제품 상세 렌티바이러스 정량 | Lentivirus Titer & Quantitation Kits 전통적인 lentivirus 정량 방법인 Traditional p24 ELISA Kit, virus-associated p24만 정량하는 QuickTiter™ Lentivirus Titer Kit, 염색하여 확인하는 Lentivirus Rapid Quantitation등의 방법을 소개합니다. 프린트 제품 문의 QuickTiter™ Lentivirus Titer Kit (#VPK-107) Lentivirus를 pull down 시킨 후 p24 항체를 이용하여 정량합니다. free p24를 제외한 virus-associated p24만 정량하기 때문에 전통적인 p24 ELISA kit에 비해 lentivirus titer의 overestimation을 최소화 합니다.일반적인 microplate reader를 이용하여 정량할 수 있습니다.구성 : 1. ViraBind™ Lentivirus Reagent A2. ViraBind™ Lentivirus Reagent B 3. Sample Diluent4. Anti-p24 Antibody Coated Plate 5. FITC-Conjugated Anti-p24 Monoclonal Antibody6. HRP-Conjugated Anti-FITC Monoclonal Antibody7. Assay Diluent 8. 10X Wash Buffer 9. Substrate Solution 10. Stop Solution 11. Recombinant p24 Standard Virus Associated p24 Titer of GFP Lentiviral Supernatant. A GFP lentiviral construct was cotransfected with a packaging mix into 293LTV cells (Cat.# LTV-100). The conditioned medium was harvested 48 hrs after transfection. GFP expression was shown in HEK293 cells infected with the GFP lentiviral samples for 3 days (A: GFP lentiviral supernatant; B: ViraBind™ pellet). Free p24 and Virus Associated p24 were separated by ViraBind™ Lentivirus Reagents. The p24 level wasdetermined as described in the assay instructions.Traditional p24 ELISA Kit (#VPK-108) Recombinant HIV-1 based lentivirus의 모든 p24 core protein을 측정합니다. Sensitivity : 1 ng/mL HIV p24, or about 10,000 to 100,000 TU/mL VSVG-pseudotyped lentivirus samples구성 : 1. Anti-p24 Antibody Coated Plate2. FITC-Conjugated Anti-p24 Monoclonal Antibody 3. HRP-Conjugated Anti-FITC Monoclonal Antibody 4. Assay Diluent5. Triton X-100 Solution 6. 10X Wash Buffer 7. Substrate Solution8. Stop Solution 9. Recombinant p24 Standardp24 Titer of GFP Lentiviral Supernatant. A GFP lentiviral construct was cotransfected with a packaging mix into 293AD cells (Cat.# AD-100). The conditioned medium was harvested 48 hrs after transfection. GFP expression was shown in HEK293 cells infected with the GFP lentiviral supernatant for 3 days. The p24 level in the diluted lentiviral supernatant (1:10 dilution) was determined as described in the assay instructions.Lentivirus Rapid Quantitation Kit (VPK-112)형광 dye를 이용하여 염색 후 형광 plate를 바로 읽는 방식입니다. 45-60분 사이에 신속히 결과를 얻을 수 있습니다.Limit of detection: 1 X 10^10 VP/mL (mid-high titer sample에 이용)구성 : 1. QuickTiter™ Solution A 2. QuickTiter™ Lentivirus Capture Solution 3. QuickTiter™ Solution B4. QuickTiter™ Solution C 5. CyQuant® GR Dye 6. QuickTiter™ Lentivirus RNA Standard 사용논문 VPK-107 Leach, A. et al. (2021). A tetrameric ACE2 protein broadly neutralizes SARS-CoV-2 spike variants of concern with elevated potency. Antiviral Res. 194:105147. doi: 10.1016/j.antiviral.2021.105147. Kumar, S. et al. (2021). 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Product Cat.No. Size Maker Qty Data Sheet MSDS QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) VPK-108-H 96 assays CELL BIOLABS - + 보기 보기 QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) VPK-108-H-5 5x96 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Lentivirus Quantitation Kit VPK-112 20 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) VPK-107 96 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) VPK-107-5 5x96 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA), Trial Size VPK-107-T 32 assays CELL BIOLABS - + 보기 보기 Lentivirus Titer p24 ELISA Kit L00938 96Test GENSCRIPT - + 보기 보기 1 Maker CELL BIOLABS Cat.No. VPK-108-H Product QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) Size 96 assays Qty - + Data Sheet 보기 MSDS 보기 2 Maker CELL BIOLABS Cat.No. VPK-108-H-5 Product QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) Size 5x96 assays Qty - + Data Sheet 보기 MSDS 보기 3 Maker CELL BIOLABS Cat.No. VPK-112 Product QuickTiter™ Lentivirus Quantitation Kit Size 20 assays Qty - + Data Sheet 보기 MSDS 보기 4 Maker CELL BIOLABS Cat.No. VPK-107 Product QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) Size 96 assays Qty - + Data Sheet 보기 MSDS 보기 5 Maker CELL BIOLABS Cat.No. VPK-107-5 Product QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) Size 5x96 assays Qty - + Data Sheet 보기 MSDS 보기 6 Maker CELL BIOLABS Cat.No. VPK-107-T Product QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA), Trial Size Size 32 assays Qty - + Data Sheet 보기 MSDS 보기 7 Maker GENSCRIPT Cat.No. L00938 Product Lentivirus Titer p24 ELISA Kit Size 96Test Qty - + Data Sheet 보기 first 1 last 추천제품 LentiBOOST | Lentiviral Transduction Enhancer AdenoBOOST - Adenoviral Transduction Enhancer Ultra-titer Lentivirus 바이러스 제작서비스 | AAV, AV, LV | Virus Production Service 세포주 제작 서비스 | Stable Cell Line Generation Service 관련제품 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS QuickTiter™ AAV Quantitation Kit VPK-145 20 assays CELL BIOLABS - + 보기 보기 QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) VPK-108-H 96 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Lentivirus Quantitation Kit VPK-112 20 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Adenovirus Quantitation Kit VPK-106 20 assays CELL BIOLABS - + 보기 보기 QuickTiter™ Retrovirus Quantitation Kit VPK-120 20 assays CELL BIOLABS - + 보기 보기 1 Maker CELL BIOLABS Cat.No. VPK-145 Product QuickTiter™ AAV Quantitation Kit Size 20 assays Qty - + Data Sheet 보기 MSDS 보기 2 Maker CELL BIOLABS Cat.No. VPK-108-H Product QuickTiter™ HIV Lentivirus Quantitation Kit (p24 ELISA) Size 96 assays Qty - + Data Sheet 보기 MSDS 보기 3 Maker CELL BIOLABS Cat.No. VPK-112 Product QuickTiter™ Lentivirus Quantitation Kit Size 20 assays Qty - + Data Sheet 보기 MSDS 보기 4 Maker CELL BIOLABS Cat.No. VPK-106 Product QuickTiter™ Adenovirus Quantitation Kit Size 20 assays Qty - + Data Sheet 보기 MSDS 보기 5 Maker CELL BIOLABS Cat.No. VPK-120 Product QuickTiter™ Retrovirus Quantitation Kit Size 20 assays Qty - + Data Sheet 보기 MSDS 보기 자료 웨비나/Video
LentiBOOST | Lentiviral Transduction Enhancer AdenoBOOST - Adenoviral Transduction Enhancer Ultra-titer Lentivirus 바이러스 제작서비스 | AAV, AV, LV | Virus Production Service 세포주 제작 서비스 | Stable Cell Line Generation Service