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Topoisomerase Drug Screening Kit 이미지

Topoisomerase Drug Screening Kit

Topoisomerase I Drug Screening Kit, Topoisomerase II Drug Screening Kit, E. coli Topoisomerase IV Drug Screening Kit는 Drug이 Topoisomerase action에 미치는 영향을 확인하기 위해 사용됩니다.

프린트 제품 문의

Topoisomerase I Drug Screening Kit


  • 특정 지점에서 DNA 결함을 일으키는 매개체를 안정화 시키거나 topoisomerase I의 촉매 활성을 억제시키는 lead compound를 스크리닝 합니다.
  • Kit 구성
    - Supercoiled pHOT-1 DNA
    - Relaxed and nicked plasmid DNA markers
    - 10X Topoisomerase I assay/cleavage buffer
    - Sodium dodecyl sulfate (SDS) termination buffer (10%)
    - 10X gel loading dye
    - Control inhibitor (Camptothecin; lyophilized)
    - Proteinase K
 
Cat. No. Product  Size
TG1018-1 Topoisomerase I Drug Screening Kit (25ug SC DNA) 100 assays
TG1018-1A Topoisomerase I Drug Screening Kit (25ug SC DNA + 100u Human Topo I) 100 assays
TG1018-2 Topoisomerase I Drug Screening Kit (62.5ug SC DNA) 250 assays
TG1018-2A Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 250u Human Topo I) 250 assays
TG1018-3A Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 500u Human Topo I) 250 assays


Topoisomerase II Drug Screening Kit

  • 하나의 high affinity topo II cleavage site가 포함된 DNA substrate (pHOT1)를 사용하여 항균, 항바이러스, 항암 활성을 갖는 Topo II inhibitors를 스크리닝하기에 이상적입니다.
  • Cleavable complexes 형성을 촉진시키거나 Topoisomerase II 활성을 antagonize하는 2종류의 topoisomerase inhibitors 검출이 가능합니다.
  • Kit 구성
    -     Supercoiled DNA [pHOT1 DNA]
    - Markers are linear pHOT1 DNA and open circular DNA
    - 10X Topoisomerase II assay buffer
    - Sodium dodecyl sulfate (10%)
    - 10X Gel loading buffer (bromophenol blue, glycerol)
    - Control inhibitor, Etoposide
    - Proteinase K
 
Cat. No. Product  Size
TG1009-1 Topoisomerase II Drug Screening Kit (25ug SC DNA) 100 assays
TG1009-1A Topoisomerase II Drug Screening Kit (25ug SC DNA + 100u Topo Iia) 100 assays
TG1009-2 Topoisomerase II Drug Screening Kit (62.5ug SC DNA) 250 assays
TG1009-2A Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 250u Topo Iia) 250 assays
TG1009-3A Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 500u Topo Iia) 250 assays


Topoisomerase II Drug Screening Kit (kDNA based)

  • 효소의 활성을 억제시키거나 (Catalytic Inhibitory Compounds, CICs) Cleavable complexes 형성을 촉진시키는 (Interfacial Poisons, IFPs) Topo II effector 검출에 이용됩니다.
  • CICs는 DNA substrate에 대한 접근을 차단하거나 ATPase의 활성 및 효소 회전율을 변경하는 등 많은 단계에서 효소에 영향을 미칠 수 있습니다.
  • IFPs는 DNA에서 resealing step 반응을 차단하는 또 다른 유형의 Inhibitor 입니다.
  • Kit 구성 
    -     kDNA Substrate
    - Decatenated and Linearized kDNA Markers
    - 10X Topoisomerase II assay buffer
    - VP-16 (etoposide)
    - Sodium dodecyl sulfate (10%)
    - 10X Gel loading buffer (bromophenol blue, glycerol)
    - Proteinase K
Figure 1. Use of the Topoisomerase I Drug Kit Reactions indicated above each lane were carried out using 5 units of TopoGEN type I topoisomerase. The reactions were incubated for 30 min at 37 C and stopped with SDS. After digestion with proteinase K (50 ug/ml, 60 min at 37 C), samples were loaded onto a 1% agarose gel. Under these conditions, with excess topo I and camptothecin, significant conversion of supercoiled (form I) DNA to open circular (OC DNA) was observed. It is important to recognize that cleavage assays will consume more enzyme than catalytic (relaxation type) assays. This is because each cleavage event consumes one enzyme molecule; i.e., the reaction requires stoichiometic amounts of topo I.




