Bacterial Lipopolysaccharides (LPS) Extraction Kit | 고마바이오텍

특징

• 박테리아 세포에서 LPS를 신속히 추출할 수 있는 Kit입니다.
  
• 여러 그람음성 세균에 적용할 수 있습니다.
• LPS 추출에 60분 정도 소요됩니다.
• LPS 회수율이 높습니다. 
• 구성 : Lysis Buffer, Purification Buffer
• 과정 : Lysis  → LPS purification  → Washing & Elution 

결과  

LPS was extracted from various cells using the ADI kit and LPS tested by SDS-PAGE.

Lane 1: Commercial S. enteritidis LPS

Lane 2: Extracted LPS from S. enteritidis

Lane 3: Commercial E.coli LPS

Lane 4: Extracted LPS from E.coli

Lane 5: Commercial S. enteritidis LPS

Lane 6: Extracted LPS from S. enteritidis

Conclusion: ADI kit extracted LPS with better yields than commercial preparation.

LPS was extracted from Salmonella and the ability to stimulate NOS (nitric oxide synthase) was tested in RAW cell 

at 0 (control), 4, 8, and 12 hrs post stimulation with LPS. GAPDH (control gene) analyses by Northern blot