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Phagocytosis Assay Kit 이미지

Phagocytosis Assay Kit

CytoSelect™ 96-Well Phagocytosis Assay Kit 는 적혈구(RBC), Zymosan, E,coli를 세포 기질로 이용하여, 세포의 식균작용을 분석합니다.

특징

 

  • 적혈구(RBC), Zymosan, 또는 E.coli를 pathogen으로 이용하여, 세포의 식균작용을 유도하고 분석합니다. 
  • adherent phagocytes에는 Zymosan을, suspension cell의 식균작용에는 E.coli나 RBC를 이용합니다. 
  • 96-well 형식으로 제공되어 많은 샘플을 동시에 분석할 수 있습니다.
  • Colorimetric 방법을 이용하여 Standard microplate reader로 간편하게 측정할 수 있습니다.


Phagocytosis Process




분석 원리




종류

  • CytoSelect™ 96-Well Phagocytosis Assay (E.coli Substrate) (#CBA-222)
  • CytoSelect™ 96-Well Phagocytosis Assay (Red Blood Cell) (#CBA-220)
  • CytoSelect™ 96-Well Phagocytosis Assay (Zymosan) (#CBA-224)


사용 논문

  1. Park, E. et al. (2021). In Vivo Evaluation of Immune-Enhancing Activity of Red Gamju Fermented by Probiotic Levilactobacillus brevis KU15154 in Mice. Foods. 10(2):253. doi: 10.3390/foods10020253.
  2. Shin, J. et al. (2020). Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice. Nutrients. 12(8):E2260. doi: 10.3390/nu12082260.
  3. Vay, S.U. et al. (2020). The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function. J Neuroinflammation. 17(1):100. doi: 10.1186/s12974-020-01779-4.
  4. Zuo, P. et al. (2020). Protease-activated receptor 2 deficiency in hematopoietic lineage protects against myocardial infarction through attenuated inflammatory response and fibrosis. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2020.03.077.
  5. Trivedi, M.K. et al. (2020). Solid and liquid state characterization of tetrahydrocurcumin using XRPD, FT-IR, DSC, TGA, LC-MS, GC-MS, NMR and its biological activities. J Pharm Anal. doi: 10.1016/j.jpha.2020.02.005.
  6. Dashdulam, D. et al. (2020). Osteopontin heptamer peptide containing the RGD motif enhances the phagocytic function of microglia. Biochem Biophys Res Commun. pii: S0006-291X(20)30175-3. doi: 10.1016/j.bbrc.2020.01.100.
  7. Rabenstein, M. et al. (2020). Crosstalk between stressed brain cells: direct and indirect effects of ischemia and aglycemia on microglia. J Neuroinflammation. 17(1):33. doi: 10.1186/s12974-020-1697-8.
  8. Abumaree, M.H. et al. (2019). Decidua Basalis Mesenchymal Stem Cells Favor Inflammatory M1 Macrophage Differentiation In Vitro. Cells. 8(2). pii: E173. doi: 10.3390/cells8020173.
  9. Kim, S. et al. (2019). Immune-enhancing screening of fourteen plants on murine macrophage RAW 264.7 cells. Trop J Pharm Res. 18(1): 86. doi: 10.4314/tjpr.v18i1.13.
  10. Al-Kushi, A.G. et al. (2018). Antioxidant effect of royal jelly on immune status of hyperglycemic rats. Phcog Mag. 14:528-33. doi: 10.4103/pm.pm_87_18.
  11. Vay, S.U. et al. (2018). The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo. J Neuroinflammation. 15(1):226. doi: 10.1186/s12974-018-1261-y.
  12. Trivedi, M.K. et al. (2017). Immunomodulatory potential of nanocurcumin-based formulation. Inflammopharmacol. 25 (6): 609-619.
  13. Sapkota, M. et al. (2016). Malondialdehyde-acetaldehyde-adducted surfactant protein alters macrophage functions through Scavenger Receptor A. Alcoholism Clin. Exp. Res. 40:2563-2572.
  14. Rawat, P. & Spector, S. A. (2016). Development and characterization of a human microglia cell model of HIV-1 infection. J Neurovirol. doi:10.1007/s13365-016-0472-1.
  15. Beringer, P. M. et al. (2015). Rhesus θ-defensin-1 (RTD-1) exhibits in vitro and in vivo activity against cystic fibrosis strains of Pseudomonas aeruginosa. J Antimicrob Chemother. doi: 10.1093/jac/dkv301.
  16. Lee, S. G. et al. (2015). Immunostimulatory polysaccharide isolated from the leaves of Diospyros kaki Thumb modulate macrophage via TLR2Int J Biol Macromol. 79:971-982.
  17. Jung, J. Y. et al. (2015). Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation. Int Immunopharmacol.  doi:10.1016/j.intimp.2015.05.037.
  18. Zhang, H. et al. (2015). Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling mendelian disease. Circ Res.  doi:10.1161/CIRCRESAHA.117.305860.
  19. Fiorcari, S. et al. (2015). Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia. Haematologica. 100:253-262.
  20. Liao, W. T. et al. (2014). Cyclic GMP-dependent protein kinase II is necessary for macrophage M1 polarization and phagocytosis via toll-like receptor 2. J Mol Med (Berl). doi: 10.1007/s00109-014-1236-0.
  21. Kasat, K. et al. (2014). Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born. J Perinatol. 34:380-385.
  22. Haselow, K. et al. (2013). Bile Acids PKA-Dependently Induce a Switch of the IL-10/IL-12 Ratio and Reduce Proinflammatory Capability of Human Macrophages. J. Leukoc. Biol. 94:1253-1264.
  23. Pierce, L.M. et al. (2012). Effect Of Heavy Metal Tungsten Alloy Particles On Oxidative Product Formation And Phagocytosis By Lung Macrophages. Am. J. Respir. Crit. Care Med. 185:A4666.
  24. Polancec, D.S.et al.(2012). Azithromycin Drives in Vitro GM-CSF/IL-4-Induced Differentiation of Human Blood Monocytes Toward Dendritic-like Cells with Regulatory Properties. J Leukoc Biol. 91:229-243.

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format) CBA-222 96 assays CELL BIOLABS
CytoSelect™ 96-well Phagocytosis Assay (Red Blood Cell) CBA-220 96 assays CELL BIOLABS
CytoSelect™ 96-well Phagocytosis Assay (Zymosan) CBA-224-5 5x96 assays CELL BIOLABS
CytoSelect™ 96-well Phagocytosis Assay (Zymosan) CBA-224 96 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
CBA-222
Product
CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-220
Product
CytoSelect™ 96-well Phagocytosis Assay (Red Blood Cell)
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-224-5
Product
CytoSelect™ 96-well Phagocytosis Assay (Zymosan)
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-224
Product
CytoSelect™ 96-well Phagocytosis Assay (Zymosan)
Size
96 assays
Qty
Data Sheet
MSDS

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