고마바이오텍(주)

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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

Beta-Galactosidase Assay (#AKR-100) 

  

  • LacZ gene의 발현과 transfection 효율을 간편하게 확인하는 방법입니다.
  • Transfected gene이 발현된 세포(lacZ 발현)에서 ß-Galactosidase에 의해 X-gal의 분해가 일어나고, 이로 인해 파란색을 띄게 됩니다.
  • 염색방법이 간단하며, 35mm dishes 기준으로 75 Assay를 진행할 수 있는 충분한 양의 시약을 제공합니다.

 

Beta-Galactosidase Assay (#AKR-100)
X-gal staining of infected HUVEC cells and chick CAM tissue. A.
HUVEC cells were infected with purified Ad-β Gal at 50 MOI (multiplicity of infection). X-gal staining was performed after 48 hr infection period. B. Purified Ad- β Gal was injected intravenously into a 10-day old chick embryo, after three days, X-gal staining was performed on the CAM (chick chorioallanoic membrane) tissue.

  

 

 

GFP ELISA Kit (#AKR-121) 

  

  • 세포 또는 조직 샘플 내 GFP(Green fluorescent protein) 및 GFP fusion protein을 정량합니다.
  • ELISA 방법으로 GFP, BFP, CFP, YFP (from Aequorea victoria)를 측정합니다. 
  • Detection sensitivity : ~ 30pg/ml

 

 

 

GFP Quantitation Kit (#AKR-120)


  • 세포 또는 조직 샘플 내 GFP(Green fluorescent protein) 및 GFP fusion protein을 정량합니다.
  • 형광으로 측정하여 감도가 높습니다. 
  • Detection sensitivity : ~ 100ng/ml

 

 

 

RFP ELISA Kit (#AKR-122)

 

  • 세포 또는 조직 샘플 내 RFP(Red fluorescent protein) 및 RFP fusion protein의 양을 측정합니다.
  • TagRFP, TurboRFP, DsRed, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, mStrawberry, tdTomato 등 변형된 형태의 Protein과도 반응합니다.
  • FACS 분석보다 빠르고 사용이 편리합니다.
  • Detection sensitivity : ~ 150 pg/ml

 

 

 

 

사용 논문

 

