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제품 상세
아데노바이러스 정제 | Adenovirus Purification Kit 이미지

아데노바이러스 정제 | Adenovirus Purification Kit

ViraBind™ Adenovirus Miniprep Kit와 PureVirus™ Adenovirus Purification Kit는 CsCl을 이용한 ultracentrifugation 과정없이 2시간 이내에 아데노바이러스를 정제하며, 회수율이 높습니다.

프린트 제품 문의

ViraBind™ Adenovirus Miniprep Kit (#VPK-099) 

  

  • Adenovial capsid 단백질을 column으로 분리합니다.
  • CsCl 초원심분리 과정과 CsCl을 분리할 필요가 없어 시간이 단축되고 번거롭지 않습니다. 
  • 30분 내에 concentration이 완료됩니다.(CsCl gradient 시 16-20시간 소요)
  • 2.5X10E12(VPs) 이상 농축이 가능합니다.
  • 회수율 : > 95% 
  • 구성 : 
    -     ViraBind™ Columns and Collection tubes
    - Loading Buffer
    - Wash Buffer


Purification of recombinant Ad-β Gal. Recombinant Ad-β Gal viruses were purified according to the Purification Instructions. Each fraction, obtained during purification, and its dilution were used to infect A549 cells in a 12-well plate. After 48 hrs, X-gal staining was performed and β Gal positive cells (blue) were scored. 



Membrane Ruffling Induced by Activated H-Ras. COS-7 cells were infected with purified Ad-β Gal or Ras G12V at 50 MOI (multiplicity of infection). X-gal staining was performed after 48 hr infection period. Membrane ruffling is visualized by staining the actin cytoskeleton with Rhodamine-coupled Phalloidin.




PureVirus™ Adenovirus Purification Kit (#VPK-5112)

  • 전용 resin을 이용하여, adenoviral supernatants에 있는 contaminants들을 98% 이상 제거하여 adenovirus의 회수율을 높였습니다.
  • 회수율 : > 60-70% 
  • 구성 : 
    - Virus Purification Resin
    - Virus Pretreatment Solution
    - Virus Purification Buffer
    - Microspin Columns


Purification of recombinant Ad-β Gal. Recombinant Ad-β Gal viruses were purified according to the Purification Protocol. The adenoviral infectivity of each fraction obtained during purification was determined through Hexon staining using QuickTiter™ Adenovirus Titer Immunoassay Kit (Cat. #VPK-109). Top Left: Viral Lysate Supernatant; Bottom Left: Purified Adenovirus Sample. Removal of host cell protein and nucleic acid contaminants were monitored through BCA protein assay and CyQuant DNA/RNA assay. More than 98% of both host cell proteins and nucleic acids were removed in the final purified adenovirus sample (data not shown).


사용 논문

VPK-099 　
    1. Pang, L. et al. (2021). Attenuation and transport of human enteric viruses and bacteriophage MS2 in alluvial sand and gravel aquifer media-laboratory studies. Water Res. 196:117051. doi: 10.1016/j.watres.2021.117051
    2. Watanabe, K. et al. (2021). ILDR2 stabilization is regulated by its interaction with GRP78. Sci Rep. 11(1):8414. doi: 10.1038/s41598-021-87884-7.
    3. Teves, M.E. et al. (2020). Human DENND1A.V2 Drives Cyp17a1 Expression and Androgen Production in Mouse Ovaries and Adrenals. Int J Mol Sci. 21(7). pii: E2545. doi: 10.3390/ijms21072545.
    4. Watanabe, K. et al. (2019). Kbtbd11 contributes to adipocyte homeostasis through the activation of upstream stimulatory factor 1. Heliyon. doi: 10.1016/j.heliyon.2019.e02777.
    5. Lochbaum, R. et al. (2019). Retinoic acid signalling adjusts tight junction permeability in response to air-liquid interface conditions. Cell Signal. doi: 10.1016/j.cellsig.2019.109421.
    6. Watanabe, K. et al. (2019). A novel upstream transcription factor 1 target gene N4bp2l1 that regulates adipogenesis. Biochemistry and Biophysics Reports. doi: 10.1016/j.bbrep.2019.100676.
    7. Putranto, E.W. et al. (2017). Expression of tumor suppressor REIC/Dkk-3 by a newly improved adenovirus vector with insertion of a hTERT promoter at the 3'-side of the transgene. Oncology Letters. 14:1041-1048. doi: 10.3892/ol.2017.6201.
    8. Xie, Y. et al. (2016). A recombinant adenovirus expressing P12A and 3C protein of the type O foot-and-mouth disease virus stimulates systemic and mucosal immune responses in mice. BioMed Res Int. doi:10.1155/2016/7849203.
    9. Morris, S. J. et al. (2015). Laboratory-scale production of replication-deficient adenovirus vectored vaccines. Vaccine Technol Vet Viral Disease. doi:10.1007/978-1-4939-3008-1_8.
    10. Müller, J. et al. (2015). TRIM21, a negative modulator of LFG in breast carcinoma MDA-MB-231 cells in vitro. Int J Oncol. 47:1634-1646.
    11. Zhu, L. et al. (2015). Inhibition of porcine reproductive and respiratory syndrome virus replication with exosome-transferred artificial microRNA targeting the 3′ untranslated region. J Virol Methods. 223:61-68.
    12. Boehme, P. et al. (2015). Standard free droplet digital polymerase chain reaction as a new tool for the quality control of high-capacity adenoviral vectors in small-scale preparations. Hum Gene Ther Methods. 26:25-34.
    13. Zhu, L. et al. (2014). Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus-and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors. Virol J. 11:225.
    14. Chen, Y. et al. (2014). Additive inhibition of porcine reproductive and respiratory syndrome virus infection with the soluble sialoadhesin and CD163 receptors. Virus Res. 179:85-92.
    15. McAllister, J.M. et al. (2014). Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca pehontype. PNAS 111:E1519-E1527.
    16. Wilkins, H. et al. (2013). Mitochondrial Glutathione Transport Is a Key Determinant of Neuronal Susceptibility to Oxidative and Nitrosative Stress. J. Biol. Chem. 288:5091-5101.
    17. Wang, Y.S. et al. (2012). MicroRNA-195 Regulates Vascular Smooth Muscle Cell Phenotype and Prevents Neointimal Formation. Cardiovasc Res. 95:517-526.
    18. Kirui, J.K. et al. (2010). Gßgamma Signaling Promotes Breast Cancer Cell Migration and Invasion. J. Pharmacol. Exp. Ther. 333:393-403.
VPK-5112 　
    1. Das, D. et al. (2021). Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPARγ binodal complementary modules. Redox Biol. doi: 10.1016/j.redox.2021.102043.
    2. Daniel, P.V. et al. (2021). NF-κB p65 regulates hepatic lipogenesis by promoting nuclear entry of ChREBP in response to a high carbohydrate diet. J Biol Chem. doi: 10.1016/j.jbc.2021.100714.
    3. Cho, J.H. et al. (2020). CD9 induces cellular senescence and aggravates atherosclerotic plaque formation. Cell Death Differ. doi: 10.1038/s41418-020-0537-9.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
PureVirus™ Adenovirus Purification Kit VPK-5112 10 preps CELL BIOLABS
ViraBind™ Adenovirus Miniprep Kit VPK-099 10 preps CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
VPK-5112
Product
PureVirus™ Adenovirus Purification Kit
Size
10 preps
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
VPK-099
Product
ViraBind™ Adenovirus Miniprep Kit
Size
10 preps
Qty
Data Sheet
MSDS

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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Cat.No.
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Data Sheet
MSDS

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