고마바이오텍(주)

로그인이 필요합니다.

Category 전체보기
전기영동 > 단백질 전기영동 전기영동 > 헥산 전기영동
Cat. No.전체보기

검색 결과가 없습니다.

Product전체보기

검색 결과가 없습니다.

Brand전체보기

검색 결과가 없습니다.

제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

8-OHG RNA Damage ELISA (#STA-325) 

 

  • 산화에 의한 RNA 손상의 ubiquitous marker인 8-OHG를 ELISA 방법으로 정량합니다.
  • 검출범위 : 300 pg/mL - 40 ng/mL of 8-OHG
  • Urine, serum, cerebrospinal fluid, cell, tissue등의 샘플에 적용됩니다. 

 

 

 

8-OHdG DNA Damage ELISA (#STA-320)

 

  • 산화에 의한 DNA 손상의 ubiquitous marker인 8-OHdG를 ELISA 방법으로 정량합니다.
  • 검출범위 : 100 pg/mL of 8-OHG
  • Urine, serum, cell, tissue등의 샘플에 적용됩니다. 

 

 

 

AP Sites Quantitation Kit (#STA-324)

  

  • 손상 후 복구되지 않은 DNA base AP (apurinic/apyrimidinic) sites를 측정합니다.
  • Aldehyde Reactive Probe (ARP)를 이용하여 AP site의 open ring에 있는 aldehyde group과 반응시키고, Biotin, Streptavidin-Enzyme 과정으로 가시화 합니다. 
  • 검출 범위 : 4-40 AP sites in 10^5 bp DNA 

 

 

 

DNA Double-Strand Break Assay (#STA-321)

  

  • DNA의 double-strand break (DSB)는 DNA damage중 가장 위험한 타입의 손상으로, 세포는 histone varient H2AX를 인산화시키고 serine 잔기의 인산화는 chromatin 응축을 유발합니다.
  • phosphorylated histone H2AX의 면역형광 염색을 통해 세포내 존재하는 DSB를 측정합니다.

 

 
 

DNA Double-Strand Break Formation in A549 Cells. A549 cells were seeded at 50,000 cells/well overnight. 

(A) Untreated cells. (B) Cells treated with 100 µM Etoposide for 1 hour. Immunofluorescence staining was then performed according to the Assay Protocol. 

 

 

 

 

사용 논문

 

