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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

특징  


    • Parental HEK293 cell에서 유래된 cell line으로, virus가 잘 도입되도록 평평한 형태와 높은 부착성을 제공합니다.
    • 종류 
      • AAV : 293AAV Cell Line (#AAV-100)
      • Adenovirus : 293AD Cell Line (#AD-100)
      • Lentivirus : 293LTV Cell Line (#LTV-100)
      • Retrovirus : 293RTV Cell Line (#RV-100)

     

     

     

     

    사용 논문

     

    AAV-100 　
      1. Yun, T. et al. (2021). Inhibitor of DNA binding 2 (Id2) mediates microtubule polymerization in the brain by regulating αK40 acetylation of α-tubulin. Cell Death Discov. 7(1):257. doi: 10.1038/s41420-021-00652-4.
      2. Chao, G. et al. (2021). Measurement of Large Serine Integrase Enzymatic Characteristics in HEK293 Cells Reveals Variability and Influence on Downstream Reporter Expression. FEBS J. doi: 10.1111/febs.16037.
      3. Sveidahl Johansen, O. et al. (2021). Lipolysis drives expression of the constitutively active receptor GPR3 to induce adipose thermogenesis. Cell. doi: 10.1016/j.cell.2021.04.037.
      4. Hu, J. et al. (2021). Co-opting regulation bypass repair as a gene correction strategy for monogenic diseases. Mol Ther. doi: 10.1016/j.ymthe.2021.04.017.
      5. Motori, E. et al. (2020). Larsson, Neuronal metabolic rewiring promotes resilience to neurodegeneration caused by mitochondrial dysfunction. Sci. Adv. 6(35). doi: 10.1126/sciadv.aba8271.
      6. Gӧbel, J. et al. (2020). Mitochondria-Endoplasmic Reticulum Contacts in Reactive Astrocytes Promote Vascular Remodeling. Cell Metab. pii: S1550-4131(20)30120-0. doi: 10.1016/j.cmet.2020.03.005.
      7. Lin, Y.H. et al. (2020). Accumbal D2R-medium spiny neurons regulate aversive behaviors through PKA-Rap1 pathway. Neurochem Int. doi: 10.1016/j.neuint.2020.104935.
      8. Siegrist, C.M. et al. (2020). CRISPR/Cas9 as an antiviral against Orthopoxviruses using an AAV vector. Sci Rep. 10(1):19307. doi: 10.1038/s41598-020-76449-9.
      9. Sun, W. et al. (2020). snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis. Nature. 587(7832):98-102. doi: 10.1038/s41586-020-2856-x.
      10. Bougioukli, S. et al. (2020). Limited potential of AAV-mediated gene therapy in transducing human mesenchymal stem cells for bone repair applications. Gene Ther. doi: 10.1038/s41434-020-0182-4.
      11. Chen, S.H. et al. (2020). A Simple, Two-Step, Small-Scale Purification of Recombinant Adeno-Associated Viruses. J Virol Methods. doi: 10.1016/j.jviromet.2020.113863.
      12. Wu, Z. et al. (2020). Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 11(1):1105. doi: 10.1038/s41467-020-14855-3.
      13. Miyawaki, T. et al. (2020). Visualization and molecular characterization of whole-brain vascular networks with capillary resolution. Nat Commun. 11(1):1104. doi: 10.1038/s41467-020-14786-z.
      14. Funahashi, Y. et al. (2019). Phosphorylation of Npas4 by MAPK Regulates Reward-Related Gene Expression and Behaviors. Cell Rep. 29(10):3235-3252.e9. doi: 10.1016/j.celrep.2019.10.116.
      15. Nakano, R. et al . (2019). Auxin-mediated rapid degradation of target proteins in hippocampal neurons. Neuroreport. 30(13):908-913. doi: 10.1097/WNR.0000000000001299.
      16. Kuzumaki, N. et al. (2019). Cell-specific overexpression of COMT in dopaminergic neurons of Parkinson’s disease. Brain. doi: 10.1093/brain/awz084.
      17. Ao, H. et al. (2019). Egr1 mediates retinal vascular dysfunction in diabetes mellitus via promoting p53 transcription. J Cell Mol Med. doi: 10.1111/jcmm.14225.
