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제품 상세
ß-Galactosidase Reporter Assay Kit | GFP/RFP Assay Kit 이미지

ß-Galactosidase Reporter Assay Kit | GFP/RFP Assay Kit

유전자의 기능 및 발현조절 연구에 사용되는 GFP (Green fluorescent protein), RFP (Red fluorescent protein), beta-galactosidase Assay kit을 소개합니다.

프린트 제품 문의

Beta-Galactosidase Assay (#AKR-100) 

  

  • LacZ gene의 발현과 transfection 효율을 간편하게 확인하는 방법입니다.
  • Transfected gene이 발현된 세포(lacZ 발현)에서 ß-Galactosidase에 의해 X-gal의 분해가 일어나고, 이로 인해 파란색을 띄게 됩니다.
  • 염색방법이 간단하며, 35mm dishes 기준으로 75 Assay를 진행할 수 있는 충분한 양의 시약을 제공합니다.

 

Beta-Galactosidase Assay (#AKR-100)
X-gal staining of infected HUVEC cells and chick CAM tissue. A.
HUVEC cells were infected with purified Ad-β Gal at 50 MOI (multiplicity of infection). X-gal staining was performed after 48 hr infection period. B. Purified Ad- β Gal was injected intravenously into a 10-day old chick embryo, after three days, X-gal staining was performed on the CAM (chick chorioallanoic membrane) tissue.

  

 

 

GFP ELISA Kit (#AKR-121) 

  

  • 세포 또는 조직 샘플 내 GFP(Green fluorescent protein) 및 GFP fusion protein을 정량합니다.
  • ELISA 방법으로 GFP, BFP, CFP, YFP (from Aequorea victoria)를 측정합니다. 
  • Detection sensitivity : ~ 30pg/ml

 

 

 

GFP Quantitation Kit (#AKR-120)


  • 세포 또는 조직 샘플 내 GFP(Green fluorescent protein) 및 GFP fusion protein을 정량합니다.
  • 형광으로 측정하여 감도가 높습니다. 
  • Detection sensitivity : ~ 100ng/ml

 

 

 

RFP ELISA Kit (#AKR-122)

 

  • 세포 또는 조직 샘플 내 RFP(Red fluorescent protein) 및 RFP fusion protein의 양을 측정합니다.
  • TagRFP, TurboRFP, DsRed, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, mStrawberry, tdTomato 등 변형된 형태의 Protein과도 반응합니다.
  • FACS 분석보다 빠르고 사용이 편리합니다.
  • Detection sensitivity : ~ 150 pg/ml

 

 

 

 

사용 논문

 

