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제품 상세
Nitration | Protein Oxidation | Nitrotyrosine ELISA Kit 이미지

Nitration | Protein Oxidation | Nitrotyrosine ELISA Kit

ROS (Reactive Oxygen Species 활성산소)와 reactive nitrogen species (RNS)에 의해 손상을 입은 단백질 AOPP, BPDE Protein Adduct, Nitrotyrosine, Protein Carbonyl을 확인하는 kit 입니다.

프린트 제품 문의
OxiSelect™ AOPP Assay Kit (#STA-318)

  • Cell lysates, tissue homogenates, plasma등의 샘플에서 AOPP (Advanced Oxidation Protein Products)를 측정합니다.
  • 30분안에 신속히 결과를 얻을수 있습니다.
  • 일반 microplate reader를 이용하므로 편리합니다.



OxiSelect™ BPDE Protein Adduct ELISA Kit (#STA-301)

  • Benzo(a)pyrene은 Cytochrome P450 (CYP)와 epoxide hydrolase (EH)에 의해 다양한 metabolite를 촉매하고 그 결과 final carcinogen BPDE를 생성합니다. 
  • BPDE-protein adducts를 검출하기 위한 ELISA Kit 입니다. 




OxiSelect™ Nitrotyrosine ELISA Kit (#STA-305)

  • ELISA 방법으로 3-Nitrotyrosine 형성을 측정합니다.
  • plasma, serum, cell lysates, purified proteins등의 샘플을 이용할 수 있습니다.
  • Nitrated BSA가 positive control로 제공됩니다.


OxiSelect™ Protein Carbonyl Assays 

  

  • protein carbonyl 형성을 Western blot 및 ELISA, 혹은 형광법으로 측정합니다.
  • plasma, serum, cell lysates, purified proteins등의 샘플을 이용할 수 있습니다.
  • Immunoblot format을 이용시에는, membrane상에서 DNPH derivatization을 통해 oxidized & non-oxidized protein을 바로 비교할 수 있습니다.
  • ELISA format을 이용시에는 10 ug/ml까지도 민감하게 detection 할 수 있습니다.
  • 농축이나 침전등의 과정을 거치지 않아 샘플 손실우려가 적습니다.
  • 종류:
    · STA-310 : OxiSelect™ Protein Carbonyl ELISA Kit
    · STA-307 : OxiSelect™ Protein Carbonyl Fluorometric Assay
    · STA-308 : OxiSelect™ Protein Carbonyl Immunoblot Kit

 

 


