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Oxidase/Peroxidase Activity Assays 이미지

Oxidase/Peroxidase Activity Assays

Monoamine Oxidase, Myeloperoxidase Activity, Hydrogen Peroxide/Peroxidase등 Oxidase 및 Peroxidase의 활성을 측정하는 Assay kit을 소개합니다.

프린트 제품 문의

OxiSelect™ Monoamine Oxidase Assay Kit (#XPX-5006/XPX-5000)

  

  • 모노아민 산화효소 (Monoamine oxidases, MAO)는 모노아민의 산화적 탈 아미노화를 촉매하는 미토콘드리아 membrane에서 발견되는 효소로, MAO-A, MAO-B의 두가지 isoform으로 존재합니다.
  • MAO는 신경전달 물질을 조절하고, 기능적으로 장애가 있을 경우 우울증, 약물남용, 편두통, 정신분열증, 주의력 결핍장애, 파킨슨병, 알츠하이머 병과 관련이 있습니다.
  • MAO는 기질과 반응하여 H2O2를 생성하고 이것이 probe와 반응합니다. 즉 MAO가 많을수록 컬러값 및 형광값이 높게 측정됩니다. 
  • 종류 :
    - OxiSelect™ Monoamine Oxidase Assay Kit (Colorimetric) (#XPX-5006)
    - OxiSelect™ Monoamine Oxidase Assay Kit (Fluorometric) (#XPX-5000)

 

 

 

OxiSelect™ Myeloperoxidase Activity Assay Kit (#STA-803/STA-804)


  • Myeloperoxidase (MPO)는 많은 질병과 관련된 heme-기반의 과산화효소이며, MPO 수준의 상승은 관상동맥질환과 관련이 있습니다. 
  • 과산화수소가 존재하면 MPO는 활성 산화환원 중간형태 (MPO-I)로 전환되어 chloride ion을 hypochlorous acid로 전환시키는 염소화 반응과 native state로 전화되는 과산화반응을 유도합니다.  
  • 종류 :
    -     Myeloperoxidase Chlorination Activity Assay, Colorimetric (#STA-803)
    - Myeloperoxidase Chlorination Activity Assay, Fluorometric (#STA-804)

 

 

 

OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit (#STA-344/STA-844)

  

  • 과산화수소(Hydrogen Peroxide, H2O2) 및 과산화효소(Hydrogen Peroxidase, HRP)를 측정하기 위한 정량분석 킷트 입니다.
  • HRP가 존재하는 경우 H2O2와 반응하여 540-570nm에서 컬러반응으로 측정되거나 530-560/590nm에서 형광으로 측정됩니다. 
  • 미지시료내의 H2O2 및 HRP의 함량은 표준곡선과 비교하여 결정됩니다. 
  • cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine 및 기타 biological fluids에 적용됩니다. 
  • 검출범위 : 0.8 µM (colorimetric), 50 nM (fluorometric)
  • 종류 :
    - OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit (Colorimetric) (#STA-844)
    - OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric) (#STA-344)

 