AKR-100 　
    1. Kadoya, H. et al. (2020). Klotho is a novel therapeutic target in peritoneal fibrosis via Wnt signaling inhibition. Nephrol Dial Transplant. pii: gfz298. doi: 10.1093/ndt/gfz298.
    2. Rani, S. et al. (2017). Senescence in the lesional fibroblasts of non-segmental vitiligo patients. Arch Dermatol Res. doi: 10.1007/s00403-016-1713-0.
    3. Ge, X. et al. (2015). Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis. 5:28776.
    4. Xu, X. et al. (2015). Aberrant activation of TGF‐β in subchondral bone at the onset of rheumatoid arthritis joint destruction.  J Bone Miner Res. doi: 10.1002/jbmr.2550.
    5. Black, S.A. et al. (2008). TGFß1 stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGFß1-induced CCN2/CTGF expression. J. Biol. Chem. 283:10835-10847.
AKR-121 　
    1. Czajka, M. et al. (2020). Mosaic Recombinant Adeno-associated Virus Vector rAAV/DJ/CAG for Targeted Gene Delivery to Melanoma Cells Metastasized to the Lung. Anticancer Res. 40(8):4425-4444. doi: 10.21873/anticanres.14448.
    2. Rodier, J.T. et al. (2019). Linear Polyethylenimine-DNA Nanoconstruct for Corneal Gene Delivery. J Ocul Pharmacol Ther. 35(1):23-31. doi: 10.1089/jop.2018.0024.
    3. Kwon, K.C. et al. (2019). An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP). Fish Shellfish Immunol. 87:414-420. doi: 10.1016/j.fsi.2019.01.038.
    4. Yang, B. et al. (2018). Comparative studies of the serum half-life extension of a protein via site-specific conjugation to a species-matched or -mismatched albumin. Biomater Sci. 6(8):2092-2100. doi: 10.1039/c8bm00456k.
    5. Obajemu, A.A. et al. (2017). IFN-λ4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling. J Immunol. 199(11):3808-3820. doi: 10.4049/jimmunol.1700807.
    6. Shrestha, R.P. et al (2017). Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica. Microb Cell Fact. 16(1):145. doi: 10.1186/s12934-017-0760-3.
    7. Fowler, K.A. et al. (2017). Targeting the Canonical Nuclear Factor-κB Pathway with a High-Potency IKK2 Inhibitor Improves Outcomes in a Mouse Model of Idiopathic Pneumonia Syndrome. Biol Blood Marrow Transplant. doi: 10.1016/j.bbmt.2017.01.083.
    8. Gordon, E. D. et al. (2016). Alternative splicing of interleukin-33 and type 2 inflammation in asthma. Proc Natl Acad Sci U S A. 113:8765-8770.
    9. Yang, B. et al. (2016). Site-specific albumination as an alternative to PEGylation for the enhanced serum half-life in vivo. Biomacromolecules. doi:10.1021/acs.biomac.6b00238.
    10. Vance, M. et al. (2016). AAV gene therapy for MPS1-associated corneal blindness. Sci Rep. doi:10.1038/srep22131.
    11. Johnson, K. A. et al. (2016). The Ebola Virus matrix protein, VP40, requires phosphatidylinositol 4, 5-bisphosphate (PI (4, 5) P2) for extensive oligomerization at the plasma membrane and viral egress.  Sci Rep. 6:19125.
    12. Chen, Z. et al. (2016). GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway. Cell Death Dis. doi:10.1038/cddis.2015.300.
    13. Chen, C. C. et al. (2015). Changes in DNA methylation are associated with the development of drug resistance in cervical cancer cells. Cancer Cell Int. 15:98.
    14. Borjan, B. et al. (2015). The Aplidin analogs PM01215 and PM02781 inhibit angiogenesis in vitro and in vivo. BMC Cancer. 15:738.
    15. Gee, H. Y. et al. (2015). KANK deficiency leads to podocyte dysfunction and nephrotic syndrome. J Clin Invest. doi: 10.1172/JCI79504. 
    16. Vemula, S. V. et al. (2015). HIV-1 induced Nuclear Factor IB (NF-IB) expression negatively regulates HIV-1 replication through interaction with the long terminal repeat region. Viruses.  7:543-558.
    17. Zhang, Y. et al. (2015). Characterization of the promoter of Grapevine vein clearing virus. J Gen Virol. 96:165-169.
    18. Ott, L. & Carson, S. (2014). Immunological tools: Engaging students in the use and analysis of flow cytometry and enzyme‐linked immunosorbent assay (ELISA). Biochem Mol Biol Educ.  42:382-397.
    19. Fulton, L. M. et al. (2014). Altered T-cell entry and egress in the absence of Coronin 1A attenuates murine acute graft versus host disease.Eur J Immunol. 44:1662-1671.
    20. Baez, A. et al. (2014).  Production of recombinant protein by a novel oxygen-induced system in Escherichia coli. Microb Cell Fact. 13:50.
    21. Huhtala, T. et al. (2014). Biodistribution and antitumor effect of Cetuximab-targeted lentivirus. Nucl Med Biol. 41:77-83.
    22. Anyaegbu, C.  C. et al. (2014). Chemotherapy enhances cross-presentation of nuclear tumor antigens. PLoS One.  9:e107894.
    23. Sendra, L. et al. (2014). Low RNA translation activit limits the efficacy of hydrodynamic gene transfer to pig liver in vivo. J Gene Med. 16:179-192.
    24. Mango, R. et al. (2014). C-C Chemokine Receptor 5 on Pulmonary Mesenchymal Cells Promotes Experimental Metastasis via the Induction of Erythroid Differentiation Regulator 1. Mol. Cancer. Res. 12:274-282.
    25. Mitchell, A. et al. (2014). Promyelocytic Leukemia Protein Is a Cell-Intrinsic Factor Inhibiting Parvovirus DNA Replication. J. Virol. 88:925-936.
    26. Coghill, J.M. et al. (2013). CC chemokine Receptor 8 Potentiates Donor Treg Survival and is Critical for the Prevention of Murine Graft-Versus-Host Disease. Blood. 122:825-836.
    27. Pedersen, J. et al. (2012). Glucose Metabolism is Altered After Loss of L Cells and α-cells but Not Influenced by Loss of K Cells. Am J Physiol Endocrinol Metab. 304:E60-E73.
    28. Zhou, W. et al. (2012).Inducible Podocyte Injury and Proteinuria in Transgenic Zebrafish. J. Am. Soc. Nephrol. 23:1039-1047.
    29. Fonseca, J.P. et al. (2012). In Vivo Polycomb Kinetics and Mitotic Chromatin Binding Distinguish Stem Cells from Differentiated Cells. Genes and Dev. 26:857-871.
    30. Rajan, S. et al. (2010). In Vitro Processing and Secretion of Mutant Insulin Proteins that Cause Permanent Neonatal Diabetes. Am. J. Physiol. Endocrinol. Metab. 298:E403-E410.
AKR-120 　
    1. Occhialini, A. et al. (2020). Generation, analysis, and transformation of macro-chloroplast Potato (Solanum tuberosum) lines for chloroplast biotechnology. Sci Rep. 10(1):21144. doi: 10.1038/s41598-020-78237-x.
    2. Ahn, G. et al. (2019). Controlling the Release Profile Through Phase Control of Calcium Phosphate-Alginate Core-shell Nanoparticles in Gene Delivery. Macromolecular Research. doi: 10.1007/s13233-019-7106-4.
    3. Liu, J. et al. (2019). Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry. Sci Rep. 8(1):13442. doi: 10.1038/s41598-018-31713-x.
    4. Nichols, N. N. et al. (2018). Use of green fluorescent protein to monitor fungal growth in biomass hydrolysate. Biology Methods and Protocols. 3(1). doi: 10.1093/biomethods/bpx012.
    5. Lipták, N. et al. (2018). Glomerulosclerosis in transgenic rabbits with ubiquitous Venus protein expression. Acta Vet Hung. 66(2):281-293. doi: 10.1556/004.2018.026.
    6. Shim, M. S. et al. (2014). Stimuli-responsive siRNA carriers for efficient gene silencing in tumors via systemic delivery. Biomater Sci. 2:35-40.
    7. Pfeiffer, B. et al. (2012). Using Translational Enhancers to Increase Transgene Expression in Drosophila. PNAS. 109:6626-6631.(#AKR-120)
    8. Wamboldt, Y. et al. (2009). Participation of Leaky Ribosome Scanning in Protein Dual Targeting by Alternative Translation Initiation in Higher Plants. Plant Cell 10.1105/tpc.108.063644. (#AKR-120)
AKR-122

