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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

 

ID Microorganism riboPOOL kits ID Microorganism riboPOOL kits
026 Pan-Bacteria  019 Pichia pastoris 
027 Pan-Archaea  018 Pseudomonas aeruginosa 
003 Pan-Prokaryote   005 Saccharomyces cerevisiae 
043 Pan-Fungi  022 Salmonella enterica 
012 Bacillus subtilis  059 Schizosaccharomyces pombe 
013 Caulobacter crescentus  021 Staphylococcus aureus 
015 Clostridium perfringens  023 Stenotrophomonas sp. 
084 Cryptococcus neoformans  024 Ustilago maydis 
004 Escherichia coli  086 Wolbachia pipientis 
016 Haloferax volcanii 

 

  


특징   

 

  • riboPOOL kit은 siTOOL사의 Pack Hunter design 알고리즘을 기반으로 complexity를 고도로 반영하여 제작한 siRNA pool 입니다. 
  • riboPOOL은 최적의 최대 rRNA 커버리지를 보장하며, 거의 모든 종의 rRNA 및 abundant RNA를 제거합니다. 
  • Single species (예, Homo sapiens, Drosophila melanogaster등) 및 multi-species (예, Pan-prokaryote, Pan-Bacteria등)으로 선택하실 수 있습니다. 
  • ribosome profiling (Ribo-Seq) 전용 Ribo-Seq riboPOOL 및, FFPE와 같이 RNA가 분해되기 쉬운 샘플 (highly degraded samples)에 전용으로 사용되는 riboPOOL을 제공합니다. 
  • 6rx / 12rx / 24rx / 96rx kit으로 제공됩니다.
  • kit을 이용한 rRNA 제거에 소요되는 시간은 70분이며, RNA input range는 10ng-3ug 입니다. (10ug까지 scale up 가능)
  • Non-depleted 샘플과 depleted sample간 높은 correlation (R > 0.9)을 가집니다.



적용

  • Metatranscriptomics with Pan-Prokaryote riboPOOL
  • Depletion of abundant RNAs
  • Ribosome profiling
  • Depletion of rRNAs from multiple species with Combination riboPOOLs


riboPOOL Kit의 구성 

　 6rxn Trial Kit 12 rxn kit 24 rxn kit 96 rxn kit
riboPOOL 1 x lyophilized powder 1 x lyophilized powder 1 x lyophilized powder 1 x lyophilized powder
Nuclease-free water 1 x 1ml 1 x 1ml 1 x 1ml 1 x 1ml
Hybridization buffer 1 x 1ml 1 x 1ml 1 x 1ml 1 x 1ml
Depletion buffer 1 x 8ml 1 x 8ml 1 x 8ml 2 x 8ml
Streptavidin-coated magnetic beads 1 x 0.6ml 1 x 1.2ml 2 x 1.2ml 8 x 1.2ml
1.5ml Reaction tubes 1 x 50 tubes 1 x 50 tubes 2 x 50 tubes 4 x 50 tubes
Linear acrylamide 1 x 30ul 1 x 30ul 1 x 30ul 1 x 110ul
Sodium acetate, 3M 1 x 300ul 1 x 300ul 1 x 300ul 1 x 1.1ml
Clean-up beads 1 x 1050ul 1 x 2ml 1 x 4ml 1 x 16ml

 

 

실험 과정

 

1. Preparation of siBeads & riboPOOL
2. Hybridization of riboPOOL to target RNA
3. rRNA depletion
4. RNA clean up

ribopool kit workflow



종류

1) Pan-riboPOOL 

  • Pan-riboPOOL은  Bacteria, Plant, Mammal등 각 계통에 속한 여러 species를 커버하는 riboPOOL을 제공합니다. single-step rRNA depletion을 통해 mono/multi-transcriptomic study를 효과적으로 진행합니다.
  • riboPOOL은 환경샘플, 혈액, 감염조직 등으로부터 rRNA를 제거합니다. 
  • 서로 다른 species의 2-4개 riboPOOL을 결합하면 간단한 메타전사체(metatranscriptomics)연구가 가능합니다. (예,  combination of mixed human & bacteria samples..)

