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제품 상세
mRNA 항원을 이용한 막단백질 항체 제작 서비스 이미지

mRNA 항원을 이용한 막단백질 항체 제작 서비스

mRNA 항원을 이용한 antibody service로 에피토프 접근이 어려운 transmembrane protein에 대한 항체를 효과적으로 생산할 수 있습니다.

특징 

 

  • 항원으로 mRNA/LNP를 사용하여 막단백질의 epitope에 효과적으로 접근하여 면역원성을 향상시킬 수 있습니다.
  • GPCR, Ion channel, transporter, receptor등 막단백질을 타겟팅하는 항체 생산에 효율적입니다.

 

 

항원별 비교 

 

Immunogen format Weaknesses Strengths Recommended index
mRNA Instability of mRNA, mRNA delivery Direct translation of mRNA in the cytoplasm, spontaneous protein PTM in mammalian cells, natural conformation, no need for adjuvant ★★★★★
DNA low expression level since DNA must cross the nuclear membrane for transcription, high cost for DNA immunization  
Protein High technical limitation of full length GPCRs expression and purification in vitro High immunogenicity ★★
Peptide Only recognize linear epitopes, not conformational epitopes, epitope omission Low synthesis cost ★★★★
VLP Non-specific antibody generation, little difference between positive and negative screening Higher abundance of target antigen compared with overexpressed cells ★★★
Overexpressed cell line Low immunogenicity due to the very low percentage of GPCR of interest on the entire membrane   ★★
Nanodisc Low success rate of GPCRs assembly with MSPs and phospholipid to form Nanodisc Similar to natural cell membrane structure ★★★

 

 

Workflow

 

Case Study

 

Case 1) 7TM protein hCCR9   

  • Goal: Generate mouse monoclonal antibodies specific to 7-transmembrane protein hCCR9
  • Immunogen: hCCR9-mRNA-LNP
  • Screening Material: hCCR9-overexpressing CHO-K1
  • Challenges: No available purified protein & failed in DNA immunization
  • Results: Successfully generate 9 hCCR9-positive clones and 7 validated clones (client selected)

  

 

 

Case 2) 4TM protein hCLD-18.2  

  • Goal: Generate mouse monoclonal antibodies specific to 4-transmembrane protein hCLD-18.2
  • Immunogen: hCLD18.2-mRNA-LNP
  • Screening Material: hCLD18.2-overexpressing CT26
  • Challenges: No available purified protein & failed in DNA immunization
  • Results: Successfully generate 386 hCLD18.2-positive parental clones

 





Case 3) 7TM protein hPTX 
  • Goal: Generate mouse monoclonal antibodies that are specific to 7-transmembrane protein hPTX
  • Immunogen: hPTX-mRNA-LNP
  • Screening Material: hPTX-overexpressing 293FT
  • Challenges: No available purified protein & highly PTM
  • Results: Successfully generate 21 hPTX-positive parental clones