사용 논문

STA-305 　
    1. Całyniuk, Z. et al. (2021). Selected metabolic, epigenetic, nitration and redox parameters in turkeys fed diets with different levels of arginine and methionine. Ann. Anim. Sci. doi: 10.2478/aoas-2021-0069.
    2. Ognik, K. et al. (2021). The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine. Sci Rep. 11(1):15975. doi: 10.1038/s41598-021-95529-y.
    3. Williamson-Reisdorph, C.M. et al. (2021). Blood oxidative stress and post-exercise recovery are unaffected byhypobaric and hypoxic environments. J Sports Sci. doi: 10.1080/02640414.2021.1872960.
    4. Baker, B.C. et al. (2021). Hypoxia and oxidative stress induce sterile placental inflammation in vitro. Sci Rep. 11(1):7281. doi: 10.1038/s41598-021-86268-1.
    5. Šutulović, N. et al. (2021). Experimental Chronic Prostatitis/Chronic Pelvic Pain Syndrome Increases Anxiety-Like Behavior: The Role of Brain Oxidative Stress, Serum Corticosterone, and Hippocampal Parvalbumin-Positive Interneurons. Oxid Med Cell Longev. doi: 10.1155/2021/6687493.
    6. Jankowski, J. et al. (2021). The effect of different dietary ratios of lysine, arginine and methionine on protein nitration and oxidation reactions in turkey tissues and DNA. Animal. doi: 10.1016/j.animal.2021.100183.
    7. Dietrich-Muszalska, A. et al. (2021). Comparative Study of the Effects of Atypical Antipsychotic Drugs on Plasma and Urine Biomarkers of Oxidative Stress in Schizophrenic Patients. Neuropsychiatr Dis Treat. 17:555-565. doi: 10.2147/NDT.S283395.
    8. Zhou, Q. et al. (2021). Physiological Responses of Microcystis aeruginosa to Extracellular Degradative Enzymes and Algicidal Substance from Heterotrophic Bacteria. Pol. J. Environ. Stud. 30(3): 1-9. doi: 10.15244/pjoes/127867.
    9. Ohira, H. et al. (2021). Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer. PLoS One. 16(2):e0246580. doi: 10.1371/journal.pone.0246580.
    10. Uzunköprü, C. et al. (2021). Retinal Nerve Fiber Layer Thickness Correlates with Serum and Cerebrospinal Fluid Neurofilament Levels and is Associated with Current Disability in Multiple Sclerosis. Arch Neuropsychitry. doi: 10.29399/npa.27355.
    11. Gerszi, D. et al. (2021). Evaluation of oxidative/nitrative stress and uterine artery pulsatility index in early pregnancy. Physiol Int. doi: 10.1556/2060.2020.00041.
    12. Wong, T.H.T. et al. (2020). Consuming decaffeinated coffee with milk and sugar added before a high-glycaemic index meal improves postprandial glycaemic and insulinaemic responses in healthy adults. Br J Nutr. 124(8):785-796. doi: 10.1017/S0007114520001750.
    13. Gil, A. et al. (2020). Neuronal Metabolism and Neuroprotection: Neuroprotective Effect of Fingolimod on Menadione-Induced Mitochondrial Damage. Cells. 10(1):E34. doi: 10.3390/cells10010034.
    14. Miller, V.J. et al. (2020). A Ketogenic Diet Combined With Exercise Alters Mitochondrial Function In Human Skeletal Muscle While Improving Metabolic Health. Am J Physiol Endocrinol Metab. doi: 10.1152/ajpendo.00305.2020.
    15. Sprick, J.D. et al. (2020). Augmented exercise pressor response during maximal treadmill exercise is not related to systemic inflammation in stroke survivors. Top Stroke Rehabil. doi: 10.1080/10749357.2020.1806436.
    16. Hamidi, V. et al. (2020). Acute Exenatide Therapy Attenuates Postprandial Vasodilation in Humans with Prediabetes: A Randomized Controlled Trial. Metab Syndr Relat Disord. doi: 10.1089/met.2019.0102.
    17. Valvassori, S.S. et al. (2020). Protein kinase C isoforms as a target for manic-like behaviors and oxidative stress in a dopaminergic animal model of mania. Prog Neuropsychopharmacol Biol Psychiatry. doi: 10.1016/j.pnpbp.2020.109940.
    18. Vujacic-Mirski, K. et al. (2020). Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods. Antioxidants (Basel). 9(5). pii: E388. doi: 10.3390/antiox9050388.
    19. Chira, A. et al. (2020). Monocyte chemotactic protein‑1 and nitrotyrosine in irritable bowel syndrome. Exp Ther Med. doi: 10.3892/etm.2020.8665.
    20. Casós, K. et al. (2020). Determination of Redox Status in Serum. Methods Mol Biol. 2110:115-128. doi: 10.1007/978-1-0716-0255-3_8.
    21. Ruottinen, M. et al. (2020). The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer. Anticancer Res. 40(1):253-259. doi: 10.21873/anticanres.13947.
    22. Menegas, S. et al. (2020). Efficacy of folic acid as an adjunct to lithium therapy on manic-like behaviors, oxidative stress and inflammatory parameters in an animal model of mania. Metab Brain Dis. doi: 10.1007/s11011-019-00503-3.
    23. Mikolka, P. et al. (2019). Effect of different dosages of dexamethasone therapy on lung function and inflammation in an early phase of acute respiratory distress syndrome model. Physiol Res. 68(Supplementum 3):S253-S263. doi: 10.33549/physiolres.934364.
    24. Balasubramanian, P. et al. (2019). Obesity-Induced Sympathoexcitation is Associated with Nrf2 Dysfunction in the Rostral VentroLateral Medulla. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00206.2019.
    25. Streese, L. et al. (2019). Physical activity may drive healthy microvascular ageing via downregulation of p66Shc. Eur J Prev Cardiol. doi: 10.1177/2047487319880367.
    26. Chazarin, B. et al. (2019). Limited Oxidative Stress Favors Resistance to Skeletal Muscle Atrophy in Hibernating Brown Bears (Ursus Arctos). Antioxidants (Basel). 8(9). pii: E334. doi: 10.3390/antiox8090334.
    27. Suvakov, S. et al. (2019). Markers of Oxidative Stress and Endothelial Dysfunction Predict Haemodialysis Patients Survival. Am J Nephrol. doi: 10.1159/000501300.
    28. Jerotic, D. et al. (2019). Association of Nrf2, SOD2 and GPX1 Polymorphisms with Biomarkers of Oxidative Distress and Survival in End-Stage Renal Disease Patients. Toxins (Basel). 11(7). pii: E431. doi: 10.3390/toxins11070431.
    29. Simenauer, A. et al. (2019). Repression of Nrf2/ARE regulated antioxidant genes and dysregulation of the cellular redox environment by the HIV Transactivator of Transcription. Free Radic Biol Med. 141:244-252. doi: 10.1016/j.freeradbiomed.2019.06.015.
