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세포 증식 분석 | BrdU, MTT, WST-1 Cell Proliferation Assay 이미지

세포 증식 분석 | BrdU, MTT, WST-1 Cell Proliferation Assay

BrdU, MTT, 또는 WST-1를 이용하여 세포의 증식을 분석하는 Cell Proliferation Assay Kit을 소개합니다.

프린트 제품 문의

BrdU Cell Proliferation ELISA Kit (#CBA-251)


  • BrdU(Bromodeoxyuridine)가 함유된 배지에서 세포를 키우면 DNA 합성 과정 중, Thymidine 대신 BrdU가 DNA 가닥에 통합됩니다. anti-BrdU 항체를 이용하여 BrdU를 검출합니다.
  • 발색된 색상의 흡광도 크기는 세포에 포함된 BrdU의 양에 비례하며 세포 증식과 직접적으로 연관될 수 있습니다.
  • 구성품 
    • 1000X BrdU Solution 
    • Anti-BrdU Monoclonal Antibody 
    • Fix/Denature Solution 
    • Antibody Diluent 
    • Secondary Antibody, HRP Conjugate 
    • 10X Wash Buffer 
    • Substrate Solution 
    • Stop Solution 




MTT Cell Proliferation Assay (#CBA-252)

  • 세포에 증식에 영향을 미치는 화합물로 처리하면 세포 환원요소에 의해 노란색의 tetrazole MTT가 보라색의 formazan으로 변경되고 이것을 Detection Solution으로 감지합니다. 
  • 세포가 증식하면 신호가 증가되고, 반면 신호가 감소되면 화합물이 독성을 띠는 것을 의미합니다. 
  • Cell Proliferation Reagent는 mammalian cell과 piscine cell 뿐만 아니라 bacteria, yeast, fungi, protozoa의 증식 측정에도 이용됩니다.
  • 구성품
    • MTT Cell Proliferation Assay Reagent
    • Detergent Solution



WST-1 Cell Proliferation Assay Reagent (#CBA-253)

  • 세포에 증식에 영향을 미치는 화합물로 처리하면 세포는 NADH와 전자매개체의 존재하에 살아있는 세포에서 WST-1이 formazan으로 변경되는 것을 측정합니다.
  • 세포가 증식하면 신호가 증가되고, 반면 신호가 감소되면 화합물이 독성을 띠는 것을 의미합니다. 
  • Cell Proliferation Reagent는 mammalian cell과 piscine cell 뿐만 아니라 bacteria, yeast, fungi, protozoa의 증식 측정에도 이용됩니다.
  • 구성품
    • WST-1 Cell Proliferation Assay Reagent




