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AAV 정제 | AAV Purification Kits 이미지

AAV 정제 | AAV Purification Kits

ViraBind™ AAV Purification Kit 는 초원심분리를 하지 않고, single-step purification matrix와 centrifugal concentrator를 이용하여 Adeno-Associated Virus (AAV)를 신속하고 간편하게 정제 및 농축합니다.

프린트 제품 문의

ViraBind™ AAV Purification Mega Kit (#VPK-141)

 

  • 초원심분리를 하지 않고, single-step purification matrix와 column을 이용하여 바이러스 상층액에서 1차 정제 후, centrifugal concentrator로 추가 정제/농축 합니다. 
  • 3시간이 소요되며, 높은 회수율 (>60%)을 제공합니다. 
  • 유전자 전달에 가장 많이 이용되는 일반적인 AAV-2와 in vitro에서 감염율이 높은 AAV-DJ의 정제/농축에 효과적입니다. 
  • 구성 : 
    1. ViraBind™ AAV Reagent A
    2. ViraBind™ AAV Reagent B
    3. AAV Purification Matrix
    4. Purification Columns
    5. Purification Buffer
    6. Elution Buffer
    7. Centrifugal Concentrators

 

  

  

 

  

Purification of AAV2-GFP. AAV2-GFP was produced by a helper-free system in 293 cells. AAV supernatant was subjected to the purification steps. Samples from each fraction were used to infect 293 cells, GFP positive cells were scored by counting after three days. A: AAV Supernatant; B: Flow through; C: 1st wash; D: Elution. 

 

 

 

 

ViraBind™ AAV Purification Kit (#VPK-140) 

 

  • 초원심분리를 하지 않고, single-step purification matrix를 이용하여 바이러스 상층액에서 1차 정제 후, centrifugal concentrator로 추가 정제/농축 합니다. 
  • 3시간이 소요되며, 높은 회수율 (>60%)을 제공합니다. 
  • 유전자 전달에 가장 많이 이용되는 일반적인 AAV-2와 in vitro에서 감염율이 높은 AAV-DJ의 정제/농축에 효과적입니다. 
  • 구성 : 
    1. ViraBind™ AAV Reagent A
    2. ViraBind™ AAV Reagent B
    3. AAV Purification Matrix
    4. Purification Buffer
    5. Elution Buffer
    6. Centrifugal Concentrators

 


 

 

사용 논문 

 

