시약 및 기기 상세 Phagocytosis Assay Kit Maker : CELL BIOLABS Phagocytosis Assay Kit는 면역세포의 식세포 작용(Phagocytosis) 능력을 정량적으로 측정하기 위한 kit 입니다. 프린트 제품 문의 특징 우리 몸의 면역 세포(주로 대식세포 등)는 외부의 병원체(박테리아, 이물질 등)를 잡아먹어 분해하는 '식세포 작용'을 수행합니다. 이 제품은 면역 세포가 이물질을 얼마나 효율적으로 잡아먹는지(engulfment)를 in-vitro에서 수치화하여 측정하는 키트입니다. Zymosan (효모세포벽 성분) 또는 E.coli를 pathogen으로 이용하여, 세포의 식균작용을 유도하고 분석합니다. adherent phagocytes에는 Zymosan을, suspension cell의 식균작용에는 E.coli를 이용합니다. 96-well 형식으로 제공되어 많은 샘플을 동시에 분석할 수 있습니다.Colorimetric 방법을 이용하여 Standard microplate reader로 간편하게 측정할 수 있습니다.실험 원리1. 식세포(macrophages 등)를 96-well 플레이트에 배양합니다.2. 특정 처리(예: 약물 투여 등)를 한 후, 형광이나 색깔로 표지된 위 입자들을 세포에 노출합니다.3. 세포가 입자를 잡아먹으면, 정해진 프로토콜을 통해 이를 ELISA 리더기(흡광도 측정기)로 측정합니다.적용 실험면역학 연구: 면역 세포의 활성도 측정 (면역 세포가 병원체를 얼마나 잘 잡아먹는지 평가).약물 스크리닝: 특정 약물이나 화합물이 면역 세포의 식세포 작용을 촉진하는지, 혹은 억제하는지를 분석.질병 기전 연구: 염증성 질환이나 면역 결핍 질환 등에서 식세포 기능이 어떻게 변화하는지 확인.항균제 연구: 병원체에 대한 면역 반응을 평가할 때 사용.주요 제품 Cat. No. Product Size CBA-222 CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format) 96 assays CBA-224 CytoSelect™ 96-well Phagocytosis Assay (Zymosan) 96 assays CBA-224-5 CytoSelect™ 96-well Phagocytosis Assay (Zymosan) 5 x 96 assays 사용 논문Park, E. et al. (2021). In Vivo Evaluation of Immune-Enhancing Activity of Red Gamju Fermented by Probiotic Levilactobacillus brevis KU15154 in Mice. Foods. 10(2):253. doi: 10.3390/foods10020253.Shin, J. et al. (2020). Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice. Nutrients. 12(8):E2260. doi: 10.3390/nu12082260.Vay, S.U. et al. (2020). The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function. J Neuroinflammation. 17(1):100. doi: 10.1186/s12974-020-01779-4.Zuo, P. et al. (2020). 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Cells. 8(2). pii: E173. doi: 10.3390/cells8020173.Kim, S. et al. (2019). Immune-enhancing screening of fourteen plants on murine macrophage RAW 264.7 cells. Trop J Pharm Res. 18(1): 86. doi: 10.4314/tjpr.v18i1.13.Al-Kushi, A.G. et al. (2018). Antioxidant effect of royal jelly on immune status of hyperglycemic rats. Phcog Mag. 14:528-33. doi: 10.4103/pm.pm_87_18.Vay, S.U. et al. (2018). The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo. J Neuroinflammation. 15(1):226. doi: 10.1186/s12974-018-1261-y.Trivedi, M.K. et al. (2017). Immunomodulatory potential of nanocurcumin-based formulation. Inflammopharmacol. 25 (6): 609-619.Sapkota, M. et al. (2016). Malondialdehyde-acetaldehyde-adducted surfactant protein alters macrophage functions through Scavenger Receptor A. Alcoholism Clin. Exp. Res. 40:2563-2572.Rawat, P. & Spector, S. A. (2016). Development and characterization of a human microglia cell model of HIV-1 infection. J Neurovirol. doi:10.1007/s13365-016-0472-1.Beringer, P. M. et al. (2015). Rhesus θ-defensin-1 (RTD-1) exhibits in vitro and in vivo activity against cystic fibrosis strains of Pseudomonas aeruginosa. J Antimicrob Chemother. doi: 10.1093/jac/dkv301.Lee, S. G. et al. (2015). Immunostimulatory polysaccharide isolated from the leaves of Diospyros kaki Thumb modulate macrophage via TLR2. Int J Biol Macromol. 