    30. Tucker, M.A. et al. (2019). Endothelial Dysfunction in Cystic Fibrosis: Role of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019:1-8. doi: 10.1155/2019/1629638.
STA-318 　
    1. Alses, M. & Alzeer, S. (2021). Evaluation of some biological parameters of gasoline station attendants in Damascus, Syria. Heliyon. 7(5):e07056. doi: 10.1016/j.heliyon.2021.e07056 (#STA-318).
    2. Nukala, S.B. et al. (2021). Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension. Sci Rep. 11(1):5583. doi: 10.1038/s41598-021-85004-z (#STA-318).
    3. AlMarabeh, S. et al. (2020). Chronic intermittent hypoxia impairs diuretic and natriuretic responses to volume expansion in rats with preserved low-pressure baroreflex control of the kidney. Am J Physiol Renal Physiol. doi: 10.1152/ajprenal.00377.2020 (#STA-318).
    4. Xiao, L.L. et al. (2020). Using advanced oxidation protein products and ischaemia-modified albumin to monitor oxidative stress levels in patients with drug-induced liver injury. Sci Rep. 10(1):18128. doi: 10.1038/s41598-020-75141-2 (STA-318).
    5. Bloomer, R.J. et al. (2020). Meta- bolic Health Outcomes Following Nine Months of Mild Caloric Restriction in Male Rats Adhering To a Western or Vegan Diet. J Altern Complement Integr Med. 6:097. doi: 10.24966/ACIM-7562/100097 (#STA-318).
    6. Bigazzi, R. et al. (2019). Hypertension in High School Students: Genetic and Environmental Factors: The HYGEF Study. Hypertension. 75(1):71-78. doi: 10.1161/HYPERTENSIONAHA.119.13818 (#STA-318).
    7. McAllister, M.J. et al. (2019). Acute coffee ingestion with and without medium chain triglycerides decreases blood oxidative stress markers and increases ketone levels. Can J Physiol Pharmacol. doi: 10.1139/cjpp-2019-0458 (#STA-318).
    8. Ungurianu, A. et al. (2019). Preclinical and clinical results regarding the effects of a plant-based antidiabetic formulation versus well established antidiabetic molecules. Pharmacol Res. 150:104522. doi: 10.1016/j.phrs.2019.104522 (#STA-318).
    9. Smith, C. et al. (2019). Chronic testosterone deprivation sensitizes the middle-aged rat brain to damaging effects of testosterone replacement. Neuroendocrinology. doi: 10.1159/000504445 (#STA-318).
    10. Gryszczyńska, B. et al. (2019). Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment. BioMed Research International. 2019(7976043)1-9 pages. doi: 10.1155/2019/7976043 (#STA-318).
    11. Morsy, M.D. et al. (2019). Protective effect of combined melatonin and α-tocopherol administration in spinal cord ischemia-reperfusion injury in rat. Int. J. Morphol. 37(2):428-437. doi: 10.4067/S0717-95022019000200428 (#STA-318).
    12. Albatayneh, E.M. et al. (2019). Serum Oxidative-Antioxidative Status in Patients With Alkaptonuria. J Clin Med Res. 11(5):337-344. doi: 10.14740/jocmr3801 (#STA-318).
    13. Shell, B. et al. (2019). Angiotensin Type 1a Receptors in the Median Preoptic Nucleus Support Intermittent Hypoxia-Induced Hypertension. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00393.2018 (#STA-318).
    14. Bloomer, R. et al. (2018) Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women. Health. 10:960-970. doi: 10.4236/health.2018.107071 (#STA-318).
    15. Sighinolfi, G. et al. (2018). AB0760 Advanced oxidation protein products in serum of patients with systemic sclerosis: a possible indicator of clinical evolution. Annals of the Rheumatic Diseases. 77:1516. doi: 10.1136/annrheumdis-2018-eular.6809 (#STA-318).
    16. Wilson, E.N. et al. (2018). Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging. Physiol Behav. 189:64-73. doi: 10.1016/j.physbeh.2018.03.007 (#STA-318).
    17. Gradinaru, D. et al. (2018). Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. doi: 10.1055/s-0043-123825 (#STA-318).
    18. Hányšová, S. et al. (2017). Elevated plasma levels of advanced oxidation protein products in Slovak multiple sclerosis patients: possible association with different disability states. Act Nerv Super Rediviva. 59(2): 45–50 (#STA-318).
    19. Sun, S. et al. (2018). Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway. Redox Biol. 14:338-353. doi: 10.1016/j.redox.2017.09.011 (#STA-318).
    20. Snyder, B. et al. (2017). Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol. Rep. doi: 10.14814/phy2.13258 (#STA-318).
    21. Budzyń, M. et al. (2017). The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm. Hindawi BioMed Research International. doi: 10.1155/2017/2791082 (#STA-318).
    22. Wan, X. et al. (2016). SIRT1-PGC1a-NFkB pathway of oxidative and inflammatory stress during Trypanosoma cruzi infection: benefits of SIRT1-targeted therapy in improving heart function in Chagas disease. PLoS Pathog. doi: 10.1371/journal.ppat.1005954 (#STA-318).
    23. Gradinaru, D. et al. (2016). Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. doi:10.1007/s40520-016-0629-z (#STA-318).
    24. Crone, L. B. et al. (2016). Impact of meal ingestion rate and caffeine coingestion on postprandial lipemia and oxidative stress following high-fat meal consumption. J Caffeine Res. doi:10.1089/jcr.2016.0004 (#STA-318).
    25. Huemer, M. et al. (2016). Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency. J Inherit Metab Dis. doi:10.1007/s10545-016-9928-y (#STA-318).
    26. Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582 (#STA-318).
    27. Šarić, B. et al. (2015). Oxidative stress impact on growth hormone secretion in the eye. Croat Med J. 56:326-333 (#STA-318).
    28. Bloomer, R. J. et al. (2015). Comparison of a restricted and unrestricted vegan diet plan with a restricted omnivorous diet plan on health-specific measures. Healthcare.3:544-555 (#STA-318).
    29. Jung, E. et al. (2015). Gemigliptin improves renal function and attenuates podocyte injury in mice with diabetic nephropathy. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.04.055 (#STA-318).
    30. Thurmond, P. et al. (2015). Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia. Korean J Urol. 56:187-196 (#STA-318).

