고마바이오텍(주)

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Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

QuickTiter™ Lentivirus Titer Kit (#VPK-107)

  

  • Lentivirus를 pull down 시킨 후 p24 항체를 이용하여 정량합니다. 
  • free p24를 제외한 virus-associated p24만 정량하기 때문에 전통적인 p24 ELISA kit에 비해 lentivirus titer의 overestimation을 최소화 합니다.
  • 일반적인 microplate reader를 이용하여 정량할 수 있습니다.
  • 구성 :
    1. ViraBind™ Lentivirus Reagent A
    2. ViraBind™ Lentivirus Reagent B
    3. Sample Diluent
    4. Anti-p24 Antibody Coated Plate
    5. FITC-Conjugated Anti-p24 Monoclonal Antibody
    6. HRP-Conjugated Anti-FITC Monoclonal Antibody
    7. Assay Diluent
    8. 10X Wash Buffer
    9. Substrate Solution
    10. Stop Solution
    11. Recombinant p24 Standard 






Virus Associated p24 Titer of GFP Lentiviral Supernatant. A GFP lentiviral construct was cotransfected with a packaging mix into 293LTV cells (Cat.# LTV-100). The conditioned medium was harvested 48 hrs after transfection. GFP expression was shown in HEK293 cells infected with the GFP lentiviral samples for 3 days (A: GFP lentiviral supernatant; B: ViraBind™ pellet). Free p24 and Virus Associated p24 were separated by ViraBind™ Lentivirus Reagents. The p24 level was
determined as described in the assay instructions.


Traditional p24 ELISA Kit (#VPK-108)

   

  • Recombinant HIV-1 based lentivirus의 모든 p24 core protein을 측정합니다. 
  • Sensitivity : 1 ng/mL HIV p24, or about 10,000 to 100,000 TU/mL VSVG-pseudotyped lentivirus samples
  • 구성 : 
    1. Anti-p24 Antibody Coated Plate
    2. FITC-Conjugated Anti-p24 Monoclonal Antibody
    3. HRP-Conjugated Anti-FITC Monoclonal Antibody
    4. Assay Diluent
    5. Triton X-100 Solution
    6. 10X Wash Buffer
    7. Substrate Solution
    8. Stop Solution
    9. Recombinant p24 Standard


p24 Titer of GFP Lentiviral Supernatant. A GFP lentiviral construct was cotransfected with a packaging mix into 293AD cells (Cat.# AD-100). The conditioned medium was harvested 48 hrs after transfection. GFP expression was shown in HEK293 cells infected with the GFP lentiviral supernatant for 3 days. The p24 level in the diluted lentiviral supernatant (1:10 dilution) was determined as described in the assay instructions.


Lentivirus Rapid Quantitation Kit (VPK-112)

  • 형광 dye를 이용하여 염색 후 형광 plate를 바로 읽는 방식입니다. 
  • 45-60분 사이에 신속히 결과를 얻을 수 있습니다.
  • Limit of detection: 1 X 10^10 VP/mL (mid-high titer sample에 이용)
  • 구성 :
    1. QuickTiter™ Solution A
    2. QuickTiter™ Lentivirus Capture Solution
    3. QuickTiter™ Solution B
    4. QuickTiter™ Solution C
    5. CyQuant® GR Dye
    6. QuickTiter™ Lentivirus RNA Standard 