      18. Nakamura, M. et al. (2019). Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Cell Metab. pii: S1550-4131(19)30005-1. doi: 10.1016/j.cmet.2019.01.005.
      19. Iwasawa, C. et al. (2019). Regional and temporal regulation and role of somatostatin receptor subtypes in the mouse brain following systemic kainate-induced acute seizures. Neurosci Res. pii: S0168-0102(18)30670-9. doi: 10.1016/j.neures.2019.01.004.
      20. Hashimoto, H. et al. (2016). Study on AAV-mediated gene therapy for diabetes in humanized liver mouse to predict efficacy in humans. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2016.08.104.
      21. Lee, S. E. et al. (2016). nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology. Proc Natl Acad Sci U S A. doi:10.1073/pnas.1600944113.
      22. Lutz, D. et al. (2014).  Myelin Basic Protein Cleaves Cell Adhesion Molecule L1 and Promotes Neuritogenesis and Cell Survival. J Biol Chem. 289:13503-13518.
    AD-100 　
      1. Wade, M. et al. (2021). In vivo generation of collagen specific Tregs with AAV8 suppresses autoimmune responses and arthritis in DBA1 mice through IL10 production. Sci Rep. 11(1):18204. doi: 10.1038/s41598-021-97739-w.
      2. Light, T.P. et al. (2021). A Cancer Mutation Promotes EphA4 Oligomerization and Signaling by Altering the Conformation of the SAM Domain. J Biol Chem. doi: 10.1016/j.jbc.2021.100876.
      3. Koduri, V. et al. (2021). Targeting oncoproteins with a positive selection assay for protein degraders. Sci Adv. 7(6):eabd6263. doi: 10.1126/sciadv.abd6263.
      4. Tanaka, N. et al. (2020). Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning. Biotechniques. doi: 10.2144/btn-2020-0052.
      5. Gehring, M.P. et al. (2020). Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains. Cell Signal. doi: 10.1016/j.cellsig.2020.109668.
      6. Cho, J.H. et al. (2020). CD9 induces cellular senescence and aggravates atherosclerotic plaque formation. Cell Death Differ. doi: 10.1038/s41418-020-0537-9.
      7. Hu, Z. (2020). Tissue factor as a new target for CAR-NK cell immunotherapy of triple-negative breast cancer. Sci Rep. 10(1):2815. doi: 10.1038/s41598-020-59736-3.
      8. Watanabe, H. et al. (2018). Sirt2 facilitates hepatic glucose uptake by deacetylating glucokinase regulatory protein. Nat Commun. 9(1):30. doi: 10.1038/s41467-017-02537-6.
      9. Wu, B. et al. (2018). Glutaminase 1 regulates the release of extracellular vesicles during neuroinflammation through key metabolic intermediate alpha-ketoglutarate. J Neuroinflammation. 15(1):79. doi: 10.1186/s12974-018-1120-x.
      10. Dai, C.Y. et al. (2018). The IL-6/STAT3 pathway upregulates microRNA-125b expression in hepatitis C virus infection. Oncotarget. 9(13):11291-11302. doi: 10.18632/oncotarget.24129.
      11. Lin, G.Z. et al. (2018). Immunogenicity of recombinant Adenovirus co-expressing the L7/L12 and BCSP31 proteins of Brucella abortus. Kafkas Univ Vet Fak Derg. 24 (2): 211-217. doi: 10.9775/kvfd.2017.18644.
      12. Zaric, M. et al. (2017). Long-lived tissue resident HIV-1 specific memory CD8+ T cells are generated by skin immunization with live virus vectored microneedle arrays. J Control Release. 268:166-175. doi: 10.1016/j.jconrel.2017.10.026.
      13. Ravindran, D. et al. (2017). Chemokine binding protein 'M3' limits atherosclerosis in apolipoprotein E-/- mice. PLoS One. 12(3):e0173224. doi: 10.1371/journal.pone.0173224.
