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제품 상세
Cell Transformation Assay Kit 이미지

Cell Transformation Assay Kit

CytoSelect Cell Transformation Assay Kit는 semisolid agar에서 7-8일간 배양 후 cell transformation을 측정합니다. Colorimetric 및 Fluorometric format으로 제공되며 Cell Recovery Compatible Kit는 Transformed cell을 회수할 수 있습니다.

프린트 제품 문의

특징

 

  • semisolid agar에서 7-8일간 배양 후, cell transformation을 측정하는 제품입니다.  
  • 기존에 3-4주씩 걸리던 전통적인 soft agar colony 방식에 비해 좀더 신속하고 정확하게 cell transformation과 growth를 측정합니다.
  • 매뉴얼로 cell counting을 하지 않으므로, 정확한 정량이 가능합니다.
  • Cell colony 정량까지 약 6-8일 정도 소요됩니다. (기존 3-4주)



종류

  

CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation) (#CBA-130)


  • 기존 Soft agar assay에 비해 빠르고 정확성이 향상되었습니다.
  • Cell은 semisolid agar matrix 에서 1주 정도 배양 후, lysis를 거쳐 CyQuant® GR  형광 dye를 통해 fluorometric plate reader에서 측정됩니다.
  • Transformed cell은 회수되지 않습니다. (회수용으로는 Cell Recovery Compatible assay kit을 사용하세요.)

 


 

 

 


CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) (#CBA-135 / CBA-140)

  • Modified soft agar를 사용함으로써, cell colony를 회수해서 downstream analysis에 이용할 수 있습니다.
  • Transformed cells은 protein/DNA array analysis나 cancer vaccine development 같은 이후 연구를 위해 회수가 가능합니다.
  • Semisolid agar matrix 에서 1주일 정도 배양된 세포들은 fluorometric plate reader에서 측정되거나,  3D culture medium으로부터 회수가 가능합니다.
  • Colorimetric (#CBA-135) 및 fluorometric (#CBA-140) format으로 제공됩니다.
    



