단백질 정제 컬럼 | ProteoSEC HPLC Column

메뉴 바로가기 본문 바로가기

고마바이오텍(주)

로그인이 필요합니다.

Category 전체보기: 전기영동 > 단백질 전기영동 전기영동 > 헥산 전기영동

Cat. No.전체보기: 검색 결과가 없습니다.

Product전체보기: 검색 결과가 없습니다.

Brand전체보기: 검색 결과가 없습니다.

제품 상세

단백질 정제 컬럼 | ProteoSEC HPLC Column

ProteoSEC HPLC Column (=ProteoSEC Dynamic Size Exclusion Column)은 단백질 정제용 HPLC column입니다.

프린트 제품 문의

특징

High resolution (HR) preparative grade column으로 GE Healthcare의 Superdex 제품을 대체할 수 있습니다.
High molecule도 overlap 없이 잘 분리됩니다.
각 column은 Height Equivalent to a Theoretical Plate (HETP)와 peak assymmetry 인증을 받았습니다. (Theoretical Plate Number: > 10,000 N/m & Peak Asymmetry: 0.7-1.5)
High Resolution (HR) resin은 dextran과 agarose의 co-polymer이며 particle의 평균크기는 35um (20-50um) 입니다.
16/60 HR column은 non-adjustable end adaptor (classic design)와 adjustable end adaptor (dynamic design) 모두 가능합니다.
3가지 사이즈
1) 11/30
2) 16/60 (GE 16/600 사이즈와 동일)
3) 26/60 (GE 26/600 사이즈와 동일)
2가지 분리 범위 : 6-600kDa (GE Superdex S200 pg 대체), 3-70kDa (GE Superdex S75 pg 대체)

종류

	ProteoSEC 3-70 HR	ProteoSEC 6-600 HR
Separation range	3-70 kDa	6-600 kDa
Column dimensions	11mm ID x 30cm column length (28.5ml CV) 16mm ID x 60cm column length (120ml CV) 26mm ID x 60cm column length (320ml CV)
Particle size	20-50um (35um avg.)
Format supplied	Pre-packed 11/30, 16/60, 26/60

사양

Matrix : Highly cross-linked polysaccharide composite of Dextran and Agarose
Particle size (um) : 35um (in the range of 20 – 50 m)
Column body max pressure : 6 Bar, 0.6 MPa (3-70 Columns) & 5 Bar, 0.5 MPa (6-600 Columns)
Operating flow velocity :

0.15 – 0.8 ml/min (11/30 Columns)
0.30 – 1.6 ml/min (16/60 Columns)
0.80 – 4.4 ml/min (26/60 Columns)

pH stability : 2 – 14 (short term) and 3 – 12 (long term)
Working temperature : +4 °C to +30 °C
Chemical stability : All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 20% ethanol (Concentration of alcohol should not exceed 30% v/v)
Avoid : Oxidizing agents
Storage : 0.02% sodium azide or 20% ethanol

데이터

Chromatographic separation and calibration curve the ProteoSEC 16/600 6-600 HR column

Figure 1: Separation of test substances on a ProteoSEC 16/600 6-600 HR gel filtration column.

Flowrate: 1 ml/min; sample loading 0.5 ml; mobile phase: PBS (phosphate buffered saline); Model proteins 1: Aprotinin (Mr 6,500); 2: Cytochrome c (Mr 12,300); 3: -Lactoglobulin (Mr 35,000); 4: BSA (Mr 67,000); 5: Globulin IgG (Mr 158,000); 6: Apoferritin (Mr 440,000); 7: Thyroglobulin (Mr 669,000)

Chromatographic separation and calibration curve the ProteoSEC 16/600 3 – 70 HR column

Figure 2: Separation of test substances on a ProteoSEC 16/600 3-70 HR gel filtration column.

Flowrate: 1 ml/min; sample loading: 0.5 ml; mobile phase: PBS (phosphate buffered saline); Model proteins 1: Vit B-12 (Mr 1,200); 2: Aprotinin (Mr 6,500); 3: Cytochrome c (Mr 12,300); 4: Lactoglobulin (Mr 35,000); 5: BSA (Mr 67,000).

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
ProteoSEC Dynamic Size Exclusion Column (11 mm ID; 30 cm length, 3-70 kDa HR resin)	SEC-D-11/30-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic Size Exclusion Column (11 mm ID; 30 cm length, 6-600 kDa HR resin)	SEC-D-11/30-6-600	1 PK	PROTEIN ARK
ProteoSEC Dynamic Size Exclusion Column (16 mm ID; 60 cm length, 3-70 kDa HR resin)	SEC-D-16/60-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic Size Exclusion Column (16 mm ID; 60 cm length, 6-600 kDa HR resin)	SEC-D-16/60-6-600	1 PK	PROTEIN ARK
ProteoSEC Dynamic Size Exclusion Column (26 mm ID; 60 cm length, 3-70 kDa HR resin)	SEC-D-26/60-3-70	1 PK	PROTEIN ARK
ProteoSEC Dynamic Size Exclusion Column (26 mm ID; 60 cm length, 6-600 kDa HR resin)	SEC-D-26/60-6-600	1 PK	PROTEIN ARK

1
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-3-70
Product	ProteoSEC Dynamic Size Exclusion Column (11 mm ID; 30 cm length, 3-70 kDa HR resin)
Size	1 PK
Qty

2
Maker	PROTEIN ARK
Cat.No.	SEC-D-11/30-6-600
Product	ProteoSEC Dynamic Size Exclusion Column (11 mm ID; 30 cm length, 6-600 kDa HR resin)
Size	1 PK
Qty

3
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-3-70
Product	ProteoSEC Dynamic Size Exclusion Column (16 mm ID; 60 cm length, 3-70 kDa HR resin)
Size	1 PK
Qty

4
Maker	PROTEIN ARK
Cat.No.	SEC-D-16/60-6-600
Product	ProteoSEC Dynamic Size Exclusion Column (16 mm ID; 60 cm length, 6-600 kDa HR resin)
Size	1 PK
Qty

5
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-3-70
Product	ProteoSEC Dynamic Size Exclusion Column (26 mm ID; 60 cm length, 3-70 kDa HR resin)
Size	1 PK
Qty

6
Maker	PROTEIN ARK
Cat.No.	SEC-D-26/60-6-600
Product	ProteoSEC Dynamic Size Exclusion Column (26 mm ID; 60 cm length, 6-600 kDa HR resin)
Size	1 PK
Qty