2) Single-Species riboPOOLs
  • Single-species riboPOOL은 잘 연구되고 덜 알려진 species (Escherichia coli, Arabidopsis thaliana or Schmidtea mediterranea 등)에 사용될 수 있습니다.
  • Single-species riboPOOL은 conserved, non-conserved region을 모두 표적으로 하기 위해 종에 특이적인 rRNA를 기반으로 설계되었습늬다. 
  • rRNA depletion 효율이 높습니다. 

3) Special Applications riboPOOLs
  • RNA가 분해되기 쉬운 샘플 (예, FFPE)나 ribosome profiling (Ribo-Seq) 샘플, RNA mixture (예, seawater samples)를 이용한 샘플을 위한 riboPOOL 입니다. 



riboPOOL Kit 선택가이드  
* 원하시는 species 가 없을 경우, custom으로 제작해 드립니다.  제작서비스 바로가기 

  • Pan-riboPOOLs & Single species riboPOOLs

Group ID Species Cat. No.
(6rxn Trial Kit)		 Cat. No.
(12 rxn kit)		 Cat. No.
(24 rxn kit)		 Cat. No.
(96 rxn kit)
Archaea 016 Haloferax volcanii  dp-K006-0016 dp-K012-000016 dp-K024-000016 dp-K096-000016
027 Pan-Archaea  dp-K006-0027 dp-K012-000027 dp-K024-000027 dp-K096-000027
Arthropoda 047 Aedes albopictus  dp-K006-0047 dp-K012-000047 dp-K024-000047 dp-K096-000047
048 Aedes albopictus rPseudogenes  dp-K006-0048 dp-K012-000048 dp-K024-000048 dp-K096-000048
073 Apis mellifera  dp-K006-0073 dp-K012-000073 dp-K024-000073 dp-K096-000073
074 Argiope bruennichi  dp-K006-0074 dp-K012-000074 dp-K024-000074 dp-K096-000074
080 Bemisia tabaci  dp-K006-0080 dp-K012-000080 dp-K024-000080 dp-K096-000080
007 Drosophila melanogaster  dp-K006-0007 dp-K012-000007 dp-K024-000007 dp-K096-000007
030 Ixodes scapularis  dp-K006-0030 dp-K012-000030 dp-K024-000030 dp-K096-000030
072 Leptinotarsa decemlineata  dp-K006-0072 dp-K012-000072 dp-K024-000072 dp-K096-000072
020 Plautia stali (bug) dp-K006-0020 dp-K012-000020 dp-K024-000020 dp-K096-000020
085 Spodoptera exigua  dp-K006-0085 dp-K012-000085 dp-K024-000085 dp-K096-000085
081 Varroa destructor  dp-K006-0081 dp-K012-000081 dp-K024-000081 dp-K096-000081
Bacteria 012 Bacillus subtilis  dp-K006-0012 dp-K012-000012 dp-K024-000012 dp-K096-000012
013 Caulobacter crescentus  dp-K006-0013 dp-K012-000013 dp-K024-000013 dp-K096-000013
015 Clostridium perfringens  dp-K006-0015 dp-K012-000015 dp-K024-000015 dp-K096-000015
004 Escherichia coli  dp-K006-0004 dp-K012-000004 dp-K024-000004 dp-K096-000004
035 Pan-Actinobacteria  dp-K006-0035 dp-K012-000035 dp-K024-000035 dp-K096-000035
026 Pan-Bacteria  dp-K006-0026 dp-K012-000026 dp-K024-000026 dp-K096-000026
018 Pseudomonas aeruginosa  dp-K006-0018 dp-K012-000018 dp-K024-000018 dp-K096-000018
022 Salmonella enterica  dp-K006-0022 dp-K012-000022 dp-K024-000022 dp-K096-000022
021 Staphylococcus aureus  dp-K006-0021 dp-K012-000021 dp-K024-000021 dp-K096-000021
023 Stenotrophomonas sp.  dp-K006-0023 dp-K012-000023 dp-K024-000023 dp-K096-000023
086 Wolbachia pipientis  dp-K006-0086 dp-K012-000086 dp-K024-000086 dp-K096-000086
Bacteria & Archaea 003 Pan-Prokaryote   dp-K006-0003 dp-K012-000003 dp-K024-000003 dp-K096-000003
Fungi 084 Cryptococcus neoformans  dp-K006-0084 dp-K012-000084 dp-K024-000084 dp-K096-000084
006 Filamentous-Fungi  dp-K006-0006 dp-K012-000006 dp-K024-000006 dp-K096-000006
043 Pan-Fungi  dp-K006-0043 dp-K012-000043 dp-K024-000043 dp-K096-000043
019 Pichia pastoris  dp-K006-0019 dp-K012-000019 dp-K024-000019 dp-K096-000019
