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제품 상세
Luciferase Reporter Gene Assay Kit 이미지

Luciferase Reporter Gene Assay Kit

Firefly & Renilla Luciferase를 분석하기 위한 Single Tube Assay, HTS Assay, Dual Luciferase Reporter Gene Assay Kit을 소개합니다.

프린트 제품 문의

Firefly & Renilla Luciferase Single Tube Assay Kit (#30081) 

 

  • Firefly와 Renilla luciferase는 reporter gene으로서 유전자의 조절(Regulation)이나 기능연구 , 신약 스크리닝을 위해 널리 사용됩니다.
  • Renilla Luciferase는 종종 multiplex transcriptional reporter assay에서 transfection 정도를 표준화(normalization)하는 컨트롤로써 Firefly luciferase와 함께 사용됩니다.
  • 다른 많은 효소들과 마찬가지로, Firefly luciferase와 Renilla luciferase는 Michaelis-Menten kinetics 방식을 따르므로 기질과 co-factor에의한 과정없이는 최대 빛 발산에 도달하지 않습니다. 이러한 조건으로 실험이 진행될 때, 반응으로부터 방출되는 빛은 luciferase 효소 분자수에 비례합니다.
  • Firefly & Renilla Luciferase Assay Kit은 배양세포 내에서 간편하고 효율적으로 Firefly와 Renilla luciferase reporter 효소의 정량 및 측정이 가능하도록 디자인되었습니다.
  • 구성품
    • 1X Passive Lysis Buffer 2.0
    • Firefly Luciferase Assay Buffer 2.0
    • D-Luciferin
    • Renilla Luciferase Assay Buffer 2.0
    • Aquaphile™ Coelenterazine

 

Firefly & Renilla Luciferase Single Tube Assay Kit (#30081)
 

 

 

Firefly Luciferase Assay Kit (#30075 & 30085) 


  • Transfected cell reporter assay에서 firefly luciferase 활성을 측정하기 위한 kit 입니다. 
  • culture cell로부터 luciferase reporter enzyme activity를 효과적으로 정량합니다.
  • 민감도가 향상된 flash-type luminescence assay의 version 2 (#30085)를 제공합니다. 
  • 구성품 
    • 5X Firefly Luciferase Lysis Buffer
    • D-Luciferin
    • Firefly Luciferase Assay Buffer (Lyophilized)


Firefly Luciferase Assay Kit (#30075 & 30085)


Steady-Luc™ Firefly HTS Assay Kit (#30028)

  • 약 3시간의 luminescence half-life를 갖는 homogeneous firefly luciferase reporter gene assay kit 입니다.
  • Multi-well plate에서의 high-throughput screening에 이용됩니다. 
  • 구성품
    • D-Luciferin
    • Steady-Luc™ Firefly Assay Buffer


Dual Luciferase Reporter Gene Assay Kit (#SLDL-100)

  • 하나의 샘플에서 2개의 다른 luciferase인 Firelfy(FFL)와 Ranilla(RL)의 활성을 순차적으로 측정하는 Kit입니다.
  • 좋은 민감도와 넓은 측정범위로 2 fg의 매우 적은양의 luciferase도 정확히 측정합니다.
  • 96-well plate 형식으로 제작되어 기존 HTS liquid handling systems보다 매우 빠르게 결과 확인이 가능합니다.
  • 단 3가지 step만으로 20분 이내에 2종류의 luciferase level을 측정할 수 있습니다.
  • 구성품
    • Lysis Buffer: 25 mL 
    • Assay Buffer: 25 mL 
    • FFL Reagent: 25 mg
    • RL Reagent: 400 mg

