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레트로바이러스 패키징 | Retrovirus Packaging Plasmid Vector 이미지

레트로바이러스 패키징 | Retrovirus Packaging Plasmid Vector

10A1, Amphotropic, Ecotropic, VSV-G Envelope Vector등의 Retrovirus 생산을 위한 packaging plasmid vector와 Gag-Pol Retroviral Vector를 소개합니다.

프린트 제품 문의

특징

  

  • Retrovirus는 dividing cell의 genome으로 gene을 전달하기 위한 tool로 이용됩니다. 
  • Retrovirus Packaging Plasmid Vector는 Retrovirus Expression Vector와 함께 293T 및 293RTV cell line (#RV-100)에서 MMLV-based retrovirus의 패키징에 이용됩니다. 
  • ecotropic, amphotropic, 10A1 envelop protein은 천연 MMLV 변이체로, 깨지기 쉬우므로 VSVG에 비해 상대적으로 안전합니다. 
  • Envelope protein별로 최적의 host cell range는 다음과 같습니다. 




종류


VSV-G Envelope Vector (#RV-110)
  • pCMVVSV-G는 CMV immediate-early promoter 제어하에 수포성 구내염 바이러스 (vesicular stomatitis virus /VSV-G)의 G glycoprotein을 발현합니다.
  • VSV-G는 바이러스 진입을 매개하여 Moloney Murine Leukenia Virus (MMLV) 기반 retrovirus vector의 pseudotyping에 사용됩니다. 
  • VSV-G는 표적 세포막의 인지질 성분과 상호작용하고 바이러스 및 세포막의 융합을 촉진합니다.
  • VSV-G는 세포 표면 수용체를 필요로 하지 않으며 대리 바이러스 외피 단백질 (surrogate viral envelope protein)로 작용할 수 있습니다.
  • 벡터는 selection marker로 ampicillin-resistance gene을 포함합니다. 
  • 10 µg (0.25 µg/µL in TE)로 제공됩니다. 



Gag-Pol Retroviral Vector (#RV-111)
  • Moloney Murine Leukemia Virus (MMLV)-based retroviral vector system은 가장 일반적으로 사용되는 gene transfer vehicle 입니다.
  • pCMV-Gag-Pol은 CMV mmediate-early promoter의 제어하에 레트로바이러스 구조 단백질을 발현합니다.
  • gag 영역은 capsid protein을 암호화하고, pol 영역은 reverse transcriptase와 integrase 단백질을 암호화 합니다. 

  

 

  

 

사용 논문

 

 

