PathHunter Pharmacotrafficking Assay (Pharmacochaperone 분석) | 고마바이오텍

특징

  

• PathHunter cell-based pharmacotrafficking assay는 단백질의 misfolding에 의한 내재화와 protein trafficking에 관련된 질병 과정에서의 약리학적 특성화와 화합물의 기능을 조사하기 위한 cell line을 제공합니다.
• 잘못 접힌 단백질의 적절한 folding을 촉진하고 의도한 위치로의 수송을 지원하여 pharmacochaperone으로 기능하는 화합물의 식별 및 최적화를 지원합니다.

  

 

원리

  

세포주는 ER에 있는 misfolded PK-tagged transmembrane protein과 cell membrane에 있는 EA-tag을 공동발현합니다. Pharmacochaperone이 misfolded PK-tagged protein에 결합하면 단백질이 골지체를 거쳐 세포막으로 이동하고 EA-PK를 형성하여 fuctional enzyme은 substrate를 가수분해하여 chemiluminescent signal을 생성하며, misfolded protein은 endosome으로 내재화 됩니다. 

 

 

 

  

구성

  

• cell line 2 vials

 

 

 

종류

 

Target	PRODUCT TYPE	Item Number	PRODUCT DESCRIPTION	Size
ADRB2(W158A)	Cell Lines	93-0986C3	PathHunter U2OS ADRB2(W158A) ENDO-EA Pharmacotrafficking Assay	2 vials
ADRB2(W158A)	Consumables	493-0986C3	PathHunter U2OS ADRB2(W158A) ENDO-EA Pharmacotrafficking Assay Starter Pack	Pack
AVPR2(S167T)	Cell Lines	93-0992C3	PathHunter U2OS AVPR2(S167T) ENDO-EA Pharmacotrafficking Assay	2 vials
AVPR2(S167T)	Consumables	493-0992C3	PathHunter U2OS AVPR2(S167T) ENDO-EA Pharmacotrafficking Assay Starter Pack	Pack
CFTR-ΔF508	Cell Lines	93-0987C3	PathHunter U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay	2 vials
CFTR-ΔF508	Consumables	493-0987C3	PathHunter U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay Starter Pack	Pack
KCNH2(G601S)	Cell Lines	93-1064C3	PathHunter U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay	2 vials
KCNH2(G601S)	Consumables	493-1064C3	PathHunter U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay Starter Pack	Pack
MC4R(T162I)	Cell Lines	93-1004C3	PathHunter U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay	2 vials
MC4R(T162I)	Consumables	493-1004C3	PathHunter U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay Starter Pack	Pack
mRHO(P23H)	Cell Lines	93-0990C3	PathHunter U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay	2 vials
mRHO(P23H)	Cell Lines	93-1077C3	PathHunter U2OS mRHO(P23H) MEM-EA Pharmacotrafficking Assay	2 vials
mRHO(P23H)	Consumables	493-0990C3	PathHunter U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay Starter Pack	Pack
mRHO(P23H)	Consumables	493-1077C3	PathHunter U2OS mRHO(P23H) MEM-EA Pharmacotrafficking Assay Starter Pack	Pack
SMO(W535L)	Cell Lines	93-0991C3	PathHunter U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay	2 vials
SMO(W535L)	Consumables	493-0991C3	PathHunter U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay Starter Pack	Pack