005 Saccharomyces cerevisiae  dp-K006-0005 dp-K012-000005 dp-K024-000005 dp-K096-000005
059 Schizosaccharomyces pombe  dp-K006-0059 dp-K012-000059 dp-K024-000059 dp-K096-000059
024 Ustilago maydis  dp-K006-0024 dp-K012-000024 dp-K024-000024 dp-K096-000024
Mollusca 063 Crassostrea gigas  dp-K006-0063 dp-K012-000063 dp-K024-000063 dp-K096-000063
017 Loripes orbiculatus and Lucinoma aequizonata (Clams)  dp-K006-0017 dp-K012-000017 dp-K024-000017 dp-K096-000017
089 Pan-Mussel  dp-K006-0088 dp-K012-000089 dp-K024-000089 dp-K096-000089
Nematoda 039 Caenorhabditis elegans  dp-K006-0039 dp-K012-000039 dp-K024-000039 dp-K096-000039
Plant 031 Pan-Plant  dp-K006-0031 dp-K012-000031 dp-K024-000031 dp-K096-000031
Plant & Algae 008 Arabidopsis thaliana  dp-K006-0008 dp-K012-000008 dp-K024-000008 dp-K096-000008
071 Chlamydomonas reinhardtii  dp-K006-0071 dp-K012-000071 dp-K024-000071 dp-K096-000071
075 Cyanidioschyzon merolae  dp-K006-0075 dp-K012-000075 dp-K024-000075 dp-K096-000075
033 Emiliania huxleyi (Algae) dp-K006-0033 dp-K012-000033 dp-K024-000033 dp-K096-000033
009 Oryza sativa  dp-K006-0009 dp-K012-000009 dp-K024-000009 dp-K096-000009
Platyhelminthes 090 Schistosoma mansoni  dp-K006-0090 dp-K012-000090 dp-K024-000090 dp-K096-000090
028 Schmidtea mediterranea  dp-K006-0028 dp-K012-000028 dp-K024-000028 dp-K096-000028
Sponges & Cnidarians 011 Amphimedon queenslandica  dp-K006-0011 dp-K012-000011 dp-K024-000011 dp-K096-000011
087 Nematostella vectensis  dp-K006-0087 dp-K012-000087 dp-K024-000087 dp-K096-000087
Vertebrata 014 Chinchilla lanigera  dp-K006-0014 dp-K012-000014 dp-K024-000014 dp-K096-000014
010 Danio rerio  dp-K006-0010 dp-K012-000010 dp-K024-000010 dp-K096-000010
060 Gallus gallus domesticus  dp-K006-0060 dp-K012-000060 dp-K024-000060 dp-K096-000060
054 human  dp-K006-0054 dp-K012-000054 dp-K024-000054 dp-K096-000054
098 human - cytoplasmatic rRNAs only dp-K006-0098 dp-K012-000098 dp-K024-000098 dp-K096-000098
094 human Blood  dp-K006-0094 dp-K012-000094 dp-K024-000094 dp-K096-000094
053 human/mouse/rat  dp-K006-0053 dp-K012-000053 dp-K024-000053 dp-K096-000053
091 Human+hGlobin+7SL [10:1:1]  dp-K006-0091 dp-K012-000091 dp-K024-000091 dp-K096-000091
055 mouse/rat  dp-K006-0055 dp-K012-000055 dp-K024-000055 dp-K096-000055
032 Pan-Bird  dp-K006-0032 dp-K012-000032 dp-K024-000032 dp-K096-000032
041 Pan-Mammal  dp-K006-0041 dp-K012-000041 dp-K024-000041 dp-K096-000041
Others 095 Anaeramoeba flamelloides  dp-K006-0095 dp-K012-000095 dp-K024-000095 dp-K096-000095
051 Blood Parasite  dp-K006-0051 dp-K012-000051 dp-K024-000051 dp-K096-000051
025 Human Globin mRNA  dp-K006-0025 dp-K012-000025 dp-K024-000025 dp-K096-000025
044 Pan-Sponge  dp-K006-0044 dp-K012-000044 dp-K024-000044 dp-K096-000044
034 Poly A (Poly-Adenylated RNAs)  dp-K006-0034 dp-K012-000034 dp-K024-000034 dp-K096-000034
038 SARS-CoV-2 RNA  dp-K006-0038 dp-K012-000038 dp-K024-000038 dp-K096-000038
068 Seawater  dp-K006-0068 dp-K012-000068 dp-K024-000068 dp-K096-000068
096 Thalassiosira pseudonana dp-K006-0096 dp-K012-000096 dp-K024-000096 dp-K096-000096


