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제품 상세
AAV Helper Free Packaging System | AAV Helper Free Expression System 이미지

AAV Helper Free Packaging System | AAV Helper Free Expression System

AAV Helper Free System은 live helper adenovirus 필요없이 pHelper plasmid를 통해 AAV(adeno-associated virus)를 생산하는 시스템입니다. (AAV native serotypes 1 through 6, AAV-DJ and AAV-DJ/8)

프린트 제품 문의

특징

 

  • Live helper virus 을 제거함으로써 안전하고 편리한 gene delivery system 입니다.
  • AAV packaging을 위한 adenoviral genes 들은 pHelper plasmid (E2A, E4 and VA RNA)에 의해 제공되거나 293 packaging cells (E1)로부터 공급됩니다.
  • 1-6까지의 native serotypes 및 AAV-DJ 와 AAV-DJ/8 군으로 공급됩니다.
  • AAV-DJ 군은 8가지의 다른 native serotype (NAAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, caprine AAV)을 조합하여 생성된 hybrid capsid를 제공함으로써, 다양한 tissue 와 cell types에서 native serotypes보다 높은 infection 효율을 보입니다.
  • AAV-DJ/8군은 AAV-DJ의 heparin binding domain에 point mutation을 일으킨 제품으로, 이를 통해 생성된 recombinant AAV는 in vivo상에서 heart, brain tissue등에서 native serotypes 8 (AAV-8), 9 (AAV-9)와 유사하게 발현됩니다.
  • Expression vector, GFP control vector, pHelper Vector 만 제공되는 Packaging System 및 전용 expression vector가 함께 제공되는 Expression System 이 있습니다.



원리 

  • AAV Helper Free System은 helper AAV를 사용하지 않고, GOI를 포함하는 재조합 AAV를 생성합니다. AAV 팩키징에 필요한 adenovirus 유전자는 pHelper plasmid (E2A, E4, VA RNA) 또는 293 packaging cell (E1)에서 제공됩니다.




제품 구성 

 

ComponentsPackaging SystemExpression System
pAAV VectorYesYes
pHelper VectorYesYes
pAAV-GFP Control VectorYesYes
pAAV Expression Vector-Yes

 


Relative in vitro Infectivity of AAV Vectors

Cell Line AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6 AAV-8 AAV-9 AAV-DJ AAV-DJ/8
Huh-7 13 100 2.5 0 0.1 10 0.7 0 500 0.2
HEK293 25 100 2.5 0.1 0.1 5 0.7 0.1 500 0.3
HeLa 3 100 2 0.1 6.7 1 0.2 0.1 667 0.2
HepG2 3 100 16.7 0.3 1.7 5 0.3 ND 1250 0.5
Hep1A 20 100 0.2 1 0.1 1 0.2 0 400 0.1
911 17 100 11 0.2 0.1 17 0.1 ND 500 0
CHO 100 100 14 1.4 333 50 10 1 25000 5
COS 33 100 33 3.3 5 14 2 0.5 500 0.3
MeWo 10 100 20 0.3 6.7 10 1 0.2 2857 1
NIH3T3 10 100 2.9 2.9 0.3 10 0.3 ND 500 0.1
A549 14 100 20 ND 0.5 10 0.5 0.1 1000 0.1
HT1180 20 100 10 0.1 0.3 33 0.5 0.1 333 0.2
Monocytes 1111 100 ND ND 125 1429 ND ND 100 ND
Immature DC 2500 100 ND ND 222 2857 ND ND 200 ND
Mature DC 2222 100 ND ND 333 3333 ND ND 100 ND

