바이러스 감염 titer 측정 | Virus Immunoplaque Assay | 고마바이오텍

Influenza A Immunoplaque Assay Kit (# VPK-5189)

• Anti-Influenza A Nucleoprotein antibody로 Influenza A virus로 감염된 세포의 plaque를 염색합니다. 
• 12well plate 기준으로 최대 60번 assay 할 수 있는 양으로 제공됩니다. 
• Detection : Immunocytochemistry
• Kit의 구성:

1. 5X DMEM Medium 
2. CytoSelect™ Agar Powder 
   
3. TPCK-Trypsin (100X) 
   
4. Anti-Influenza A Nucleoprotein Antibody (1000X) 
   
5. Secondary Antibody, HRP Conjugate (1000X)
   
6. DAB Substrate (25X) 
   
7. Diluent (10X) 
   

• 실험 결과

Figure 1: Plaque Formation in Influenza A Virus-Infected MDCK Cells. Influenza A virus was used to infect MDCK cells for 24 to 72 hrs. Post-infection 24 hrs, 48 hrs and 72 hrs, anti-Influenza A nucleoprotein immunostaining was performed as described in the Assay Protocol.