siRNA Pool 제작서비스 | siPOOL™

특징

RNAi를 통한 knockdown은 cell의 유전자조작 없이 타겟을 inhibition할 수 있는 간편하고 빠른 방법입니다.
siPOOL은 타겟에 대한 30개의 siRNA mixture로, off-targeting을 5-25배까지 감소시키고, 1-3 nM의 양으로 70% 이상의 gene knock-down 효율을 제공합니다. Target gene ID를 제공하시면, 30개의 siRNA mixture pool을 제작해 드립니다.
다양한 Transfection reagent에 호환됩니다.
잘 디자인된 30개의 siRNA Pool을 사용함으로써 한번의 실험으로 논문용 결과를 얻으실 수 있습니다.
siRNA sequence information은 요청시 제공 가능합니다.

siPOOL 블로그

원리

3-4 개의 siRNA로 이루어진 pool은 종종 multiple off-target gene에 영향을 주는 반면, 30개의 siRNA Mixture로 이루어진 siPOOL은 picomolar에서 작용하며, on-target specificity를 높여줍니다.

실험 결과

Off-target effect를 낮출 수 있습니다.

(Transcriptome-wide profiling에 의하면 single siRNA가 여러 off-target genes(red dots)를 유발하는 결과가 나옴. 반면에 같은 target(green dot)에 대해 siPOOL이 off-target effect를 감소시켰음.)

일관적인 target Knock-down 효과를 보여줍니다.

(개별 single siRNA는 knock-down 효율의 편차가 큼. 같은 target에 대해 두 개의 다른 siPOOLs가 비슷한 knockdown efficiencies을 보임.)

siPOOLs로 일관적인 phenotypes를 재현할 수 있습니다.

(36 genes screening하였는데 gene당 두개의 siPOOL 과 commercial 3개의 siRNAs로 cell viability에 대한 phenotype을 분석하였음. siPOOLs가 더 일관적인 phenotype을 보여주었음.)

사용논문

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