엑소좀 염색 | ExoBrite™ True EV Membrane Stains | 고마바이오텍

특징

• pan-EV 염색을 위해 디자인 된 lipophilic membrane dye 입니다.
• PKH, DiO, DiI, DiD와 같은 membrane dye가 용해성이 낮아 EV 응집으로 보일 수 있는 문제점을 해결합니다. 
• Flow 분석에서 비특이적 입자와 EV를 명확하게 구별합니다. 
• 형광 나노입자 추적 분석(fNTA)에서 다른 멤브레인 염색시약에 비해 커버리지가 높아, 거의 모든 EV에 적용 됩니다.
• 다양한 source에서 분리된 EVs 염색에 사용됩니다.
• 항체 염색과 함께 사용할 수 있습니다.
• Pacific Blue®, FITC, PE, APC channels 4가지 색상으로 제공됩니다. 
• 검증 세포주 : MCF-7, J774, U2OS, Jurkat, HeLa, CHO, U937, A549, THP-1, RAW 264.7

종류

실험 결과 

SEC purified MCF-7-derived EVs were stained with three green membrane dyes: ExoBrite™ 515/540 True EV Membrane Stain, PKH67, and Neuro-DiO. The stained EVs were diluted and run on a ZetaView particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 488 nm excitation and 500 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 515/540 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than PKH67 or Neuro-DiO.

SEC purified MCF-7-derived EVs were stained with two orange membrane dyes: ExoBrite™ 555/575 True EV Membrane Stain, and DiI. The stained EVs were diluted and run on a ZetaView particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 520 nm excitation and 550 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 555/575 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than DiI.

Validated EV Sources for ExoBrite™ EV Surface Stains