사용논문

VPK-107 　
    1. Leach, A. et al. (2021). A tetrameric ACE2 protein broadly neutralizes SARS-CoV-2 spike variants of concern with elevated potency. Antiviral Res. 194:105147. doi: 10.1016/j.antiviral.2021.105147. 
    2. Kumar, S. et al. (2021). In Vivo Lentiviral Gene Delivery of HLA-DR and Vaccination of Humanized Mice for Improving the Human T and B Cell Immune Reconstitution. Biomedicines. 9(8):961. doi: 10.3390/biomedicines9080961.
    3. Riethmüller, D. et al. (2021). Scalable upstream process development for the suspension-based production of lentiviral vectors for CAR T cell therapies with multiparallel & benchtop bioreactor systems & DoE methodology. Cell Gene Ther Insights. 7(6):689–700. doi: 10.18609/cgti.2021.099.
    4. Tulotta, C. et al. (2021). IL-1B drives opposing responses in primary tumours and bone metastases; harnessing combination therapies to improve outcome in breast cancer. NPJ Breast Cancer. 7(1):95. doi: 10.1038/s41523-021-00305-w.
    5. Valverde, A. et al. (2021). Dipeptidyl peptidase 4 contributes to Alzheimer’s disease-like defects in a mouse model and is increased in sporadic Alzheimer’s disease brains. J Biol Chem. doi: 10.1016/j.jbc.2021.100963.
    6. Torres, A.G. et al. (2021). Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins. Nucleic Acids Res. doi: 10.1093/nar/gkab461.
    7. Lyu, P. et al. (2021). Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like Particles Show High On-Target Base Editing and Undetectable RNA Off-Target Activities. CRISPR J. 4(1):69-81. doi: 10.1089/crispr.2020.0095.
    8. Li, H. et al. (2021). A Rat Model of EcoHIV Brain Infection. J. Vis. Exp. 167:e62137. doi: 10.3791/62137.
    9. Van Cleemput, J. et al. (2020). CRISPR/Cas9-constructed pseudorabies virus mutants reveal the importance of UL13 in alphaherpesvirus escape from genome silencing. J Virol. doi: 10.1128/JVI.02286-20.
    10. Cadima-Couto, I. et al. (2020). Anti-HIV-1 Activity of pepRF1, a Proteolysis-Resistant CXCR4 Antagonist Derived from Dengue Virus Capsid Protein. ACS Infect Dis. doi: 10.1021/acsinfecdis.9b00507.
    11. Wu, J. et al. (2020). Requisite Chromatin Remodeling for Myeloid and Erythroid Lineage Differentiation from Erythromyeloid Progenitors. Cell Rep. 33(7):108395. doi: 10.1016/j.celrep.2020.108395.
    12. Gardell, J.L. et al. (2020). Human macrophages engineered to secrete a bispecific T cell engager support antigen-dependent T cell responses to glioblastoma. J Immunother Cancer. 8(2):e001202. doi: 10.1136/jitc-2020-001202.
    13. Narayan, P. et al. (2020). PICALM Rescues Endocytic Defects Caused by the Alzheimer's Disease Risk Factor APOE4. Cell Rep. 33(1):108224. doi: 10.1016/j.celrep.2020.108224.
    14. Lyu, P. et al. (2020). Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay. PLoS One. 15(9):e0239468. doi: 10.1371/journal.pone.0239468.
    15. Choi, J.A. et al. (2020). Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike DNA and Protein. J Virol. doi: 10.1128/JVI.01176-20.
    16. Hoffmann, M.A.G. et al. (2020). Nanoparticles presenting clusters of CD4 expose a universal vulnerability of HIV-1 by mimicking target cells. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.2010320117.
    17. Fernandes-Junior, S.A. et al. (2020). Stimulation of Retrotrapezoid Nucleus Phox2b-expressing Neurons Rescues Breathing Dysfunction in an Experimental Parkinson's Disease Rat Model. Brain Pathol. doi: 10.1111/bpa.12868.
    18. Folegatti, P.M. et al. (2020). Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial. Lancet Infect Dis. pii: S1473-3099(20)30160-2. doi: 10.1016/S1473-3099(20)30160-2.
    19. Purroy, R. et al. (2020). Frataxin-deficient cardiomyocytes present an altered thiol-redox state which targets actin and pyruvate dehydrogenase. Redox Biology. 32:101520. doi: 10.1016/j.redox.2020.101520.
