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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

QuickTiter™ Adenovirus Quantitation Kit (#VPK-106) 

 

  • Purified adenovirus 및 Supernatant 에 존재하는 adenovirus를 정량합니다.
  • 형광 dye를 이용하여 염색 후 형광 plate를 판독합니다.
  • physical titer (VP/mL)가 충분할 경우 효과적이며, 한시간 이내에  결과를 얻을 수 있습니다. 
  • Limit of Detection: 10^10 viral particles/mL from 100 µL of adenoviral supernatant
  • 구성 :
    1. QuickTiter™ Solution A
    2. QuickTiter™ Adenovirus Capture Solution
    3. QuickTiter™ Solution B
    4. QuickTiter™ Solution C
    5. CyQuant® GR Dye
    6. QuickTiter™ Adenovirus DNA Standard


Adenovirus DNA Standard Curve. The QuickTiter™ Adenovirus DNA Standard was diluted as described in the above instructions. Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Device) with a 485/538 nm filter set and 530 nm cutoff.



QuickTiter™ Adenovirus Titer ELISA Kit (#VPK-110) 

 

  • Adenovirus capsid를 구성하는 단백질인 adenovirus hexon protein에 대한 항체를 이용하여 infected cell을 확인합니다. 
  • 일반적인 Plaque-forming unit assay보다 빠르고 정확한 측정 결과를 제공합니다.
  • Agar overlay 단계가 없어 사용이 편리합니다.
  • Adenovirus의 모든 serotype에 적용됩니다. 
  • 10일 이상 걸리는 고전적인 Plaque assay 방법에 비해, 분석 시간이 2.5일로 빠르게 단축됩니다.
  • Limit of Detection: 1x10^4 IFU/mL (10^6-10^7 VP/mL)
  • 구성 :
    1. Anti-Hexon Antibody
    2. Secondary Antibody, HRP Conjugate
    3. TMB Substrate
    4. Stop Solution
    5. Ad-β gal Positive Control


Ad-β gal Titration. Serial dilutions of the Ad-β gal positive control were used to infect HEK 293 cells for 48 hours. Left panel: Anti-Hexon ELISA was performed according to the assay protocol. 
Right panel: Adenovirus-infected 293 cells were visualized by anti-Hexon immunocytochemistry staining using QuickTiter™ Adenovirus Immunoassay Kit (#VPK-109). The picture was taken from 293 cells infected with 10^5 ifu/mL of Ad-β gal in a 12-well plate.



QuickTiter™ Adenovirus Titer Immunoassay (#VPK-109) 

  

  • Adenovirus hexon protein을 항체로 염색하여 infected cell을 확인합니다. 
  • 10일 이상 걸리는 고전적인 Plaque assay 방법에 비해, 분석 시간이 2.5일로 빠르게 단축됩니다.
  • Adenovirus의 모든 serotype에 적용됩니다. 
  • 구성  : 
    1. Anti-Hexon Antibody
    2. Secondary Antibody, HRP Conjugate
    3. DAB Substrate
    4. Diluent
    5. Ad-β gal Positive Control 



Ad-β gal Titration. Different dilutions of the Ad-β gal positive control were used to infect HEK 293 cells for 48 hrs. Anti-Hexon immunostaining was performed as described in Assay Instructions and X-gal staining is done by using β-Galactosidase Staining Kit (Cat. # AKR-100)