      14. Ridiandries, A. et al. (2017). Broad-Spectrum Inhibition of the CC-Chemokine Class Improves Wound Healing and Wound Angiogenesis. Int J Mol Sci. doi: 10.3390/ijms18010155.
      15. Bae, E. J. et al. (2015). Cell models to study cell-to-cell transmission of α-synuclein. Methods Mol Biol. 1345:291-298.
      16. Strathearn, K. E. & Pardo, A. M. P. (2015). Parameters to Consider When Expanding Cells on Corning® Microcarriers. Corning Application Note.
      17. Sugiyama, K. et al. (2014). Expression of the miR200 family of microRNAs in mesothelial cells suppresses the dissemination of ovarian cancer cells. Mol Cancer Ther. 13:2081-2091.
      18. Peng, D. et al. (2014). Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. Gut 63:540-551.
      19. Peng, D. et al. (2011). Glutathione Peroxidase 7 Protects Against Oxidative DNA Damage in Oesophageal Cells. Gut. 61:1250-1260.
      20. Kothari, H. el at. (2010) Cystine 186-cystine 209 disulfide bond is not essential for the procoagulant activity of tissue factor or for its de-encryption. Blood 115:4273-4283.
      21. Fang, S. et al. (2007). Coordinated recruitment of histone methyltransferase G9a and other chromatin modifying enzymes in SHP-mediated regulation of hepatic bile acid metabolism. Mol. Cell. Biol. 27:1407-1424.
      22. Ponugoti, B. et al. (2007). Functional interaction of HNF-4 and PGC-1alpha in CYP7A1 regulation is inhibited by a key lipogenic activator, SREBP-1c. Mol. Endocrinol. 21:2698-2712.
    LTV-100 　
      1. Wade, M. et al. (2021). In vivo generation of collagen specific Tregs with AAV8 suppresses autoimmune responses and arthritis in DBA1 mice through IL10 production. Sci Rep. 11(1):18204. doi: 10.1038/s41598-021-97739-w.
      2. Smith, C.M. et al. (2021). Phosphatidylserine binding directly regulates TIM-3 function. Biochem J. 478(17):3331-3349. doi: 10.1042/BCJ20210425.
      3. Kiss, E. et al. (2021). Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model. Mol Cell Neurosci. doi: 10.1016/j.mcn.2021.103624.
      4. Kiss, E. et al. (2021). Artemisinin-treatment in pre-symptomatic APP-PS1 mice increases gephyrin phosphorylation at Ser270: a modification regulating postsynaptic GABAAR density. Biol Chem. doi: 10.1515/hsz-2021-0153.
      5. Jeong, J. et al. (2021). Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts. J Anim Sci Biotechnol. 12(1):64. doi: 10.1186/s40104-021-00583-1.
      6. Martin-Jaular, L. et al. (2021). Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J. doi: 10.15252/embj.2020105492.
      7. Xie, W. et al. (2021). Pterostilbene accelerates wound healing by modulating diabetes-induced estrogen receptor β suppression in hematopoietic stem cells. Burns Trauma. doi: 10.1093/burnst/tkaa045.
      8. Sinha, A. et al. (2020). RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational Status. Cancers (Basel). 12(11):E3178. doi: 10.3390/cancers12113178.
      9. Sun, W. et al. (2020). snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis. Nature. 587(7832):98-102. doi: 10.1038/s41586-020-2856-x.
      10. Yoshihara, E. et al. (2020). Immune-evasive human islet-like organoids ameliorate diabetes. Nature. doi: 10.1038/s41586-020-2631-z.
      11. Baci, D. et al. (2020). Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells. Cells. 9(6):E1527. doi: 10.3390/cells9061527.
      12. Smolková, K. et al. (2020). SIRT3 and GCN5L regulation of NADP+- and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2. Sci Rep. 10(1):8677. doi: 10.1038/s41598-020-65351-z.