Figure 2. Use of the Topoisomerase II Drug Screening Kit The reactions indicated above each lane in the figure were carried out using the enzyme provided by TopoGEN and 1 ul of DNA (0.25 ug of form I pHOT-1, as provided in each kit). The reaction products were analyzed on a 1% agarose gel; the running buffer contained 0.5 ug/ml ethidium bromide. The gel was then destained in distilled water prior to photodocumentation. Formation of the cleavable complex (linear DNA species) is clearly visible. Additionally, relaxation of supercoiled DNA substrate (form I pRYG DNA) was inhibited in all reactions that contained VM26.

Cat. No.Product Size
TG1019-1Topoisomerase II Drug Screening Kit (20ug kDNA)100 assays
TG1019-1ATopoisomerase II Drug Screening Kit (20ug kDNA + 100u Human Topo Iia)100 assays
TG1019-2Topoisomerase II Drug Screening Kit (50ug kDNA)250 assays
TG1019-2ATopoisomerase II Drug Screening Kit (50ug kDNA + 250u  Human Topo Iia)250 assays
TG1019-3ATopoisomerase II Drug Screening Kit (50ug kDNA + 500u  Human Topo Iia)250 assays


E. coli Topoisomerase IV Drug Screening Kit 

 

  • Cleavable complexes 형성을 촉진시키거나 Topoisomerase IV의 활성에 antagonize하는 2종류의 topoisomerase inhibitors 검출이 가능합니다.
  • 이미 알려져 있는 Topo II poison인 Ciprofloxacin이 control로써 제공됩니다.
  • Kit 구성
    - pHOT1 DNA, supercoiled
    - Marker DNA, Linear pHOT1
    - Incomplete Buffer A
    - ATP Buffer B contains 100 mM ATP
    - Ciprofloxacin control
    - 10% sodium dodecyl sulfate (SDS)
    - 10x gel loading buffer
    - Proteinase K

Cat. No.Product Size
TG1007-1E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)100 assays
TG1007-1AE. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA + 100u EC Topo IV)100 assays
TG1007-2E. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA)250 assays
TG1007-2AE. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA + 250u EC Topo IV)250 assays
TG1007-3AE. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA + 500u EC Topo IV)250 assays


Topoisomerase I Hi-Loading Inhibition Kit

 

  • Human topoisomerase I (topo I)의 촉매 활성을 차단하는 물질 (약물, 천연물, 소분자 화합물..)을 스크리닝 할 수 있도록 설계되었습니다.
  • Kit 구성
    - pHOT1 DNA, supercoiled substrate 
    - Marker DNA, Relaxed pHOT1
    - Topo I reaction buffer 
    - 10% Sodium Dodecyl Sulfate 
    - 10x gel loading buffer 
    - Proteinase K 
    - Purified human topoisomerase I provided at 25 units/ul (20ul)

Cat. No. Product  Size
TG1017-1 Topoisomerase I Hi-Loading Inhibition Kit 500 units
TG1017-2 Topoisomerase I Hi-Loading Inhibition Kit 1000 units

