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rRNA 제거를 위한 riboPOOL 제작서비스 이미지

rRNA 제거를 위한 riboPOOL 제작서비스

riboPOOL은 다양한 species의 ribosomal RNA와 abundant RNA를 효과적으로 제거하기 위한 DNA probe pool로, RNA-seq의 효율을 높여줍니다. 원하시는 species에 대한 riboPOOL을 제작해 드립니다.

특징 

 

  • 제거하고자 하는 Ribosomal RNA에 hybridization 하기 위한 oliginucleotide pooling mixture를 제작해 드립니다. 
  • Cytoplasmic, mitochondiral 리보좀 RNA를 효과적으로 제거하며 95%이상의 rRNA depletion efficiency를 갖습니다.
  • Biotin이 coupling되어 있어, Streptavidin-coated magnetic bead를 이용한 target rRNA의 capture, removal이 수월합니다. 
  • Ribo-Zero (Illumina)에 비해 rRNA 제거효율이 높습니다.
  • human/mouse/rat 및 bacteria, archaea, plant, fungi 등 다양한 species에 대한 riboPOOL probe를 제작합니다. 
  • Ready-Made riboPOOL로 제공되거나, 원하는 species가 없을 경우 custom으로 제작해 드립니다. 
  • 단일 riboPOOL 혹은 분석에 필요한 시약이 함께 제공되는 riboPOOL kit 으로 구매하실 수 있습니다.  ​ 

 

>>> Ready-Made riboPOOL rRNA depletion kits 보러가기

  

 

 

 riboPOOL Workflow


 

 

 

 

실험 데이터 

  


 

 


 

 

Data with Pan-Plant riboPOOL – Efficient Across Phyla

 

 

 

Data kindly provided by Dr. Bruno Huettel and Dr. Christian Wöhle, Max Planck Genome Centre Cologne. RNA (1 μg, RIN ~8) was isolated with QIAgen RNeasy and subject to rRNA depletion with Pan-Plant riboPOOL, followed by clean-up with RNA XPClean (Beckman Coulter). Library preparation and sequencing was performed with NEB kit (modified) and Illumina HiSeq3000 respectively. 

 

  

 

사용 논문 

 

