[NETRI] Organs-on-chip (OoC) 연구를 위한 microfluidic device | 고마바이오텍

특징

"NETRI, Digitizing human biology"

• NETRI의 미세유체장치는 체외 분석이나 생체내 실험을 대체하여 화합물이나 화장품 원료/완제품의 효능과 독성을 이해하는데 이용됩니다.
  
• 서로 다른 세포를 각각 독립적으로 분화시키고 관찰할 수 있도록 분획(compartmentalization)되어 있고, 각 분획은 microchannel로 연결됩니다. 따라서 각 세포별 화합물에 따른 반응 결과를 모니터링 할 수 있습니다. 
  
• 단일 칩에서 동일한 장기의 다른 영역의 생리적 행동, 조직과 다른 조직의 상호 작용 또는 복잡한 신경망을 재현합니다. (예, neuron과 muscle cell의 조합으로 ALS 환경 재현)
• 칩에 사용된 PDMS silicone 재질은 높은 광학적 투과성과 생체적합성을 보장합니다.
• Porous polycarbonate membrane은 세포의 diffusion, exchange, communication을 조절합니다. 
• Functional synapses recording등  neural network activity를 측정하기 위한 MEA format으로도 제공되어 cell에서의 화합물의 효능을 기능적으로 측정할 수 있습니다.

"NETRI, innovative technologies"

• NETRI 장치는 높은 생체 적합성과 광학적 투명성을 위해 PDMS로 만들어졌으며, SBS 호환성을 갖는 형태로 제공되어 표준 실험실 장비에 바로 호환됩니다. 
• NETRI 장치는 두 가지 유형의 기술이 이용됩니다.
  1. Axon에 의해 모든 organ의 신경 분포를  가능하게 하는 마이크로채널
  2. 모든 organ의 혈관신생을 위한 다공성 막 

• 구획화는 다양한 세포 유형(뉴런, 각질세포, 내피 세포 등)을 독립적으로 성장시킬 수 있을 뿐만 아니라 모든 기관의 신경 분포 및 혈관 형성을 허용합니다. 
• NETRI 장치는 MEA(MicroElectrode Array) 기술과 호환되어 뉴런의 전기 활동을 기록할 수 있습니다. NETRI는 전기생리학 분야의 세계적인 선두주자인 Axion BioSystems와 협력하여 장치를 개발했습니다.

종류  

• Neurodegenerative disease 연구를 위한 pre-clinical organs-on-chip 제작에 이용
• 적용 : Drug screening, Axotomy, Neuroinflammation, Skin nociception, Embryology, Virology, Parkinson's disorders, ALS, Huntington's disease 

1) DuaLink

• 3 compartments : 2 for cell culture & 1 for fluidic isolation
• Co-culture of different cell types
• Compatible with lab equipments and imaging & biochimic analysis readouts (ELISA, Live Staining etc…)
• Discontinious connectivity by microchannels tunnels technology
• Wide array of application from innervated skin 2D modeling, neuromuscular junction modeling to toxicology or virology. 
• FULL format for 16 data points or LIGHT format for 8 data points

2) DuaLink Shift

• 3 compartments : 2 for cell culture & 1 for synaptic isolation or 1 for cell culture & 2 for quantification of axonal growth
• Co-culture of different cell types
• Enhanced growth kinetics
• Compatible with lab equipments and imaging & biochimic analysis readouts (ELISA, Live Staining etc…)
• Discontinious connectivity by microchannels tunnels technology
• Wide array of application from Nerve Injury & regrowth after trauma, Alzheimer’s diseases modeling, embryology to axonal regeneration visualization and quantification. 
• FULL format for 16 data points or LIGHT format for 8 data points

3) TriaLink

2. NeuroFluidics™ MEA Line 

• NeuroFluidics™ Line에 electrophysiology recording을 위한 MEA 기능을 탑재한 모델 

1) DuaLink MEA 

• 3 compartments integrated on a Axion BioSystems’ MEA layer : 2 for cell culture & 1 for fluidic isolation
• Co-culture of different cell types & neurons functional activity recording
• Compatible with lab equipments and electrophysiology, imaging & biochimic analysis readouts (Electrophysiology, ELISA, Live Staining etc…)
• Electrodes on each compartments and microchannels (Ch 1, 1µ2, Ch 2, 2µ3 and Ch3)
• UpLink utility software to output all MEA metrics in a highly versatile document
• Wide array of application from sensory neurons electrical activity, Parkinson’s disease modeling to functional innervated skin 2D modeling. 
• PRO format for 16 data points or EDGE format for 8 data points

2) DuaLink Shift MEA 

• 3 compartments integrated on a Axion BioSystems’ MEA layer : 2 for cell culture & 1 for synaptic isolation or 1 for cell culture & 2 for quantification of axonal growth
• Synapses electrophysology activity isolation per compartment 
• Compatible with lab equipments and electrophysiology, imaging & biochimic analysis readouts (Electrophysiology, ELISA, Live Staining etc…)
• Electrodes on each compartments and microchannels (Ch 1, 1µ2, Ch 2, 2µ3 and Ch3)
• UpLink utility software to output all MEA metrics in a highly versatile document
• Wide array of application from analysis of the functional influence of a non-neuronal cell population on synapse, mitochondrial transport to synaptic compartmentalization (pre-, post- and synaptic compartments)
• PRO format for 16 data points or EDGE format for 8 data points

3) TriaLink MEA  

• 3 compartments integrated on a Axion BioSystems’ MEA layer : 3 for cell culture
  
• Co-culture of different cell types & neurons functional activity recording
• Compatible with lab equipments and electrophysiology, imaging & biochimic analysis readouts (Electrophysiology, ELISA, Live Staining etc…)
• Electrodes on each compartments and microchannels (Ch 1, 1µ2, Ch 2, 2µ3 and Ch3)
• UpLink utility software to output all MEA metrics in a highly versatile document
• Wide array of application from the analysis of the functional influence of a non-neuronal cell population (astrocyte cells for example) on neurons to Stress effect on skin cells (ROS…). 
• PRO format for 16 data points or EDGE format for 8 data points

• NeoBento™ HT Format에 MEA 호환성을 추가한 device
• Electrophysiology readout 가능

1. Pain & Injury Models

Chemotherapy-Induced Peripheral Neuropathy

• DuaLink compartmentalized organs-on-chip
• hiPSCs sensory neurons
• Screening services with your compounds
  
• R&D co-development services

Nerve Injury

• DuaLink Shift compartmentalized organs-on-chip
• hiPSCs sensory neurons OR hiPSCs motor neurons
• Nocodazole & Staurosporine as a reference compound (results available here)
• Screening services with your compounds
• R&D co-development services