    20. Javidi-Parsijani, P. et al. (2020). CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. doi: 10.1038/s41434-020-0126-z.
    21. Yang, H. et al. (2020). Understanding the structural basis of HIV-1 restriction by the full length double-domain APOBEC3G. Nat Commun. 11(1):632. doi: 10.1038/s41467-020-14377-y.
    22. Goo, J. et al. (2020). Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. doi: 10.1016/j.virusres.2020.197863.
    23. Czarnek, M. et al. (2019). Proteolytic Processing of Neuregulin 2. Mol Neurobiol. doi: 10.1007/s12035-019-01846-9.
    24. Pan, X. et al. (2019). Lysine-specific demethylase-1 regulates fibroblast activation in pulmonary fibrosis via TGF-β1/Smad3 pathway. Pharmacol Res. 152:104592. doi: 10.1016/j.phrs.2019.104592.
    25. Bussiere, R. et al. (2019). Upregulation of the Sarco-Endoplasmic Reticulum Calcium ATPase 1 Truncated Isoform Plays a Pathogenic Role in Alzheimer's Disease. Cells. 8(12). pii: E1539. doi: 10.3390/cells8121539.
    26. Jha, A. et al. (2019). Alterations in Plasma Membrane Ion Channel Structures Stimulate NLRP3 Inflammasomes Activation in APOL1 Risk Milieu. FEBS J. doi: 10.1111/febs.15133.
    27. Zhang, S. et al. (2019). The resistance of esophageal cancer cells to paclitaxel can be reduced by the knockdown of long noncoding RNA DDX11-AS1 through TAF1/TOP2A inhibition. Am J Cancer Res. 9(10):2233-2248.
    28. Park, T.Y. et al. (2019). Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases. Sci Rep. 9(1):15559. doi: 10.1038/s41598-019-52085-w.
    29. Gonzalez, S.M. et al. (2019). Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS One. 14(9):e0222878. doi: 10.1371/journal.pone.0222878.
    30. Ruscic, J. et al. (2019). Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography. Mol Ther Methods Clin Dev. doi: 10.1016/j.omtm.2019.08.007.
VPK-108 　
    1. Liu, D. et al. (2021). Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel. Nat Commun. 12(1):2551. doi: 10.1038/s41467-021-22737-5.
    2. Kwon, O.C. et al. (2021). SGK1 inhibition in glia ameliorates pathologies and symptoms in Parkinson disease animal models. EMBO Mol Med. doi: 10.15252/emmm.202013076.
    3. Wulansari, N. et al. (2021). Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson's disease-linked DNAJC6 mutations. Sci Adv. 7(8):eabb1540. doi: 10.1126/sciadv.abb1540.
    4. Rodríguez-Pascau, L. et al. (2020). PPAR gamma agonist leriglitazone improves frataxin-loss impairments in cellular and animal models of Friedreich Ataxia. Neurobiol Dis. doi: 10.1016/j.nbd.2020.105162.
    5. Saulle, I. et al. (2020). A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells. Could It Play a Role in the Modulation of SARS-CoV-2 Infection?. Cells. 9(9):E1951. doi: 10.3390/cells9091951.
    6. Miwa, S. et al. (2020). Efficient engraftment of genetically modified cells is necessary to ameliorate central nervous system involvement of murine model of mucopolysaccharidosis type II by hematopoietic stem cell targeted gene therapy. Mol Genet Metab. doi: 10.1016/j.ymgme.2020.06.007.
    7. Pereira, M.S. et al. (2019). Loss of SPINT2 expression frequently occurs in glioma, leading to increased growth and invasion via MMP2. Cell Oncol (Dordr). doi: 10.1007/s13402-019-00475-7.
    8. Song, J.J. et al. (2018). Cografting astrocytes improves cell therapeutic outcomes in a Parkinson's disease model. J Clin Invest. 128(1):463-482. doi: 10.1172/JCI93924.
    9. Luo, J. et al. (2018). CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro. Int J Cancer. 143(12):3169-3180. doi: 10.1002/ijc.31734.
    10. Schlatzer, D. et al. (2017). A targeted mass spectrometry assay for detection of HIV gag protein following induction of latent viral reservoirs. Anal. Chem. 89(10):5325-5332.