사용 논문

CBA-130 　
    1. Andriolo, G. et al. (2021). GMP-Grade Methods for Cardiac Progenitor Cells: Cell Bank Production and Quality Control. Methods Mol Biol. doi: 10.1007/7651_2020_286.
    2. Tan, T.T. et al. (2021). Assessment of Tumorigenic Potential in Mesenchymal-Stem/Stromal-Cell-Derived Small Extracellular Vesicles (MSC-sEV). Pharmaceuticals. 14(4):345. doi: 10.3390/ph14040345.
    3. Lo, E.K.K. et al. (2021). Low dose of zearalenone elevated colon cancer cell growth through G protein-coupled estrogenic receptor. Sci Rep. 11(1):7403. doi: 10.1038/s41598-021-86788-w.
    4. Park, S. et al. (2021). Cerebral Cavernous Malformation 1 Determines YAP/TAZ Signaling-Dependent Metastatic Hallmarks of Prostate Cancer Cells. Cancers (Basel). 13(5):1125. doi: 10.3390/cancers13051125.
    5. Huang, S.B. et al. (2021). Androgen deprivation-induced elevated nuclear SIRT1 promotes prostate tumor cell survival by reactivation of AR signaling. Cancer Lett. doi: 10.1016/j.canlet.2021.02.008.
    6. Gao, C. et al. (2020). High intratumoral expression of eIF4A1 promotes epithelial-to-mesenchymal transition and predicts unfavorable prognosis in gastric cancer. Acta Biochim Biophys Sin (Shanghai). pii: gmz168. doi: 10.1093/abbs/gmz168.
    7. Eckerdt, F.D. et al. (2020). Combined PI3Kα-mTOR Targeting of Glioma Stem Cells. Sci Rep. 10(1):21873. doi: 10.1038/s41598-020-78788-z.
    8. Byun, H.J. et al. (2020). LUCAT1 Epigenetically Downregulates the Tumor Suppressor Genes CXXC4 and SFRP2 in Gastric Cancer. Yonsei Med J. 61(11):923-934. doi: 10.3349/ymj.2020.61.11.923.
    9. Seo, H.G. et al. (2020). Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion. Cell Death Dis. 11(9):815. doi: 10.1038/s41419-020-02999-5.
    10. Chen, J. et al. (2020). Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). Acta Pharm Sin B. doi: 10.1016/j.apsb.2020.07.011.
    11. Inoue, S. et al. (2020). Diffuse mesothelin expression leads to worse prognosis through enhanced cellular proliferation in colorectal cancer. Oncol Lett. 19:1741-1750. doi: 10.3892/ol.2020.11290.
    12. Kawai, S. et al. (2020). Three-dimensional culture models mimic colon cancer heterogeneity induced by different microenvironments. Sci Rep. 10(1):3156. doi: 10.1038/s41598-020-60145-9.
    13. Kisin, E. R. et al. (2020). Enhanced morphological transformation of human lung epithelial cells by continuous exposure to cellulose nanocrystals. Chemosphere. doi: 10.1016/j.chemosphere.2020.126170.
    14. Queckbörner, S. et al. (2020). Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther. 11(1):15. doi: 10.1186/s13287-019-1496-2.
    15. Song, S. et al. (2019). Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45+CD19- cells but in the ALDHhigh Cells. J. Cancer. doi: 10.7150/jca.35000.
    16. Yang, B. et al. (2019). Stopping transformed cancer cell growth by rigidity sensing. Nat Mater. doi: 10.1038/s41563-019-0507-0.
    17. Speth, J.M. et al. (2019). Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics. JCI Insight. 4(20). pii: 131340. doi: 10.1172/jci.insight.131340.
    18. Kim, D. et al. (2019). Anticancer effect of XAV939 is observed by inhibiting lactose dehydrogenase A in a 3‑dimensional culture of colorectal cancer cells. Oncology Letters. doi: 10.3892/ol.2019.10813.
    19. Copeland, B.T. et al. (2019). Factors that influence the androgen receptor cistrome in benign and malignant prostate cells. Mol Oncol. doi: 10.1002/1878-0261.12572.
    20. Oliveira-Mateos, C. et al. (2019). The transcribed pseudogene RPSAP52 enhances the oncofetal HMGA2-IGF2BP2-RAS axis through LIN28B-dependent and independent let-7 inhibition. Nat Commun. 10(1):3979. doi: 10.1038/s41467-019-11910-6.
    21. Fukuchi, H. et al. (2019). Forkhead box B2 inhibits the malignant characteristics of the pancreatic cancer cell line Panc-1 in vitro. Genes Cells. doi: 10.1111/gtc.12717.
    22. Salgia, M.M. et al. (2019). Different roles of peroxisome proliferator-activated receptor gamma isoforms in prostate cancer. Am J Clin Exp Urol. 7(3):98-109.   
    23. Sceberras, V. et al. (2019). Preclinical study for treatment of hypospadias by advanced therapy medicinal products. World J Urol. doi: 10.1007/s00345-019-02864-x.
