Organs-on-chip (OoC) 연구를 위한 microfluidic device | 고마바이오텍

특징

• NETRI의 미세유체장치는 체외 분석이나 생체내 실험을 대체하여 화합물의 효능과 독성을 이해하는데 이용됩니다.
• 칩에 사용된 PDMS silicone 재질은 높은 광학적 투과성과 생체적합성을 보장합니다.
• 서로 다른 세포를 각각 독립적으로 분화시키고 관찰할 수 있도록 분획(compartmentalization)되어 있습니다.
• 각 분획은 microchannel tunnel이나 groove를 통해 연결됩니다. (microchannel tunnel은 fluidic isolation용으로, microchannel groove는 axon의 continuous connectivity 용으로 적합합니다.)
• 단일 칩에서 동일한 장기의 다른 영역의 생리적 행동, 조직과 다른 조직의 상호 작용 또는 복잡한 신경망을 재현합니다. (예, neuron과 muscle cell의 조합으로 ALS 환경 재현)
• Porous polycarbonate membrane은 세포의 diffusion, exchange, communication을 조절합니다. 
  
• Functional synapses recording등  neural network activity를 측정하기 위한 MEA format으로도 제공됩니다.

• Neurodegenerative disease 연구를 위한 pre-clinical organs-on-chip 제작에 이용
• 적용 : Drug screening, Axotomy, Neuroinflammation, Skin nociception, Embryology, Virology, Parkinson's disorders, ALS, Huntington's disease 

1) DuaLink

• 3 compartments : 2 for cell culture & 1 for fluidic isolation

• 4 up to 16 microfluidic chips per NeoBento

• Co-culture of different cell types

• Compartmentalization

• Fluidic isolation

• Discontinious connectivity by microchannels tunnels technology

2) DuaLink Shift

• 3 compartments: 2 for cell culture & 1 for synaptic isolation

• 8 or 16 microfluidic chips per NeoBento™

• Synapse isolation in the middle channel

• Asymmetrical shape with microchannels of different length (shorter length between the first and second channel than between the second and third channel)

• Discontinious connectivity by microchannels tunnels technology

3) DuaLink Ultra

• 3 compartments : 2 for cell culture & 1 for fluidic isolation

• 8 or 16 microfluidic chips per NeoBento™

• Co-culture of different cell types

• Enhanced axonal projection and time to results

• Continuous connectivity by microchannels grooves technology

 

 

 

 

5) TriaLink

• 3 compartments for independant 3 different cell type cultures

• 8 or 16 microfluidic chips per NeoBento™

• Up to tri-culture of 3 different cell types

• Discontinuous connectivity by microchannels tunnels technology

6) TriaLink Ultra

• 3 compartments for independant 3 different cell type cultures

• 8 or 16 microfluidic chips per NeoBento™

• Up to tri-culture of different cell types

• Continuous connectivity by microchannels grooves technology

2. NeuroFluidics™ MEA Line 

• NeuroFluidics™ Line에 electrophysiology recording을 위한 MEA 기능을 탑재한 모델 

1) DuaLink MEA

• 3 compartments integrated on a custom Axion Biosystems MEA layer: 2 for cell culture & 1 for fluidic isolation

• 16 microfluidic chips per NeoBento™ MEA Pro with 672 electrodes

• 8 microfluidic chips per NeoBento™ MEA Edge with 336 electrodes

• Cell type electrophysology activity isolation per compartment

• Remote stimulation (compound or electrical) 

2) DuaLink Shift MEA

• 3 compartments integrated on a custom Axion Biosystems MEA layer: 2 for cell culture & 1 for synaptic isolation

• 16 microfluidic chips per NeoBento™ MEA Pro with 672 electrodes

• 8 microfluidic chips per NeoBento™ MEA Edge with 336 electrodes

• Asymmetrical shape with microchannels of different length

• Orientation of the network by preferentially allowing axons to develop from channel 3 to channel 1

• Synapses electrophysology activity isolation per compartment

3) TriaLink MEA 

• 3 compartments integrated on a custom Axion Biosystems MEA layer: 3 for cell culture

• 16 microfluidic chips per NeoBento™ MEA Pro with 672 electrodes

• 8 microfluidic chips per NeoBento™ MEA Edge with 336 electrodes

• Cell type electrophysology activity isolation per compartment

• Remote stimulation (compound or electrical)