엑소좀 염색 | Exosome & EV Staining Kit | 고마바이오텍

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SEC-purified MCF-7-derived exosomes were stained with ExoBrite™ EV Membrane Stains. 

ExoBrite™ EV Membrane Stains have less background and more complete exosome staining than other classic dye or competitor dyes. Exosomes were purified from MCF-7 cell supernatant using size exclusion chromatography (SEC). The purified exosomes were stained in PBS with the indicated dyes (top row). The exosome population was gated, with the number showing the percentage of particles falling within the exosome gate. Each dye was also added to filtered PBS (bottom row), to look for dye aggregation and non-specific background. Red arrows indicate these dye aggregates. Lipophilic dyes like DiO show a high number of particles of similar size to exosomes, making them unsuited for exosome staining. CellMask™ also shows an unacceptable amount of dye aggregation falling within the exosome gate. The ExoFlow-ONE™ and ExoGlow™ dyes form aggregates that can mostly be gated away from the true exosomes, but they also show less-complete coverage of exosomes than ExoBrite™ membrane stains.

Purified exosomes stained with ExoBrite™ EV membrane stains

Bead-bound EVs stained with ExoBrite™ EV membrane stains 

[고마블로그 ] Tech Tip: Exosome Isolation and Staining Protocols