DNA 전기영동 시간 단축을 위한 Go-Go™ Fast DNA Gel Running Buffer | 고마바이오텍

특징

• TAE나 TBE를 사용할 때보다 높은 voltage로 런닝하여 전기영동 시간을 최대 3배 단축할 수 있습니다. (예, 1X TBE 이용 시 100V에서 60-90분이라면, Go-Go Fast gel running buffer 이용 시 250-300V에서 30분으로 시간을 단축할 수 있습니다.)
• 해상도가 높은 선명한 밴드를 제공합니다. 
• GelRed®, GelGreen® 등의 많이 사용되는 gel stain과 함께 사용할 수 있습니다.
• 50배 농축 용액으로 제공되어 1x로 희석하여 사용합니다.

실험 결과

그림 1. Go-Go Fast™ DNA Running Buffer allows gels to be run up to 3X faster than TBE or TAE, and shows superior DNA band resolution compared to traditional gel running buffers. DNA samples were separated on 11 cm long 1% agarose gels precast with 1X GelRed® DNA Gel Stain. Each gel was cast and run using the buffer indicated. Lanes 1-4: Biotium’s Ready-to-Use 100 bp DNA Ladder from 200 ng to 25 ng per lane. Lanes 6-9: Biotium’s Ready-to-Use 1 kb DNA Ladder from 200 ng to 25 ng per lane. Lane 11: 100 ng pUC19 plasmid (NEB) linearized with XbaI (2686 bp). Lane 12: 100 ng undigested pUC19 plasmid showing fast-migrating supercoiled plasmid. Top left: 1X Go-Go Fast™ Buffer run at 300 V (27 V/cm) for 30 minutes; top right: 1X TBE buffer run at 100 V (9 V/cm) for 90 minutes; bottom left: 1X TAE buffer run at 100 V (9 V/cm)for 90 minutes; bottom right: lithium borate (LiB) buffer run at 300 V (27 V/cm) for 30 minutes.

그림 2. GelRed® Prestain Plus 6X DNA Loading Dye was used to load 1 kb DNA ladder 1% agarose gels cast in 1X Go-Go™ Fast DNA Gel Buffer (left) or 1X TBE (right). The Go-Go™ Fast gel was run at 300 V (27 V/cm) for 30 minutes, while the TBE gel was run at 100 V (9 V/cm) for 90 minutes. Lanes 1-4: 1 kb DNA Ladder from 200 ng to 25 ng per lane.