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제품 상세
In Vitro Angiogenesis Assay | Endothelial Tube Formation Assay 이미지

In Vitro Angiogenesis Assay | Endothelial Tube Formation Assay

EHS(Engelbreth–Holm–Swarm) tumor cell 유래의 ECM을 이용하여 내피세포의 tube 형성능을 확인할 수 있도록 고안된 Assay Kit입니다.

프린트 제품 문의

특징


  • Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay) kit를 이용하여 In vitro에서 angiogenic tube의 형성을 관찰할 수 있습니다.
  • In vivo basement membrane 환경과 비슷한 ECM matrix gel을 이용합니다.
  • 18시간 내에 tube formation을 관찰할 수 있습니다.
  • kit에 제공되는 형광 dye를 통해 tube 길이, branch point등의 tube formation 정도를 확인할 수 있습니다.
  • Size : 50 assays
  • Detection : Light Microscopy


Kit의 구성

  • ECM Gel Solution
  • 10X Staining Buffer 
  • Staining Dye



               HUVEC Tube Formation on ECM Gel. Left: HUVEC cells on tissue culture plate. Right: Tube formation on ECM Gel.




사용 논문

CBA-200 　
    1. Rofia, B. et al. (2021). Regulatory interplay between Vav1, Syk and β-catenin occurs in lung cancer cells. Cell Signal. doi: 10.1016/j.cellsig.2021.110079.
    2. Gurel Pekozer, G. et al. (2021). Investigation of Vasculogenesis Inducing Biphasic Scaffolds for Bone Tissue Engineering. ACS Biomater Sci Eng. doi: 10.1021/acsbiomaterials.0c01071. 
    3. Zuo, Q. et al. (2021). Aspirin reduces sFlt-1 mediated apoptosis of trophoblast cells in preeclampsia. Mol Hum Reprod. doi: 10.1093/molehr/gaaa089.
    4. Javadi, J. et al. (2021). Syndecan-1 Overexpressing Mesothelioma Cells Inhibit Proliferation, Wound Healing, and Tube Formation of Endothelial Cells. Cancers (Basel). 13(4):655. doi: 10.3390/cancers13040655.
    5. Wolcott, K.M. et al. (2020). CD34 positive cells isolated from traumatized human skeletal muscle require the CD34 protein for multi-potential differentiation. Cell Signal. doi: 10.1016/j.cellsig.2020.109711.
    6. Guerrero, F. et al. (2020). Role of endothelial microvesicles released by p-cresol on endothelial dysfunction. Sci Rep. 10(1):10657. doi: 10.1038/s41598-020-67574-6.
    7. Kim, H.K. et al. (2019). RRAD expression in gastric and colorectal cancer with peritoneal carcinomatosis. Sci Rep. 9(1):19439. doi: 10.1038/s41598-019-55767-7.
    8. Cam, M. et al. (2016). ΔNp63 mediates cellular survival and metastasis in canine osteosarcoma. Oncotarget. 7(30):48533-48546. doi: 10.18632/oncotarget.10406.
    9. Shirasu, N. et al. (2019). Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies. Cancer Cell Int. doi: 10.1186/s12935-019-1034-4.
    10. Kitala, D. et al. (2019). Amniotic cells share clusters of differentiation of fibroblasts and keratinocytes, influencing their ability to proliferate and aid in wound healing while impairing their angiogenesis capability. Eur J Pharmacol. pii: S0014-2999(19)30148-7. doi: 10.1016/j.ejphar.2019.02.043.
    11. Teng, X. et al. (2019). Selective deletion of endothelial cell calpain in mice reduces diabetic cardiomyopathy by improving angiogenesis. Diabetologia. 62(5):860-872. doi: 10.1007/s00125-019-4828-y.
    12. Li, W. et al. (2019). LncRNA OR3A4 participates in the angiogenesis of hepatocellular carcinoma through modulating AGGF1/akt/mTOR pathway. Eur J Pharmacol. 849:106-114. doi: 10.1016/j.ejphar.2019.01.049.
    13. Chen, C.Y. et al. (2018). N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration. Matrix Biol. 70:84-101. doi: 10.1016/j.matbio.2018.03.013.
    14. Mouritzen, M.V. et al. (2018). Neurotensin, substance P, and insulin enhance cell migration. J Pept Sci. 24(7):e3093. doi: 10.1002/psc.3093.
