Cas9 ELISA Kit | Cas9 단백질 정량 | 고마바이오텍

특징 

• 세포 또는 조직에서 만든 lysate에서 S. pyogenes Cas9의 검출과 정량을 위해 개발된 면역분석 ELISA Kit 입니다. 
• Sensitivity limit : 1.5 ng/mL of S. pyogenes Cas9
  

구성

• Anti-Cas9 Antibody Coated Plate
• Biotinylated Anti-Cas9 Antibody (1000X) 
• Streptavidin-Enzyme Conjugate 
• Assay Diluent 
• 10X Wash Buffer
• Substrate Solution
• Stop Solution 
• Cas9 Standard
  

데이터

Cas9 ELISA Standard Curve

Detection of Cas9 in Transfected 293 cells. Cells were transiently transfected with a Cas9

mammalian expression vector or mock transfected. After 48 hours, cells were lysed in RIPA buffer

and protein concentration was determined. The Cas9 ELISA kit was performed in the absence of cell

lysate (Neg), the presence of 50 ng/mL Cas9 Nuclease (Pos) (NEB catalog number M0386), the

presence of 10 µg protein lysate of mock transfected (Mock), or Cas9 transfected (Cas9).

사용 논문

PRB-5079

Van Cleemput, J. et al. (2021). CRISPR/Cas9-Constructed Pseudorabies Virus Mutants Reveal the Importance of UL13 in Alphaherpesvirus Escape from Genome Silencing. J Virol. 95(6):e02286-20. doi: 10.1128/JVI.02286-20. Lee, M.H. et al. (2021).  Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles. Mater Today Bio. 9:100091. doi: 10.1016/j.mtbio.2020.100091.

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관련제품

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.

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자료

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