STA-325 　
    1. Li, H. et al. (2021). Striatal oxidative damages and neuroinflammation correlate with progression and survival of Lewy body and Alzheimer diseases. Neural Regen Res. 17(4):867-874. doi: 10.4103/1673-5374.322463.
    2. Pappas-Gogos, G. et al. (2021). Urine 8-Hydroxyguanine (8-OHG) in Patients Undergoing Surgery for Colorectal Cancer. J Invest Surg. doi: 10.1080/08941939.2021.1904466.
    3. Li, H. et al. (2019). The Interactions of Dopamine and Oxidative Damage in the Striatum of Neurodegenerative Diseases Patients. J Neurochem. doi: 10.1111/jnc.14898.
    4. Gmitterová, K. et al. (2018). DNA versus RNA oxidation in Parkinson's disease: Which is more important?. Neurosci Lett. 662:22-28. doi: 10.1016/j.neulet.2017.09.048.
    5. Siegfried,C.J. et al (2017).Effects of Vitrectomy and Lensectomy on Older Rhesus Macaques: Oxygen Distribution, Antioxidant Status, and Aqueous Humor Dynamics. Invest Ophthalmol Vis Sci. 58(10):4003-4014. doi: 10.1167/iovs.17-21890.
    6. Sliwinska, A. et al. (2016). The levels of 7, 8-dihydrodeoxyguanosine (8-oxoG) and 8-oxoguanine DNA glycosylase 1 (OGG1)–A potential diagnostic biomarkers of Alzheimer's disease. J Neurol Sci. 368:155-159.
    7. Belenky, P. et al. (2015).  Bactericidal antibiotics induce toxic metabolic perturbations that lead to cellular damage. Cell Rep. 13:968-980.
    8. Tsai, C. H. et al. (2015). Transcriptional Analysis of Deinococcus radiodurans Reveals Novel Small RNAs That Are Differentially Expressed under Ionizing Radiation. Appl Environ Microbiol. 81:1754-1764.
    9. Giannakopoulos, B. et al. (2014). Deletion of the antiphospholipid syndrome autoantigen β2‐glycoprotein I potentiates the lupus autoimmune phenotype in a Toll‐like receptor 7–mediated murine model. Arthritis Rheumatol. 66:2270-2280.
    10. Bazin, J. et al. (2011). Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry after-Ripening. Plant Cell 23:2196-2208. 
STA-320 　
    1. Całyniuk, Z. et al. (2021). Selected metabolic, epigenetic, nitration and redox parameters in turkeys fed diets with different levels of arginine and methionine. Ann. Anim. Sci. doi: 10.2478/aoas-2021-0069.
    2. Chan, T.K. et al. (2021). Polycyclic aromatic hydrocarbons regulate the pigmentation pathway and iinduce DNA damage responses in keratinocytes, a process driven by systemic immunity. J Dermatol Sci. doi: 10.1016/j.jdermsci.2021.09.003.
    3. Kim, H.C. et al. (2021). Glycyrrhizin ameliorating sterile inflammation induced by low-dose radiation exposure. Sci Rep. 11(1):18356. doi: 10.1038/s41598-021-97800-8.
    4. Li, H. et al. (2021). Striatal oxidative damages and neuroinflammation correlate with progression and survival of Lewy body and Alzheimer diseases. Neural Regen Res. 17(4):867-874. doi: 10.4103/1673-5374.322463.
    5. Pérez-Soto, E. et al. (2021). Proinflammatory and Oxidative Stress States Induced by Human Papillomavirus and Chlamydia trachomatis Coinfection Affect Sperm Quality in Asymptomatic Infertile Men. Medicina (Kaunas). 57(9):862. doi: 10.3390/medicina57090862.
    6. Boudjema, J. et al. (2021). Metal enriched quasi-ultrafine particles from stainless steel gas metal arc welding induced genetic and epigenetic alterations in BEAS-2B cells. NanoImpact. doi: 10.1016/j.impact.2021.100346.
    7. Li, J. & Min, Y. (2021). Pre-clinical evidence that salinomycin is active against retinoblastoma via inducing mitochondrial dysfunction, oxidative damage and AMPK activation. J Bioenerg Biomembr. 53(5):513-523. doi: 10.1007/s10863-021-09915-2.
    8. Ognik, K. et al. (2021). The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine. Sci Rep. 11(1):15975. doi: 10.1038/s41598-021-95529-y.
    9. Wadikar, D.L. et al. (2021). Assessment of occupational exposure to diesel particulate matter through evaluation of 1-nitropyrene and 1-aminopyrene in surface coal miners, India. Environ Monit Assess. 193(6):342. doi: 10.1007/s10661-021-09121-y.
    10. Fatima, S. et al. (2021). Epigallocatechin gallate and coenzyme Q10 attenuate cisplatin-induced hepatotoxicity in rats via targeting mitochondrial stress and apoptosis. J Biochem Mol Toxicol. doi: 10.1002/jbt.22701.
    11. Ahmad, A. et al. (2021). Swertia chirayita suppresses the growth of non-small cell lung cancer A549 cells and concomitantly induces apoptosis via downregulation of JAK1/STAT3 pathway. Saudi J Biol Sci. doi: 10.1016/j.sjbs.2021.06.085.
