Cas9 단백질 정량·활성 분석 키트 | Cas9 ELISA & Activity Assay Kit

특징

세포 또는 조직에서 만든 lysate에서 S. aureus와 S. pyogenes의 Cas9 단백질을 정량하는 ELISA kit와 활성을 측정하는 activity assay kit를 제공합니다.

Sensitivity limit

ELISA kit: 1.5 ng/mL of S. pyogenes Cas9, 3.9 ng/mL of S. aureus Cas9

Activity assay kit: 10 µg/mL of S. pyogenes Cas9, 4 µg/mL of S. aureus Cas9

구성

ELISA Kit

Anti-Cas9 Antibody Coated Plate
96-well Protein Binding Plate (S. aureus only)
100X Anti-SaCas9 Coating Antibody (S. aureus only)
Biotinylated Anti-Cas9 Antibody (1000X)
Streptavidin-Enzyme Conjugate
Assay Diluent
10X Wash Buffer
Substrate Solution
Stop Solution
Cas9 Standard

Activity Assay Kit

10X Assay Buffer
sgRNA
Target DNA
Cas9 Enzyme
Proteinase K

데이터

ELISA Kit

Cas9 ELISA Standard Curve

Detection of Cas9 in Transfected 293 cells. Cells were transiently transfected with a Cas9 mammalian expression vector or mock transfected. After 48 hours, cells were lysed in RIPA buffer and protein concentration was determined. The Cas9 ELISA kit was performed in the absence of cell lysate (Neg), the presence of 50 ng/mL Cas9 Nuclease (Pos) (NEB catalog number M0386), the presence of 10 µg protein lysate of mock transfected (Mock), or Cas9 transfected (Cas9).

Activity Assay Kit

Target DNA only (Lane 1) was incubated with S. pyogenes Cas 9 (SpCas9) only (Lane 2), single guide RNA (sgRNA) only (Lane 3), or SpCas9 and sgRNA (Lane 4).

293T cells were transfected with a plasmid expressing SaCas9 or an empty vector plasmid. After 24-48 hours, cells were lysed in lysis buffer. Target DNA (lane 1) in the presence of sgRNA was incubated with recombinant purified SaCas9 (lane 2), 85 µg of transfected 293T lysate containing SaCas9 (lane 3) or 85 µg of transfected 293T lysate containing an empty vector (lane 4). After incubation in Proteinase K, DNA fragments were cleaned and concentrated in a DNA Spin column prior to loading onto an 1.5% agarose gel.

사용 논문

PRB-5079

Geilenkeuser, J. et al. (2025). Engineered nucleocytosolic vehicles for loading of programmable editors. Cell. doi: 10.1016/j.cell.2025.03.015.
Fieni, C. et al. (2024). Immunoliposome-based targeted delivery of the CRISPR/Cas9gRNA-IL30 complex inhibits prostate cancer and prolongs survival. Exp Mol Med. doi: 10.1038/s12276-024-01310-2.
Mills, C. et al. (2022). A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs. CRISPR J. doi: 10.1089/crispr.2022.0056.
Van Cleemput, J. et al. (2021). CRISPR/Cas9-Constructed Pseudorabies Virus Mutants Reveal the Importance of UL13 in Alphaherpesvirus Escape from Genome Silencing. J Virol. 95(6):e02286-20. doi: 10.1128/JVI.02286-20.
Lee, M.H. et al. (2021). Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles. Mater Today Bio. 9:100091. doi: 10.1016/j.mtbio.2020.100091.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
Cas9 (CRISPR Associated Protein 9) Activity Assay Kit (S. aureus)	PRB-5203	20 assays	CELLBIOLABS
Cas9 (CRISPR Associated Protein 9) Activity Assay Kit (S. pyogenes)	PRB-5206	20 assays	CELLBIOLABS
Cas9 (CRISPR Associated Protein 9) ELISA Kit	PRB-5079	96 assays	CELL BIOLABS
Cas9 (CRISPR Associated Protein 9) ELISA Kit (S. aureus)	PRB-5204	96 assays	CELLBIOLABS

1
Maker	CELLBIOLABS
Cat.No.	PRB-5203
Product	Cas9 (CRISPR Associated Protein 9) Activity Assay Kit (S. aureus)
Size	20 assays
Qty
Data Sheet
MSDS

2
Maker	CELLBIOLABS
Cat.No.	PRB-5206
Product	Cas9 (CRISPR Associated Protein 9) Activity Assay Kit (S. pyogenes)
Size	20 assays
Qty
Data Sheet
MSDS