AKR-100 　
    1. Kadoya, H. et al. (2020). Klotho is a novel therapeutic target in peritoneal fibrosis via Wnt signaling inhibition. Nephrol Dial Transplant. pii: gfz298. doi: 10.1093/ndt/gfz298.
    2. Rani, S. et al. (2017). Senescence in the lesional fibroblasts of non-segmental vitiligo patients. Arch Dermatol Res. doi: 10.1007/s00403-016-1713-0.
    3. Ge, X. et al. (2015). Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis. 5:28776.
    4. Xu, X. et al. (2015). Aberrant activation of TGF‐β in subchondral bone at the onset of rheumatoid arthritis joint destruction.  J Bone Miner Res. doi: 10.1002/jbmr.2550.
    5. Black, S.A. et al. (2008). TGFß1 stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGFß1-induced CCN2/CTGF expression. J. Biol. Chem. 283:10835-10847.
AKR-121 　
    1. Czajka, M. et al. (2020). Mosaic Recombinant Adeno-associated Virus Vector rAAV/DJ/CAG for Targeted Gene Delivery to Melanoma Cells Metastasized to the Lung. Anticancer Res. 40(8):4425-4444. doi: 10.21873/anticanres.14448.
    2. Rodier, J.T. et al. (2019). Linear Polyethylenimine-DNA Nanoconstruct for Corneal Gene Delivery. J Ocul Pharmacol Ther. 35(1):23-31. doi: 10.1089/jop.2018.0024.
    3. Kwon, K.C. et al. (2019). An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP). Fish Shellfish Immunol. 87:414-420. doi: 10.1016/j.fsi.2019.01.038.
    4. Yang, B. et al. (2018). Comparative studies of the serum half-life extension of a protein via site-specific conjugation to a species-matched or -mismatched albumin. Biomater Sci. 6(8):2092-2100. doi: 10.1039/c8bm00456k.
    5. Obajemu, A.A. et al. (2017). IFN-λ4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling. J Immunol. 199(11):3808-3820. doi: 10.4049/jimmunol.1700807.
    6. Shrestha, R.P. et al (2017). Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica. Microb Cell Fact. 16(1):145. doi: 10.1186/s12934-017-0760-3.
    7. Fowler, K.A. et al. (2017). Targeting the Canonical Nuclear Factor-κB Pathway with a High-Potency IKK2 Inhibitor Improves Outcomes in a Mouse Model of Idiopathic Pneumonia Syndrome. Biol Blood Marrow Transplant. doi: 10.1016/j.bbmt.2017.01.083.
    8. Gordon, E. D. et al. (2016). Alternative splicing of interleukin-33 and type 2 inflammation in asthma. Proc Natl Acad Sci U S A. 113:8765-8770.
    9. Yang, B. et al. (2016). Site-specific albumination as an alternative to PEGylation for the enhanced serum half-life in vivo. Biomacromolecules. doi:10.1021/acs.biomac.6b00238.
    10. Vance, M. et al. (2016). AAV gene therapy for MPS1-associated corneal blindness. Sci Rep. doi:10.1038/srep22131.
    11. Johnson, K. A. et al. (2016). The Ebola Virus matrix protein, VP40, requires phosphatidylinositol 4, 5-bisphosphate (PI (4, 5) P2) for extensive oligomerization at the plasma membrane and viral egress.  Sci Rep. 6:19125.
    12. Chen, Z. et al. (2016). GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway. Cell Death Dis. doi:10.1038/cddis.2015.300.
    13. Chen, C. C. et al. (2015). Changes in DNA methylation are associated with the development of drug resistance in cervical cancer cells. Cancer Cell Int. 15:98.
    14. Borjan, B. et al. (2015). The Aplidin analogs PM01215 and PM02781 inhibit angiogenesis in vitro and in vivo. BMC Cancer. 15:738.
    15. Gee, H. Y. et al. (2015). KANK deficiency leads to podocyte dysfunction and nephrotic syndrome. J Clin Invest. doi: 10.1172/JCI79504. 
    16. Vemula, S. V. et al. (2015). HIV-1 induced Nuclear Factor IB (NF-IB) expression negatively regulates HIV-1 replication through interaction with the long terminal repeat region. Viruses.  7:543-558.
    17. Zhang, Y. et al. (2015). Characterization of the promoter of Grapevine vein clearing virus. J Gen Virol. 96:165-169.
    18. Ott, L. & Carson, S. (2014). Immunological tools: Engaging students in the use and analysis of flow cytometry and enzyme‐linked immunosorbent assay (ELISA). Biochem Mol Biol Educ.  42:382-397.
    19. Fulton, L. M. et al. (2014). Altered T-cell entry and egress in the absence of Coronin 1A attenuates murine acute graft versus host disease.Eur J Immunol. 44:1662-1671.
    20. Baez, A. et al. (2014).  Production of recombinant protein by a novel oxygen-induced system in Escherichia coli. Microb Cell Fact. 13:50.
    21. Huhtala, T. et al. (2014). Biodistribution and antitumor effect of Cetuximab-targeted lentivirus. Nucl Med Biol. 41:77-83.
    22. Anyaegbu, C.  C. et al. (2014). Chemotherapy enhances cross-presentation of nuclear tumor antigens. PLoS One.  9:e107894.
    23. Sendra, L. et al. (2014). Low RNA translation activit limits the efficacy of hydrodynamic gene transfer to pig liver in vivo. J Gene Med. 16:179-192.
    24. Mango, R. et al. (2014). C-C Chemokine Receptor 5 on Pulmonary Mesenchymal Cells Promotes Experimental Metastasis via the Induction of Erythroid Differentiation Regulator 1. Mol. Cancer. Res. 12:274-282.
    25. Mitchell, A. et al. (2014). Promyelocytic Leukemia Protein Is a Cell-Intrinsic Factor Inhibiting Parvovirus DNA Replication. J. Virol. 88:925-936.
    26. Coghill, J.M. et al. (2013). CC chemokine Receptor 8 Potentiates Donor Treg Survival and is Critical for the Prevention of Murine Graft-Versus-Host Disease. Blood. 122:825-836.
    27. Pedersen, J. et al. (2012). Glucose Metabolism is Altered After Loss of L Cells and α-cells but Not Influenced by Loss of K Cells. Am J Physiol Endocrinol Metab. 304:E60-E73.
    28. Zhou, W. et al. (2012).Inducible Podocyte Injury and Proteinuria in Transgenic Zebrafish. J. Am. Soc. Nephrol. 23:1039-1047.
    29. Fonseca, J.P. et al. (2012). In Vivo Polycomb Kinetics and Mitotic Chromatin Binding Distinguish Stem Cells from Differentiated Cells. Genes and Dev. 26:857-871.
    30. Rajan, S. et al. (2010). In Vitro Processing and Secretion of Mutant Insulin Proteins that Cause Permanent Neonatal Diabetes. Am. J. Physiol. Endocrinol. Metab. 298:E403-E410.
AKR-120 　
    1. Occhialini, A. et al. (2020). Generation, analysis, and transformation of macro-chloroplast Potato (Solanum tuberosum) lines for chloroplast biotechnology. Sci Rep. 10(1):21144. doi: 10.1038/s41598-020-78237-x.
    2. Ahn, G. et al. (2019). Controlling the Release Profile Through Phase Control of Calcium Phosphate-Alginate Core-shell Nanoparticles in Gene Delivery. Macromolecular Research. doi: 10.1007/s13233-019-7106-4.
    3. Liu, J. et al. (2019). Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry. Sci Rep. 8(1):13442. doi: 10.1038/s41598-018-31713-x.
    4. Nichols, N. N. et al. (2018). Use of green fluorescent protein to monitor fungal growth in biomass hydrolysate. Biology Methods and Protocols. 3(1). doi: 10.1093/biomethods/bpx012.
    5. Lipták, N. et al. (2018). Glomerulosclerosis in transgenic rabbits with ubiquitous Venus protein expression. Acta Vet Hung. 66(2):281-293. doi: 10.1556/004.2018.026.
    6. Shim, M. S. et al. (2014). Stimuli-responsive siRNA carriers for efficient gene silencing in tumors via systemic delivery. Biomater Sci. 2:35-40.
    7. Pfeiffer, B. et al. (2012). Using Translational Enhancers to Increase Transgene Expression in Drosophila. PNAS. 109:6626-6631.(#AKR-120)
    8. Wamboldt, Y. et al. (2009). Participation of Leaky Ribosome Scanning in Protein Dual Targeting by Alternative Translation Initiation in Higher Plants. Plant Cell 10.1105/tpc.108.063644. (#AKR-120)
AKR-122

Fang, J. et al. (2015). COPII dependent ER export: a critical component of insulin biogenesis and beta cell ER homeostasis. Mol Endocrinol. doi:10.1210/me.2015-1012.

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
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관련제품

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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