiFliQ Protein A FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-PG-1
Product
5 ml HiFliQ Protein G FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-PG-5
Product
5 ml HiFliQ Protein G FPLC columns (5 x 5 ml)
Size
5x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-Q-1
Product
5 ml HiFliQ Q IEX Agarose FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-Q-5
Product
5 ml HiFliQ Q IEX Agarose FPLC column (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-S-1
Product
5 ml HiFliQ S IEX Agarose FPLC column (1 x 5 ml)
Size
1x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
HiFliQ5-S-5
Product
5 ml HiFliQ S IEX Agarose FPLC column (5 x 5 ml)
Size
5x5 ml
Qty
Data Sheet
Maker
PROTEIN ARK
Cat.No.
GEN-FliQ5
Product
5ml FliQ Column (1 pc)
Size
1x5 ml
Qty
Maker
PROTEIN ARK
Cat.No.
GEN-10.32
Product
10.32 packing connector (1 pc)
Size
1 PK
Qty
Maker
PROTEIN ARK
Cat.No.
GEN-FliQ10
Product
10ml FliQ Column (1 pc)
Size
1x10 ml
Qty
Maker
PROTEIN ARK
Cat.No.
GEN-FliQ1
Product
1ml FliQ Column (1 pc)
Size
1x1 ml
Qty
전체보기

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관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

  • PROTEIN ARK CATALOG

    PROTEIN ARK CATALOG 이미지

    [PROTEIN ARK]

    DNA 전기영동, 단백질 발현, 단백질 정제 및 분리 등에 사용할 수 있는 PROTEIN ARK의 제품을 소개합니다.

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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Cat.No.
Product
Size
Qty
Data Sheet
MSDS

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