EMBER™ Ultra RNA Gel Kit | 고마바이오텍

Five-fold dilutions of ssRNA Ladder from NEB (200 ng to 12.5 ng per lane) were separated on a 1% agarose/1X TBE gel using the EMBER™ Ultra RNA Gel Kit. The gel was imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator, the Safe Imager™ 2.0 Blue-Light Transilluminator (Thermo Fisher), or a UV transilluminator with SYBR® Filter. They EMBER™ Ultra gel system is optimized for Gel-Bright™ Laser Diode and blue light illuminators, but also can be used with UV illuminators with lower sensitivity.

Comparison of EMBER™ Ultra RNA Gel (left) with 1% agarose gel post-stained with SYBR® Gold. NEB ssRNA Ladder or total Jurkat cell RNA were denatured at 70°C for 10 minutes in EMBER™ Ultra RNA Loading Dye (for EMBER™ gel) or NEB RNA Loading Dye (for SYBR® Gel) and run on 1% agarose/1X TBE gels. EMBER® Ultra Gel was imaged immediately after electrophoresis, while the other gel was post-stained in 1X SYBR® Gold/1X TBE for 30 minutes. Gels were imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator. The EMBER Ultra® RNA Gel Kit offers comparable or superior sensitivity and resolution compared to SYBR® Gold, without a time-consuming post-staining step.

The ExoBrite™ EV Total RNA Isolation Kit was used to extract RNA from 6 x 10^10 EVs (derived from Jurkat cells, isolated by SEC) and a total yield of 100 ng RNA was obtained. 10 ng of the EV RNA was analyzed by gel electrophoresis using the EMBER™ Ultra RNA Gel Kit with a 1.5% gel. The gel was imaged using the Gel-Bright™ Laser Diode Gel Imager. L = Low Range ssRNA Ladder (NEB).