PEI STAR™ DNA Transfection Reagent | 고마바이오텍

특징

• PEI STAR는 일시적인 유전자 발현을 위한 polyethylenimine (PEI) transfection regent 입니다.
  
• PEI는 중성 pH에서 높은 양전하를 띠는 합성 폴리머로, 음전하를 띠는 DNA에 강하게 결합하여 세포내로 들어갈 수 있도록 합니다. 
• HEK293, CHO cell transfection에 이용됩니다. 
• PEI STAR-GO (#8174)는 바로 사용할 수 있는 용액 형태의 PEI STAR (#7854) 입니다.

사용법 

• PEI STAR™ Transfection Reagent preparation
• Gene Expression in Adherent HEK293 cells (Adherent cells)
• rAAV Production in Adherent HEK293 Cells (Adherent cells)
• Lentivirus Production in Adherent HEK293T Cells (Adherent cells)
• Gene Expression in HEK293 Cell Suspensions (Suspension cells)
• rAAV Production in Suspension HEK293 Cells (Suspension cells)
• Gene Expression in CHO Suspensions (Suspension cells)

참고 데이터

PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance. 

PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.