사용 논문

XPX-5006
XPX-5000		 　
    1. Yirun, A. et al. (2021). Neuroendocrine disruption by bisphenol A and/or di(2-ethylhexyl) phthalate after prenatal, early postnatal and lactational exposure. Environ Sci Pollut Res Int. doi: 10.1007/s11356-021-12408-9.
    2. Akiyama, S. et al. (2021). β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes. Appl. Sci. 11(7):3034. doi: 10.3390/app11073034 (#XPX-5006).
    3. Hamouda, A.F. et al. (2019). A Biochemical Study of Agarwood on Methanol Injection in Rat. Journal of Drug and Alcohol Research. 8(2019):236073. doi: 10.4303/jdar/236073 (#XPX-5006).
    4. Ano, Y. et al. (2019). Tryptophan-Tyrosine Dipeptide, the Core Sequence of β-Lactolin, Improves Memory by Modulating the Dopamine System. Nutrients. 11(2). pii: E348. doi: 10.3390/nu11020348 (#XPX-5006).
    5. AL‑Shammaree, S.A.W. (2018). Semicarbazide-sensitive amine oxidase activity levels in patients with acute lymphoblastic leukemia after cytotoxic chemotherapy. Beni-Suef University Journal of Basic and Applied Sciences. 7 (2018) 683–68. doi: 10.1016/j.bjbas.2018.07.013 (#XPX-5006).
    6. Ano, Y. et al. (2018). Novel lactopeptides in fermented dairy products improve memory function and cognitive decline. Neurobiol Aging. 72:23-31. doi: 10.1016/j.neurobiolaging.2018.07.016 (#XPX-5006).
STA-344
STA-844		 　
    1. Balmant, K.M. et al. (2021). Guard cell redox proteomics reveals a role of lipid transfer protein in plant defense. J Proteomics. doi: 10.1016/j.jprot.2021.104247 (#STA-344).
    2. Odatsu, T. et al. (2021). Lactoferrin with Zn-ion protects and recovers fibroblast from H2O2-induced oxidative damage. Int J Biol Macromol. 190:368-374. doi: 10.1016/j.ijbiomac.2021.08.214 (#STA-844).
    3. Graham, R.J. et al. (2021). Zinc supplementation modulates intracellular metal uptake and oxidative stress defense mechanisms in CHO cell cultures. Biochem Eng J. doi: 10.1016/j.bej.2021.107928 (#STA-844).
    4. Gromotowicz-Poplawska, A. et al. (2021). Hyperglycemia Potentiates Prothrombotic Effect of Aldosterone in a Rat Arterial Thrombosis Model. Cells. 10(2):471. doi: 10.3390/cells10020471 (#STA-844).
    5. Chiang, C.C. et al. (2020). Aqueous Extract of Kan-Lu-Hsiao-Tu-Tan Ameliorates Collagen-Induced Arthritis in Mice by Inhibiting Oxidative Stress and Inflammatory Responses. Life (Basel). 10(12):E313. doi: 10.3390/life10120313 (#STA-844).
    6. Hwang, D.K. et al. (2020). Changes in the Systemic Expression of Sirtuin-1 and Oxidative Stress after Intravitreal Anti-Vascular Endothelial Growth Factor in Patients with Retinal Vein Occlusion. Biomolecules. 10(10):1414. doi: 10.3390/biom10101414 (#STA-344).
    7. Sabry, M.M. et al. (2020). Carnitine, apelin and resveratrol regulate mitochondrial quality control (QC) related proteins and ameliorate acute kidney injury: role of hydrogen peroxide. Arch Physiol Biochem. doi: 10.1080/13813455.2020.1773504 (#STA-844).
    8. Nitta, Y. et al. (2020). Catalase is required for peroxisome maintenance during adipogenesis. Biochim Biophys Acta Mol Cell Biol Lipids. doi: 10.1016/j.bbalip.2020.158726 (#STA-344).
    9. Ansar, M. et al. (2020). Increased Lung Catalase Activity Confers Protection Against Experimental RSV Infection. Sci Rep. 10(1):3653. doi: 10.1038/s41598-020-60443-2 (#STA-344).
    10. Yoshimoto, S. et al. (2020). Riboflavin Plays a Pivotal Role in the UVA-Induced Cytotoxicity of Fibroblasts as a Key Molecule in the Production of H2O2 by UVA Radiation in Collaboration with Amino Acids and Vitamins. Int J Mol Sci. 21(2). pii: E554. doi: 10.3390/ijms21020554 (#STA-344).
    11. Ścibior, A. et al. (2020). In vitro effect of vanadyl sulfate on cultured primary astrocytes: cell viability and oxidative stress markers. J Appl Toxicol. doi: 10.1002/jat.3939 (#STA-844).
    12. El-Boshy, M. et al. (2020). Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti-inflammatory actions by remodeling cellular calcium pathways. J Biochem Mol Toxicol. doi: 10.1002/jbt.22440 (#STA-844).
    13. Anand, S. et al. (2019). Agastache honey has superior antifungal activity in comparison with important commercial honeys. Sci Rep. 9(1):18197. doi: 10.1038/s41598-019-54679-w (#STA-344).
    14. Pei, J.F. et al. (2019). Diurnal oscillations of endogenous H2O2 sustained by p66Shc regulate circadian clocks. Nat Cell Biol. 21(12):1553-1564. doi: 10.1038/s41556-019-0420-4 (#STA-344).
    15. Mehta, N. et al. (2019). Follistatin Protects against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease. Antioxid Redox Signal. doi: 10.1089/ars.2018.7684 (#STA-344).
