ProteoSEC Dynamic Size Exclusion Columns | 고해상도 단백질 정제 컬럼

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Category 전체보기: 전기영동 > 단백질 전기영동 전기영동 > 헥산 전기영동

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ProteoSEC Dynamic Size Exclusion Columns | 고해상도 단백질 정제 컬럼

Maker : PROTEIN ARK

ProteoSEC Dynamic Size Exclusion Columns은 글로벌 표준인 GE(Cytiva)의 Superdex™ 시리즈와 대등한 성능을 갖춘 고해상도 단백질 정제용 컬럼입니다.

프린트 제품 문의

특징

Superdex™ 200 pg 및 75 pg와 대등한 분리능을 갖춘 35 µm 미세 입자 기반의 고해상도 컬럼으로, 1 kDa부터 5,000 kDa까지 폭넓은 단백질을 정교하게 다듬는(Polishing) 최적의 솔루션입니다.
모든 컬럼은 Theoretical Plate Number (>10,000 N/m)와 피크 비대칭도(0.7-1.6) 테스트를 거쳐 고해상도 분리 성능을 보증합니다.
Dextran과 아가로스의 고성능 공중합체(Co-polymer)를 사용하여, 평균 ca. 35 µm의 미세하고 균일한 입자 크기를 유지하여 날카로운 피크 분해능을 구현합니다.
모든 수용성 버퍼와 호환되며, 실온 및 저온실(4°C) 환경에서 안정적으로 작동합니다.
단백질 정제의 최종 단계인 High-definition polishing(순도 극대화 및 응집체 제거)에 최적화되어 있습니다.

종류

단백질 크기에 따라 선택 가능한 4가지 HR(High Resolution) 옵션을 제공합니다.

1-10 HR: 1~10 kDa (펩타이드 및 초소형 분자)
3-70 HR: 3~70 kDa (소형 단백질, Superdex 75 pg급)
6-600 HR: 6~600 kDa (범용 단백질 정제, Superdex 200 pg급)
6-5000 HR: 6~5,000 kDa (거대 단백질 복합체 및 바이러스 입자)

11/30, 16/60, 26/60 규격을 모두 지원하여 연구 규모에 맞춰 선택 가능합니다.

11/30
16/60 (GE 16/600 사이즈와 동일)
26/60 (GE 26/600 사이즈와 동일)

GE Superdex와 호환되는 모델은 다음과 같습니다.

6-600kDa (GE Superdex S200 pg 대체)
3-70kDa (GE Superdex S75 pg 대체)

Dimension	Resolution	MW	Cat. No.	Product
11/30 (11mm ID x 30cm Bed)	1-10 HR	1-10 kDa	SEC-D-11/30-1-10	ProteoSEC Dynamic 11/30 1-10 HR SEC Column: 11 mm ID x 30 cm Bed Length; 1-10 kDA HR Resin
	3-70 HR	3-10 kDa	SEC-D-11/30-3-70	ProteoSEC Dynamic 11/30 3-70 HR SEC Column: 11 mm ID x 30 cm Bed Length; 3-70 kDA HR Resin
	6-600 HR	6-600 kDa	SEC-D-11/30-6-600	ProteoSEC Dynamic 11/30 6-600 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-600 kDA HR Resin
	6-5000 HR	6-5000 kDa	SEC-D-11/30-6-5000	ProteoSEC Dynamic 11/30 6-5000 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-5000 kDA HR Resin
16/60 (16mm ID x 60cm Bed)	1-10 HR	1-10 kDa	SEC-D-16/60-1-10	ProteoSEC Dynamic 16/60 1-10 HR SEC Column: 16 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin
	3-70 HR	3-10 kDa	SEC-D-16/60-3-70	ProteoSEC Dynamic 16/60 3-70 HR SEC Column: 16 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin
	6-600 HR	6-600 kDa	SEC-D-16/60-6-600	ProteoSEC Dynamic 16/60 6-600 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin
	6-5000 HR	6-5000 kDa	SEC-D-16/60-6-5000	ProteoSEC Dynamic 16/60 6-5000 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin
26/60 (26mm ID x 60cm Bed)	1-10 HR	1-10 kDa	SEC-D-26/60-1-10	ProteoSEC Dynamic 26/60 1-10 HR SEC Column: 26 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin
	3-70 HR	3-10 kDa	SEC-D-26/60-3-70	ProteoSEC Dynamic 26/60 3-70 HR SEC Column: 26 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin
	6-600 HR	6-600 kDa	SEC-D-26/60-6-600	ProteoSEC Dynamic 26/60 6-600 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin
	6-5000 HR	6-5000 kDa	SEC-D-26/60-6-5000	ProteoSEC Dynamic 26/60 6-5000 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin

사양

Matrix	Highly cross-linked polysaccharide composite of Dextran and Agarose
Particle size (um)	35um (in the range of 20 – 50um)
Column body max pressure	6 Bar, 0.6 MPa (3-70 HR) 5 Bar, 0.5 MPa (6-600 HR) 3 Bar, 0.3 Mpa (6-5000 HR)
Operating flow velocity	0.15 - 0.8 ml/min (11/30 Columns) 0.30 - 1.6 ml/min (16/60 Columns) 0.80 - 4.4 ml/min (26/60 Columns)
pH stability	2-14 (short term) and 3-12 (long term)
Working temperature	+4 °C to +30 °C
Chemical stability	All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 20% ethanol (Concentration of alcohol should not exceed 30% v/v)
Avoid	Oxidizing agents
Storage	0.02% sodium azide or 20% ethanol

데이터

Chromatographic separation and calibration curve the ProteoSEC 16/600 6-600 HR column

Figure 1: Separation of test substances on a ProteoSEC 16/600 6-600 HR gel filtration column.