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
E. coli Topo IV Drug Screening Kit (20ug kDNA + 100u E. coli Topo IV) TG1008-1A 100 assays TOPOGEN
E. coli Topo IV Drug Screening Kit (20ug kDNA) TG1008-1 100 assays TOPOGEN
E. coli Topo IV Drug Screening Kit (50ug kDNA + 250u E. coli Topo IV) TG1008-2A 250 assays TOPOGEN
E. coli Topo IV Drug Screening Kit (50ug kDNA + 500u E. coli Topo IV) TG1008-3A 250 assays TOPOGEN
E. coli Topo IV Drug Screening Kit (50ug kDNA) TG1008-2 250 assays TOPOGEN
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA + 250u EC Topo IV) TG1007-2A 250 assays TOPOGEN
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA + 500u EC Topo IV) TG1007-3A 250 assays TOPOGEN
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA) TG1007-2 250 assays TOPOGEN
E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA + 100u EC Topo IV) TG1007-1A 100 assays TOPOGEN
E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA) TG1007-1 100 assays TOPOGEN
Topoisomerase I Drug Screening Kit (25ug SC DNA + 100u Human Topo I) TG1018-1A 100 assays TOPOGEN
Topoisomerase I Drug Screening Kit (25ug SC DNA) TG1018-1 100 assays TOPOGEN
Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 250u Human Topo I) TG1018-2A 250 assays TOPOGEN
Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 500u Human Topo I) TG1018-3A 250 assays TOPOGEN
Topoisomerase I Drug Screening Kit (62.5ug SC DNA) TG1018-2 250 assays TOPOGEN
Topoisomerase I Hi-Loading Inhibition Kit TG1017-2 1000 units TOPOGEN
Topoisomerase I Hi-Loading Inhibition Kit TG1017-1 500 units TOPOGEN
Topoisomerase II Drug Screening Kit (20ug kDNA + 100u Human Topo Iia) TG1019-1A 100 assays TOPOGEN
Topoisomerase II Drug Screening Kit (20ug kDNA) TG1019-1 100 assays TOPOGEN
Topoisomerase II Drug Screening Kit (25ug SC DNA + 100u Topo Iia) TG1009-1A 100 assays TOPOGEN
Topoisomerase II Drug Screening Kit (25ug SC DNA) TG1009-1 100 assays TOPOGEN
Topoisomerase II Drug Screening Kit (50ug kDNA + 250u Human Topo Iia) TG1019-2A 250 assays TOPOGEN
Topoisomerase II Drug Screening Kit (50ug kDNA + 500u Human Topo Iia) TG1019-3A 250 assays TOPOGEN
Topoisomerase II Drug Screening Kit (50ug kDNA) TG1019-2 250 assays TOPOGEN
Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 250u Topo Iia) TG1009-2A 250 assays TOPOGEN
Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 500u Topo Iia) TG1009-3A 250 assays TOPOGEN
Topoisomerase II Drug Screening Kit (62.5ug SC DNA) TG1009-2 250 assays TOPOGEN
전체보기
Maker
TOPOGEN
Cat.No.
TG1008-1A
Product
E. coli Topo IV Drug Screening Kit (20ug kDNA + 100u E. coli Topo IV)
Size
100 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1008-1
Product
E. coli Topo IV Drug Screening Kit (20ug kDNA)
Size
100 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1008-2A
Product
E. coli Topo IV Drug Screening Kit (50ug kDNA + 250u E. coli Topo IV)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1008-3A
Product
E. coli Topo IV Drug Screening Kit (50ug kDNA + 500u E. coli Topo IV)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1008-2
Product
E. coli Topo IV Drug Screening Kit (50ug kDNA)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1007-2A
Product
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA + 250u EC Topo IV)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1007-3A
Product
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA + 500u EC Topo IV)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1007-2
Product
E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA)
Size
250 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1007-1A
Product
E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA + 100u EC Topo IV)
Size
100 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1007-1
Product
E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)
Size
100 assays
Qty
Maker
TOPOGEN
Cat.No.
TG1018-1A
Product
Topoisomerase I Drug Screening Kit (25ug SC DNA + 100u Human Topo I)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1018-1
Product
Topoisomerase I Drug Screening Kit (25ug SC DNA)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1018-2A
Product
Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 250u Human Topo I)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1018-3A
Product
Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 500u Human Topo I)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1018-2
Product
Topoisomerase I Drug Screening Kit (62.5ug SC DNA)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1017-2
Product
Topoisomerase I Hi-Loading Inhibition Kit
Size
1000 units
Qty
Maker
TOPOGEN
Cat.No.
TG1017-1
Product
Topoisomerase I Hi-Loading Inhibition Kit
Size
500 units
Qty
Maker
TOPOGEN
Cat.No.
TG1019-1A
Product
Topoisomerase II Drug Screening Kit (20ug kDNA + 100u Human Topo Iia)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1019-1
Product
Topoisomerase II Drug Screening Kit (20ug kDNA)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1009-1A
Product
Topoisomerase II Drug Screening Kit (25ug SC DNA + 100u Topo Iia)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1009-1
Product
Topoisomerase II Drug Screening Kit (25ug SC DNA)
Size
100 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1019-2A
Product
Topoisomerase II Drug Screening Kit (50ug kDNA + 250u Human Topo Iia)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1019-3A
Product
Topoisomerase II Drug Screening Kit (50ug kDNA + 500u Human Topo Iia)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1019-2
Product
Topoisomerase II Drug Screening Kit (50ug kDNA)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1009-2A
Product
Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 250u Topo Iia)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1009-3A
Product
Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 500u Topo Iia)
Size
250 assays
Qty
Data Sheet
Maker
TOPOGEN
Cat.No.
TG1009-2
Product
Topoisomerase II Drug Screening Kit (62.5ug SC DNA)
Size
250 assays
Qty
Data Sheet
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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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