바이러스 감염 titer 측정 | Virus Immunoplaque Assay | 고마바이오텍

Influenza A Immunoplaque Assay Kit (# VPK-5189)

• Anti-Influenza A Nucleoprotein antibody로 Influenza A virus로 감염된 세포의 plaque를 염색합니다. 
• 12well plate 기준으로 최대 60번 assay 할 수 있는 양으로 제공됩니다. 
• Detection : Immunocytochemistry
• Kit의 구성:

1. 5X DMEM Medium 
2. CytoSelect™ Agar Powder 
   
3. TPCK-Trypsin (100X) 
   
4. Anti-Influenza A Nucleoprotein Antibody (1000X) 
   
5. Secondary Antibody, HRP Conjugate (1000X)
   
6. DAB Substrate (25X) 
   
7. Diluent (10X) 
   

• 실험 결과

Figure 1: Plaque Formation in Influenza A Virus-Infected MDCK Cells. Influenza A virus was used to infect MDCK cells for 24 to 72 hrs. Post-infection 24 hrs, 48 hrs and 72 hrs, anti-Influenza A nucleoprotein immunostaining was performed as described in the Assay Protocol.

Figure 2: Immunoplaque Assay Titration of Influenza A Viruses in MDCK Cells. Different dilutions of Influenza A Virus Culture Fluid were used to infect MDCK cells for 72 hrs. Anti-Influenza A nucleoprotein immunostaining (A) and Crystal Violet staining (B) were performed.

Influenza B Immunoplaque Assay Kit (#VPK-5190)

• Anti-Influenza B Nucleoprotein antibody로 Influenza B virus로 감염된 세포의 plaque를 염색합니다. 
• 12well plate 기준으로 최대 60번 assay 할 수 있는 양으로 제공됩니다. 
• Detection : Immunocytochemistry
• Kit의 구성:

1. 5X DMEM Medium 
2. CytoSelect™ Agar Powder
   
3. TPCK-Trypsin (100X) 
   
4. Anti-Influenza B Nucleoprotein Antibody (1000X) 
   
5. Secondary Antibody, HRP Conjugate (1000X)
   
6. DAB Substrate (25X) 
   
7. Diluent (10X) 
   

• 실험 결과

Figure 1: Plaque Formation in Influenza B Virus-Infected MDCK Cells. Influenza B virus was

used to infect MDCK cells for 24 to 72 hrs. Post-infection 24 hrs, 48 hrs and 72 hrs, anti-Influenza B 

nucleoprotein immunostaining was performed as described in the Assay Protocol.

Figure 2: Immunoplaque Assay Titration of Influenza B Viruses in MDCK Cells. Different dilutions of Influenza B Virus Culture Fluid were used to infect MDCK cells for 72 hrs. Anti-Influenza B nucleoprotein immunostaining (A) and Crystal Violet staining (B) were performed.

Viral Plaque Assay Kit (#VPK-5191)

• 전통적인 plaque 분석 방법을 이용하여 용해성 바이러스 (lytic virus)의 감염성을 측정합니다.
• 숙주 세포 단층에 바이러스를 희석하여 접종하여 감염시킨 후, agar 배지로 덮어 바이러스가 확산되지 않고 주변 세포만 감염시킬 수 있도록 한 뒤, 세포의 감염에 의해 생기는 플라크를 카운팅하는 방법입니다. 각각의 플라크는 감염성 바이러스 입자를 나타냅니다. 
  
• 12well plate 기준으로 최대 120번 assay 할 수 있는 양으로 제공됩니다. 
• Detection : Light Microscopy
• Kit의 구성:

1. CytoSelect™ Agar Powder 
2. 10X Fixation Solution
   
3. Cell Stain Solution 

• 실험 결과