Flowrate: 1 ml/min; sample loading 0.5 ml; mobile phase: PBS (phosphate buffered saline); Model proteins 1: Aprotinin (Mr 6,500); 2: Cytochrome c (Mr 12,300); 3: -Lactoglobulin (Mr 35,000); 4: BSA (Mr 67,000); 5: Globulin IgG (Mr 158,000); 6: Apoferritin (Mr 440,000); 7: Thyroglobulin (Mr 669,000)

Chromatographic separation and calibration curve the ProteoSEC 16/600 3 – 70 HR column

Figure 2: Separation of test substances on a ProteoSEC 16/600 3-70 HR gel filtration column.

Flowrate: 1 ml/min; sample loading: 0.5 ml; mobile phase: PBS (phosphate buffered saline); Model proteins 1: Vit B-12 (Mr 1,200); 2: Aprotinin (Mr 6,500); 3: Cytochrome c (Mr 12,300); 4: Lactoglobulin (Mr 35,000); 5: BSA (Mr 67,000).

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
ProteoSEC Dynamic 11/30 1-10 HR SEC Column: 11 mm ID x 30 cm Bed Length; 1-10 kDA HR Resin	SEC-D-11/30-1-10	1 PK	PROTEIN ARK
ProteoSEC Dynamic 11/30 3-70 HR SEC Column: 11 mm ID x 30 cm Bed Length; 3-70 kDA HR Resin	SEC-D-11/30-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic 11/30 6-5000 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-5000 kDA HR Resin	SEC-D-11/30-6-5000	1 PK	PROTEIN ARK
ProteoSEC Dynamic 11/30 6-600 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-600 kDA HR Resin	SEC-D-11/30-6-600	1 PK	PROTEIN ARK
ProteoSEC Dynamic 16/60 1-10 HR SEC Column: 16 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin	SEC-D-16/60-1-10	1 PK	PROTEIN ARK
ProteoSEC Dynamic 16/60 3-70 HR SEC Column: 16 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin	SEC-D-16/60-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic 16/60 6-5000 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin	SEC-D-16/60-6-5000	1 PK	PROTEIN ARK
ProteoSEC Dynamic 16/60 6-600 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin	SEC-D-16/60-6-600	1 PK	PROTEIN ARK
ProteoSEC Dynamic 26/60 1-10 HR SEC Column: 26 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin	SEC-D-26/60-1-10	1 PK	PROTEIN ARK
ProteoSEC Dynamic 26/60 3-70 HR SEC Column: 26 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin	SEC-D-26/60-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic 26/60 6-5000 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin	SEC-D-26/60-6-5000	1 PK	PROTEIN ARK
ProteoSEC Dynamic 26/60 6-600 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin	SEC-D-26/60-6-600	1 PK	PROTEIN ARK

전체보기

1
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-1-10
Product	ProteoSEC Dynamic 11/30 1-10 HR SEC Column: 11 mm ID x 30 cm Bed Length; 1-10 kDA HR Resin
Size	1 PK
Qty

2
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-3-70
Product	ProteoSEC Dynamic 11/30 3-70 HR SEC Column: 11 mm ID x 30 cm Bed Length; 3-70 kDA HR Resin
Size	1 PK
Qty

3
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-6-5000
Product	ProteoSEC Dynamic 11/30 6-5000 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-5000 kDA HR Resin
Size	1 PK
Qty

4
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-6-600
Product	ProteoSEC Dynamic 11/30 6-600 HR SEC Column: 11 mm ID x 30 cm Bed Length; 6-600 kDA HR Resin
Size	1 PK
Qty

5
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-1-10
Product	ProteoSEC Dynamic 16/60 1-10 HR SEC Column: 16 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin
Size	1 PK
Qty

6
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-3-70
Product	ProteoSEC Dynamic 16/60 3-70 HR SEC Column: 16 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin
Size	1 PK
Qty

7
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-6-5000
Product	ProteoSEC Dynamic 16/60 6-5000 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin
Size	1 PK
Qty

8
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-6-600
Product	ProteoSEC Dynamic 16/60 6-600 HR SEC Column: 16 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin
Size	1 PK
Qty

9
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-1-10
Product	ProteoSEC Dynamic 26/60 1-10 HR SEC Column: 26 mm ID x 60 cm Bed Length; 1-10 kDA HR Resin
Size	1 PK
Qty

10
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-3-70
Product	ProteoSEC Dynamic 26/60 3-70 HR SEC Column: 26 mm ID x 60 cm Bed Length; 3-70 kDA HR Resin
Size	1 PK
Qty

11
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-6-5000
Product	ProteoSEC Dynamic 26/60 6-5000 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-5000 kDA HR Resin
Size	1 PK
Qty

12
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-6-600
Product	ProteoSEC Dynamic 26/60 6-600 HR SEC Column: 26 mm ID x 60 cm Bed Length; 6-600 kDA HR Resin
Size	1 PK
Qty