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
BPDE Protein Adduct ELISA Kit ARG81226 96 wells ARIGO
OxiSelect™ AOPP Assay Kit (200 assays) STA-318 200 assays CELL BIOLABS
OxiSelect™ BPDE Protein Adduct ELISA Kit STA-301 96 assays CELL BIOLABS
OxiSelect™ Nitrotyrosine ELISA Kit (1 plate) STA-305 96 assays CELL BIOLABS
OxiSelect™ Nitrotyrosine ELISA Kit (5 plates) STA-305-5 5x96 assays CELL BIOLABS
OxiSelect™ Protein Carbamylation Sandwich ELISA Kit STA-877 96 assays CELL BIOLABS
OxiSelect™ Protein Carbonyl ELISA Kit STA-310 96 assays CELL BIOLABS
OxiSelect™ Protein Carbonyl ELISA Kit (5 plates) STA-310-5 5x96 assays CELL BIOLABS
OxiSelect™ Protein Carbonyl Fluorometric Assay STA-307 100 assays CELL BIOLABS
OxiSelect™ Protein Carbonyl Immunoblot Kit STA-308 10 blots CELL BIOLABS
QuantiChrom™ Carbonyl Assay Kit DCAR-100 100 assays BIOASSAY SYSTEMS
전체보기
Maker
ARIGO
Cat.No.
ARG81226
Product
BPDE Protein Adduct ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-318
Product
OxiSelect™ AOPP Assay Kit (200 assays)
Size
200 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-301
Product
OxiSelect™ BPDE Protein Adduct ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-305
Product
OxiSelect™ Nitrotyrosine ELISA Kit (1 plate)
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-305-5
Product
OxiSelect™ Nitrotyrosine ELISA Kit (5 plates)
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-877
Product
OxiSelect™ Protein Carbamylation Sandwich ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-310
Product
OxiSelect™ Protein Carbonyl ELISA Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-310-5
Product
OxiSelect™ Protein Carbonyl ELISA Kit (5 plates)
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-307
Product
OxiSelect™ Protein Carbonyl Fluorometric Assay
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-308
Product
OxiSelect™ Protein Carbonyl Immunoblot Kit
Size
10 blots
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DCAR-100
Product
QuantiChrom™ Carbonyl Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
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