    11. Bianco, C. et al. (2017). Restriction of HCMV replication by ISG15, a host effector regulated by cGAS-STING dsDNA sensing. J Virol. doi: 10.1128/JVI.02483-16.
    12. Bonar, M.M. et al. (2017). High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometry. Virology. doi: 10.1016/j.virol.2017.02.016.
    13. Chen, F. et al. (2017). Episomal lentiviral vectors confer erythropoietin expression in dividing cells. Plasmid. doi: 10.1016/j.plasmid.2017.02.001.
    14. Kieffer, C. et al. (2017). Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography. Elife. doi: 10.7554/eLife.23282.
    15. Li, J. et al. (2016). Artemisinins target GABA A receptor signaling and impair a cell identity. Cell doi:10.1016/j.cell.2016.11.010.
    16. Haqqani, A. A. et al. (2015). Central memory CD4+ T cells are preferential targets of double infection by HIV-1. Virol J.  12:184.
    17. Lin, B. et al. (2015). Use of a novel integrase-deficient lentivirus for targeted anti-cancer therapy with survivin promoter-driven diphtheria toxin A. Medicine (Baltimore). 94:e1301.
    18. Oh, S. M. et al. (2015). Combined Nurr1 and Foxa2 roles in the therapy of Parkinson's disease. EMBO Mol Med. doi: 10.15252/emmm.201404610. 
    19. Tilton, C. A. et al. (2014). A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsets.J Virol Methods. 195:164-169.
    20. Lucera, M.B. et al. (2014).  The Histone Deacetylase Inhibitor Vorinostat (SAHA) Increases the Susceptibility of Uninfected CD4+ T Cells to HIV by Increasing the Kinetics and Efficiency of Postentry Viral Events. J Virol. 88:10803-10812.
    21. Tabler, C.O. et al. (2014).  CD4+ Memory Stem Cells Are Infected by HIV-1 in a Manner Regulated in Part by SAMHD1 Expression. J Virol. 88:4976-4986.
    22. Mincheva-Tasheva, S. (2014). Apoptotic cell death and altered calcium homeostasis caused by frataxin depletion in dorsal root ganglia neurons can be prevented by BH4 domain of Bcl-xL protein. Hum. Mol. Genet. 23:1829-1841.
    23. Yi, S.H. et al. (2014). Foxa2 Acts as a Co-Activator Potentiating Expression of the Nurr1-Induced DA Phenotype via Epigenetic Regulation. Development. 141:761-772.
    24. Tasheva, S. et al. (2013). Apoptotic cell death and altered calcium homeostasis caused by frataxin depletion in dorsal root ganglia neurons can be prevented by BH4 domain of Bcl-xL protein. Human. Mol. Genet. doi: 10.1093/hmg/ddt576.
    25. Kong, L. et al. (2013). Interferon Alfa Partially Inhibits HIV Replication in Hepatocytes In Vitro. The Journal of Infectious Disease. 99:632-639.
    26. Smith, B. et al. (2013). Targeting the PyMT Oncogene to Diverse Mammary Cell Populations Enhances Tumor Heterogeneity and Generates Rare Breast Cancer Subtypes. Genes & Cancer. 10.1177/1947601913475359.
    27. Iftikhar, M. et al. (2011). Lysyl Oxidase-like-2 (LOXL2) is a Major Isoform in Chondrocytes and is Critically Required for Differentiation. J. Biol. Chem. 286:909-918.
    28. Agrawai-Gamse, C. et al. (2010). Yeast-elicited cross-reactive antibodies to HIV Env glycans efficiently neutralize virions expressing exclusively high-mannose N-Linked glycans. J. Virol. 85(1):470-480.
    29. Piedrahita, D. et al. (2010). Silencing of CDK5 Reduces Neurofibrillary Tangles in Transgenic Alzheimer's Mice. J. Neurosci. 30:13966-13976.
    30. Young, A.P. and Wagers, A.J. (2010). Pax3 Induces Differentiation of Juvenile Skeletal Muscle Stem Cells Without Transcriptional Upregulation of Canonical Myogenic Regulatory Factors. J. Cell Sci. 123: 2632-2639.
VPK-112 　
    1. Bonar, M.M. et al. (2020). Nanoscale flow cytometry reveals interpatient variability in HIV protease activity that correlates with viral infectivity and identifies drug-resistant viruses. Sci Rep. 10(1):18101. doi: 10.1038/s41598-020-75118-1.
