고마바이오텍(주)
Category 전체보기
전기영동 > 단백질 전기영동 전기영동 > 헥산 전기영동
Cat. No.전체보기

검색 결과가 없습니다.

Product전체보기

검색 결과가 없습니다.

Brand전체보기

검색 결과가 없습니다.

제품 상세
Topoisomerase Nuclear Extraction Kit 이미지

Topoisomerase Nuclear Extraction Kit

Topoisomerase Nuclear Extraction Assay Kit는 Topo 연구를 위해 핵산을 추출할 때 높은 Topoisomerase 활성을 유지한 상태로 추출될 수 있도록 최적화한 nuclear extraction kit 입니다.

특 징

 

  • Topo 연구를 위해 cell에서 핵산을 추출할 때 높은 Topoisomerase 활성을 유지한 상태로 추출될 수 있도록 최적화한  nuclear extraction kit 입니다.
  • 대부분의 animal cell line에 사용 가능하며, 특히 human과 rodent cell에 최적화되어 있습니다.
  • 연구하고자 하는 cell line의 Topo activity 정량이 가능합니다.
  • 예비 drug screening 이나 HTS에 사용할 수 있는 고활성 Topo enzyme을 얻을 수 있습니다.


종 류

 

 

Topoisomerase Type I Nuclear Extraction/Assay Kit (#TG1040-1)


  • Type I topoisomerase (topo I) activity 와 intact 100 kDa polypeptide가 풍부한 nuclear extract를 얻을 수 있는 Extraction Kit 입니다. 
  • 구성 
    •  10x TEMP Buffer
    •  10x TEP Buffer
    •  1 M NaCl for extraction
    •  pHOT1 DNA
    •  Marker DNA, relaxed pHOT1
    •  10x Topo I Reaction Buffer
    •  10x gel loading buffer
    •  10x Proteinase K
    •  10% SDS 


Figure 1. Topo I Reaction Products Resolved on Non-EB and EB Gels.
Reactions (20 ul) were terminated with 1% SDS, digested with proteinase K and extracted with 
CIA. The final volume after addition of Loading Dye was approximately 26 ul. Two agarose
gels (1%) were prepared. The top gel was cast and run in the absence of EB and the bottom
gel cast with 0.5 ug EB/ml and electrophoresed in buffer containing 0.5 ug EB/ml. Gels were
run at 50v for 45-50 min and either stained with EB (non-EB gel) or destained with water (EB
gel) per protocols given above. The data show the positions of nicked open circular (OC) DNA
which is pHOT1 DNA containing at least one single stranded nick (all plasmids have a small
amount of nicked OC DNA). Topoisomers are relaxed DNA forms that resolve after incubating
with Topo I in the absence of any drugs; these topoisomers are fully circular and contain no
single stranded interruptions. The formation of topoisomers is diagnostic for strong Topo I
relaxation activity and demonstrate that the enzyme is showing excellent activity. To see this
result, a non-EB gel should be run. The EB gel (Panel B) is ideal for detecting Topo I cleavage
products, such as nicked open circular DNA. The topo II active drug, VP16, did not influence the
topo I reaction, as expected. Note that supercoiled DNA substrate and relaxed DNA products
are rather poorly resolved in EB gels. In some cases, it is very difficult to demonstrate topo I
catalytic activity in this gel system as a result. For this reason, we recommend using a Non-EB
gel separation in activity assays.




Topoisomerase Type IIa Nuclear Extraction/Assay Kit (#TG1050-1)

  • Topo II polypeptide가 풍부한 nuclear extract를 얻을 수 있는 Extraction Kit 입니다. 
  • 구성 
    • 10x TEMP Buffer
    •  10x TEP Buffer
    •  1 M NaCl for extraction
    •  kDNA 
    •  Decatenated kDNA marker
    •  Linearized kDNA marker  
    •  10x gel loading buffer
    •  10x Proteinase K
    •  10% SDS
    •  10x Topo II Incomplete Assay Buffer A
    •  10x ATP Buffer B


 

Fig. 1.  Typical Topo II reaction products with marker DNAs. A 1% EB containing  agarose  gel  was  run.  
Lane  1:    kDNA  Catenated  DNA  marker.  Lane 2, kDNA digested with Xho1.  Lane 3, kDNA + 4 units topo II with 50 uM Etoposide.  
Lane 4, same as lane 3 without etoposide.  Red star marks the position of a topo II cleavage product (linear DNA).  
This band is not seen in the absence of a topo II poison.


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Topoisomerase Type I Nuclear Extraction/Assay Kit TG1040-1 100 assays TOPOGEN
Topoisomerase Type IIa Nuclear Extraction/Assay Kit TG1050-1 100 assays TOPOGEN
Maker
TOPOGEN
Cat.No.
TG1040-1
Product
Topoisomerase Type I Nuclear Extraction/Assay Kit
Size
100 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1050-1
Product
Topoisomerase Type IIa Nuclear Extraction/Assay Kit
Size
100 assays
Qty

추천제품

자료

  • 분석실험/제작서비스 Vol.2

    분석실험/제작서비스 Vol.2 이미지

    [KOMA]

    약물스크리닝, 치료제개발, 암 연구등에 필요한 분석실험, 제작서비스와 관련 제품에 대한 안내입니다.

웨비나/Video

TOP

제품 문의

0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

로그인

로그인