사용 논문

CBA-251 　
  1. Houri, A. et al. (2023). Suprabasin enhances the invasion, migration, and angiogenic ability of oral squamous cell carcinoma cells under hypoxic conditions. Oncol Rep. 49(5):83. doi: 10.3892/or.2023.8520.
  2. Lee, H. et al. (2022). Biological Effects of Yeast-fermented Plant Root Extract Mixture on Skin Cells.  Asian J Beauty Cosmetol. 20(3): 349-359. doi: 10.20402/ajbc.2022.0045.
  3. Li, L. et al. (2022). Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev. doi: 10.1155/2022/9366494.
  4. Fujino, T. (2022). Transient Receptor Potential Melastatin 8, a sensor of cold temperatures mediates expression of cyclin-dependent kinase inhibitor, p21/Cip1, a regulator of epidermal cell proliferation. J Toxicol Sci. 47(3):117-123. doi: 10.2131/jts.47.117.
  5. Fan, L. et al. (2021). MicroRNA miR-23b-3p promotes osteosarcoma by targeting ventricular zone expressed PH domain-containing 1 (VEPH1)/phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. Bioengineered. 12(2):12568-12582. doi: 10.1080/21655979.2021.2010383.
  6. Kwon, S.Y. et al. (2021). β2‑adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes. Oncol Lett. doi: 10.3892/ol.2021.13065.
  7. Soldan, S.S. et al. (2021). EBNA1 inhibitors have potent and selective antitumor activity in xenograft models of Epstein-Barr virus-associated gastric cancer. Gastric Cancer. doi: 10.1007/s10120-021-01193-6.
  8. Wu, Q. et al. (2021). Tumor suppressor role of sFRP‑4 in hepatocellular carcinoma via the Wnt/β‑catenin signaling pathway. Mol Med Rep. 23(5):336. doi: 10.3892/mmr.2021.11975.
  9. Blasetti Fantauzzi, C. et al. (2020). Galectin-3 gene deletion results in defective adipose tissue maturation and impaired insulin sensitivity and glucose homeostasis. Sci Rep. 10(1):20070. doi: 10.1038/s41598-020-76952-z.
  10. Nguyen, U.T. et al. (2020). Natural Compound Mixture, Containing Emodin, Genipin, Chlorogenic Acid, Cimigenoside, and Ginsenoside Rb1, Ameliorates Psoriasis-Like Skin Lesions by Suppressing Inflammation and Proliferation in Keratinocytes. Evid Based Complement Alternat Med. doi: 10.1155/2020/9416962.
  11. Terazawa, S. et al. (2020). Mycosporine-like amino acids stimulate hyaluronan secretion by up-regulating hyaluronan synthase 2 via activation of the p38/MSK1/CREB/c‑Fos/AP-1 axis. J Biol Chem. pii: jbc.RA119.011139. doi: 10.1074/jbc.RA119.011139.
  12. Abo-El Fetoh, M.E. et al. (2020). Cyclosporin A activates human hepatocellular carcinoma (HepG2 cells) proliferation: implication of EGFR-mediated ERK1/2 signaling pathway. Naunyn Schmiedebergs Arch Pharmacol. doi: 10.1007/s00210-019-01798-w.
  13. Cebrián, R. et al. (2019). Preclinical studies of toxicity and safety of the AS-48 bacteriocin. Journal of Advanced Research. doi: 10.1016/j.jare.2019.06.003.
  14. Dhar, S.K. et al. (2019). UVB-induced inactivation of manganese-containing superoxide dismutase promotes mitophagy via ROS-mediated mTORC2 pathway activation. J Biol Chem. pii: jbc.RA118.006595. doi: 10.1074/jbc.RA118.006595.
  15. Messick, T.E. et al. (2019). Structure-based design of small-molecule inhibitors of EBNA1 DNA binding blocks Epstein-Barr virus latent infection and tumor growth. Sci Transl Med. 11(482). pii: eaau5612. doi: 10.1126/scitranslmed.aau5612.
  16. Nguyen, L.T.H. et al. (2019). Astragali Radix and its compound formononetin ameliorate diesel particulate matter-induced skin barrier disruption by regulation of keratinocyte proliferation and apoptosis. J Ethnopharmacol. 228:132-141. doi: 10.1016/j.jep.2018.09.025.
  17. Ha, Y.J. et al. (2019). PIM-1 kinase is a novel regulator of proinflammatory cytokine-mediated responses in rheumatoid arthritis fibroblast-like synoviocytes. Rheumatology (Oxford). 58(1):154-164. doi: 10.1093/rheumatology/key261.
  18. Chen, B. et al. (2018). Co-expression of Akt1 and Wnt11 promotes the proliferation and cardiac differentiation of mesenchymal stem cells and attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Biomed Pharmacother. 108:508-514. doi: 10.1016/j.biopha.2018.09.047.
  19. Yoon, J.E. et al. (2018). Senescent fibroblasts drive ageing pigmentation: ​A potential therapeutic target for senile lentigo. Theranostics. 8(17):4620-4632. doi: 10.7150/thno.26975.
  20. Bautista, W. W. et al. (2018). Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. Journal of Clinical and Experimental Hepatology. doi: 10.1016/j.jceh.2018.02.002.
