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AAV 정량 | AAV Quantitation & Titration 이미지

AAV 정량 | AAV Quantitation & Titration

QuickTiter AAV Quantitation Kit는 Purified AAV 및 unpurified AAV supernatant에서 viral nucleic acid 함량 측정을 위해 이용되며, QuickTiter AAV Titer ELISA Kit는 viral capsid protein 양 측정에 이용됩니다.

프린트 제품 문의

QuickTiter™ AAV Quantitation Kit (#VPK-145) 

 

  • Purified AAV 및 unpurified AAV supernatant의 viral nucleic acid 함량을 측정합니다. 
  • Detection sensitivity :
    - 1x10^9 GC/ml (genome copy/ml) for unpurified AAV-2, AAV-DJ
    - 5x10^10 GC/ml for purified AAV sample from any serotype
  • 구성 :
    1. ViraBind™ AAV Reagent A
    2. ViraBind™ AAV Reagent B
    3. QuickTiter™ AAV Capture Matrix
    4. QuickTiter™ AAV Wash Solution
    5. QuickTiter™ Solution C
    6. CyQuant® GR Dye
    7. QuickTiter™ AAV DNA Standard 

 

 

AAV-2 DNA Standard Curve. The QuickTiter™ AAV-2 DNA Standard was diluted as described in the Assay Protocol. Fluorescence measurement was performed on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.

 

 

 

AAV Titer ELISA Kit (#VPK-5146)

 

  • purified AAV viral sample에서 viral capsid protein의 양을 측정하는 ELISA kit 입니다.
  • 적용 serotype : AAV-1, AAV-2, AAV-3, AAV-5, AAV-6, AAV-8, AAV-9, AAV-10, AAV-DJ, AAV-DJ/8

 

 

 

 

사용 논문

 