RV-112 　
    1. Fu. R.Y. et al (2020). CD4+ T Cells Engineered with FVIII-CAR and Murine Foxp3 Suppress Anti-Factor VIII Immune Responses in Hemophilia A Mice. Cell Immunol. doi: 10.1016/j.cellimm.2020.104216.
    2. Walia, M.K. et al. (2018). Tolerance to sustained activation of the cAMP/Creb pathway activity in osteoblastic cells is enabled by loss of p53. Cell Death Dis. 9(9):844. doi: 10.1038/s41419-018-0944-8.
    3. Ng, A. J. et al. (2015). The DNA helicase Recql4 is required for normal osteoblast expansion and osteosarcoma formation. PLoS Genet. 10:e1005160.
RV-110 　
    1. Jia, R. et al. (2021). The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. J Biol Chem. doi: 10.1016/j.jbc.2021.100405.
    2. Fu. R.Y. et al (2020). CD4+ T Cells Engineered with FVIII-CAR and Murine Foxp3 Suppress Anti-Factor VIII Immune Responses in Hemophilia A Mice. Cell Immunol. doi: 10.1016/j.cellimm.2020.104216.
    3. Wąchalska, M. et al. (2020). Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles. Membranes (Basel). 10(12):E373. doi: 10.3390/membranes10120373.
    4. Simpson, L.M. et al. (2020). Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System. Cell Chem Biol. S2451-9456(20)30236-1. doi: 10.1016/j.chembiol.2020.06.013.
    5. Tachie-Menson, T. et al. (2020). Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H. Cell Signal. doi: 10.1016/j.cellsig.2020.109632.
    6. Grabowska, K. et al. (2020). Alphaherpesvirus gB Homologs Are Targeted to Extracellular Vesicles, but They Differentially Affect MHC Class II Molecules. Viruses. 12(4). pii: E429. doi: 10.3390/v12040429.
    7. Wu, K.Z.L. et al. (2019). Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling. Wellcome Open Res. 4:133. doi: 10.12688/wellcomeopenres.15403.1.
    8. Wenta, T. et al. (2019). HtrA4 Protease Promotes Chemotherapeutic-Dependent Cancer Cell Death. Cells. 8(10):1112. doi: 10.3390/cells8101112.
    9. Terada, Y. et al. (2019). Human Pluripotent Stem Cell-Derived Tumor Model Uncovers the Embryonic Stem Cell Signature as a Key Driver in Atypical Teratoid/Rhabdoid Tumor. Cell Rep. 26(10):2608-2621.e6. doi: 10.1016/j.celrep.2019.02.009.
    10. Vargas, G. et al. (2019). ERRα promotes breast cancer cell dissemination to bone by increasing RANK expression in primary breast tumors. Oncogene. 38(7):950-964. doi: 10.1038/s41388-018-0579-3.
    11. Hafner-Bratkovič, I. et al. (2018). NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. Nat Commun. 9(1):5182. doi: 10.1038/s41467-018-07573-4.
    12. Macartney, T. J. et al. (2017). An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins. Bio-protocol. 7(22): e2614. doi: 10.21769/BioProtoc.2614.
    13. Sušjan, P. et al. (2017). The mechanism of NLRP3 inflammasome initiation: Trimerization but not dimerization of the NLRP3 pyrin domain induces robust activation of IL-1β. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.01.008.
    14. Yang, W. S. et al. (2016). Peroxidation of polyunsaturated fatty acids by lipoxygenases drives ferroptosis. Proc Natl Acad Sci U S A.  doi:10.1073/pnas.1603244113.
    15. Gallant, J. N. et al. (2015). EGFR kinase domain duplication (EGFR-KDD) is a novel oncogenic driver in lung cancer that is clinically responsive. Cancer Discov. 5:1155-1163.
    16. Zhang, T. et al. (2015). Homoharringtonine binds to and increases myosin-9 in myeloid leukemia. Br J Pharmacol. doi:10.1111/bph.13359.
    17. Amagai, Y. et al. (2015). A point mutation in the extracellular domain of KIT promotes tumorigenesis of mast cells via ligand-independent auto-dimerization. Sci Rep. 5:9775.
    18. Abram, M. E. et al. (2015). A cell-based strategy to assess intrinsic inhibition efficiencies of HIV-1 reverse transcriptase inhibitors. Antimicrob Agents Chemother. 59:838-848.
    19. Gallaher, Z. R. et al. (2014). Neural proliferation in the dorsal root ganglia of the adult rat following capsaicin‐induced neuronal death. J Comp Neurol. 522:3295-3307.
    20. Handorf, A. et al. (2014). Endogenously Produced Indian Hedgehog Regulates TGFβ-Driven Chondrogenesis of Human Bone Marrow Stromal/Stem Cells. Stem Cells Dev. doi:10.1089/scd.2014.0266.
    21. Kobayashi, J. et al. (2014). Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome. PLoS One. 9:e102796.
    22. Higuchi, A. et al. (2014). Preparation of induced pluripotent stem cells on dishes grafted on oligopeptide under feeder-free conditions. J Taiwan Inst Chem E. 45:295-301.
    23. Amagai, Y. et al. (2013). Stem Cell Factor Contributes to Tumorigenesis of Mast Cells via an Autocrine/Paracrine Mechanism.J. Leukoc. Biol. 93:245-250.
    24. Okamoto, K. et al. (2012). Dengue Virus Strain DEN2 16681 Utilizes a Specific Glycochain of Syndecan-2 Proteoglycan as a Receptor. J.Gen. Virol. 93:761-770.
    25. Oida, T. et al. (2010). Overexpression of TGF-ß1 Gene Induces Cell Surface Localized Glucose-Regulated Protein 78-Associated Latency-Associated Peptide/TGF-ß. J. Immunol. 185:3529-3535.
RV-111 　
    1. Crowe, M.S. et al. (2021). RAF-mutant melanomas differentially depend on ERK2 over ERK1 to support aberrant MAPK pathway activation and cell proliferation. Mol Cancer Res. doi: 10.1158/1541-7786.MCR-20-1022.
    2. Jia, R. et al. (2021). The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. J Biol Chem. doi: 10.1016/j.jbc.2021.100405.
    3. Simpson, L.M. et al. (2020). Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System. Cell Chem Biol. S2451-9456(20)30236-1. doi: 10.1016/j.chembiol.2020.06.013.
    4. Tachie-Menson, T. et al. (2020). Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H. Cell Signal. doi: 10.1016/j.cellsig.2020.109632.
    5. Wu, K.Z.L. et al. (2019). Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling. Wellcome Open Res. 4:133. doi: 10.12688/wellcomeopenres.15403.1.
    6. Hennessy, E.J. et al. (2019). The long noncoding RNA CHROME regulates cholesterol homeostasis in primates. Nature Metabolism. 1:98–110. doi: 10.1038/s42255-018-0004-9.
    7. Rajavelu, A. et al. (2018). Chromatin-dependent allosteric regulation of DNMT3A activity by MeCP2. Nucleic Acids Res. 46(17):9044-9056. doi: 10.1093/nar/gky715.
    8. Marazioti, A. et al. (2018). Myeloid-derived interleukin-1β drives oncogenic KRAS-NF-κΒ addiction in malignant pleural effusion. Nat Commun. 9(1):672. doi: 10.1038/s41467-018-03051-z.
    9. Macartney, T. J. et al. (2017). An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins. Bio-protocol. 7(22): e2614. doi: 10.21769/BioProtoc.2614.
    10. Giannou, A.D. et al. (2017). NRAS destines tumor cells to the lungs. EMBO Mol Med. pii: e201606978. doi: 10.15252/emmm.201606978.
    11. Mackay, L. K. et al. (2016). Hobit and Blimp1 instruct a universal transcriptional program of tissue residency in lymphocytes. Science. 352:459-463.
    12. Okamoto, K. et al. (2012). Dengue Virus Strain DEN2 16681 Utilizes a Specific Glycochain of Syndecan-2 Proteoglycan as a Receptor. J.Gen. Virol. 93:761-770.

 

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
pCMV-Gag-Pol Retroviral Vector RV-111 10 ug CELL BIOLABS
pCMV-VSV-G Retroviral Vector RV-110 10 ug CELL BIOLABS
pMXs-miRNA-GFP/Puro Retroviral Expression Vector RTV-017 10 ug CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
RV-111
Product
pCMV-Gag-Pol Retroviral Vector
Size
10 ug
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
RV-110
Product
pCMV-VSV-G Retroviral Vector
Size
10 ug
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
RTV-017
Product
pMXs-miRNA-GFP/Puro Retroviral Expression Vector
Size
10 ug
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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