  21. Beretti, F. et al. (2018). Amniotic fluid stem cell exosomes: Therapeutic perspective. Biofactors. 44(2):158-167. doi: 10.1002/biof.1407.
  22. Maglione, M. et al. (2017). In vivo evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration. J. Oral Sci. 59(2):225-232.
  23. Kim, B. S. et al. (2016). D-dopachrome tautomerase in adipose tissue inflammation and wound repair. J Cell Mol Med. doi:10.1111/jcmm.12936.
  24. Kreiseder, B. et al. (2015). Alpha-catulin contributes to drug-resistance of melanoma by activating NF-κB and AP-1. PLoS One. 10:e0119402-e0119402.
  25. Hatzis, C. et al. (2014) Enhancing Reproducibility in Cancer Drug Screening: How Do We Move Forward? Cancer Res. 74:4016-4023.
CBA-252 　
  1. Houri, A. et al. (2023). Suprabasin enhances the invasion, migration, and angiogenic ability of oral squamous cell carcinoma cells under hypoxic conditions. Oncol Rep. 49(5):83. doi: 10.3892/or.2023.8520.
  2. Lee, H. et al. (2022). Biological Effects of Yeast-fermented Plant Root Extract Mixture on Skin Cells.  Asian J Beauty Cosmetol. 20(3): 349-359. doi: 10.20402/ajbc.2022.0045.
  3. Li, L. et al. (2022). Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev. doi: 10.1155/2022/9366494.
  4. Fujino, T. (2022). Transient Receptor Potential Melastatin 8, a sensor of cold temperatures mediates expression of cyclin-dependent kinase inhibitor, p21/Cip1, a regulator of epidermal cell proliferation. J Toxicol Sci. 47(3):117-123. doi: 10.2131/jts.47.117.
  5. Fan, L. et al. (2021). MicroRNA miR-23b-3p promotes osteosarcoma by targeting ventricular zone expressed PH domain-containing 1 (VEPH1)/phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. Bioengineered. 12(2):12568-12582. doi: 10.1080/21655979.2021.2010383.
  6. Kwon, S.Y. et al. (2021). β2‑adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes. Oncol Lett. doi: 10.3892/ol.2021.13065.
  7. Soldan, S.S. et al. (2021). EBNA1 inhibitors have potent and selective antitumor activity in xenograft models of Epstein-Barr virus-associated gastric cancer. Gastric Cancer. doi: 10.1007/s10120-021-01193-6.
  8. Wu, Q. et al. (2021). Tumor suppressor role of sFRP‑4 in hepatocellular carcinoma via the Wnt/β‑catenin signaling pathway. Mol Med Rep. 23(5):336. doi: 10.3892/mmr.2021.11975.
  9. Blasetti Fantauzzi, C. et al. (2020). Galectin-3 gene deletion results in defective adipose tissue maturation and impaired insulin sensitivity and glucose homeostasis. Sci Rep. 10(1):20070. doi: 10.1038/s41598-020-76952-z.
  10. Nguyen, U.T. et al. (2020). Natural Compound Mixture, Containing Emodin, Genipin, Chlorogenic Acid, Cimigenoside, and Ginsenoside Rb1, Ameliorates Psoriasis-Like Skin Lesions by Suppressing Inflammation and Proliferation in Keratinocytes. Evid Based Complement Alternat Med. doi: 10.1155/2020/9416962.
  11. Terazawa, S. et al. (2020). Mycosporine-like amino acids stimulate hyaluronan secretion by up-regulating hyaluronan synthase 2 via activation of the p38/MSK1/CREB/c‑Fos/AP-1 axis. J Biol Chem. pii: jbc.RA119.011139. doi: 10.1074/jbc.RA119.011139.
  12. Abo-El Fetoh, M.E. et al. (2020). Cyclosporin A activates human hepatocellular carcinoma (HepG2 cells) proliferation: implication of EGFR-mediated ERK1/2 signaling pathway. Naunyn Schmiedebergs Arch Pharmacol. doi: 10.1007/s00210-019-01798-w.
  13. Cebrián, R. et al. (2019). Preclinical studies of toxicity and safety of the AS-48 bacteriocin. Journal of Advanced Research. doi: 10.1016/j.jare.2019.06.003.
  14. Dhar, S.K. et al. (2019). UVB-induced inactivation of manganese-containing superoxide dismutase promotes mitophagy via ROS-mediated mTORC2 pathway activation. J Biol Chem. pii: jbc.RA118.006595. doi: 10.1074/jbc.RA118.006595.
  15. Messick, T.E. et al. (2019). Structure-based design of small-molecule inhibitors of EBNA1 DNA binding blocks Epstein-Barr virus latent infection and tumor growth. Sci Transl Med. 11(482). pii: eaau5612. doi: 10.1126/scitranslmed.aau5612.
  16. Nguyen, L.T.H. et al. (2019). Astragali Radix and its compound formononetin ameliorate diesel particulate matter-induced skin barrier disruption by regulation of keratinocyte proliferation and apoptosis. J Ethnopharmacol. 228:132-141. doi: 10.1016/j.jep.2018.09.025.