    2. Li, J. et al. (2020). Nna1 gene deficiency triggers Purkinje neuron death by tubulin hyperglutamylation and ER dysfunction. JCI Insight. 5(19):136078. doi: 10.1172/jci.insight.136078.
    3. Huang, L. et al. (2019). Time-restricted release of multiple neurotrophic factors promotes axonal regeneration and functional recovery after peripheral nerve injury. FASEB J. fj201802065RR. doi: 10.1096/fj.201802065RR.
    4. Huang, L. et al. (2018). Beta-catenin promotes macrophage-mediated acute inflammatory response after myocardial infarction. Immunol Cell Biol. 96(1):100-113. doi: 10.1111/imcb.1019.
    5. Ghosh, N. & Banerjee, E.R. (2017). A Review on Various Tissue Engineering Techniques to Induce Differentiation of Pluripotent Stem Cells. Medical Glory. 1(2):130-149.
    6. Pardee, K. et al. (2016). Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell. 165:1-12.
    7. Luo, H. et al. (2016). EphrinB1 and EphrinB2 regulate T cell chemotaxis and migration in experimental autoimmune encephalomyelitis and multiple sclerosis. Neurobiol Dis. 91:292-306.
    8. Georgiadis, M. M. et al. (2016). Small molecule activation of apurinic/apyrimidinic endonuclease 1 reduces DNA damage induced by cisplatin in cultured sensory neurons. DNA Repair.  doi:10.1016/j.dnarep.2016.03.009.
    9. Lambert, M. P. et al. (2015). Intramedullary megakaryocytes internalize released platelet factor 4 (PF4) and store it in alpha granules. J Thromb Haemost. doi:10.1111/jth.13069. 
    10. Kim, H. Suk. et al. (2015). APE1, the DNA base excision repair protein, regulates the removal of platinum adducts in sensory neuronal cultures by NER.Mutat Res-Fund Mol M. doi:10.1016/j.mrfmmm.2015.06.010.
    11. Yadavilli, S. et al. (2015). The emerging role of NG2 in pediatric diffuse intrinsic pontine glioma. Oncotarget.  6:12141-12155.
    12. Ahmad, M. A. et al. (2015). Label-free capacitance-based identification of viruses. Sci Rep.5:9809.
    13. Tang, X. et al. (2015). The advantages of PD1 activating chimeric receptor (PD1-ACR) engineered lymphocytes for PDL1+ cancer therapy. Am J Transl Res. 7:460-473.
    14. Giraldo, D. M. et al. (2015).  Impact of in vitro costimulation with TLR2, TLR4 and TLR9 agonists and HIV-1 on antigen-presenting cell activation. Intervirology. 58:122-129.
    15. Shin, H. S. et al. (2015). Crosstalk among IL-23 and DNAX activating protein of 12 kDa-dependent pathways promotes osteoclastogenesis.J Immunol. 194:316-324.
    16. Al Ahmad, M. et al. (2014). Virus detection and quantification using electrical parameters. Sci Rep. 4:6831.
    17. Fan, X. et al. (2014). Endometrial VEGF induces placental sFLT1 and leads to pregnancy complications. J Clin Invest. 124:4941-4952.
    18. Zhao, S. L. et al. (2014). Mesenchymal stem cells with overexpression of midkine enhance cell survival and attenuate cardiac dysfunction in a rat model of myocardial infarction. Stem Cell Res Ther. 5:37.
    19. Rossello, R.A. et al. (2013). Mammalian Genes Induce Partially Reprogrammed Pluripotent Stem Cells in Non-Mammalian Vertebrate and Invertebrate Species. eLife Sci. 2:e00036.
    20. Fan, X. et al. (2012). Transient, Inducible, Placenta-Specific Gene Expression in Mice. Endocrinology. 153: 5637-5644 (#VPK-112). 
    21. Veeraraghavalu, K. et al. (2010). Presenilin 1 Mutants Impair the Self-Renewal and Differentiation of Adult Murine Subventricular Zone-Neuronal Progenitors via Cell-Autonomous Mechanisms Involving Notch Signaling. J. Neurosci. 30:6903-6915.
    22. Niwano, K. et al. (2008). Lentiviral vector-mediated SERCA2 gene transfer protects against heart failure and left ventricular remodeling after myocardial infarction in rats. Mol. Ther. 16:1026-1032.


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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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