    15. Kudo, H. et al. (2018). A potential role for the silent information regulator 2 homologue 1 (SIRT1) in periapical periodontitis. Int Endod J. 51(7):747-757. doi: 10.1111/iej.12894.
    16. Suda, M. et al. (2017). Inhibition of dipeptidyl peptidase-4 ameliorates cardiac ischemia and systolic dysfunction by up-regulating the FGF-2/EGR-1 pathway. PLoS One. 12(8):e0182422. doi: 10.1371/journal.pone.0182422.
    17. Bae, W.J. et al. (2017). Lysyl oxidase-mediated VEGF-induced differentiation and angiogenesis in human dental pulp cells. Int. Endod. J. doi: 10.1111/iej.12796.
    18. Sakaguchi, K. et al. (2017). Periodontal tissue regeneration using the cytokine cocktail mimicking secretomes in the conditioned media from human mesenchymal stem cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.01.065.
    19. Katagiri, W. et al. (2017). A defined mix of cytokines mimics conditioned medium from cultures of bone marrow-derived mesenchymal stem cells and elicits bone regeneration. Cell Prolif. doi: 10.1111/cpr.12333.
    20. Kim, J.Y. et al. (2017). Role of Protein Phosphatase 1 in Angiogenesis and Odontoblastic Differentiation of Human Dental Pulp Cells. J Endod. doi: 10.1016/j.joen.2016.10.013.
    21. Lee, S.I. et al. (2016). Baicalein promotes angiogenesis and odontoblastic differentiation via the BMP and Wnt pathways in human dental pulp cells. Am. J. Chinese Med. 44:1457.
    22. Yun, H.M. et al. (2016). Magnetic nanofiber scaffold-induced stimulation of odontogenesis and pro-angiogenesis of human dental pulp cells through Wnt/MAPK/NF-κB pathways. Dent Mater. doi:10.1016/j.dental.2016.06.016.
    23. Qiu, D. et al. (2016). Overexpression of FoxP1 is a novel biomarker of malignant human pancreatic cancer. Int J Clin Exp Med. 9:9054-9063.
    24. Pekozer, G. G. et al. (2016). Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells. Microvasc Res. doi:10.1016/j.mvr.2016.06.005.
    25. Sun, Y. et al. (2016). Toll-like receptor 4 promotes angiogenesis in pancreatic cancer via PI3K/AKT signaling. Exp Cell Res. doi:10.1016/j.yexcr.2016.07.009.
    26. Bid, H. K. et al. (2016). The Bromodomain BET inhibitor JQ1 suppresses tumor angiogenesis in models of childhood sarcoma. Mol Cancer Ther. doi:10.1158/1535-7163.
    27. Yun, H. M. et al. (2016). Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation. Biomaterials. 85:88-98.
    28. Chang, S. W. et al. (2016). Combined effects of mineral trioxide aggregate and human placental extract on rat pulp tissue and growth, differentiation and angiogenesis in human dental pulp cells. Acta Odontol Scand. doi:10.3109/00016357.2015.1120882.
    29. Sun, T. et al. (2016). Forkhead box protein k1 recruits TET1 to act as a tumor suppressor and is associated with MRI detection. Jpn J Clin Oncol. doi:10.1093/jjco/hyv185.
    30. Al-Mahrouki, A. A. et al. (2015). Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism.Oncoscience.2:944-957.


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay) CBA-200 50 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
CBA-200
Product
Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)
Size
50 assays
Qty
Data Sheet
MSDS

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

  • Cancer Research Product Guide

    Cancer Research Product Guide 이미지

    [TOCRIS]

    Cancer 연구에 관련된 small molecule 정보가 수록되어 있습니다.

  • Tumor microenvironment (TME)

    Tumor microenvironment (TME) 이미지

    [ARIGO]

    종양미세환경과 관련된 marker와 pathway에 대한 포스터 입니다.

웨비나/Video

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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