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제품 상세
AXOL 운동뉴런 | Motor Neuron Progenitor & Media 이미지

AXOL 운동뉴런 | Motor Neuron Progenitor & Media

Human iPSC-Derived Motor Neuron은 Motor neuron cell fate markers (OLIG2과 SOX1)를 발현합니다. 배양 14일 후에 HB9, LIM3, OLIG2 and TUJ1을 발현하며 14일 이후에는 choline acetyltransferase (ChAT), islet-1 (ISL1)을 발현합니다.

프린트 제품 문의

특징

 

  • Motor Neuron Progenitor cell은 Motor neuron cell fate marker인 OLIG2와 SOX1를 발현합니다.
  • 배양 14일차부터는 motor neuron의 대표적인 마커인 HB9, LIM3, OLIG2 및 TUJ1을 발현하고, 14일이 더 경과한 이후에는 ChAT와 ISL1을 발현합니다.
  • Motor Neuron Maturation Accelerator Supplement(#ax0179)는 운동 뉴런의 발생 환경을 재현하여 운동 뉴런이 빠르게 성숙하게 하여,  10일 내에 synchronized bursting을 발현하고, neuraxis가 발달되며, B9, ChAT2 및 Islet-1이 발현되도록 합니다. 
  • 동물모델을 대신하여 drug-screening assay, 운동신경세포 퇴행성 모델등의 연구에 이용됩니다.


사양

Starting material Fibroblasts
Donor gender Male
Donor age at sampling 74 yrs
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size 1 vial (≥2 million cells/ vial)
Growth properties Adherent


제품

Cat. No. Product Name Format Conc.
ax0078 Human iPSC-Derived Motor Neuron Progenitors (Healthy) ≥2 million cells/vial
ax0073 Human iPSC-Derived Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension) ≥2 million cells/vial
ax0074 Human iPSC-Derived Motor Neuron Progenitors (ALS (C9ORF72 extension)) ≥2 million cells/vial
ax0072 Motor Neuron Maintenance Medium 200 mL
ax139888  Recombinant Human Ciliary-Neurotrophic Factor (CNTF) 20 μg Lyophilized Powder
ax139800 Recombinant Human Brain-Derived Neurotrophic Factor (BDNF) 10 μg Lyophilized Powder
ax139855 Recombinant Human Glial-Derived Neurotrophic Factor (GDNF) 10 μg Lyophilized Powder
ax0179 Motor Neuron Maturation Accelerator supplement 1 mL
ax0178 Human iPSC-derived Motor Neuron Progenitor Kit Human iPSC-derived Motor Neuron Progenitors (ax0078)
Motor Neuron Maintenance Medium (ax0072)
Motor Neuron Maturation Accelerator supplement (ax0179)
CNTF (ax139888)
BDNF (ax139800)
GDNF (ax139855)




참고 데이터

Phase contrast images show the morphology of human iPSC-derived motor neuron progenitors upon recovery from thawing.




Axol’s Human iPSC-Derived Motor Neuron Progenitors express cell fate markers.




Whole cell patch clamp recordings from human iPSC-derived motor neurons reveal the electrical properties and functional connectivity of our motor neurons.





Motor Neuron Accelerator Media Supplement (#ax0179)

  • Axol의 Human iPSC-derived Motor Neuron Progenitor(ax0078, ax0073, 또는 ax0074) 제품과 사용할 수 있습니다.
  • 다음의 제품을 함께 이용합니다.
    • Unlock (ax0044 ) : fully defined & gentle detachment buffer 25 mL
    • Recombinant Human Brain-derived Neurotrophic Factor (BDNF) (ax139800)
    • Human GDNF (ax139855)
    • Recombinant Human Ciliary – Neurotrophic Factor (CNTF) (ax139888)



Figure 1. After 10 days, motor neurons have formed characteristic tight cell clusters.



Figure 2. After 10 days, motor neurons also show synchronized burst firing





Figure 3. After 10 days, motor neurons express key markers, like HB9, ChAT2, and Islet 1


관련 자료

Protocol

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Human iPSC-derived Motor Neuron Progenitor Kit (male) ax0178 1 vial AXOL
Human iPSC-Derived Motor Neuron Progenitors ax0078 1 vial AXOL
Human iPSC-Derived Motor Neuron Progenitors (C9ORF72 expansion) ax0073 1 vial AXOL
Human iPSC-Derived Motor Neuron Progenitors- Amyotrophic Lateral Sclerosis (ALS) (C9ORF72 expansion) ax0074 1 vial AXOL
Motor Neuron Maintenance Medium ax0072 200 ml AXOL
Motor Neuron Maturation Accelerator supplement ax0179 1mL AXOL
Human beta-NGF ax139789 (20 ug) 20 ug AXOL
Human CNTF ax139888 (5 ug) 5 ug AXOL
Human CNTF ax139888 (20 ug) 20 ug AXOL
Human GDNF ax139855 (10 ug) 10 ug AXOL
Human/Murine/Rat BDNF ax139800 (10 ug) 10 ug AXOL
전체보기
Maker
AXOL
Cat.No.
ax0178
Product
Human iPSC-derived Motor Neuron Progenitor Kit (male)
Size
1 vial
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax0078
Product
Human iPSC-Derived Motor Neuron Progenitors
Size
1 vial
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax0073
Product
Human iPSC-Derived Motor Neuron Progenitors (C9ORF72 expansion)
Size
1 vial
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax0074
Product
Human iPSC-Derived Motor Neuron Progenitors- Amyotrophic Lateral Sclerosis (ALS) (C9ORF72 expansion)
Size
1 vial
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax0072
Product
Motor Neuron Maintenance Medium
Size
200 ml
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax0179
Product
Motor Neuron Maturation Accelerator supplement
Size
1mL
Qty
Data Sheet
Maker
AXOL
Cat.No.
ax139789 (20 ug)
Product
Human beta-NGF
Size
20 ug
Qty
Maker
AXOL
Cat.No.
ax139888 (5 ug)
Product
Human CNTF
Size
5 ug
Qty
Maker
AXOL
Cat.No.
ax139888 (20 ug)
Product
Human CNTF
Size
20 ug
Qty
Maker
AXOL
Cat.No.
ax139855 (10 ug)
Product
Human GDNF
Size
10 ug
Qty
Maker
AXOL
Cat.No.
ax139800 (10 ug)
Product
Human/Murine/Rat BDNF
Size
10 ug
Qty
전체보기

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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Data Sheet
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