AXOL 알츠하이머 모델 세포 | 고마바이오텍

특징 

• Human iPSC-derived neural stem cell은 알츠하이머 환자로부터 fibroblast 혹은 PBMC를 채취하여 episomal vector 방법을 통해 iPSC로 리프로그래밍 후 chemically defined cortical neural induction을 이용하여 neural stem cell(NSC)로 분화시켜 만든 신경 줄기 세포 입니다. 
  
• ApoE 혹은 Presenilin 1 유전자 형을 갖도록 엔지니어링되었습니다. 
  
• 배양 21일차 정도에 spontaneous synaptic activity가 감지되며, 35일차에 synchronized burst firing이 발생합니다.
  
• Astrocyte와 함께 co-culture를 함으로써 LTP(long-term potentiation)이나 LTD(long-term depression)을 유도할 수 있습니다. 

사용 논문

• Xia, Y., Wang, Zh., Zhang, J. et al. C/EBPβ is a key transcription factor for APOE and preferentially mediates ApoE4 expression in Alzheimer’s disease. Mol Psychiatry 26, 6002–6022 (2021). https://doi.org/10.1038/s41380-020-00956-4
  
• Koroleva, A. et al. In Vitro Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds. ACS Appl. Mater. Interfaces, 13, 7, 7839–7853(2021). https://pubs.acs.org/doi/10.1021/acsami.0c16616
• Jones, V. et al. Aberrant iPSC-derived human astrocytes in Alzheimer's disease. Cell Death Dis 8, e2696 (2017). https://doi.org/10.1038/cddis.2017.89
• Zollo, A. et al. Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer’s Disease Patients Carrying the APOE Epsilon 4 Allele, Neural Plasticity, vol. 2017, Article ID 1892612, 10 pages, 2017. https://doi.org/10.1155/2017/1892612