VPK-145 　
    1. Wahis, J. et al. (2021). Astrocytes mediate the effect of oxytocin in the central amygdala on neuronal activity and affective states in rodents. Nat Neurosci. doi: 10.1038/s41593-021-00800-0.
    2. Francisco, J. et al. (2021). AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis. Sci Rep. 11(1):10553. doi: 10.1038/s41598-021-89989-5.
    3. Kwon, O.C. et al. (2021). SGK1 inhibition in glia ameliorates pathologies and symptoms in Parkinson disease animal models. EMBO Mol Med. doi: 10.15252/emmm.202013076.
    4. Lebeau, P.F. et al. (2020). The loss-of-function PCSK9Q152H variant increases ER chaperones GRP78 and GRP94 and protects against liver injury. J Clin Invest. doi: 10.1172/JCI128650.
    5. Kawaguchi, Y. et al. (2020). Endoplasmic reticulum chaperone BiP/GRP78 knockdown leads to autophagy and cell death of arginine vasopressin neurons in mice. Sci Rep. 10(1):19730. doi: 10.1038/s41598-020-76839-z.
    6. Rai, R. et al. (2020). Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome. Nat Commun. 11(1):4034. doi: 10.1038/s41467-020-17626-2.
    7. Zeng, J. et al (2020). The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis. Cell Stem Cell. S1934-5909(20)30350-7. doi: 10.1016/j.stem.2020.07.012.
    8. Tang, Y. et al. (2020). Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons. Nat Neurosci. doi: 10.1038/s41593-020-0674-y.
    9. Zhu, J. et al. (2020). Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells. Bio-protocol. 10(07): e3573. doi: 10.21769/BioProtoc.3573.
    10. Wu, Z. et al. (2020). Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 11(1):1105. doi: 10.1038/s41467-020-14855-3.
    11. Zhang, H. et al. (2020). Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem. pii: jbc.RA119.011487. doi: 10.1074/jbc.RA119.011487.
    12. Xu, Y. et al. (2020). Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway. Biomed Pharmacother. 125:109920. doi: 10.1016/j.biopha.2020.109920.
    13. Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.
    14. Niranjan, N. et al. (2019). Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy. Am J Physiol Cell Physiol. doi: 10.1152/ajpcell.00146.2019.
    15. Ferretti, V. et al. (2019). Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice. Curr Biol. pii: S0960-9822(19)30499-3. doi: 10.1016/j.cub.2019.04.070.
    16. Hasan, M.T. et al. (2019). A Fear Memory Engram and Its Plasticity in the Hypothalamic Oxytocin System. Neuron. pii: S0896-6273(19)30386-1. doi: 10.1016/j.neuron.2019.04.029.
    17. Lee, S. et al. (2019). Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo. Acta Biomater. pii: S1742-7061(19)30287-9. doi: 10.1016/j.actbio.2019.04.044.
    18. Nakamura, M. et al. (2019). Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Cell Metab. pii: S1550-4131(19)30005-1. doi: 10.1016/j.cmet.2019.01.005.
    19. Tseng, S.J. et al. (2018). Targeting Tumor Microenvironment by Bioreduction-Activated Nanoparticles for Light-Triggered Virotherapy. ACS Nano. 12(10):9894-9902. doi: 10.1021/acsnano.8b02813.
    20. Ikegami, R. et al. (2018). Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity. Cell Rep. 24(11):2827-2837.e5. doi: 10.1016/j.celrep.2018.08.024.
    21. Menon, R. et al. (2018). Oxytocin Signaling in the Lateral Septum Prevents Social Fear during Lactation. Curr Biol. 28(7):1066-1078.e6. doi: 10.1016/j.cub.2018.02.044.
    22. Wen, L. et al. (2018). Transient High Pressure in Pancreatic Ducts Promotes Inflammation and Alters Tight Junctions via Calcineurin Signaling in Mice. Gastroenterology. 155(4):1250-1263.e5. doi: 10.1053/j.gastro.2018.06.036.
    23. McGrady NR, et al. (2017). Upregulation of the endothelin A (ETA) receptor and its association with neurodegeneration in a rodent model of glaucoma. BMC Neurosci. 18(1):27. doi: 10.1186/s12868-017-0346-3.
    24. Wang, P., et al. (2017). Tau interactome mapping based identification of Otub1 as Tau deubiquitinase involved in accumulation of pathological Tau forms in vitro and in vivo. Acta Neuropathol. doi:10.1007/s00401-016-1663-9.
    25. Tseng, S.J. et al. (2016). Remote control of light-triggered virotherapy. ACS Nano 10:10339-10346.
    26. Eliava, M. et al. (2016). A new population of parvocellular oxytocin neurons controlling magnocellular neuron activity and inflammatory pain processing. Neuron 89:1291-1304.
    27. Madroñal, N. et al. (2016). Rapid erasure of hippocampal memory following inhibition of dentate gyrus granule cells. Nat Commun. doi:10.1038/ncomms10923.
    28. Park, H. Y. et al. (2016). Gadd45β ameliorates L-DOPA-induced dyskinesia in a Parkinson's disease mouse model. Neurobiol Dis. 89:169-179.
    29. Tran, L. & Keele, N. B. (2016). CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala. Behav Brain Res. 303:152-159.
    30. Chen, W. et al. (2016). The silencing of cathepsin K used in gene therapy for periodontal disease reveals the role of cathepsin K in chronic infection and inflammation. J Periodontal Res.doi:10.1111/jre.12345.

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
QuickTiter™ AAV Quantitation Kit VPK-145 20 assays CELL BIOLABS
QuickTiter™ AAV Titer ELISA Kit VPK-5146-5 5 x 96 assays CELL BIOLABS
QuickTiter™ AAV Titer ELISA Kit VPK-5146 96 assays CELL BIOLABS
AAV Rep ELISA Kit VPK-5118 96 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
VPK-145
Product
QuickTiter™ AAV Quantitation Kit
Size
20 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-5146-5
Product
QuickTiter™ AAV Titer ELISA Kit
Size
5 x 96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-5146
Product
QuickTiter™ AAV Titer ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-5118
Product
AAV Rep ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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