    12. Xue, Z. et al. (2021). Isorhapontigenin ameliorates cerebral ischemia/reperfusion injury via modulating Kinase Cε/Nrf2/HO-1 signaling pathway. Brain Behav. doi: 10.1002/brb3.2143.
    13. Jiang, J. et al. (2021). Impact of intrauterine fetal resuscitation with oxygen on oxidative stress in the developing rat brain. Sci Rep. 11(1):9798. doi: 10.1038/s41598-021-89299-w.
    14. Jacobson, M.H. et al. (2021). Organophosphate pesticides and progression of chronic kidney disease among children: A prospective cohort study. Environ Int. 155:106597. doi: 10.1016/j.envint.2021.106597.
    15. Oladosu, W.O. et al. (2021). Evaluating the Effects of Life styles and History of Exposure to Radiation on Levels of Significance and Severity of Sperm DNA Damage among Males with Infertility Using 8-Hydroxydeoxyguanosine (8-OHDG) as a Marker. EC Endocrinology and Metabolic Research. 6(4): 15-27.
    16. Shaw, P. et al. (2021). Cold Atmospheric Plasma Increases Temozolomide Sensitivity of Three-Dimensional Glioblastoma Spheroids via Oxidative Stress-Mediated DNA Damage. Cancers. 13(8):1780. doi: 10.3390/cancers13081780.
    17. Sener, T.E. et al. (2020). Effects of resveratrol against scattered radiation-induced testicular damage in rats. Turk Biyokim Derg. doi: 10.1515/tjb-2020-0320.
    18. Corinaldesi, C. et al. (2021). Multiple impacts of microplastics can threaten marine habitat-forming species. Commun Biol. 4(1):431. doi: 10.1038/s42003-021-01961-1.
    19. Wahjuni, S. et al. (2021). Green Mustard Ethanol Extract (Brassica Rapa L.) Leaf Can Cell Damage (8-Hydroxy-2-Dioxiguanosine) In The Wistar Rat Hyperglicemic. IOP Conf. Ser.: Earth Environ. Sci. doi: 10.1088/1755-1315/709/1/012046.
    20. Jankowski, J. et al. (2021). The effect of different dietary ratios of lysine, arginine and methionine on protein nitration and oxidation reactions in turkey tissues and DNA. Animal. doi: 10.1016/j.animal.2021.100183.
    21. Xie, W. et al. (2021). Pterostilbene accelerates wound healing by modulating diabetes-induced estrogen receptor β suppression in hematopoietic stem cells. Burns Trauma. doi: 10.1093/burnst/tkaa045.
    22. Guo, L. et al. (2021). Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor. Drug Des Devel Ther. 15:589-600. doi: 10.2147/DDDT.S294009.
    23. Lu, Y. et al. (2021). ShengMai-San Attenuates Cardiac Remodeling in Diabetic Rats by Inhibiting NOX-Mediated Oxidative Stress. Diabetes Metab Syndr Obes. doi: 10.2147/DMSO.S287582.
    24. Ohira, H. et al. (2021). Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer. PLoS One. 16(2):e0246580. doi: 10.1371/journal.pone.0246580.
    25. Cortés, S. et al. (2021). A Positive Relationship between Exposure to Heavy Metals and Development of Chronic Diseases: A Case Study from Chile. Int J Environ Res Public Health. 18(4):1419. doi: 10.3390/ijerph18041419.
    26. Yang, S.B. et al. (2021). A Hepatitis B Virus-Derived Peptide Exerts an Anticancer Effect via TNF/iNOS-producing Dendritic Cells in Tumor-Bearing Mouse Model. Cancers (Basel). 13(3):407. doi: 10.3390/cancers13030407.
    27. Liu, J. et al. (2021). NUPR1 is a critical repressor of ferroptosis. Nat Commun. 12(1):647. doi: 10.1038/s41467-021-20904-2.
    28. Wang, M. et al. Fluvastatin protects neuronal cells from hydrogen peroxide-induced toxicity with decreasing oxidative damage and increasing PI3K/Akt/mTOR signaling. J Pharm Pharmacol. doi: 10.1093/jpp/rgaa058.
    29. Tascanov, M.B. et al. (2021). Relationships between paroxysmal atrial fibrillation, total oxidant status, and DNA damage. Rev Port Cardiol. 40(1):5-10. doi: 10.1016/j.repc.2020.05.011.
    30. Nikolova, B. et al. (2020). Redox-related Molecular Mechanism of Sensitizing Colon Cancer Cells to Camptothecin Analog SN38. Anticancer Res. 40(9):5159-5170. doi: 10.21873/anticanres.14519.
STA-324 　
    1. Haider, N. et al. (2021). Signaling defects associated with insulin resistance in non-diabetic and diabetic individuals and modification by sex. J Clin Invest. doi: 10.1172/JCI151818.
    2. Ognik, K. et al. (2021). The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine. Sci Rep. 11(1):15975. doi: 10.1038/s41598-021-95529-y.
    3. Kim, J.H. et al. (2021). Nordihydroguaiaretic Acid as a Novel Substrate and Inhibitor of Catechol O-Methyltransferase Modulates 4-Hydroxyestradiol-Induced Cyto- and Genotoxicity in MCF-7 Cells. Molecules. 26(7):2060. doi: 10.3390/molecules26072060.