  • FFPE & degraded RNA sample을 위한 riboPOOLs

Group ID Species Cat. No.
(6rxn Trial Kit)		 Cat. No.
(12 rxn kit)		 Cat. No.
(24 rxn kit)		 Cat. No.
(96 rxn kit)
Arthropoda 061 Drosophila melanogaster degraded RNA  dp-K006-0061 dp-K012-000061 dp-K024-000061 dp-K096-000061
Nematoda 062 Caenorhabditis elegans degraded RNA  dp-K006-0062 dp-K012-000062 dp-K024-000062 dp-K096-000062
Vertebrata 092 Equus caballus degraded RNA  dp-K006-0092 dp-K012-000092 dp-K024-000092 dp-K096-000092
093 Felis catus degraded RNA  dp-K006-0093 dp-K012-000093 dp-K024-000093 dp-K096-000093
057 human FFPE/degraded RNA  dp-K006-0057 dp-K012-000057 dp-K024-000057 dp-K096-000057
056 human-mouse-rat FFPE/degraded RNA  dp-K006-0056 dp-K012-000056 dp-K024-000056 dp-K096-000056
058 mouse-rat FFPE/degraded RNA  dp-K006-0058 dp-K012-000058 dp-K024-000058 dp-K096-000058


  • Ribosome Profiling (Ribo-Seq)을 위한 riboPOOLs

Group ID Species Cat. No.
(6rxn Trial Kit)		 Cat. No.
(12 rxn kit)		 Cat. No.
(24 rxn kit)		 Cat. No.
(96 rxn kit)
Ribo-Seq 079 Azolla filiculoides ribo-seq  dp-K006-0079 dp-K012-000079 dp-K024-000079 dp-K096-000079
067 Caenorhabditis elegans ribo-seq  dp-K006-0067 dp-K012-000067 dp-K024-000067 dp-K096-000067
076 Drosophila melanogaster ribo-seq  dp-K006-0076 dp-K012-000076 dp-K024-000076 dp-K096-000076
042 human Ribo-Seq  dp-K006-0042 dp-K012-000042 dp-K024-000042 dp-K096-000042
050 human-mouse-rat Ribo-Seq  dp-K006-0050 dp-K012-000050 dp-K024-000050 dp-K096-000050
078 Leishmania mexicana ribo-seq  dp-K006-0078 dp-K012-000078 dp-K024-000078 dp-K096-000078
052 mouse-rat Ribo-Seq  dp-K006-0052 dp-K012-000052 dp-K024-000052 dp-K096-000052
082 Pristionchus pacificus Ribo-Seq  dp-K006-0082 dp-K012-000082 dp-K024-000082 dp-K096-000082
083 Toxoplasma gondii Ribo-Seq  dp-K006-0083 dp-K012-000083 dp-K024-000083 dp-K096-000083
077 Trypanosoma brucei ribo-seq  dp-K006-0077 dp-K012-000077 dp-K024-000077 dp-K096-000077





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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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