      13. Lucchini, F.C. et al. (2020). ASK1 inhibits browning of white adipose tissue in obesity. Nat Commun. 11(1):1642. doi: 10.1038/s41467-020-15483-7.
      14. Hsu, W.L. et al. (2020). Restoring Wnt6 signaling ameliorates behavioral deficits in MeCP2 T158A mouse model of Rett syndrome. Sci Rep. 10(1):1074. doi: 10.1038/s41598-020-57745-w.
      15. Harten, I.A. et al. (2020). The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation. Matrix Biol. pii: S0945-053X(20)30008-1. doi: 10.1016/j.matbio.2020.01.002.
      16. Yanai, M. et al. (2019). ADAR2 is involved in self and nonself recognition of Borna disease virus genomic RNA in the nucleus. J Virol. pii: JVI.01513-19. doi: 10.1128/JVI.01513-19.
      17. Rubio, R.M. et al. (2019). Inhibition of ULK1 and Beclin1 by an α-herpesvirus Akt-like Ser/Thr kinase limits autophagy to stimulate virus replication. Proc Natl Acad Sci U S A. pii: 201915139. doi: 10.1073/pnas.1915139116.
      18. Madan, E. et al. (2019). HIF-transcribed p53 chaperones HIF-1α. Nucleic Acids Res. pii: gkz766. doi: 10.1093/nar/gkz766.
      19. Zhou, Y. et al. (2019). Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac Reprogramming. Cell Stem Cell. pii: S1934-5909(19)30224-3. doi: 10.1016/j.stem.2019.05.020.
      20. Kobayashi, M. et al. (2019). AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke. Cell Rep. 28(4):979-991.e6. doi: 10.1016/j.celrep.2019.06.075.
      21. Peng, D. et al. (2019). NRF2 antioxidant response protects against acidic bile salts-induced oxidative stress and DNA damage in esophageal cells. Cancer Lett. pii: S0304-3835(19)30326-X. doi: 10.1016/j.canlet.2019.05.031.
      22. Lau, M.T. et al. (2019). Molecular dissection of box jellyfish venom cytotoxicity highlights an effective venom antidote. Nat Commun. 10(1):1655. doi: 10.1038/s41467-019-09681-1.
      23. Bhattacharya, S. et al. (2019). Lentiviral-mediated delivery of classical swine fever virus Erns gene into porcine kidney-15 cells for production of recombinant ELISA diagnostic antigen. Mol Biol Rep. doi: 10.1007/s11033-019-04829-0.
      24. Navinés-Ferrer, A. et al. (2018). MRGPRX2-mediated mast cell response to drugs used in perioperative procedures and anaesthesia. Sci Rep. 8(1):11628. doi: 10.1038/s41598-018-29965-8.
      25. Meier, S. et al. (2018). Non-invasive detection of adeno-associated viral gene transfer using a genetically encoded CEST-MRI reporter gene in the murine heart. Sci Rep. 8(1):4638. doi: 10.1038/s41598-018-22993-4.
      26. Ghosh, N. & Banerjee, E.R. (2017). A Review on Various Tissue Engineering Techniques to Induce Differentiation of Pluripotent Stem Cells. Medical Glory. 1(2):130-149.
      27. Agudelo, D. et al. (2017). Marker-free coselection for CRISPR-driven genome editing in human cells. Nat Methods. 14(6):615–620. doi:10.1038/nmeth.4265.
      28. Zheng, L. et al. (2017). Lym-1 Chimeric Antigen Receptor T Cells Exhibit Potent Anti-Tumor Effects against B-Cell Lymphoma. Int J Mol Sci. 18(12). pii: E2773. doi: 10.3390/ijms18122773.
      29. Hsu, W.L. et al. (2017). Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2. BMC Biol. 15(1):112. doi: 10.1186/s12915-017-0452-9.
      30. Tao, C.C. et al. (2017). Epigenetic regulation of HDAC1 SUMOylation as an endogenous neuroprotection against Aβ toxicity in a mouse model of Alzheimer's disease. Cell Death Differ. doi: 10.1038/cdd.2016.161.

     

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    		 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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