성상교세포 | Astrocyte | 고마바이오텍

특징

• 성상교세포는 알츠하이머 및 파킨슨 병, 자폐증, 근 위축성 측삭 경화증 (ALS), 레트 증후군 및 조현병 같은 많은 신경학적 증상 연구에 관련이 있습니다.
• iPSC-Derived Astrocyte는 인간의 일차 성상교세포와 기능이 동등하여 in vitro 에서 신약개발 및 질병 모델링등 정확한 Astrocyte 연구를 도모할 수 있습니다. 
• 냉동보관되며, 일단 해동되면 48시간 내에 모든 Astrocyte-specific marker를 발현하고 48-60 시간 내에 주요 기능적 특성인 Glutamate 자극에 반응합니다.
  
• 단독으로, 혹은 중추신경계에 존재하는 다른 세포와의 co-culture가 가능합니다.
• GFAP, S100b, AQP4, ALDHL1L 및 EAAT1과 같은 프로토 타입 표현형 마커를 발현합니다.
• Vimentin, Kir4.1 (칼륨 채널) 및 CD44를 비롯한 다른 주요 biomarker도 발현합니다.

Phase contrast images of Axol Human iPSC-derived Astrocyte at day 1 in culture. Human iPSC-derived Astrocytes are seeded using Astrocyte Maintenance Medium into a 6-well plate.

iPSC-derived cerebral cortical interneurones (ax0667) inhibit and modulate activities of the excitatory neurons - less individual spikes, more bursts; iPSC-derived astrocytes (ax0665) provide a supportive, physiologically relevant environment where the cortical neurons exhibit faster maturation as demonstrated by larger spike amplitudes, more frequent spikes and more burst activities.

Axol iPSC-derived astrocytes express astrocyte-specific markers GFAP and S100B. Low expression of nestin, a marker of progenitor cell phenotype and MAP2, a marker of microtubule growth and neuritogenesis, indicates successful glial differentiation of the iPSCs. Cells seeded on Matrigel at 16,000 cells/well of a 96-well plate. Data provided by Susanne Zach, Boehringer Ingelheim.

Axol iPSC-derived astrocytes express astrocyte associated markers excitatory amino acid transporter 1 (EAAT1), glutamine synthetase (GS) and ALDH1L1. EAAT1 is active in clearing excitatory glutamate from the extracellular space, preventing neurotoxicity where GS converts glutamate to glutamine. The expression of these markers highlights that Axol’s Human iPSC-derived Astrocytes are physiologically representative of human primary astrocytes. Cells seeded on Matrigel at 16,000 cells/well of a 96-well plate. Data provided by Susanne Zach, Boehringer Ingelheim.