raPOOL 제작서비스 | 타겟 RNA pool down | 고마바이오텍

특징

• raPOOL은 Biotinylated antisense DNA oligonucleotide 30개가 pooling된 mixture로,  RNA-Protein complex를 pull-down 시키는 툴 입니다.
  
• non-coding RNA와 상호작용하는 단백질, DNA, RNA (interactome)등을 분석하는데 필수적입니다. (예, lncRNA, piRNA)
  
• 타겟 RNA에 대해 specific하며 회수율이 100배 이상 높습니다.
  
• RNA enrichment에 재현성과 일관성이 있습니다.
• 제공 : 
• HPLC purified 3'-biotinylated ssDNA (raPOOLs)
• Positive control raPOOLs (positive control: MALAT1)

raPOOL Performance

Lysates from HeLa cells were incubated with two different raPOOLs against human MALAT1, human XIST or E. coli LacZ (Negative control) for 4 h at 37°C. Following RNA isolation and cDNA synthesis, levels of Cyclophilin A (PPIA), GAPDH or targeted lncRNA, MALAT1, were measured by real-time quantitative PCR. Approximately 100-fold RNA enrichment of the target (MALAT1) was detected that was reproduced by both raPOOLs. Enrichment was specific to the targeted RNA.