사용 논문

VPK-106 　
    1. Stone, A.V. et al. (2020). Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus. Cartilage. doi: 10.1177/1947603520958143. 
    2. Hossain, E. et al. (2020). Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism. Cancers (Basel). 12(5). pii: E1210. doi: 10.3390/cancers12051210.
    3. Habiba, U. et al. (2020). Cisplatin Relocalizes RNA Binding Protein HuR and Enhances the Oncolytic Activity of E4orf6 Deleted Adenovirus. Cancers. 12:809. doi: 10.3390/cancers12040809.
    4. Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.
    5. Yanagawa-Matsuda, A. et al. (2019). Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells. Oncol Rep. 41(2):954-960. doi: 10.3892/or.2018.6865.
    6. Yan, Y. et al. (2016). HIV-1 Vpr increases HCV replication through VprBP in cell culture. Virus Res. doi:10.1016/j.virusres.2016.07.007.
    7. Xie, Y. et al. (2016). A recombinant adenovirus expressing P12A and 3C protein of the type O foot-and-mouth disease virus stimulates systemic and mucosal immune responses in mice. BioMed Res Int. doi:10.1155/2016/7849203.
    8. Ran, L. et al. (2016). Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy. Biomaterials. 89:56-66.
    9. Nuche-Berenguer, B. et al. (2016). The p21-activated kinase, PAK2, is important in the activation of numerous pancreatic acinar cell signaling Cascades and in the onset of early pancreatitis events. Biochim Biophys Acta. doi:10.1016/j.bbadis.2016.02.008.
    10. Nuche-Berenguer, B. et al. (2015). Elucidation of the roles of the Src kinases in pancreatic acinar cell signaling. J Cell Biochem. 116:22-36.
    11. Sanchez-Lugo, Y. E. et al. (2014). CXCL10/XCL1 fusokine elicits in vitro and in vivo chemotaxis. Biotechnol Lett. doi:10.1007/s10529-014-1746-4. 
    12. Smith, M. et al. (2010). PRDM1/Blimp-1 Controls Effector Cytokine Production in Human NK Cells. J. Immunol. 185:6058-6067.
    13. Reiter, C.E.N. et al. (2010). Green tea polyphenol epigallocatechin gallate reduceds endothelin 1 expression and secretion in vascular endothelial cells: roles for AMP-activated protein kinase, Akt, and FOXO1. Endocrinology 151:103-114.
    14. Kang, S-H. et al. (2008). Roles of ERK and NFkB in interleukin-8 expression in response to heat shock protein 22 in vascular smooth muscle cells. Korean J. Physiol. Pharmacol. 12:171-176.
    15. Rho, M. et al. (2007). Sensitization of vascular smooth muscle cell to TNF-alpha-mediated death in the presence of palmitate. Toxicology and Applied Pharmacology 220(3):311-319.
    16. Bian, D. et al. (2006). The G12/13-RhoA signaling pathway contributes to efficient lysophosphatidic acid-stimulated cell migration. Oncogene 25:2234-2244.
VPK-110 　
    1. Teves, M.E. et al. (2020). Human DENND1A.V2 Drives Cyp17a1 Expression and Androgen Production in Mouse Ovaries and Adrenals. Int J Mol Sci. 21(7). pii: E2545. doi: 10.3390/ijms21072545.
    2. Guo, Y. et al. (2019). Menstrual blood-derived stem cells as delivery vehicles for oncolytic adenovirus virotherapy for colorectal cancer. Stem Cells Dev. doi: 10.1089/scd.2018.0222.
    3. Wu, Y. et al (2017). Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice. Antiviral Research. 147: 29-36.
    4. Kirshmer, N. et al. (2016). TRPC4a and TRPC4ß similarly affect neonatal cardiomyocyte survival during chronic GPCR stimulation. PLoS One 11:e0168446.
    5. García-Pascual, C. M. et al. (2015). Evaluation of the potential therapeutic effects of a double-stranded RNA mimic complexed with polycations in an experimental mouse model of endometriosis. Fertil Steril. doi:10.1016/j.fertnstert.2015.07.1147.
    6. Gibson, H. et al. (2015). Immunotherapeutic intervention with oncolytic adenovirus in mouse mammary tumors. OncoImmunology. 4:e984523.
    7. Lakshmanan, J. et al. (2015). Glycogen synthase kinase 3 regulates IL-1β mediated iNOS expression in hepatocytes by down-regulating c-Jun. J Cell Biochem. 116:133-141.
    8. Haidari, M. et al. (2014). Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-β-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol. 13:112.
    9. Oh, D. et al. (2013). Overexpression of SPARC in Human Trabecular Meshwork Increases Intraocular Pressure and Alters Extracellular Matrix. Invest. Ophthalmol. Visc. Sci. 54: 3309-3319.
    10. Muruganandan, S. et al. (2011). Chemerin, a Novel Peroxisome Proliferator-activated Receptor γ (PPARγ) Target Gene that Promotes Mesenchymal Stem Cell Adipogenesis. J. Biol. Chem. 286:23982-23995.
    11. Hoashi, T. et al. (2010). The Secreted Form of a Melanocyte Membrane-Bound Glycoprotein (Pmel17/gp100) is Released by Ectodomain Shedding. FASEB J. 10.1096/fj.09-140921 (#VPK-110).
    12. Tudhope, S. et al. (2007). The Role of IkB Kinase 2, but not Activation of NF-kB, in the Release of CXCR3 Ligands from IFN-Gamma-Stimulated Human Bronchial Epithelial Cells. J. Immunol. 179:6237-6245.
VPK-109 　
    1. McQuerry, J.A. et al. (2021). Tepoxalin increases chemotherapy efficacy in drug-resistant breast cancer cells overexpressing the multidrug transporter gene ABCB1. Transl Oncol. 14(10):101181. doi: 10.1016/j.tranon.2021.101181.
    2. Tournier, B.B. et al. (2021). Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes. ACS Omega. doi: 10.1021/acsomega.1c01678.