VPK-140 　
    1. Andari, J.E. et al. (2020). Production, Processing and Characterization of synthetic AAV Gene Therapy Vectors. Biotechnol J. doi: 10.1002/biot.202000025.
    2. Lau, J.M.H. et al. (2020). The role of neuronal excitability, allocation to an engram and memory linking in the behavioral generation of a false memory in mice. Neurobiol Learn Mem.  doi: 10.1016/j.nlm.2020.107284.
    3. Zhang, H. et al. (2020). Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem. pii: jbc.RA119.011487. doi: 10.1074/jbc.RA119.011487.
    4. Xu, Y. et al. (2020). Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway. Biomed Pharmacother. 125:109920. doi: 10.1016/j.biopha.2020.109920.
    5. Muheim, C.M. et al. (2019). Dynamic- and Frequency-Specific Regulation of Sleep Oscillations by Cortical Potassium Channels. Curr Biol. pii: S0960-9822(19)30936-4. doi: 10.1016/j.cub.2019.07.056.
    6. Han, Y.H. et al. (2019). A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis. J Clin Invest. 130:1684-1698. doi: 10.1172/JCI124219.
    7. Gao, J. et al. (2019). Viral Vector-Based Delivery of CRISPR/Cas9 and Donor DNA for Homology-Directed Repair in an In Vitro Model for Canine Hemophilia B. Mol Ther Nucleic Acids. 14:364-376. doi: 10.1016/j.omtn.2018.12.008.
    8. Tseng, S.J. et al. (2018). Targeting Tumor Microenvironment by Bioreduction-Activated Nanoparticles for Light-Triggered Virotherapy. ACS Nano. 12(10):9894-9902. doi: 10.1021/acsnano.8b02813.
    9. Ikegami, R. et al. (2018). Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity. Cell Rep. 24(11):2827-2837.e5. doi: 10.1016/j.celrep.2018.08.024.
    10. Fois, G. et al. (2018). ATP is stored in lamellar bodies to activate vesicular P2X4 in an autocrine fashion upon exocytosis. J Gen Physiol. 150(2):277-291. doi: 10.1085/jgp.201711870.
    11. Cohen, J.L. et al. (2017). miR-101a-3p and Ezh2 modulate anxiety-like behavior in high-responder rats. Eur J Neurosci. 46(7): 2241–2252.
    12. Mao, Q. et al. (2017). Role of dorsal root ganglion K2p1.1 in peripheral nerve injury-induced neuropathic pain. Mol Pain. 1744806917701135. doi: 10.1177/1744806917701135.
    13. Tsuneoka, Y. et al. (2015). Distinct preoptic-BST nuclei dissociate paternal and infanticidal behavior in mice. EMBO J.doi:10.15252/embj.201591942.
    14. Nishikawa, T. et al. (2015). Resetting the transcription factor network reverses terminal chronic hepatic failure. J Clin Invest.  doi: 10.1172/JCI73137.
    15. Stankowska, D. L. et al. (2015). Neuroprotective effects of transcription factor brn3b in an ocular hypertension rat model of glaucoma. Invest Ophthalmol Vis Sci.  doi: 10.1167/iovs.14-15008.
    16. Inaba, Y. et al. (2014). Gadd34 regulates liver regeneration in hepatic steatosis. Hepatology. 6:1343-1356.
    17. Moshiri, F. et al. (2014). Inhibiting the Oncogenic mir-221 by microRNA Sponge: Toward microRNA-Based Therapeutics for Hepatocellular Carcinoma. Gastroenterol Hepatol Bed Bench. 7:43-54. 
    18. Inaba, Y. et al. (2014). Gadd34 regulates liver regeneration in hepatic steatosis. Hepatology. doi: 10.1002/hep.27619.
    19. Rodriguez, J.P. et al. (2014). Abrogation of β-Catenin Signaling in Oligodendrocyte Precursor Cells Reduces Glial Scarring and Promotes Axon Regeneration after CNS Injury. J Neurosci. 34:10285-10297.
    20. Uchida, S. et al. (2010). Early Life Stress Enhances Behavioral Vulnerability to Stress through the Activation of REST4-Mediated Gene Transcription in the Medial Prefrontal Cortex of Rodents. J. Neurosci. 30:15007-15018.
VPK-141 　
    1. Liu, Z. et al. (2021). AGEs exacerbates coronary microvascular dysfunction in NoCAD by activating endoplasmic reticulum stress- mediated PERK signaling pathway. Metabolism. doi: 10.1016/j.metabol.2021.154710.
    2. Zeng, J. et al (2020). The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis. Cell Stem Cell. S1934-5909(20)30350-7. doi: 10.1016/j.stem.2020.07.012.
    3. Liu, Z. et al. (2020). Diabetes mellitus exacerbates post-myocardial infarction heart failure by reducing sarcolipin promoter methylation. ESC Heart Fail. doi: 10.1002/ehf2.12789.
    4. Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.
    5. Nazari, M. et al. (2014). AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation. BMC Biotechnol. 14:87

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
ViraBind™ AAV Purification Kit VPK-140 10 preps CELL BIOLABS
ViraBind™ AAV Purification Mega Kit VPK-141-5 10 preps CELL BIOLABS
ViraBind™ AAV Purification Mega Kit VPK-141 2 preps CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
VPK-140
Product
ViraBind™ AAV Purification Kit
Size
10 preps
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-141-5
Product
ViraBind™ AAV Purification Mega Kit
Size
10 preps
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-141
Product
ViraBind™ AAV Purification Mega Kit
Size
2 preps
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
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