  • Al-Jourani et al. (2023) Identification of D-arabinan-degrading enzymes in mycobacteria Nature communications
  • Hadjeras et al. (2023) Unraveling the small proteome of the plant symbiont Sinorhizobium meliloti by ribosome profiling and proteogenomics microLife
  • Li et al. (2023) Differential regulation of mRNA stability modulates transcriptional memory and facilitates environmental adaptation Nature Communications
  • Hadjeras et al. (2023) Revealing the small proteome of Haloferax volcanii by combining ribosome profiling and small-protein optimized mass spectrometry microLife
  • Matiasek et al. (2023) Mystery of fatal ‘staggering disease’ unravelled: novel rustrela virus causes severe meningoencephalomyelitis in domestic cats Nature communications
  • Steiner et al. (2023) Central Role of Sibling Small RNAs NgncR_162 and NgncR_163 in Main Metabolic Pathways of Neisseria gonorrhoeae mBio
  • Sahonero-Canavesi et al. (2022) Disentangling the lipid divide: Identification of key enzymes for the biosynthesis of membrane-spanning and ether lipids in Bacteria Science Advances
  • Zhang et al.  (2022) A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis Plant Methods 
  • Palomar-Siles et al. (2022) Translational readthrough of nonsense mutant TP53 by mRNA incorporation of 5-Fluorouridine Cell Death & Disease
  • Snieckute et al. (2022) Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response Cell Metabolism
  • Kim et al. (2022)  Characterization of an Entner–Doudoroff pathway-activated Escherichia coli  Biotechnology for Biofuels and Bioproducts
  • Cannon et al. (2022) Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 PLOS Pathogens
  • Fernández-Justel et al. (2022) Histone H1 regulates non-coding RNA turnover on chromatin in a m6A-dependent manner Cell Reports
  • Meindl et al. (2022) A rapid protocol for ribosome profiling of low input samples bioRxiv also cites siFractor
  • Li et al. (2022) Sensing of individual stalled 80S ribosomes by Fap1 for nonfunctional rRNA turnover Molecular Cell
  • Fritz et al. (2022) Transcriptomic fingerprint of bacterial infection in lower extremity ulcers APMIS
  • Chen et al. (2022) Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq STAR Protocols
  • Jia et al. (2022) An atlas of plant full-length RNA reveals tissue-specific and monocots–dicots conserved regulation of poly(A) tail length Nature Plants
  • Alarcón-Schumacher et al. (2022) Isolation of a virus causing a chronic infection in the archaeal model organism Haloferax volcanii reveals antiviral activities of a provirus PNAS
  • Waditee‑Sirisattha et al. (2022) Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418 Scientific Reports
  • Jung et al. (2022) Opportunities and challenges to profile mRNA modifications in Escherichia coli ChemBioChem
  • Goar et al. (2022) Molecular Connectivity between Extracytoplasmic Sigma Factors and PhoP Accounts for Coupled Mycobacterial Stress Response Journal of Bacteriology
  • Pastor et al. (2022) Comparative Analysis of rRNA Removal Methods for RNA-Seq Differential Expression in Halophilic Archaea Biomolecules
  • Wahl et al. (2022) Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing Scientific Reports
  • Wittekind et al. (2022) The novel protein ScrA acts through the SaeRS two component system to regulate virulence gene expression in Staphylococcus aureus Molecular Microbiology
  • Fujii et al. (2022) Ribotype-dependent growth inhibition and promotion by erythritol in Cutibacterium acnes Journal of Cosmetic Dermatology
  • Puiggené, Ò. et al. (2022) Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1 Scientific Reports
  • M. A. Lawlo et al. (2021) A transposon expression burst accompanies the activation of Y-chromosome fertility genes during Drosophila spermatogenesis Nature Communications
  • Felix Grünberger et al. (2021) Nanopore sequencing of RNA and cDNA molecules in Escherichia coli RNA
  • Martin Gélinas et al. (2021) The de novo Purine Biosynthesis Pathway Is the Only Commonly Regulated Cellular Pathway during Biofilm Formation in TSBBased Medium in Staphylococcus aureus and Enterococcus faecalis Microbiology Spectrum
  • Weipeng Mo et al. (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing Genome Biology
  • Dimitar Plamenov Petrov et al. (2021) Quantitative  profiling  and  dynamics  of  mRNA  modifications  in  Escherichia coli
  • Bhat et al. (2021) Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome Science
  • Mio Takeuchi, Hideyoshi Yoshioka (2021) Acetate excretion by a methanotroph, Methylocaldum marinum S8, under aerobic conditions Bioscience, Biotechnology, and Biochemistry
  • Blaze et al (2021) Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior Nature Communications
  • Y. Long (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing Nature Protocols
  • Neil Fleck & Christoph Grundner (2021) A Cas12a-based CRISPR interference system for multigene regulation in mycobacteria JBC
  • J. Andersen et al (2021) Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms npj Biofilms and Microbiomes
  • La Rosa et al (2021) Compensatory evolution of Pseudomonas aeruginosa’s slow growth phenotype suggests mechanisms of adaptation in cystic fibrosis Nature Communications 
  • Fuchs et al (2021) Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach PLOS Genetics
  • Martin Benda (2020) PhD dissertation A possible functional link between RNA degradation and transcription in Bacillus subtilis
  • Ryan. D et. al (2020) A high-resolution transcriptome map identifies small RNA regulation of metabolism in the gutmicrobe Bacteroides thetaiotaomicron. Nature Communications 
  • Kim, I et. al (2019) Efficient depletion of ribosomal RNA for RNA sequencing in planarians. BMC Genomics 
  • Galmozzi, C. V. et. al (2019) Selective ribosome profiling to study interactions of translating ribosomes in yeast. Nature Protocols   


Note: This service is for Research Use Only. Not for use in diagnostic procedures. 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
riboPOOL 제작서비스 . . SITOOL
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자료

  • Microbiome Brochure

    Microbiome Brochure 이미지

    [고마바이오텍]

    Microbiome 연구에 필요한 제품을 소개합니다.

  • Pan-Prokaryote riboPOOL

    Pan-Prokaryote riboPOOL 이미지

    [SITOOLS]

    Pan-Prokaryote riboPOOL에 대한 안내입니다.

  • Ready-Made and Custom riboPOOL

    Ready-Made and Custom riboPOOL 이미지

    [SITOOLS]

    Ready-made riboPOOL 목록과 서비스에 대한 안내입니다.

  • riboPOOL

    riboPOOL 이미지

    [SITOOLS]

    riboPOOL에 대한 안내입니다.

웨비나/Video

  • [SITOOL] riboPOOL™ for efficient and flexible ribosomal RNA depletion 이미지

    [SITOOL] riboPOOL™ for efficient and flexible ribosomal RNA depletion

    ribosomal RNA를 제거하기 위한 효과적인 툴인 riboPOOL에 대한 웨비나 영상입니다.

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0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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