79:971-982.Jung, J. Y. et al. (2015). Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation. Int Immunopharmacol. doi:10.1016/j.intimp.2015.05.037.Zhang, H. et al. (2015). Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling mendelian disease. Circ Res. doi:10.1161/CIRCRESAHA.117.305860.Fiorcari, S. et al. (2015). Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia. Haematologica. 100:253-262.Liao, W. T. et al. (2014). Cyclic GMP-dependent protein kinase II is necessary for macrophage M1 polarization and phagocytosis via toll-like receptor 2. J Mol Med (Berl). doi: 10.1007/s00109-014-1236-0.Kasat, K. et al. (2014). Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born. J Perinatol. 34:380-385.Haselow, K. et al. (2013). Bile Acids PKA-Dependently Induce a Switch of the IL-10/IL-12 Ratio and Reduce Proinflammatory Capability of Human Macrophages. J. Leukoc. Biol. 94:1253-1264.Pierce, L.M. et al. (2012). Effect Of Heavy Metal Tungsten Alloy Particles On Oxidative Product Formation And Phagocytosis By Lung Macrophages. Am. J. Respir. Crit. Care Med. 185:A4666.Polancec, D.S.et al.(2012). Azithromycin Drives in Vitro GM-CSF/IL-4-Induced Differentiation of Human Blood Monocytes Toward Dendritic-like Cells with Regulatory Properties. J Leukoc Biol. 91:229-243. 주문정보 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format) CBA-222 96 assays CELL BIOLABS - + 보기 보기 CytoSelect™ 96-well Phagocytosis Assay (Zymosan) CBA-224-5 5x96 assays CELL BIOLABS - + 보기 보기 CytoSelect™ 96-well Phagocytosis Assay (Zymosan) CBA-224 96 assays CELL BIOLABS - + 보기 보기 1 Maker CELL BIOLABS Cat.No. CBA-222 Product CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format) Size 96 assays Qty - + Data Sheet 보기 MSDS 보기 2 Maker CELL BIOLABS Cat.No. CBA-224-5 Product CytoSelect™ 96-well Phagocytosis Assay (Zymosan) Size 5x96 assays Qty - + Data Sheet 보기 MSDS 보기 3 Maker CELL BIOLABS Cat.No. CBA-224 Product CytoSelect™ 96-well Phagocytosis Assay (Zymosan) Size 96 assays Qty - + Data Sheet 보기 MSDS 보기 first 1 last 추천제품 혈액면역세포 | PBMCs, T cell, B cell, NK cell, Dendritic cell Cell Transformation Assay Kit ECM Cell Adhesion Assay Kit 오토파지 ELISA Kit | Autophagy ELISA Kit 세포 독성 측정 | Cytotoxicity Assay 활성산소 분석 | ROS/RNS Assay Kit [R&D Systems] Recombinant Proteins Small Molecules/Inhibitor List Cytek® Guava® easyCyte™ Flow Cytometer Flow Cytometry 워크플로우 추천제품 관련제품 견적문의 제품담기 주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다. Product Cat.No. Size Maker Qty Data Sheet MSDS 자료 웨비나/Video
혈액면역세포 | PBMCs, T cell, B cell, NK cell, Dendritic cell Cell Transformation Assay Kit ECM Cell Adhesion Assay Kit 오토파지 ELISA Kit | Autophagy ELISA Kit 세포 독성 측정 | Cytotoxicity Assay 활성산소 분석 | ROS/RNS Assay Kit [R&D Systems] Recombinant Proteins Small Molecules/Inhibitor List Cytek® Guava® easyCyte™ Flow Cytometer Flow Cytometry 워크플로우 추천제품