    4. Psyrri, A. et al. (2021). The DNA damage response network in the treatment of head and neck squamous cell carcinoma. ESMO Open. 6(2):100075. doi: 10.1016/j.esmoop.2021.100075.
    5. Dettleff, P. et al. (2020). Physiological and molecular responses to thermal stress in red cusk-eel (Genypterus chilensis) juveniles reveals atrophy and oxidative damage in skeletal muscle. J Therm Biol. doi: 10.1016/j.jtherbio.2020.102750.
    6. Ognik, K. et al. (2020). The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys. Vet Res. 51(1):50. doi: 10.1186/s13567-020-00776-y.
    7. Huo, X. et al. (2020). In Barrett's Epithelial Cells, Weakly Acidic Bile Salt Solutions Cause Oxidative DNA Damage with Response and Repair Mediated by p38. Am J Physiol Gastrointest Liver Physiol. doi: 10.1152/ajpgi.00329.2019.
    8. Sherwood, T.A. et al. (2019). Nonlethal Biomarkers of Oxidative Stress in Oiled Sediment Exposed Southern Flounder (Paralichthys lethostigma): Utility for Field-Base Monitoring Exposure and Potential Recovery. Environ Sci Technol. 53(24):14734-14743. doi: 10.1021/acs.est.9b05930.
    9. Rivas-Aravena, A. et al. (2019). Transcriptomic response of rainbow trout (Oncorhynchus mykiss) skeletal muscle to Flavobacterium psychrophilum. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 100596. doi:10.1016/j.cbd.2019.100596.
    10. Thai, S.F. et al. (2019). Differential Effects of Nano TiO₂ and CeO₂ on Normal Human Lung Epithelial Cells In Vitro. J Nanosci Nanotechnol. 19(11):6907-6923. doi: 10.1166/jnn.2019.16737.
    11. Norambuena, J. et al. (2019). Superoxide Dismutase and Pseudocatalase Increase Tolerance to Hg(II) in Thermus thermophilus HB27 by Maintaining the Reduced Bacillithiol Pool. MBio. 10(2). pii: e00183-19. doi: 10.1128/mBio.00183-19.
    12. Souliotis, V.L. et al. (2019). DNA damage accumulation, defective chromatin organization and deficient DNA repair capacity in patients with rheumatoid arthritis. Clin Immunol. 203:28-36. doi: 10.1016/j.clim.2019.03.009.
    13. Patchsung, M. et al. (2018). Alu siRNA to increase Alu element methylation and prevent DNA damage. Epigenomics. 10(2):175-185. doi: 10.2217/epi-2017-0096.
    14. Mishra, A. et al. (2018). Oxidative Stress-Mediated Overexpression of Uracil DNA Glycosylase in Leishmania donovani Confers Tolerance against Antileishmanial Drugs. Oxid Med Cell Longev. 2018:4074357. doi: 10.1155/2018/4074357.
    15. Thakur, S., et al. (2017). APE1 modulates cellular responses to organophosphate pesticide-induced oxidative damage in non-small cell lung carcinoma A549 cells. Molecular and Cellular Biochemistry.  441:Issue 1–2, pp 201–216.
    16. Mullick M, et al. (2017). d-Alanine 2, Leucine 5 Enkephaline (DADLE)-mediated DOR activation augments human hUCB-BFs viability subjected to oxidative stress via attenuation of the UPR. Stem Cell Res. 22:20-28. doi: 10.1016/j.scr.2017.05.009.
    17. Periyasamy, M. et al (2017). p53 controls expression of the DNA deaminase APOBEC3B to limit its potential mutagenic activity in cancer cells. Nucleic Acids Research. doi: 10.1093/nar/gkx721.
    18. Stasiolek, M. et al. (2017). The molecular effect of diagnostic absorbed doses from 131I on papillary thyroid cancer cells in vitro. Molecules. doi:10.3390/molecules22060993.
    19. Sapoznik, S. et al. (2016). Activation-induced cytidine deaminase links ovulation-induced inflammation and serous carcinogenesis. Neoplasia. 18:90-99.
    20. Garama, D. J. et al. (2015). A synthetic lethal interaction between glutathione synthesis and mitochondrial reactive oxygen species provides a tumor specific vulnerability dependent on STAT3. Mol Cell Biol. doi:10.1128/MCB.00541-15.
    21. Guzmán-Guillén, R. et al. (2015). Beneficial effects of vitamin E supplementation against the oxidative stress on Cylindrospermopsin-exposed tilapia (Oreochromis niloticus). Toxicon.  104:34-42.
    22. Ferreira, E. et al. (2015). Glyceraldehyde-3-phosphate dehydrogenase is required for efficient repair of cytotoxic DNA lesions in Escherichia coli. Int J Biochem Cell Biol. 60:202-212.
    23. Zhao, K. et al. (2014).  S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair.  EMBO Rep. 15:792-800.
    24. Mohammad, M. K. et al. (2014).  Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice. Biomed Res Int. 2014:512834.
    25. Zafiropoulos, A. et al. (2014).  Cardiotoxicity in rabbits after a low-level exposure to diazinon, propoxur, and chlorpyrifos. Hum Exp Toxicol.33:1241-1252.