    3. Oliveira, E.R.A. et al (2021). Intracellular Sequestration of the NKG2D Ligand MIC B by Species F Adenovirus. Viruses. 13(7):1289. doi: 10.3390/v13071289.
    4. Borrás, T. et al. (2020). Generation of a Matrix Gla (Mgp) floxed mouse, followed by conditional knockout, uncovers a new Mgp function in the eye. Sci Rep. 10(1):18583. doi: 10.1038/s41598-020-75031-7.
    5. Wei, W.Z. et al. (2020). Diversity Outbred Mice Reveal the Quantitative Trait Locus and Regulatory Cells of HER2 Immunity. J Immunol. doi: 10.4049/jimmunol.2000466.
    6. Robles-Rodríguez, O.A. et al. (2020). Antitumor effect of adenoviruses expressing mutant nononcogenic E7 versions from HPV-16 fused to calreticulin. JBUON. 25(1): 543-548.
    7. Rollier, C.S. et al. (2020). Modification of Adenovirus vaccine vector-induced immune responses by expression of a signalling molecule. Sci Rep. 10(1):5716. doi: 10.1038/s41598-020-61730-8.
    8. Lokhandwala, S. et al. (2019). Adenovirus-vectored African Swine Fever Virus antigen cocktails are immunogenic but not protective against intranasal challenge with Georgia 2007/1 isolate. Veterinary Microbiology. 235:10-20. doi:10.1016/j.vetmic.2019.06.006.
    9. Sang, Y. et al. (2019). Ileal transcriptome analysis in obese rats induced by high-fat diets and an adenoviral infection. Int J Obes (Lond). doi: 10.1038/s41366-019-0323-2.
    10. Hao, J. et al. (2018). Prostate Cancer-Specific of DD3-driven Oncolytic Virus-harboring mK5 Gene. Open Med (Wars). 14: 1-9. doi: 10.1515/med-2019-0001.
    11. Mao, L.J. et al. (2018). Oncolytic Adenovirus Harboring Interleukin-24 Improves Chemotherapy for Advanced Prostate Cancer. J Cancer. 9(23):4391-4397. doi: 10.7150/jca.26437.
    12. Konishi, K. et al. (2018). In vitro approach to elucidate the relevance of carboxylesterase 2 and N-acetyltransferase 2 to flupirtine-induced liver injury. Biochem Pharmacol. 155:242-251. doi: 10.1016/j.bcp.2018.07.019.
    13. Que, W. et al. (2018). NK4 inhibits the proliferation and induces apoptosis of human rheumatoid arthritis synovial cells. Cell Biochem Funct. 36(5):273-279. doi: 10.1002/cbf.3339.
    14. Alharbi, N.K. et al. (2017). ChAdOx1 and MVA based vaccine candidates against MERS-CoV elicit neutralising antibodies and cellular immune responses in mice. Vaccine. 35(30):3780-3788.
    15. Xu, Y. et al. (2017). RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion. Cell Death Dis. 8(6):e2835.
    16. Yuan, S. et al. (2017). An oncolytic adenovirus expressing SNORD44 and GAS5 exhibits anti-tumor effect in colorectal cancer cells. Hum. Gene Ther. doi:10.1089/hum.2017.041.
    17. Lokhandwala, S. et al. (2017). Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine. PLoS One. 12(5):e0177007.
    18. Sugase T, et al. (2017). Suppressor of cytokine signaling-1 gene therapy induces potent antitumor effect in patient-derived esophageal squamous cell carcinoma xenograft mice. Int J Cancer. doi: 10.1002/ijc.30666.
    19. Keshava, S. et al. (2016). Intrapleural adenoviral-mediated endothelial cell protein C receptor gene transfer suppresses the progression of malignant pleural mesothelioma in a mouse model. Sci. Rep. 6:36829.
    20. Xiong, X. et al. (2016). SIRT6 protects against palmitate-induced pancreatic ß-cell dysfunction and apoptosis. J. Endocrinol. 231:159-165.
    21. Lokhandwala, S. et al. (2016). Induction of robust immune responses in swine using a cocktail of adenovirus-vectored african swine fever virus antigens. Clin Vaccine Immunol.doi:10.1128/CVI.00395-16.
    22. Ansari, S. A. et al. (2016). The role of putative phosphatidylserine-interactive residues of tissue factor on its coagulant activity at the cell surface. PLoS One. doi:10.1371/journal.pone.0158377.
    23. Martin, L. J. & Wong, W. (2016). Enforced DNA repair enzymes rescue neurons from apoptosis induced by target deprivation and axotomy in mouse models of neurodegeneration. Mech Ageing Dev. doi:10.1016/j.mad.2016.06.011.
    24. Gibot, L. et al. (2015). Cell-based approach for 3D reconstruction of lymphatic capillaries in vitro reveals distinct functions of HGF and VEGF-C in lymphangiogenesis. Biomaterials. doi:10.1016/j.biomaterials.2015.11.027.
    25. Herath, S. et al. (2015). Analysis of T cell responses to chimpanzee adenovirus vectors encoding HIV gag–pol–nef antigen. Vaccine. doi:10.1016/j.vaccine.2015.10.111.
    26. Yang, Y. et al. (2015). RGD-modifided oncolytic adenovirus exhibited potent cytotoxic effect on CAR-negative bladder cancer-initiating cells. Cell Death Dis.6:e1760.
    27. Nakao, S. et al. (2015).  Stimulus-dependent regulation of nuclear Ca 2+ signaling in cardiomyocytes: a role of neuronal calcium sensor-1. PLoS One. 10:e0125050.
    28. Patsouris, D. et al. (2014). Insulin resistance is associated with MCP1-mediated macrophage accumulation in skeletal muscle in mice and humans. PLoS One. 9:e110653.
    29. Xiong, Y. et al. (2014). Long term exposure to L-arginine accelerates endothelial cell senescence through arginase-II and S6K1 signaling. Aging (Albany NY). 6:369.
    30. Barrett. A. et al. (2014).  A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci. 127:2647-2658.


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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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