EMBER™ Ultra DNA Gel Kit | 고마바이오텍

Agarose gel electrophoresis of 1 kb and 100 bp DNA ladders using the EMBER™ Ultra DNA Gel Kit compared to post-staining with SYBR® Gold. Five-fold serial dilutions of 1 kb DNA ladder (cat. no. 31080) or 100 bp DNA Ladder (cat. no. 31081) 1rom 200 ng to 0.8 ng per well were separated on 1% agarose/1X TBE gels. EMBER™ Ultra DNA Loading Buffer was used to load samples on EMBER™ Ultra Agarose, or 6X DNA Loading Buffer (Blue) was used to load samples on Agarose LE without fluorescent dye. The EMBER™ Ultra gel was imaged immediately after electrophoresis, while the other gel was stained for 30 minutes with 1X SYBR® Gold in 1X TBE and then imaged. Gels were imaged on a Safe Imager™ 2.0 Blue-Light Transilluminator. EMBER™ Ultra was roughly five times more sensitive than SYBR® Gold with improved band resolution, without the need for a time-consuming post-staining step. EMBER™ Ultra detected as little as 0.05 ng DNA (1.5 kb band in the 0.8 ng lane for 1 kb ladder).

Agarose gel electrophoresis of low molecular weight DNA using the EMBER™ Ultra DNA Gel Kit compared to post-staining with SYBR® Gold. Five-fold serial dilutions of GeneRuler™ Ultra Low Range (ULR) DNA Ladder (Thermo) or a 19 bp single stranded oligonucleotide from 200 ng to 0.8 ng per well were separated on 5% agarose/1X TBE gels. EMBER™ Ultra DNA Loading Buffer was used to load samples on EMBER™ Ultra Agarose, or 6X DNA Loading Buffer (Blue) was used to load samples on Agarose LE without fluorescent dye. The EMBER™ Ultra gel was imaged immediately after electrophoresis, while the other gel was stained for 30 minutes with 1X SYBR® Gold in 1X TBE and then imaged. Gels were imaged on a Safe Imager™ 2.0 Blue-Light Transilluminator (top) or UV transilluminator with SYBR® filter (bottom). EMBER™ Ultra was roughly five times more sensitive than SYBR® Gold with improved band resolution, without the need for a time-consuming post-staining step. EMBER™ Ultra detected as little as 0.05 ng DNA (300 bp band in the 0.08 ng lane).