제품 상세 Antibody 6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며, 최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다. 프린트 제품 문의 특징 Motor Neuron Progenitor cell은 Motor neuron cell fate marker인 OLIG2와 SOX1를 발현합니다.배양 14일차부터는 motor neuron의 대표적인 마커인 HB9, LIM3, OLIG2 및 TUJ1을 발현하고, 14일이 더 경과한 이후에는 ChAT와 ISL1을 발현합니다.Motor Neuron Maturation Accelerator Supplement(#ax0179)는 운동 뉴런의 발생 환경을 재현하여 운동 뉴런이 빠르게 성숙하게 하여, 10일 내에 synchronized bursting을 발현하고, neuraxis가 발달되며, B9, ChAT2 및 Islet-1이 발현되도록 합니다. 동물모델을 대신하여 drug-screening assay, 운동신경세포 퇴행성 모델등의 연구에 이용됩니다.사양 Starting material Fibroblasts Donor gender Male Donor age at sampling 74 yrs Karyotype Normal Reprogramming method Episomal vector Induction method Monolayer & chemically defined medium Genetic modification None Size 1 vial (≥2 million cells/ vial) Growth properties Adherent 제품 Cat. No. Product Format Conc. ax0078 Human iPSC-Derived Motor Neuron Progenitors (Healthy) ≥2 million cells/vial ax0073 Human iPSC-Derived Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension) ≥2 million cells/vial ax0074 Human iPSC-Derived Motor Neuron Progenitors (ALS (C9ORF72 extension)) ≥2 million cells/vial ax0072 Motor Neuron Maintenance Medium 200 mL ax139888 (20 µg) Recombinant Human Ciliary-Neurotrophic Factor (CNTF) 20 μg Lyophilized Powder ax139800 (10 µg) Recombinant Human Brain-Derived Neurotrophic Factor (BDNF) 10 μg Lyophilized Powder ax139855 (10 µg) Recombinant Human Glial-Derived Neurotrophic Factor (GDNF) 10 μg Lyophilized Powder ax0179 Motor Neuron Maturation Accelerator supplement 1 mL 참고 데이터Phase contrast images show the morphology of human iPSC-derived motor neuron progenitors upon recovery from thawing.Axol’s Human iPSC-Derived Motor Neuron Progenitors express cell fate markers.Whole cell patch clamp recordings from human iPSC-derived motor neurons reveal the electrical properties and functional connectivity of our motor neurons.Motor Neuron Accelerator Media Supplement (#ax0179)Axol의 Human iPSC-derived Motor Neuron Progenitor(ax0078, ax0073, 또는 ax0074) 제품과 사용할 수 있습니다.다음의 제품을 함께 이용합니다.Unlock (ax0044 ) : fully defined & gentle detachment buffer 25 mLRecombinant Human Brain-derived Neurotrophic Factor (BDNF) (ax139800)Human GDNF (ax139855)Recombinant Human Ciliary – Neurotrophic Factor (CNTF) (ax139888)Figure 1. After 10 days, motor neurons have formed characteristic tight cell clusters.Figure 2. After 10 days, motor neurons also show synchronized burst firingFigure 3. After 10 days, motor neurons express key markers, like HB9, ChAT2, and Islet 1관련 자료ProtocolUser Guide: Human iPSC-derived Motor Neuron ProgenitorsPublicationsSpijkers, X.M., Pasteuning-Vuhman, S., Dorleijn, J.C. et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11, 2080 (2021). https://doi.org/10.1038/s41598-021-81335-zFiskum Vegard, Sandvig Axel, Sandvig Ioanna 2021. Silencing of Activity During Hypoxia Improves Functional Outcomes in Motor Neuron Networks in vitro. Frontiers in Integrative Neuroscience 15 2021. DOI=10.3389/fnint.2021.792863 Rimington, R.P., Fleming, J.W., Capel, A.J. et al. Bioengineered model of the human motor unit with physiologically functional neuromuscular junctions. Sci Rep 11, 11695 (2021). https://doi.org/10.1038/s41598-021-91203-5 주문정보 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS 전체보기 추천제품 관련제품 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS 전체보기 자료 웨비나/Video