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
5X Firefly Luciferase Lysis Buffer 99923 15 ml BIOTIUM
Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 Assays 30081-2 1000 AS BIOTIUM
Firefly & Renilla Luciferase Single Tube Assay Kit, 150 Assays 30081-1 150 AS BIOTIUM
Firefly & Renilla Luciferase Single Tube Assay Kit, 50 Assays 30081-T 50 AS BIOTIUM
Firefly Luciferase Assay Kit (Lyophilized) 30075-1 150 TST BIOTIUM
Firefly Luciferase Assay Kit (Lyophilized) 30075-2 1000 TST BIOTIUM
Firefly Luciferase Assay Kit 2.0, 1000 Assays 30085-2 1000 AS BIOTIUM
Firefly Luciferase Assay Kit 2.0, 150 Assays 30085-1 150 AS BIOTIUM
Firefly Luciferase Assay Kit 2.0, 50 Assays 30085-T 50 AS BIOTIUM
Renilla Luciferase Assay Kit 2.0, 1000 Assays 30082-2 1000 AS BIOTIUM
Renilla Luciferase Assay Kit 2.0, 150 Assays 30082-1 150 AS BIOTIUM
Renilla Luciferase Assay Kit 2.0, 50 Assays 30082-T 50 AS BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) 10 x 1000 Assays 30028-L3 10000 TST BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 1000 Assays 30028-L2 1000 TST BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 120 Assays 30028-L1 120 TST BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit, 10 x 1000 Assays 30028-3 1 kit BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit, 1000 Assays 30028-2 1 kit BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit, 120 Assays 30028-1 1 kit BIOTIUM
Steady-Luc™ Firefly HTS Assay Kit, Trial Size, 40 Assays 30028-T 1 kit BIOTIUM
SuperLight™ Dual Luciferase Reporter Gene Assay Kit SLDL-100 100 assays BIOASSAY SYSTEMS
SuperLight™ Luciferase Reporter Gene Assay Kit SLLU-01K 1000 assays BIOASSAY SYSTEMS
SuperLight™ Luciferase Reporter Gene Assay Kit SLLU-500 500 assays BIOASSAY SYSTEMS
SuperLight™ Luciferase Reporter Gene Assay Kit SLLU-200 200 assays BIOASSAY SYSTEMS
전체보기
Maker
BIOTIUM
Cat.No.
99923
Product
5X Firefly Luciferase Lysis Buffer
Size
15 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30081-2
Product
Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 Assays
Size
1000 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30081-1
Product
Firefly & Renilla Luciferase Single Tube Assay Kit, 150 Assays
Size
150 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30081-T
Product
Firefly & Renilla Luciferase Single Tube Assay Kit, 50 Assays
Size
50 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30075-1
Product
Firefly Luciferase Assay Kit (Lyophilized)
Size
150 TST
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30075-2
Product
Firefly Luciferase Assay Kit (Lyophilized)
Size
1000 TST
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30085-2
Product
Firefly Luciferase Assay Kit 2.0, 1000 Assays
Size
1000 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30085-1
Product
Firefly Luciferase Assay Kit 2.0, 150 Assays
Size
150 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30085-T
Product
Firefly Luciferase Assay Kit 2.0, 50 Assays
Size
50 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30082-2
Product
Renilla Luciferase Assay Kit 2.0, 1000 Assays
Size
1000 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30082-1
Product
Renilla Luciferase Assay Kit 2.0, 150 Assays
Size
150 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30082-T
Product
Renilla Luciferase Assay Kit 2.0, 50 Assays
Size
50 AS
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-L3
Product
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) 10 x 1000 Assays
Size
10000 TST
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-L2
Product
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 1000 Assays
Size
1000 TST
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-L1
Product
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 120 Assays
Size
120 TST
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-3
Product
Steady-Luc™ Firefly HTS Assay Kit, 10 x 1000 Assays
Size
1 kit
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-2
Product
Steady-Luc™ Firefly HTS Assay Kit, 1000 Assays
Size
1 kit
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-1
Product
Steady-Luc™ Firefly HTS Assay Kit, 120 Assays
Size
1 kit
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
30028-T
Product
Steady-Luc™ Firefly HTS Assay Kit, Trial Size, 40 Assays
Size
1 kit
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
SLDL-100
Product
SuperLight™ Dual Luciferase Reporter Gene Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
SLLU-01K
Product
SuperLight™ Luciferase Reporter Gene Assay Kit
Size
1000 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
SLLU-500
Product
SuperLight™ Luciferase Reporter Gene Assay Kit
Size
500 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
SLLU-200
Product
SuperLight™ Luciferase Reporter Gene Assay Kit
Size
200 assays
Qty
Data Sheet
MSDS
전체보기

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관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

  • Luciferase Reporter Assays

    Luciferase Reporter Assays 이미지

    [BIOTIUM]

    Firefly & Renilla Luciferase Assay에 대한 안내입니다.

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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