Note: Infectivity rates normalized to AAV-2 = 100. ND = Not Determined

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
AAV-1 Helper Free Bicistronic Expression System (GFP) VPK-418-SER1 1 kit CELL BIOLABS
AAV-1 Helper Free Expression System VPK-410-SER1 1 kit CELL BIOLABS
AAV-1 Helper Free Packaging System VPK-401 1 kit CELL BIOLABS
AAV-1 Helper Free Promoterless Expression System VPK-411-SER1 1 kit CELL BIOLABS
AAV-2 Helper Free Bicistronic Expression System (GFP) VPK-418-SER2 1 kit CELL BIOLABS
AAV-2 Helper Free Expression System VPK-410-SER2 1 kit CELL BIOLABS
AAV-2 Helper Free Packaging System VPK-402 1 kit CELL BIOLABS
AAV-2 Helper Free Promoterless Expression System VPK-411-SER2 1 kit CELL BIOLABS
AAV-3 Helper Free Bicistronic Expression System (GFP) VPK-418-SER3 1 kit CELL BIOLABS
AAV-3 Helper Free Expression System VPK-410-SER3 1 kit CELL BIOLABS
AAV-3 Helper Free Packaging System VPK-403 1 kit CELL BIOLABS
AAV-3 Helper Free Promoterless Expression System VPK-411-SER3 1 kit CELL BIOLABS
AAV-4 Helper Free Bicistronic Expression System (GFP) VPK-418-SER4 1 kit CELL BIOLABS
AAV-4 Helper Free Expression System VPK-410-SER4 1 kit CELL BIOLABS
AAV-4 Helper Free Packaging System VPK-404 1 kit CELL BIOLABS
AAV-4 Helper Free Promoterless Expression System VPK-411-SER4 1 kit CELL BIOLABS
AAV-5 Helper Free Bicistronic Expression System (GFP) VPK-418-SER5 1 kit CELL BIOLABS
AAV-5 Helper Free Expression System VPK-410-SER5 1 kit CELL BIOLABS
AAV-5 Helper Free Packaging System VPK-405 1 kit CELL BIOLABS
AAV-5 Helper Free Promoterless Expression System VPK-411-SER5 1 kit CELL BIOLABS
AAV-6 Helper Free Bicistronic Expression System (GFP) VPK-418-SER6 1 kit CELL BIOLABS
AAV-6 Helper Free Expression System VPK-410-SER6 1 kit CELL BIOLABS
AAV-6 Helper Free Packaging System VPK-406 1 kit CELL BIOLABS
AAV-6 Helper Free Promoterless Expression System VPK-411-SER6 1 kit CELL BIOLABS
AAV-DJ Helper Free Bicistronic Expression System (GFP) VPK-418-DJ 1 kit CELL BIOLABS
AAV-DJ Helper Free Expression System VPK-410-DJ 1 kit CELL BIOLABS
AAV-DJ Helper Free Packaging System VPK-400-DJ 1 kit CELL BIOLABS
AAV-DJ Helper Free Promoterless Expression System VPK-411-DJ 1 kit CELL BIOLABS
AAV-DJ/8 Helper Free Bicistronic Expression System (GFP) VPK-418-DJ-8 1 kit CELL BIOLABS
AAV-DJ/8 Helper Free Expression System VPK-410-DJ-8 1 kit CELL BIOLABS
AAV-DJ/8 Helper Free Packaging System VPK-400-DJ-8 1 kit CELL BIOLABS
AAV-DJ/8 Helper Free Promoterless Expression System VPK-411-DJ-8 1 kit CELL BIOLABS
scAAV-1 Helper Free Expression System VPK-430-SER1 1 kit CELL BIOLABS
scAAV-2 Helper Free Expression System VPK-430-SER2 1 kit CELL BIOLABS
scAAV-3 Helper Free Expression System VPK-430-SER3 1 kit CELL BIOLABS
scAAV-4 Helper Free Expression System VPK-430-SER4 1 kit CELL BIOLABS
scAAV-5 Helper Free Expression System VPK-430-SER5 1 kit CELL BIOLABS
scAAV-6 Helper Free Expression System VPK-430-SER6 1 kit CELL BIOLABS
scAAV-DJ Helper Free Expression System VPK-430-DJ 1 kit CELL BIOLABS
scAAV-DJ/8 Helper Free Expression System VPK-430-DJ-8 1 kit CELL BIOLABS
전체보기
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER1
Product
AAV-1 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER1
Product
AAV-1 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-401
Product
AAV-1 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER1
Product
AAV-1 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER2
Product
AAV-2 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER2
Product
AAV-2 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-402
Product
AAV-2 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER2
Product
AAV-2 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER3
Product
AAV-3 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER3
Product
AAV-3 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-403
Product
AAV-3 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER3
Product
AAV-3 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER4
Product
AAV-4 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER4
Product
AAV-4 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-404
Product
AAV-4 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER4
Product
AAV-4 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER5
Product
AAV-5 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER5
Product
AAV-5 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-405
Product
AAV-5 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER5
Product
AAV-5 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-SER6
Product
AAV-6 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-SER6
Product
AAV-6 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-406
Product
AAV-6 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-SER6
Product
AAV-6 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-DJ
Product
AAV-DJ Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-DJ
Product
AAV-DJ Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-400-DJ
Product
AAV-DJ Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-DJ
Product
AAV-DJ Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-418-DJ-8
Product
AAV-DJ/8 Helper Free Bicistronic Expression System (GFP)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-410-DJ-8
Product
AAV-DJ/8 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-400-DJ-8
Product
AAV-DJ/8 Helper Free Packaging System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-411-DJ-8
Product
AAV-DJ/8 Helper Free Promoterless Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER1
Product
scAAV-1 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER2
Product
scAAV-2 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER3
Product
scAAV-3 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER4
Product
scAAV-4 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER5
Product
scAAV-5 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-SER6
Product
scAAV-6 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-DJ
Product
scAAV-DJ Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-430-DJ-8
Product
scAAV-DJ/8 Helper Free Expression System
Size
1 kit
Qty
Data Sheet
MSDS
전체보기

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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