엑소좀 RNA 추출 | ExoBrite™ EV Total RNA Isolation Kit | 고마바이오텍

특징

• 정제된 EVs로부터 total RNA를 추출할 수 있습니다.
• 1×10^10 SEC 농축된 EVs에서 약 10-20 ng의 RNA를 추출할 수 있습니다.
• qPCR 또는 RNAseq와 같은 downstream application에 호환 가능합니다.
• 간단한 column-based 정제 방법입니다.
• phenol/chloroform 또는 에탄올 precipitation 단계를 진행하지 않습니다. 

구성 

• EV Lysis Buffer
• Column Binding Buffer
• DNase Buffer
• DNase I (lyophilized)
• Column Wash Buffer
• Spin Columns

The ExoBrite™ EV Total RNA Isolation Kit was used to extract RNA from 6 x 10^10 EVs (derived from Jurkat cells, isolated by SEC) and a total yield of 100 ng RNA was obtained. 10 ng of the EV RNA was analyzed by gel electrophoresis using the EMBER™ Ultra RNA Gel Kit with a 1.5% gel. The gel was imaged using the Gel-Bright™ Laser Diode Gel Imager. L = Low Range ssRNA Ladder (NEB).