  17. Ha, Y.J. et al. (2019). PIM-1 kinase is a novel regulator of proinflammatory cytokine-mediated responses in rheumatoid arthritis fibroblast-like synoviocytes. Rheumatology (Oxford). 58(1):154-164. doi: 10.1093/rheumatology/key261.
  18. Chen, B. et al. (2018). Co-expression of Akt1 and Wnt11 promotes the proliferation and cardiac differentiation of mesenchymal stem cells and attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Biomed Pharmacother. 108:508-514. doi: 10.1016/j.biopha.2018.09.047.
  19. Yoon, J.E. et al. (2018). Senescent fibroblasts drive ageing pigmentation: ​A potential therapeutic target for senile lentigo. Theranostics. 8(17):4620-4632. doi: 10.7150/thno.26975.
  20. Bautista, W. W. et al. (2018). Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. Journal of Clinical and Experimental Hepatology. doi: 10.1016/j.jceh.2018.02.002.
  21. Beretti, F. et al. (2018). Amniotic fluid stem cell exosomes: Therapeutic perspective. Biofactors. 44(2):158-167. doi: 10.1002/biof.1407.
  22. Maglione, M. et al. (2017). In vivo evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration. J. Oral Sci. 59(2):225-232.
  23. Kim, B. S. et al. (2016). D-dopachrome tautomerase in adipose tissue inflammation and wound repair. J Cell Mol Med. doi:10.1111/jcmm.12936.
  24. Kreiseder, B. et al. (2015). Alpha-catulin contributes to drug-resistance of melanoma by activating NF-κB and AP-1. PLoS One. 10:e0119402-e0119402.
  25. Hatzis, C. et al. (2014) Enhancing Reproducibility in Cancer Drug Screening: How Do We Move Forward? Cancer Res. 74:4016-4023.
CBA-253 　
  1. Durmaz, B. et al. (2021). Antileukemic Effects of Anti-miR-146a, Anti-miR-155, Anti-miR-181a, and Prednisolone on Childhood Acute Lymphoblastic Leukemia. Biomed Res Int. doi: 10.1155/2021/3207328.
  2. Menini, S. et al. (2020). Diabetes promotes invasive pancreatic cancer by increasing systemic and tumour carbonyl stress in KrasG12D/+ mice. J Exp Clin Cancer Res. 39(1):152. doi: 10.1186/s13046-020-01665-0.
  3. Harada, H. et al. (2020). Prediction of Efficacy of Postoperative Chemotherapy by DNA Methylation of CDO1 in Gastric Cancer. J Surg Res. 256:404-412. doi: 10.1016/j.jss.2020.07.001.
  4. Yokota, K. et al. (2019). WiNTRLINC1/ASCL2/c-Myc Axis Characteristics of Colon Cancer with Differentiated Histology at Young Onset and Essential for Cell Viability. Ann Surg Oncol. doi: 10.1245/s10434-019-07780-3.
  5. Harada, H. et al. (2019). Cancer-specific promoter DNA methylation of Cysteine dioxygenase type 1 (CDO1) gene as an important prognostic biomarker of gastric cancer. PLoS One. 14(4):e0214872. doi: 10.1371/journal.pone.0214872.
  6. Woo, H.H. et al. (2019). Human ALKBH3-induced m1A demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells. Biochim Biophys Acta Gene Regul Mech. 1862(1):35-46. doi: 10.1016/j.bbagrm.2018.10.008.
  7. Yang, Q. et al. (2018). PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nat Commun. 9(1):4667. doi: 10.1038/s41467-018-07132-x.
  8. Yokoi, K. et al. (2018). Epigenetic Status of CDO1 Gene May Reflect Chemosensitivity in Colon Cancer with Postoperative Adjuvant Chemotherapy. Ann Surg Oncol. 26(2):406-414. doi: 10.1245/s10434-018-6865-z.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
BrdU Cell Proliferation In-cell ELISA Kit ARG82214 96 assay ARIGO
CytoSelect™ BrdU Cell Proliferation ELISA Kit CBA-251 96 assays CELL BIOLABS
CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric) CBA-253 10 ml CELL BIOLABS
CytoSelect™ MTT Cell Proliferation Assay CBA-252 960 assays CELL BIOLABS
WST-1 Cell Proliferation Assay Reagent ARG82216 960 assay ARIGO
Maker
ARIGO
Cat.No.
ARG82214
Product
BrdU Cell Proliferation In-cell ELISA Kit
Size
96 assay
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
CBA-251
Product
CytoSelect™ BrdU Cell Proliferation ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-253
Product
CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)
Size
10 ml
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
CBA-252
Product
CytoSelect™ MTT Cell Proliferation Assay
Size
960 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82216
Product
WST-1 Cell Proliferation Assay Reagent
Size
960 assay
Qty
Data Sheet

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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