    24. Eckerdt, F. et al. (2019). Potent Antineoplastic Effects of Combined PI3Kα-MNK Inhibition in Medulloblastoma. Mol Cancer Res. doi: 10.1158/1541-7786.MCR-18-1193.
    25. Zhao, H. et al. (2019). The Effect of Endothelial Cells on UVB-induced DNA Damage and Transformation of Keratinocytes In 3D Polycaprolactone Scaffold Co-culture System. Photochem Photobiol. 95(1):338-344. doi: 10.1111/php.13006.
    26. He, C. et al. (2019). YAP1-LATS2 feedback loop dictates senescent or malignant cell fate to maintain tissue homeostasis. EMBO Rep. 20(3). pii: e44948. doi: 10.15252/embr.201744948.
    27. Ito, E. et al. (2019). Tumorigenicity assay essential for facilitating safety studies of hiPSC-derived cardiomyocytes for clinical application. Sci Rep. 9(1):1881. doi: 10.1038/s41598-018-38325-5.
    28. Bunda, S. et al. (2019). CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun. 10(1):661. doi: 10.1038/s41467-018-08087-9.
    29. Gökmen-Polar, Y. et al. (2019). Splicing factor ESRP1 controls ER-positive breast cancer by altering metabolic pathways. EMBO Rep. 20(2). pii: e46078. doi: 10.15252/embr.201846078.
    30. Koh, B. et al. (2019). Effect of fibroblast co-culture on the proliferation, viability and drug response of colon cancer cells. Oncol Lett. 17(2):2409-2417. doi: 10.3892/ol.2018.9836.
CBA-135
CBA-140		 　
    1. Buranarom, A. et al. (2021). Dichloromethane increases mutagenic DNA damage and transformation ability in cholangiocytes and enhances metastatic potential in cholangiocarcinoma cell lines. Chem Biol Interact. doi: 10.1016/j.cbi.2021.109580 (#CBA-135).
    2. Nehme, Z. et al. (2021). Polyploid giant cancer cells, stemness and epithelial-mesenchymal plasticity elicited by human cytomegalovirus. Oncogene. doi: 10.1038/s41388-021-01715-7 (#CBA-135).
    3. Andrade, F. et al. (2021). Polymeric micelles targeted against CD44v6 receptor increase niclosamide efficacy against colorectal cancer stem cells and reduce circulating tumor cells in vivo. J Control Release. 331:198-212. doi: 10.1016/j.jconrel.2021.01.022 (#CBA-135).
    4. Wakae, K. et al. (2020). EBV-LMP1 induces APOBEC3s and mitochondrial DNA hypermutation in nasopharyngeal cancer. Cancer Med. doi: 10.1002/cam4.3357 (#CBA-135).
    5. Lv, W. et al. (2020). Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features. Sci Bull. doi: 10.1016/j.scib.2020.03.040 (#CBA-135).
    6. Murata, M. et al. (2020). OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma. J Clin Med. 9(3). pii: E618. doi: 10.3390/jcm9030618 (#CBA-135).
    7. Hernandez, D.M. et al. (2020). IPF pathogenesis is dependent upon TGFβ induction of IGF-1. FASEB J. doi: 10.1096/fj.201901719RR (#CBA-135).
    8. Sand, A. et al. (2019). WEE1 inhibitor, AZD1775, overcomes trastuzumab resistance by targeting cancer stem-like properties in HER2-positive breast cancer. Cancer Lett. 472:119-131. doi: 10.1016/j.canlet.2019.12.023 (#CBA-135).
    9. Lim, S. et al. (2019). Targeting the interaction of AIMP2-DX2 with HSP70 suppresses cancer development. Nat Chem Biol. 16(1):31-41. doi: 10.1038/s41589-019-0415-2 (#CBA-135).
    10. Ha, Y. et al. (2019). Induction of Lysosome‐associated Protein Transmembrane 4 Beta via Sulfatase 2 Enhances Autophagic Flux in Liver Cancer Cells. Hepatol Commun. doi: 10.1002/hep4.1429 (#CBA-135).
    11. Mawaribuchi, S, et al. (2019). The rBC2LCN-positive subpopulation of PC-3 cells exhibits cancer stem-like properties. Biochem Biophys Res Commun. pii: S0006-291X(19)30994-5. doi: 10.1016/j.bbrc.2019.05.108 (#CBA-135).
    12. Oushy, S. et al. (2018). Glioblastoma multiforme-derived extracellular vesicles drive normal astrocytes towards a tumour-enhancing phenotype. Philos Trans R Soc Lond B Biol Sci. 373(1737). pii: 20160477. doi: 10.1098/rstb.2016.0477 (#CBA-135).
    13. Kumar, A. et al. (2018). The Human Cytomegalovirus Strain DB Activates Oncogenic Pathways in Mammary Epithelial Cells. EBioMedicine. 30:167-183. doi: 10.1016/j.ebiom.2018.03.015 (#CBA-135).
    14. van der Toorn, M. et al. (2018). The biological effects of long-term exposure of human bronchial epithelial cells to total particulate matter from a candidate modified-risk tobacco product. Toxicol In Vitro. 50:95-108. doi: 10.1016/j.tiv.2018.02.019 (#CBA-140).