Fang, J. et al. (2015). COPII dependent ER export: a critical component of insulin biogenesis and beta cell ER homeostasis. Mol Endocrinol. doi:10.1210/me.2015-1012.

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
GFP ELISA Kit AKR-121 96 assays CELL BIOLABS
GFP ELISA Kit ARG82206 96 wells ARIGO
GFP ELISA Kit AKR-121-5 5x96 assays CELL BIOLABS
GFP Quantitation Kit AKR-120 100 assays CELL BIOLABS
Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative 800-420-GFP 1 kit ALPHA DIAGNOSTICS
Recombinant EGFP STA-201 100 ug CELL BIOLABS
Recombinant EGFP STA-201-5 5x100 ug CELL BIOLABS
RFP ELISA Kit AKR-122 96 assays CELL BIOLABS
β-Galactosidase Staining Kit AKR-100 1 kit CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
AKR-121
Product
GFP ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82206
Product
GFP ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
AKR-121-5
Product
GFP ELISA Kit
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
AKR-120
Product
GFP Quantitation Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ALPHA DIAGNOSTICS
Cat.No.
800-420-GFP
Product
Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative
Size
1 kit
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-201
Product
Recombinant EGFP
Size
100 ug
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-201-5
Product
Recombinant EGFP
Size
5x100 ug
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
AKR-122
Product
RFP ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
AKR-100
Product
β-Galactosidase Staining Kit
Size
1 kit
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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