    26. Messaoudi, N. et al. (2013). Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism. J.Bacteriol. 195:11
    27. Zaika, E. et al. (2011). p73 Protein Regulates DNA Damage Repair. FASEB J. 25:4406-4414.
STA-321 　
    1. Liu, Q. et al. (2021). Modular Assembly of Tumor-Penetrating and Oligomeric Nanozyme Based on Intrinsically Self-Assembling Protein Nanocages. Adv Mater. doi: 10.1002/adma.202103128.
    2. Tessonnier, T. et al. (2021). FLASH dose-rate helium ion beams: first in vitro investigations. Int J Radiat Oncol Biol Phys. doi: 10.1016/j.ijrobp.2021.07.1703.
    3. Ekuban, A. et al. (2021). Role of Macrophages in Cytotoxicity, Reactive Oxygen Species Production and DNA Damage in 1,2-Dichloropropane-Exposed Human Cholangiocytes In Vitro. Toxics. 9(6):128. Doi: 10.3390/toxics9060128.
    4. Park, J.Y. et al. (2020). Targeted Therapy of Hepatocellular Carcinoma Using Gemcitabine-Incorporated GPC3 Aptamer. Pharmaceutics. 12(10):E985. doi: 10.3390/pharmaceutics12100985.
    5. Zhong, C. et al. (2020). Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno‐associated virus serotype 6 vectors. FASEB J. doi: 10.1096/fj.201902875r.
    6. Yuan, Y. et al. (2020). Deterioration of hematopoietic autophagy is linked to osteoporosis. Aging Cell. doi: 10.1111/acel.13114.
    7. Han, S. et al. (2019). Secretome analysis of patient-derived GBM tumor spheres identifies midkine as a potent therapeutic target. Exp Mol Med. 51(12):147. doi: 10.1038/s12276-019-0351-y.
    8. Zhang, Y. et al. (2019). N-Acetyl Cysteine as a Novel Polymethyl Methacrylate Resin Component: Protection against Cell Apoptosis and Genotoxicity. Oxidative Medicine and Cellular Longevity. doi: 10.1155/2019/1301736.
    9. Wang, Z. et al. (2019). Changes in the Proliferation Rate, Clonogenicity, and Radiosensitivity of Cultured Cells During and After Continuous Low-Dose-Rate Irradiation. Dose Response. 17(2):1559325819842733. doi: 10.1177/1559325819842733.
    10. Izumi, Y. et al. (2019). Suplatast tosilate reduces radiation-induced lung injury in mice through suppression of oxidative stress. Free Radic Biol Med. 136:52-59. doi: 10.1016/j.freeradbiomed.2019.03.024.
    11. Nakade, S. et al. (2018). Biased genome editing using the local accumulation of DSB repair molecules system. Nat Commun. 9(1):3270. doi: 10.1038/s41467-018-05773-6.
    12. Douiev, L. et al. (2018). The pathomechanism of cytochrome c oxidase deficiency includes nuclear DNA damage. Biochim Biophys Acta Bioenerg. 1859(9):893-900. doi: 10.1016/j.bbabio.2018.06.004.
    13. Douiev, L. et al. (2018). Cytochrome c oxidase deficiency, oxidative stress, possible antioxidant therapy and link to nuclear DNA damage. Eur J Hum Genet. 26(4):579-581. doi: 10.1038/s41431-017-0047-5.
    14. Shirasugi, M. et al. (2016). Normal human gingival fibroblasts undergo cytostasis and apoptosis after long-term exposure to butyric acid. Biochem. Biophys. Res. Commun. doi:10.1016/j.bbrc.2016.11.168.
    15. Wu, S. T. et al. (2016). Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells. J Biomed Sci. 23:67.
    16. Cheng, K. P. et al. (2016). Blue light modulates murine microglial gene expression in the absence of optogenetic protein expression. Sci Rep. doi:10.1038/srep21172.
    17. Ohashi, S. et al. (2014). Preclinical validation of talaporfin sodium-mediated photodynamic therapy for esophageal squamous cell carcinoma. PLoS One. 9:e103126.
    18. Dokic, I. et al. (2014). High resistance to X-rays and therapeutic carbon ions in glioblastoma cells bearing dysfunctional ATM associates with intrinsic chromosomal instability. Int J Radiat Biol. 91:157-165.
    19. Zhuge, C. C. et al. (2014). Fullerenol protects retinal pigment epithelial cells from oxidative stress–induced premature senescence via activating SIRT1. Invest Ophthalmol Vis Sci. 55:4628-4638.
    20. Matsuda, S. et al. (2014). An easy-to-use genotoxicity assay using EGFP-MDC1-expressing human cells. Gene Environ. 36:17-28.
    21. Edwards, A.K. et al. (2014).  A peptide inhibitor of synuclein-g reduces neovascularization of human endometriotic lesions.  Mol Hum Reprod. 10.1093/molehr/gau054.

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
전체보기

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
전체보기

자료

웨비나/Video

TOP

제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

로그인

로그인