    15. Lim, S.K. et al. (2016). Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of YAP/TZA transcriptional coactivator WBP2. Cancer Res. 76:6278-6289 (#CBA-135).
    16. Kumar, A. et al. (2016). Tumor control by human cytomegalovirus in a murine model of hepatocellular carcinoma. Mol Ther. doi:10.1038/mto.2016.12 (#CBA-135).
    17. Montalbano, M. et al. (2016). Modeling of hepatocytes proliferation isolated from proximal and distal zones from human hepatocellular carcinoma lesion. PLoS One 11:e0153613 (#CBA-140).
    18. Mardin, B. R. et al. (2015). A cell-based model system links chromothripsis with hyperploidy. Mol Syst Biol. 11:828 (#CBA-135).
    19. Monot, M. et al. (2015). Early steps of Jaagsiekte sheep retrovirus-mediated cell transformation involve the interaction between env and the RALBP1 cellular protein. J Virol. 89:8462-8473 (#CBA-135).
    20. Bon, H. et al. (2015). Salt-inducible kinase 2 regulates mitotic progression and transcription in prostate cancer. Mol Cancer Res. 13:620-635 (#CBA-135).
    21. Fatemi, M. et al. (2014). Epigenetic silencing of CHD5, a novel tumor-suppressor gene, occurs in early colorectal cancer stages. Cancer. 120:172-180 (#CBA-135).
    22. Park, H. et al. (2014). Distinct roles of DKK1 and DKK2 in tumor angiogenesis.  Angiogenesis. 17:221-234 (#CBA-135).
    23. Wang, X. et al. (2014).  Commensal Bacteria Drive Endogenous Transformation and Tumour Stem Cell Marker Expression Through a Bystander Effect. Gut. 10.1136/gutjnl-2014-307213 (#CBA-135).
    24. Bottero, V. et al. (2013). Kaposi's Sarcoma-Associated Herpesvirus-Positive Primary Effusion Lymphoma Tumor Formation in NOD/SCID Mice Is Inhibited by Neomycin and Neamine Blocking Angiogenin's Nuclear Translocation. J. Virol. 87:11806-11820 (#CBA-135).
    25. Singh, R. et al. (2013). Increasing the Complexity of Chromatin: Functionally Distinct Roles for Replication-Dependent Histone H2A Isoforms in Cell Proliferation and Carcinogenesis. Nucleic Acids Res. 10.1093/nar/gkt736 (#CBA-135).
    26. Shukla, A. et al. (2013). Extracellular Signal–Regulated Kinase 5: A Potential Therapeutic Target for Malignant Mesotheliomas. Clin. Cancer Res. 19:2071-2083 (#CBA-135).
    27. Niccoli, S. et al. (2012).The Asian-American E6 Variant Protein of Human Papillomavirus 16 Alone Is Sufficient To Promote Immortalization, Transformation, and Migration of Primary Human Foreskin Keratinocytes. J. Virol. 86:12384-12396 (#CBA-135).
    28. Hong, S.W. et al. (2012). Ring Finger Protein 149 Is an E3 Ubiquitin Ligase Active on Wild-type v-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF). J. Biol. Chem. 287:24017-24025 (#CBA-135).
    29. Lee, H.J. et al. (2012). Chemokine (C-X-C Motif) Ligand 12 Is Associated with Gallbladder Carcinoma Progression and Is a Novel Independent Poor Prognostic Factor. Clin. Cancer. Res. 18:3270-3280 (#CBA-135). 
    30. Chapeau, E.A. et al.(2012).Ecotropic Viral Integration Site 1 (EVI1) Regulates Multiple Cellular Processes Important for Cancer and is a Synergistic Partner for FOS Protein in Invasive Tumors. Proc Natl Acad Sci 109:2168-2173.(#CBA-135)

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
CytoSelect™ 96-well Cell Transformation Assay CBA-130 96 assays CELL BIOLABS
CytoSelect™ 96-well Cell Transformation Assay CBA-130-5 5x96 assays CELL BIOLABS
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric CBA-135-5 5x96 assays CELL BIOLABS
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric CBA-135 96 assays CELL BIOLABS
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric CBA-140-5 5x96 assays CELL BIOLABS
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric CBA-140 96 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
CBA-130
Product
CytoSelect™ 96-well Cell Transformation Assay
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-130-5
Product
CytoSelect™ 96-well Cell Transformation Assay
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-135-5
Product
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-135
Product
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-140-5
Product
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-140
Product
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
Size
96 assays
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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