    16. Lee, D.H. et al. (2019). Peroxiredoxin 6 confers protection against non-alcoholic fatty liver disease through maintaining mitochondrial function. Antioxid Redox Signal. doi: 10.1089/ars.2018.7544 (#STA-344).
    17. Oncul, S. et al. (2019). A Kojic Acid Derivative Promotes Intrinsic Apoptotic Pathway of Hepatocellular Carcinoma Cells without Incurring Drug Resistance. Chem Biol Drug Des. doi: 10.1111/cbdd.13615 (#STA-844).
    18. Nishu, S.D. et al. (2019). Nutritional status regulates algicidal activity of Aeromonas sp. L23 against cyanobacteria and green algae. PLoS One. 14(3):e0213370. doi: 10.1371/journal.pone.0213370 (#STA-844).
    19. Sakai, T. et al. (2019). Cytoplasmic OH scavenger TA293 attenuates cellular senescence and fibrosis by activating macrophages through oxidized phospholipids/TLR4. Life Sci. 221:284-292. doi: 10.1016/j.lfs.2019.02.038 (#STA-844).
    20. Abdrabouh, A. E. (2019). Liver disorders related to exposure to gasoline fumes in male rats and role of fenugreek seed supplementation. Environ Sci Pollut Res Int. 26(9):8949-8957. doi: 10.1007/s11356-019-04307-x (#STA-844).
    21. Sakai, T. et al. (2019). Hydrogen Indirectly Suppresses Increases in Hydrogen Peroxide in Cytoplasmic Hydroxyl Radical-Induced Cells and Suppresses Cellular Senescence. Int J Mol Sci. 20(2). pii: E456. doi: 10.3390/ijms20020456 (#STA-844).
    22. Nguyen, K. H. et al. (2018). Overexpression of GmNAC085 enhances drought tolerance in Arabidopsis by regulating glutathione biosynthesis, redox balance and glutathione-dependent detoxification of reactive oxygen species and methylglyoxal. Environmental and Experimental Botany. doi:10.1016/j.envexpbot.2018.12.021 (#STA-344).
    23. Mu, H.N. et al. (2018). Caffeic acid attenuates rat liver injury after transplantation involving PDIA3-dependent regulation of NADPH oxidase. Free Radic Biol Med. 129:202-214. doi: 10.1016/j.freeradbiomed.2018.09.009 (#STA-344).
    24. Bucekova, M. et al (2018). Microwave processing of honey negatively affects honey antibacterial activity by inactivation of bee-derived glucose oxidase and defensin-1. Food Chemistry. 240: 1131-1136 (#STA-344).
    25. Gamal, M. et al. (2018). Possible involvement of tetrahydrobiopterin in the disturbance of redox homeostasis in sepsis - Induced brain dysfunction. Brain Res. 1685:19-28. doi: 10.1016/j.brainres.2018.02.008 (#STA-844).
    26. Badosa, E. (2017). Control of fire blight infections with synthetic peptides that elicit plant defense responses. Journal of Plant Pathology. 99 (Special issue), 65-73 (#STA-344).
    27. Ohta, Y. et al. (2017). Compound 48/80, a mast cell degranulator, causes oxidative damage by enhancing vitamin C synthesis via reduced glutathione depletion and lipid peroxidation through neutrophil infiltration in rat livers. J. Clin. Biochem. Nutr. doi: 10.3164/jcbn.16-89 (#STA-344).
    28. Endesfelder, S. et al. (2017). Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury. Int J Mol Sci. doi: 10.3390/ijms18010187 (#STA-344).
    29. Son, D.J. et al. (2016). Novel synthetic (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol inhibits arthritis by targeting signal transducer and activator of transcription 3. Sci. Rep. 6:36852 (#STA-344).
    30. Begieneman, M.P.V. et al. (2016). Dopamine induces lipid accumulation, NADPH oxidase-related oxidative stress, and a proinflammatory status of the plasma membrane in H9c2 cells. Am. J. Physiol. Heart Circ. Physiol. 311:H1097-H1107 (#STA-344).


 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
OxiSelect™ Monoamine Oxidase Assay Kit (Colorimetric) XPX-5006 96 assays CELL BIOLABS
OxiSelect™ Monoamine Oxidase Assay Kit (Colorimetric) XPX-5006-5 5x96 assays CELL BIOLABS
OxiSelect™ Monoamine Oxidase Assay Kit (Fluorometric) XPX-5000 96 assays CELL BIOLABS
OxiSelect™ Monoamine Oxidase Assay Kit (Fluorometric) XPX-5000-5 5x96 assays CELL BIOLABS
OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit (Colorimetric) STA-803 200 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
XPX-5006
Product
OxiSelect™ Monoamine Oxidase Assay Kit (Colorimetric)
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
XPX-5006-5
Product
OxiSelect™ Monoamine Oxidase Assay Kit (Colorimetric)
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
XPX-5000
Product
OxiSelect™ Monoamine Oxidase Assay Kit (Fluorometric)
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
XPX-5000-5
Product
OxiSelect™ Monoamine Oxidase Assay Kit (Fluorometric)
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-803
Product
OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit (Colorimetric)
Size
200 assays
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
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